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The map label for this gene is rimO

Identifier: 91788563

GI number: 91788563

Start: 2845012

End: 2846421

Strand: Reverse

Name: rimO

Synonym: Bpro_2701

Alternate gene names: 91788563

Gene position: 2846421-2845012 (Counterclockwise)

Preceding gene: 91788564

Following gene: 91788562

Centisome position: 54.74

GC content: 61.63

Gene sequence:

>1410_bases
ATGAGCGAAGTACTTTCCCCGATGACAGCAATCTCCACCAAACCGGCCCCCCGTGTCGGGTTTGTCAGCCTGGGCTGCCC
CAAGGCGCTGACCGATTCCGAGTTGATACTCACACAATTGAGTGCTGAGGGCTATCAGACGTCCAAGACCTTTGAGGGCG
CAGACCTGGTGATCGTCAACACCTGCGGCTTCATTGACGACGCGGTCAAGGAAAGCCTGGACACCATCGGCGAGGCGCTG
GCAGAAAACGGCAGGGTCATCGTGACCGGCTGCCTGGGGGCCAAGGGCGGCGAGGGTGCTGGCAACCTGGTGCGCCAGAT
GCACCCCAGCGTGCTGGCTGTGACCGGCCCGCACGCCACGCAAGAGGTCATGGATGCGGTGCACCTGAACTTGCCCAAGC
CGCATGACCCGTTTGTGGACCTGGTGCCCAATGCGTTTGGCATTGCGGGCATCAAGCTCACACCGAAGCACTATGCCTAT
CTGAAGATCAGCGAAGGCTGCAACCATCGCTGCACCTTTTGCATCATCCCTTCCATGCGTGGCGACCTGGTGTCCCGGCC
CATCGGCGATGTGCTGAACGAAGCACGCGCGTTGTTTGAAGGGGGCGTGAAAGAACTGCTGGTGATCAGCCAGGACACCT
CCGCCTATGGCGTGGATGTGAAGTACCGCACCGGTTTCTGGGATGGCAAGCCGGTCAAGACCCGCATGCTGGAACTGGTC
CAGGCGCTGGGCGATATTGCCGAGCCCTATGGTGCCTGGGTTCGCCTGCACTATGTGTACCCTTACCCCAGCGTCGACGA
GGTGCTCCCGCTGATGGCCACCGGCAAGGTCCTGCCTTACCTGGATGTGCCTTTGCAGCACAGTCACCCTGATGTTTTGA
AGCGCATGAAACGCCCAGCCAGCGGCGAGAAAAATCTTGAGCGCATTGCACGCTGGCGCGAGATCTGTCCCGAAATTGTG
ATTCGCAGTACCTTTATTGCCGGCTTTCCGGGGGAAACCGAAGCTGAATTCGCGCACTTGCTGGAGTTCATGCGCGAGGC
CCGCATCGACCGTGCAGGCTGTTTTGCCTACAGTGCGGTGGAGGGCGCTACCGCCAATGACATTCCCGGCATGCTGCCGC
TGGAGGTGCGTGAAGAGCGGCGGGCGCGCTTCATGGCCGTGGCTGAGGAGGTTTCAAGCCTGAAGCTGCAGCAGCGCGTC
GGCGCCACGATGCAGGTGCTCGTCGATTCAGCGCCGGCCTTGGGCCGCAAGGGCGGCACGGGCCGCTCGTATGCGGACGC
GCCCGAGATTGATGGCGTGGTCAAACTGCTGCCGCCCGAGAAAATCAGCAAGACACTCAAGGTGGGTGAGTTCACCCGGG
CCCGTATTGTCGGAACCCAGGGCCACGACCTGGTGGCCATACCGGTCTGA

Upstream 100 bases:

>100_bases
GCCTGAAGCGCTGAACACTCAATTTTCTGCCTGATGTGCATGCAGGGCCATCGCGGCCCTGTCCGCTCCCGGCTCTGCCT
GCTCTGAGACAATAGAGGCC

Downstream 100 bases:

>100_bases
TTCATTTCCCGCTTCAGCGGGGCGGATTTTCAGCCGCCCCATGCGCACCAGCTTGCGTGCCCATATGGCGGACGAAAATA
GCGTATGAATTCGGATTCGG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSEVLSPMTAISTKPAPRVGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFIDDAVKESLDTIGEAL
AENGRVIVTGCLGAKGGEGAGNLVRQMHPSVLAVTGPHATQEVMDAVHLNLPKPHDPFVDLVPNAFGIAGIKLTPKHYAY
LKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLNEARALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELV
QALGDIAEPYGAWVRLHYVYPYPSVDEVLPLMATGKVLPYLDVPLQHSHPDVLKRMKRPASGEKNLERIARWREICPEIV
IRSTFIAGFPGETEAEFAHLLEFMREARIDRAGCFAYSAVEGATANDIPGMLPLEVREERRARFMAVAEEVSSLKLQQRV
GATMQVLVDSAPALGRKGGTGRSYADAPEIDGVVKLLPPEKISKTLKVGEFTRARIVGTQGHDLVAIPV

Sequences:

>Translated_469_residues
MSEVLSPMTAISTKPAPRVGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFIDDAVKESLDTIGEAL
AENGRVIVTGCLGAKGGEGAGNLVRQMHPSVLAVTGPHATQEVMDAVHLNLPKPHDPFVDLVPNAFGIAGIKLTPKHYAY
LKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLNEARALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELV
QALGDIAEPYGAWVRLHYVYPYPSVDEVLPLMATGKVLPYLDVPLQHSHPDVLKRMKRPASGEKNLERIARWREICPEIV
IRSTFIAGFPGETEAEFAHLLEFMREARIDRAGCFAYSAVEGATANDIPGMLPLEVREERRARFMAVAEEVSSLKLQQRV
GATMQVLVDSAPALGRKGGTGRSYADAPEIDGVVKLLPPEKISKTLKVGEFTRARIVGTQGHDLVAIPV
>Mature_468_residues
SEVLSPMTAISTKPAPRVGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVNTCGFIDDAVKESLDTIGEALA
ENGRVIVTGCLGAKGGEGAGNLVRQMHPSVLAVTGPHATQEVMDAVHLNLPKPHDPFVDLVPNAFGIAGIKLTPKHYAYL
KISEGCNHRCTFCIIPSMRGDLVSRPIGDVLNEARALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELVQ
ALGDIAEPYGAWVRLHYVYPYPSVDEVLPLMATGKVLPYLDVPLQHSHPDVLKRMKRPASGEKNLERIARWREICPEIVI
RSTFIAGFPGETEAEFAHLLEFMREARIDRAGCFAYSAVEGATANDIPGMLPLEVREERRARFMAVAEEVSSLKLQQRVG
ATMQVLVDSAPALGRKGGTGRSYADAPEIDGVVKLLPPEKISKTLKVGEFTRARIVGTQGHDLVAIPV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=456, Percent_Identity=24.5614035087719, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28872782, Length=456, Percent_Identity=24.5614035087719, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI93277076, Length=405, Percent_Identity=26.1728395061728, Blast_Score=126, Evalue=5e-29,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=59.2017738359202, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI1786882, Length=405, Percent_Identity=27.9012345679012, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=24.364406779661, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=28.6624203821656, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356207, Length=501, Percent_Identity=25.5489021956088, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922432, Length=358, Percent_Identity=27.6536312849162, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_POLSJ (Q12A25)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549515.1
- ProteinModelPortal:   Q12A25
- SMR:   Q12A25
- STRING:   Q12A25
- GeneID:   4014620
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2701
- NMPDR:   fig|296591.1.peg.419
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   HINDEML
- PhylomeDB:   Q12A25
- ProtClustDB:   PRK14862
- BioCyc:   PSP296591:BPRO_2701-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50880; Mature: 50749

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVLSPMTAISTKPAPRVGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVN
CCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEE
TCGFIDDAVKESLDTIGEALAENGRVIVTGCLGAKGGEGAGNLVRQMHPSVLAVTGPHAT
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCHH
QEVMDAVHLNLPKPHDPFVDLVPNAFGIAGIKLTPKHYAYLKISEGCNHRCTFCIIPSMR
HHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
GDLVSRPIGDVLNEARALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELV
CHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHH
QALGDIAEPYGAWVRLHYVYPYPSVDEVLPLMATGKVLPYLDVPLQHSHPDVLKRMKRPA
HHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCC
SGEKNLERIARWREICPEIVIRSTFIAGFPGETEAEFAHLLEFMREARIDRAGCFAYSAV
CCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCHHHHHEEHHC
EGATANDIPGMLPLEVREERRARFMAVAEEVSSLKLQQRVGATMQVLVDSAPALGRKGGT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCC
GRSYADAPEIDGVVKLLPPEKISKTLKVGEFTRARIVGTQGHDLVAIPV
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEECCCCCEEEECC
>Mature Secondary Structure 
SEVLSPMTAISTKPAPRVGFVSLGCPKALTDSELILTQLSAEGYQTSKTFEGADLVIVN
CHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEE
TCGFIDDAVKESLDTIGEALAENGRVIVTGCLGAKGGEGAGNLVRQMHPSVLAVTGPHAT
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCHH
QEVMDAVHLNLPKPHDPFVDLVPNAFGIAGIKLTPKHYAYLKISEGCNHRCTFCIIPSMR
HHHHHHHHCCCCCCCCCHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEECCCC
GDLVSRPIGDVLNEARALFEGGVKELLVISQDTSAYGVDVKYRTGFWDGKPVKTRMLELV
CHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHH
QALGDIAEPYGAWVRLHYVYPYPSVDEVLPLMATGKVLPYLDVPLQHSHPDVLKRMKRPA
HHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCC
SGEKNLERIARWREICPEIVIRSTFIAGFPGETEAEFAHLLEFMREARIDRAGCFAYSAV
CCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCCCHHHHHEEHHC
EGATANDIPGMLPLEVREERRARFMAVAEEVSSLKLQQRVGATMQVLVDSAPALGRKGGT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCC
GRSYADAPEIDGVVKLLPPEKISKTLKVGEFTRARIVGTQGHDLVAIPV
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA