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The map label for this gene is paaF [C]

Identifier: 91788971

GI number: 91788971

Start: 3284062

End: 3284856

Strand: Direct

Name: paaF [C]

Synonym: Bpro_3111

Alternate gene names: 91788971

Gene position: 3284062-3284856 (Clockwise)

Preceding gene: 91788970

Following gene: 91788972

Centisome position: 63.15

GC content: 65.03

Gene sequence:

>795_bases
ATGACTTCCAACACCTCTTCCACCGACATCCAGCTAGACATTCGCGGCGCGCATCAAGAGATCGCAGTGATCCGCCTGAG
CCGCGTTGCCAAACGTAATGCCGTCAACGACAGCCTGATTCTGGCCTTGCGTGACATCTTCGAAAAACTGCCCACAACCG
TCCGTGCTGCCGTGATCCACGGCGAGGGCGAGCACTTCTGCGCCGGCCTGGACCTGGGCGAACTGGAGGAGCGCGACGCC
GGCCAGGGCATGCACCATTCGCGCATGTGGCACGCCGCGCTCGAACGGGTGCAGTTCGGCCCGGTTCCGGTGATTGCCGC
GCTGCAAGGCGCCGTGGTCGGCGGCGGACTAGAGCTGGCCAGCGCCTGCCATATCCGCGTAGCCGATGAGACTGCCTTCT
ATGCGCTGCCGGAGGGCTCTCGCGGCATTTTTGTCGGCGGTGGCGGATCGGTTCGCATCCCCAAGCTGATAGGCGTCGCA
CGCATGACCGACATGATGCTGACCGGCCGGGTCTACAACGCACAAGACGGCGAGCGCGCCGGCTTTGCGCAGTACCTCGT
ACCCGAGGGCCAAGCCTTCGACAAGGCCATGGAACTGGCCGCACGTGTGGCGCAAAACGCCCCCCTGACGAATTACGCAC
TGATGCATGCCCTGCCCCGGATTGCCGAGCAGCCGGCCGAGCAGGGCTACCTGACGGAAGCCCTGATGGCGGCCATTGCC
CAAAGCGCGCCTGAGGCCAAGGACCGGGTCCGCGCCTTCCTCGAAGGCCGGGCTGCCAAGGTGCAGAAAAGCTGA

Upstream 100 bases:

>100_bases
TTCTTATGGCATGCGGGTTAACACCGATAAAACAGGCTGAAAAGGCAATTGAATTAGTTATGTTTTATAACCACAATTCA
CCCACCAACCAAAGCTGACC

Downstream 100 bases:

>100_bases
AGCCGCGCGGCCCAGCACGGAACGACATATCACCCCATCACGCAACAAGCACAACAAGAAAAACGCGAGCACGCCCGGAA
GAGACAAGCATGACCAGCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTSNTSSTDIQLDIRGAHQEIAVIRLSRVAKRNAVNDSLILALRDIFEKLPTTVRAAVIHGEGEHFCAGLDLGELEERDA
GQGMHHSRMWHAALERVQFGPVPVIAALQGAVVGGGLELASACHIRVADETAFYALPEGSRGIFVGGGGSVRIPKLIGVA
RMTDMMLTGRVYNAQDGERAGFAQYLVPEGQAFDKAMELAARVAQNAPLTNYALMHALPRIAEQPAEQGYLTEALMAAIA
QSAPEAKDRVRAFLEGRAAKVQKS

Sequences:

>Translated_264_residues
MTSNTSSTDIQLDIRGAHQEIAVIRLSRVAKRNAVNDSLILALRDIFEKLPTTVRAAVIHGEGEHFCAGLDLGELEERDA
GQGMHHSRMWHAALERVQFGPVPVIAALQGAVVGGGLELASACHIRVADETAFYALPEGSRGIFVGGGGSVRIPKLIGVA
RMTDMMLTGRVYNAQDGERAGFAQYLVPEGQAFDKAMELAARVAQNAPLTNYALMHALPRIAEQPAEQGYLTEALMAAIA
QSAPEAKDRVRAFLEGRAAKVQKS
>Mature_263_residues
TSNTSSTDIQLDIRGAHQEIAVIRLSRVAKRNAVNDSLILALRDIFEKLPTTVRAAVIHGEGEHFCAGLDLGELEERDAG
QGMHHSRMWHAALERVQFGPVPVIAALQGAVVGGGLELASACHIRVADETAFYALPEGSRGIFVGGGGSVRIPKLIGVAR
MTDMMLTGRVYNAQDGERAGFAQYLVPEGQAFDKAMELAARVAQNAPLTNYALMHALPRIAEQPAEQGYLTEALMAAIAQ
SAPEAKDRVRAFLEGRAAKVQKS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=30.3149606299213, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI68989263, Length=225, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI70995211, Length=213, Percent_Identity=28.169014084507, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI20127408, Length=256, Percent_Identity=28.90625, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=30.1526717557252, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=26.0330578512397, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=31.1475409836066, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI221142681, Length=232, Percent_Identity=33.1896551724138, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788682, Length=147, Percent_Identity=34.6938775510204, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790281, Length=128, Percent_Identity=30.46875, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=214, Percent_Identity=32.2429906542056, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=215, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=28.0155642023346, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=29.7619047619048, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=30.635838150289, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=157, Percent_Identity=33.7579617834395, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=157, Percent_Identity=33.7579617834395, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=157, Percent_Identity=33.7579617834395, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=250, Percent_Identity=26.8, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=266, Percent_Identity=24.0601503759398, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=28.1368821292776, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=28.1368821292776, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=31.1538461538462, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21357171, Length=203, Percent_Identity=30.0492610837438, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19920382, Length=215, Percent_Identity=28.8372093023256, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24650670, Length=204, Percent_Identity=29.9019607843137, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921000, Length=170, Percent_Identity=36.4705882352941, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24583077, Length=170, Percent_Identity=36.4705882352941, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24583079, Length=170, Percent_Identity=36.4705882352941, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28308; Mature: 28177

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNTSSTDIQLDIRGAHQEIAVIRLSRVAKRNAVNDSLILALRDIFEKLPTTVRAAVIH
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEEEEE
GEGEHFCAGLDLGELEERDAGQGMHHSRMWHAALERVQFGPVPVIAALQGAVVGGGLELA
CCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
SACHIRVADETAFYALPEGSRGIFVGGGGSVRIPKLIGVARMTDMMLTGRVYNAQDGERA
CEEEEEEECCCEEEECCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHCEEECCCCCCCC
GFAQYLVPEGQAFDKAMELAARVAQNAPLTNYALMHALPRIAEQPAEQGYLTEALMAAIA
CHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHH
QSAPEAKDRVRAFLEGRAAKVQKS
HCCCHHHHHHHHHHHCHHHHCCCC
>Mature Secondary Structure 
TSNTSSTDIQLDIRGAHQEIAVIRLSRVAKRNAVNDSLILALRDIFEKLPTTVRAAVIH
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEEEEE
GEGEHFCAGLDLGELEERDAGQGMHHSRMWHAALERVQFGPVPVIAALQGAVVGGGLELA
CCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHH
SACHIRVADETAFYALPEGSRGIFVGGGGSVRIPKLIGVARMTDMMLTGRVYNAQDGERA
CEEEEEEECCCEEEECCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHCEEECCCCCCCC
GFAQYLVPEGQAFDKAMELAARVAQNAPLTNYALMHALPRIAEQPAEQGYLTEALMAAIA
CHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHH
QSAPEAKDRVRAFLEGRAAKVQKS
HCCCHHHHHHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA