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The map label for this gene is miaA

Identifier: 91789072

GI number: 91789072

Start: 3398394

End: 3399395

Strand: Direct

Name: miaA

Synonym: Bpro_3212

Alternate gene names: 91789072

Gene position: 3398394-3399395 (Clockwise)

Preceding gene: 91789071

Following gene: 91789073

Centisome position: 65.35

GC content: 64.67

Gene sequence:

>1002_bases
ATGAACGAAGTGAAAAGCGTGGGGGCCAAGTACATTGCACTCACCGGGCCAACAGCGTCGGGCAAGACGGCTGCGGCCAT
GGCCATCGCGCAGCAGCATGACGTCGAGATCATCAGCGTCGATTCAGCGCTGGTCTACCGCGGCATGGACATCGGCACCG
CCAAACCCACGGTCGATGAACTCGCGGCCGTGCCGCATCACCTGATCAACATCCGCGACCCGCTGCAGGCCTACAGTGCG
GCCGAATTCGTGGCCGATGCCCAGCGGCTGATCGACGACATCGCCGCGCGCGGCAAGCTGCCGCTGCTGGTGGGCGGCAC
CATGCTGTATTTCAAGGCTCTCTTTTACGGCCTGGACGACATGCCCAAAGCCGACCCGGCGGTGCGAGCCGAGCTGGCCA
GCGAAGCCGCCGCCAAAGGCTGGCCTGCGCTGCATGCCGAGCTGGCAACCGTTGACCCGGTCACCGCCGCCCGGCTGGCA
CCCCATGATTCACAACGCATCTCGCGCGCGCTGGAAGTGTTCCGGGTGTCCGGGCAGCCGCTGTCGTTCTTTCACCAGCA
AAACGCTGCAAAAACCATAGCTGATGACGGTCGGGAAGAAAGGACTGAAATCCTTATTTCCCTTGAGCCTCAAGAGCGCA
GCTGGCTGCACCACCGCATTGCCGAGCGCTTTGACGCGATGCTGGCGGCCGGCTTTGTCGAGGAAGTCAAAACCCTGCGC
GCGCGCGGGGACCTGACGCCGGACTTGCCGTCCATGCGCTGCGTTGGCTACCGCCAGGCCTGGGAACTGCTTGACGCGCA
GGAGGCGCGCAGCCCCGGCGGCAGCTTCCCGATGGACGAGCTGCGCGACAAGGGCATCATTGCCACGCGCCAGTTGGCCA
AGCGCCAGGTCACCTGGCTGCGCTCCATGCCGCAGCGCCAGATCATCACCTGCGATACCGACCAGGCGCTGCCGCTGGTT
CTGCAGGCAGTGGCTCAACACATCGAAAAGTCCAGCCGATAG

Upstream 100 bases:

>100_bases
CCTCGGGGCTGGAGCCTGCGGCTCCAAGTCTGCTTGAGCAGGCTTGGACAGGCGACAGAGGCGAAGCATCTGGCGAGCCG
CGGCCTGCAAGGCCTCGCGC

Downstream 100 bases:

>100_bases
CCGGCTTGCTTGTGGTGACCGCCGGTGCCAGAATGGGGCGCGCGTGCGGCGCGCGAGTCTTGCCTGCCTCAAGTTATAAC
AACCAAGGAGCGCGCATCAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNEVKSVGAKYIALTGPTASGKTAAAMAIAQQHDVEIISVDSALVYRGMDIGTAKPTVDELAAVPHHLINIRDPLQAYSA
AEFVADAQRLIDDIAARGKLPLLVGGTMLYFKALFYGLDDMPKADPAVRAELASEAAAKGWPALHAELATVDPVTAARLA
PHDSQRISRALEVFRVSGQPLSFFHQQNAAKTIADDGREERTEILISLEPQERSWLHHRIAERFDAMLAAGFVEEVKTLR
ARGDLTPDLPSMRCVGYRQAWELLDAQEARSPGGSFPMDELRDKGIIATRQLAKRQVTWLRSMPQRQIITCDTDQALPLV
LQAVAQHIEKSSR

Sequences:

>Translated_333_residues
MNEVKSVGAKYIALTGPTASGKTAAAMAIAQQHDVEIISVDSALVYRGMDIGTAKPTVDELAAVPHHLINIRDPLQAYSA
AEFVADAQRLIDDIAARGKLPLLVGGTMLYFKALFYGLDDMPKADPAVRAELASEAAAKGWPALHAELATVDPVTAARLA
PHDSQRISRALEVFRVSGQPLSFFHQQNAAKTIADDGREERTEILISLEPQERSWLHHRIAERFDAMLAAGFVEEVKTLR
ARGDLTPDLPSMRCVGYRQAWELLDAQEARSPGGSFPMDELRDKGIIATRQLAKRQVTWLRSMPQRQIITCDTDQALPLV
LQAVAQHIEKSSR
>Mature_333_residues
MNEVKSVGAKYIALTGPTASGKTAAAMAIAQQHDVEIISVDSALVYRGMDIGTAKPTVDELAAVPHHLINIRDPLQAYSA
AEFVADAQRLIDDIAARGKLPLLVGGTMLYFKALFYGLDDMPKADPAVRAELASEAAAKGWPALHAELATVDPVTAARLA
PHDSQRISRALEVFRVSGQPLSFFHQQNAAKTIADDGREERTEILISLEPQERSWLHHRIAERFDAMLAAGFVEEVKTLR
ARGDLTPDLPSMRCVGYRQAWELLDAQEARSPGGSFPMDELRDKGIIATRQLAKRQVTWLRSMPQRQIITCDTDQALPLV
LQAVAQHIEKSSR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=319, Percent_Identity=30.7210031347962, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1790613, Length=317, Percent_Identity=52.6813880126183, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI25144712, Length=299, Percent_Identity=27.4247491638796, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=305, Percent_Identity=26.8852459016393, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24649830, Length=332, Percent_Identity=25.3012048192771, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_POLSJ (Q128B6)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550024.1
- ProteinModelPortal:   Q128B6
- SMR:   Q128B6
- STRING:   Q128B6
- GeneID:   4014136
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3212
- NMPDR:   fig|296591.1.peg.4750
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   LPYTVAN
- PhylomeDB:   Q128B6
- ProtClustDB:   PRK00091
- BioCyc:   PSP296591:BPRO_3212-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36518; Mature: 36518

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVKSVGAKYIALTGPTASGKTAAAMAIAQQHDVEIISVDSALVYRGMDIGTAKPTVDE
CCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEECHHHHHCCCCCCCCCCCHHH
LAAVPHHLINIRDPLQAYSAAEFVADAQRLIDDIAARGKLPLLVGGTMLYFKALFYGLDD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCC
MPKADPAVRAELASEAAAKGWPALHAELATVDPVTAARLAPHDSQRISRALEVFRVSGQP
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCC
LSFFHQQNAAKTIADDGREERTEILISLEPQERSWLHHRIAERFDAMLAAGFVEEVKTLR
HHHHHHHHHHHHHHCCCHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGDLTPDLPSMRCVGYRQAWELLDAQEARSPGGSFPMDELRDKGIIATRQLAKRQVTWL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
RSMPQRQIITCDTDQALPLVLQAVAQHIEKSSR
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEVKSVGAKYIALTGPTASGKTAAAMAIAQQHDVEIISVDSALVYRGMDIGTAKPTVDE
CCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEECHHHHHCCCCCCCCCCCHHH
LAAVPHHLINIRDPLQAYSAAEFVADAQRLIDDIAARGKLPLLVGGTMLYFKALFYGLDD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCC
MPKADPAVRAELASEAAAKGWPALHAELATVDPVTAARLAPHDSQRISRALEVFRVSGQP
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCC
LSFFHQQNAAKTIADDGREERTEILISLEPQERSWLHHRIAERFDAMLAAGFVEEVKTLR
HHHHHHHHHHHHHHCCCHHCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGDLTPDLPSMRCVGYRQAWELLDAQEARSPGGSFPMDELRDKGIIATRQLAKRQVTWL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
RSMPQRQIITCDTDQALPLVLQAVAQHIEKSSR
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA