Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 91788537

GI number: 91788537

Start: 2814965

End: 2815810

Strand: Direct

Name: folD [H]

Synonym: Bpro_2675

Alternate gene names: 91788537

Gene position: 2814965-2815810 (Clockwise)

Preceding gene: 91788536

Following gene: 91788558

Centisome position: 54.13

GC content: 62.88

Gene sequence:

>846_bases
ATGACTGCACAACTCATTGACGGGAATGCCCTCTCCAGACAACTGCGTGCGGAGGTCGCCCGGCGCGCGGCAGCGCTGCG
CGCCCGCGGCATCACGCCGGGTCTGGCGGTCGTGCTGGTGGGAGAAAATCCGGCCAGCCAGGTCTATGTGCGCAACAAGG
TCAAGGCCTGCCAGGACAACGGCCTGCATTCGGTGCTCGAGCACTACCCGGCCGCGCTCAGCGAGGCCGAGTTGCTGGCC
CGGGTCCATGCCCTGAACAATGACCCGGCCATCCACGGCATCCTGGTTCAGCTCCCACTGCCTGCGCATATAGACGCGCA
CAAGGTCATTGAAGCCATTGCCCCCGGCAAGGATGTCGACGGATTCCATGTGGCCAGCGCAGGCGCCCTGATGGTCGGAC
AGCCGGGCTTCTGGCCCTGCACACCCTATGGCTGCATGAAGATGCTGGAAAGCATTGGCTACGACCTGCGGGGCAAGCAC
GCCGTGGTCATTGGCCGCAGCAACATCGTGGGCAAGCCCATGGCCATGATGCTGCTGCAAAAAAATGCCACGGTGACGAT
CTGCCACAGCGCCACCAAAGACCTGAAGGCCATGACGCTGCAGGCCGACGTGATCGTAGCCGCAGTCGGCAAACGCAATG
TGCTGACCGCCGACATGGTCAAGCCGGGTGCTGTCGTCATTGACGTGGGCATGAACCGCAACGATGAAGGCAAACTCTGC
GGCGACGTCGACTTTGAAGGCGTCAAGGAGGTGGCAGGCTACATCACGCCGGTGCCTGGCGGCGTCGGCCCCATGACCAT
CACCATGCTGCTGGTCAACACGCTGGAATCTGCTGAGCGCCTTTAA

Upstream 100 bases:

>100_bases
GAGAGCCACCGGAGCCGAATTCCCAGCCGCTAACTTTTTATAGCTGCTTGCGCCCGTAGTTCCTGGACTAGCGGGCGTTT
TTACTTAGAAAATTGAAATC

Downstream 100 bases:

>100_bases
TCGGGACTGGATGCTGCCGACGCGGCGCCTGCCGCGGGTCGGCTCAGGCGCCAGAGGCTCAAGCCCGCGCACAACCACTG
GCCCAGGTACATGGCGCTTC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTAQLIDGNALSRQLRAEVARRAAALRARGITPGLAVVLVGENPASQVYVRNKVKACQDNGLHSVLEHYPAALSEAELLA
RVHALNNDPAIHGILVQLPLPAHIDAHKVIEAIAPGKDVDGFHVASAGALMVGQPGFWPCTPYGCMKMLESIGYDLRGKH
AVVIGRSNIVGKPMAMMLLQKNATVTICHSATKDLKAMTLQADVIVAAVGKRNVLTADMVKPGAVVIDVGMNRNDEGKLC
GDVDFEGVKEVAGYITPVPGGVGPMTITMLLVNTLESAERL

Sequences:

>Translated_281_residues
MTAQLIDGNALSRQLRAEVARRAAALRARGITPGLAVVLVGENPASQVYVRNKVKACQDNGLHSVLEHYPAALSEAELLA
RVHALNNDPAIHGILVQLPLPAHIDAHKVIEAIAPGKDVDGFHVASAGALMVGQPGFWPCTPYGCMKMLESIGYDLRGKH
AVVIGRSNIVGKPMAMMLLQKNATVTICHSATKDLKAMTLQADVIVAAVGKRNVLTADMVKPGAVVIDVGMNRNDEGKLC
GDVDFEGVKEVAGYITPVPGGVGPMTITMLLVNTLESAERL
>Mature_280_residues
TAQLIDGNALSRQLRAEVARRAAALRARGITPGLAVVLVGENPASQVYVRNKVKACQDNGLHSVLEHYPAALSEAELLAR
VHALNNDPAIHGILVQLPLPAHIDAHKVIEAIAPGKDVDGFHVASAGALMVGQPGFWPCTPYGCMKMLESIGYDLRGKHA
VVIGRSNIVGKPMAMMLLQKNATVTICHSATKDLKAMTLQADVIVAAVGKRNVLTADMVKPGAVVIDVGMNRNDEGKLCG
DVDFEGVKEVAGYITPVPGGVGPMTITMLLVNTLESAERL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=46.7796610169492, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=41.9243986254296, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=44.7457627118644, Blast_Score=227, Evalue=8e-60,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=25, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=52.158273381295, Blast_Score=269, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=42.8082191780822, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=45.993031358885, Blast_Score=265, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=40.6040268456376, Blast_Score=219, Evalue=3e-58,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=43.1034482758621, Blast_Score=250, Evalue=8e-67,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=43.1034482758621, Blast_Score=250, Evalue=8e-67,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=43.1034482758621, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=43.1034482758621, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.9387755102041, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=46.9387755102041, Blast_Score=248, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29700; Mature: 29569

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLIDGNALSRQLRAEVARRAAALRARGITPGLAVVLVGENPASQVYVRNKVKACQDN
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEEEHHHHHHHHCC
GLHSVLEHYPAALSEAELLARVHALNNDPAIHGILVQLPLPAHIDAHKVIEAIAPGKDVD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVASAGALMVGQPGFWPCTPYGCMKMLESIGYDLRGKHAVVIGRSNIVGKPMAMMLLQ
CEEEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHEEC
KNATVTICHSATKDLKAMTLQADVIVAAVGKRNVLTADMVKPGAVVIDVGMNRNDEGKLC
CCCEEEEEECCCCCHHHEEEECCEEEEECCCCCCEECCCCCCCEEEEEECCCCCCCCCEE
GDVDFEGVKEVAGYITPVPGGVGPMTITMLLVNTLESAERL
CCCCHHHHHHHHCEECCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAQLIDGNALSRQLRAEVARRAAALRARGITPGLAVVLVGENPASQVYVRNKVKACQDN
CCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEEEHHHHHHHHCC
GLHSVLEHYPAALSEAELLARVHALNNDPAIHGILVQLPLPAHIDAHKVIEAIAPGKDVD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVASAGALMVGQPGFWPCTPYGCMKMLESIGYDLRGKHAVVIGRSNIVGKPMAMMLLQ
CEEEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHEEC
KNATVTICHSATKDLKAMTLQADVIVAAVGKRNVLTADMVKPGAVVIDVGMNRNDEGKLC
CCCEEEEEECCCCCHHHEEEECCEEEEECCCCCCEECCCCCCCEEEEEECCCCCCCCCEE
GDVDFEGVKEVAGYITPVPGGVGPMTITMLLVNTLESAERL
CCCCHHHHHHHHCEECCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA