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The map label for this gene is lutR [H]

Identifier: 91788970

GI number: 91788970

Start: 3282772

End: 3283578

Strand: Direct

Name: lutR [H]

Synonym: Bpro_3110

Alternate gene names: 91788970

Gene position: 3282772-3283578 (Clockwise)

Preceding gene: 91788959

Following gene: 91788971

Centisome position: 63.13

GC content: 63.94

Gene sequence:

>807_bases
ATGACCCTCAGGAAAACCCTTAGTCATCCCGAGCCCCTGCCCGCGCGCCACCTGGCGGCGCCAGGCGCACGCCGTCCGCG
CGGACTGGTCGGTGAAATCGTCGAAAGCCTGGCCAACAGCATTCGCGATGGCCAGTTGCAGCCCGGCGCGAAGCTGCCGA
CAGAAGCAGAAATCATGGCCCGGTTCGATGTCAGCCGCACGGTGGTTCGCGAATCGCTGTCGCGGCTTCAGGCGTCCGGC
CTGGTCGAGACCCGGCATGGCATCGGCACCTTTGTACTGCCCCCGCAGGAAAGCGGCAACTTCAGGATCATGGCGGAAGA
CTTCGCCACGGTAGCCGATGTCATTTCAGTGCTCGAGTTGCGCATCAGCCTTGAAACCGAGGCCGCAGGCCTGGCGGCCC
AGAGGAGAACGCCCGAGAACCTGCTGGCCATGCAAAATGCACTGCGTGCCTTTCGGGAGTCCATCAATGATGACTCCGAC
GCCGTGCCGCCCGACTTCCAGTTTCACATGGAAGTGGCCCGCTCCACAGGCAACCGGCATTTCGCCGACCTGATGACCTA
CCTGGGCACCATGATCATTCCGCGCACGCGCGTGAACATCGTGCACAACGCGCCCGAAGGCCGGCTGAACTATCTCGAAC
GCGTGAACAGCGAGCATGAAAGCATCTACAGCGCCATCAGCAACCAGGATGCCGAGGCCGCCCGCGCCGCCATGCGGACC
CACCTGTCCAACAGCCGGGAGCGCCTGCGTCGCGGCAACAATCTGCTCTCCATGCAACCTCCGGACGGGCTCGCGAACCA
GGCCTGA

Upstream 100 bases:

>100_bases
TTTTCATGAAGTTTGTTAAAGTCAAACAAGTCGTCAGTCATAGGTTGTCTGATGACTGGGAATTCTCCAACCCCTCCATT
TGCTTGTCAACGGTTTTTTA

Downstream 100 bases:

>100_bases
CTGGCTCTGTGACACCTCTGTTGCGATGGCGTCGCATGGAGCCATGTTGTACGACAACTGACAATTAAGTGCAGCGTAGC
AACCCACCCGCCCGCCCCTC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTLRKTLSHPEPLPARHLAAPGARRPRGLVGEIVESLANSIRDGQLQPGAKLPTEAEIMARFDVSRTVVRESLSRLQASG
LVETRHGIGTFVLPPQESGNFRIMAEDFATVADVISVLELRISLETEAAGLAAQRRTPENLLAMQNALRAFRESINDDSD
AVPPDFQFHMEVARSTGNRHFADLMTYLGTMIIPRTRVNIVHNAPEGRLNYLERVNSEHESIYSAISNQDAEAARAAMRT
HLSNSRERLRRGNNLLSMQPPDGLANQA

Sequences:

>Translated_268_residues
MTLRKTLSHPEPLPARHLAAPGARRPRGLVGEIVESLANSIRDGQLQPGAKLPTEAEIMARFDVSRTVVRESLSRLQASG
LVETRHGIGTFVLPPQESGNFRIMAEDFATVADVISVLELRISLETEAAGLAAQRRTPENLLAMQNALRAFRESINDDSD
AVPPDFQFHMEVARSTGNRHFADLMTYLGTMIIPRTRVNIVHNAPEGRLNYLERVNSEHESIYSAISNQDAEAARAAMRT
HLSNSRERLRRGNNLLSMQPPDGLANQA
>Mature_267_residues
TLRKTLSHPEPLPARHLAAPGARRPRGLVGEIVESLANSIRDGQLQPGAKLPTEAEIMARFDVSRTVVRESLSRLQASGL
VETRHGIGTFVLPPQESGNFRIMAEDFATVADVISVLELRISLETEAAGLAAQRRTPENLLAMQNALRAFRESINDDSDA
VPPDFQFHMEVARSTGNRHFADLMTYLGTMIIPRTRVNIVHNAPEGRLNYLERVNSEHESIYSAISNQDAEAARAAMRTH
LSNSRERLRRGNNLLSMQPPDGLANQA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=241, Percent_Identity=32.3651452282158, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790032, Length=239, Percent_Identity=28.0334728033473, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082208, Length=231, Percent_Identity=29.4372294372294, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI48994955, Length=226, Percent_Identity=27.8761061946903, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786303, Length=242, Percent_Identity=30.5785123966942, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994961, Length=241, Percent_Identity=29.045643153527, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789621, Length=224, Percent_Identity=29.4642857142857, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29616; Mature: 29485

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRKTLSHPEPLPARHLAAPGARRPRGLVGEIVESLANSIRDGQLQPGAKLPTEAEIMA
CCCHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
RFDVSRTVVRESLSRLQASGLVETRHGIGTFVLPPQESGNFRIMAEDFATVADVISVLEL
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHH
RISLETEAAGLAAQRRTPENLLAMQNALRAFRESINDDSDAVPPDFQFHMEVARSTGNRH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHH
FADLMTYLGTMIIPRTRVNIVHNAPEGRLNYLERVNSEHESIYSAISNQDAEAARAAMRT
HHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHH
HLSNSRERLRRGNNLLSMQPPDGLANQA
HHHHHHHHHHHCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
TLRKTLSHPEPLPARHLAAPGARRPRGLVGEIVESLANSIRDGQLQPGAKLPTEAEIMA
CCHHHCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
RFDVSRTVVRESLSRLQASGLVETRHGIGTFVLPPQESGNFRIMAEDFATVADVISVLEL
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHH
RISLETEAAGLAAQRRTPENLLAMQNALRAFRESINDDSDAVPPDFQFHMEVARSTGNRH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHH
FADLMTYLGTMIIPRTRVNIVHNAPEGRLNYLERVNSEHESIYSAISNQDAEAARAAMRT
HHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHH
HLSNSRERLRRGNNLLSMQPPDGLANQA
HHHHHHHHHHHCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]