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The map label for this gene is fixJ [H]

Identifier: 91788536

GI number: 91788536

Start: 2814240

End: 2814866

Strand: Direct

Name: fixJ [H]

Synonym: Bpro_2674

Alternate gene names: 91788536

Gene position: 2814240-2814866 (Clockwise)

Preceding gene: 91788535

Following gene: 91788537

Centisome position: 54.12

GC content: 60.45

Gene sequence:

>627_bases
ATGAGCTTGATTCCCAAAAAAGGCACTGTTTACGTCATTGATGATGACGAGGGCGTGCGCGATTCGCTGCAATGGCTGCT
CGAAGGCAAGGACTACCGGGTGCGCTGCTACGACTCGGCTGAAACATTTCTGAGCCGCTACGACGCGCGCGAAGTGGCCT
GCCTGATCGTGGATATCCGCATGGGCGGCATGACCGGCCTGGAGCTGCAGGACCGGCTGGTCGAACGCAAGTCGCCGTTG
CCGATTGTCTTCATCACCGGCCACGGCGATGTCCCCATGGCGGTCAACACCATGAAGAAAGGCGCCATGGACTTCATCCA
GAAGCCTTTTCAGGAAGAAGCGCTGGTCAATCTGGTCGAGCGCATGCTGGAACAGGCCAAGGAAGCCTTCGCAGGCCACC
AGCAATCCGCCAGCCGCGACGCCCTGCTGGCCAAGCTGACGTCGCGCGAAGCGCAGGTGCTCGAACGCATCGTGGCGGGC
CGCCTGAACAAGCAGATTGCCGACGACCTGGGCATCAGCATCAAGACGGTGGAAGCCCACCGCGCCAACATCATGGAAAA
GCTCAATGCCAATACCGTGGCTGACCTGCTGAAAATAGCGCTGGGCTCCAACGCGCCCAAGGCCTGA

Upstream 100 bases:

>100_bases
CTGCACCTTCTGCTAGCGATACTGTAGCGGCCAAGGATGCCGGCGTTCACTAAAGCACCTTCAGGAATAGTTTCGAGAAG
TTCACAAAAGAAAGTTTGGC

Downstream 100 bases:

>100_bases
GAGCCACCGGAGCCGAATTCCCAGCCGCTAACTTTTTATAGCTGCTTGCGCCCGTAGTTCCTGGACTAGCGGGCGTTTTT
ACTTAGAAAATTGAAATCAT

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSLIPKKGTVYVIDDDEGVRDSLQWLLEGKDYRVRCYDSAETFLSRYDAREVACLIVDIRMGGMTGLELQDRLVERKSPL
PIVFITGHGDVPMAVNTMKKGAMDFIQKPFQEEALVNLVERMLEQAKEAFAGHQQSASRDALLAKLTSREAQVLERIVAG
RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGSNAPKA

Sequences:

>Translated_208_residues
MSLIPKKGTVYVIDDDEGVRDSLQWLLEGKDYRVRCYDSAETFLSRYDAREVACLIVDIRMGGMTGLELQDRLVERKSPL
PIVFITGHGDVPMAVNTMKKGAMDFIQKPFQEEALVNLVERMLEQAKEAFAGHQQSASRDALLAKLTSREAQVLERIVAG
RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGSNAPKA
>Mature_207_residues
SLIPKKGTVYVIDDDEGVRDSLQWLLEGKDYRVRCYDSAETFLSRYDAREVACLIVDIRMGGMTGLELQDRLVERKSPLP
IVFITGHGDVPMAVNTMKKGAMDFIQKPFQEEALVNLVERMLEQAKEAFAGHQQSASRDALLAKLTSREAQVLERIVAGR
LNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGSNAPKA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786747, Length=184, Percent_Identity=26.6304347826087, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790102, Length=195, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=24.5283018867925, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788905, Length=162, Percent_Identity=27.1604938271605, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23084; Mature: 22953

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIPKKGTVYVIDDDEGVRDSLQWLLEGKDYRVRCYDSAETFLSRYDAREVACLIVDIR
CCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHCHHHEEEEEEEEE
MGGMTGLELQDRLVERKSPLPIVFITGHGDVPMAVNTMKKGAMDFIQKPFQEEALVNLVE
ECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RMLEQAKEAFAGHQQSASRDALLAKLTSREAQVLERIVAGRLNKQIADDLGISIKTVEAH
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHH
RANIMEKLNANTVADLLKIALGSNAPKA
HHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLIPKKGTVYVIDDDEGVRDSLQWLLEGKDYRVRCYDSAETFLSRYDAREVACLIVDIR
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHCHHHEEEEEEEEE
MGGMTGLELQDRLVERKSPLPIVFITGHGDVPMAVNTMKKGAMDFIQKPFQEEALVNLVE
ECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RMLEQAKEAFAGHQQSASRDALLAKLTSREAQVLERIVAGRLNKQIADDLGISIKTVEAH
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHH
RANIMEKLNANTVADLLKIALGSNAPKA
HHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]