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The map label for this gene is ispG [H]

Identifier: 91788470

GI number: 91788470

Start: 2738568

End: 2739842

Strand: Reverse

Name: ispG [H]

Synonym: Bpro_2608

Alternate gene names: 91788470

Gene position: 2739842-2738568 (Counterclockwise)

Preceding gene: 91788471

Following gene: 91788469

Centisome position: 52.69

GC content: 62.75

Gene sequence:

>1275_bases
GTGGAGCCTTGCCTTCCCATCGAGTCGGCTTTTCCGAAAGCACGGCGTTCTACCCAGGCGCTGGTTGCCTGGGGCTCCCG
CGTGGTTACCGTGGGCGGCGACGCGCCGGTGCGTATCCAGTCCATGACCAACACGGACACCGTGGATGCCATTGGTACAG
CCATTCAGGTCAAGGAGCTTGCGCTGGCTGGCTCCGAAATGGTTCGCATCACCGTGAACACGCCTGAGGCGGCGCAGGCC
GTGCCTCACGTTCGGGAGCAGCTGGATCGCATGGGCATCGACGTGCCGCTGATTGGTGACTTTCATTACAACGGTCACCG
CCTGCTGACGGATTACCCGGCCTGTGCCGAGGCCTTGTCCAAATACCGCATCAACCCGGGTAACGTGGGCAAGGGCGACA
AGCGCGACCGGCAATTCGGCCAGATGATTGAAGTCGCCATGCGCTGGAACAAGCCCGTGCGCATCGGGGTGAACTGGGGC
AGCCTTGACCAGGAACTGCTGGCCAGCTTGATGGACGACAACAGTGCGCGTGCCACCCCGTGGGATGCCAGGCAGGTCAT
GTATGAGGCGCTGATCACCTCGGCCATTGATTCCGCCCGCCGTGCCGAGGAAATGGGCATGCGGCGTAACCAGATCATCC
TGTCCTGCAAGGTGAGCGGTGTCCAGGATCTCATTTCCGTGTACCGCGAATTGGCCAAACGCAGCGACTACGCCCTTCAC
CTCGGCCTCACCGAGGCCGGCATGGGCACCAAAGGCACGGTCGCCTCCGCCACCGCGCTGTCGATTTTGCTGCAGGAAGG
CATTGGCGACACGATTCGCGTCTCCCTGACCCCCCAGCCCGGCGAGGCCCGCACCCAGGAAGTGGTGATTGCTTCGGAGA
TCATCCAGGCCCTGGGGCTGCGCAACTTCGTCCCCAGCGTCACCGCCTGTCCCGGCTGTGGCCGCACCACCAGCACCACC
TTTCAGGAGCTTGCCAAGCAGATTGACGACTTCCTGCGGGCCCAGATGCCGGTCTGGCGCGCGCAGTACCCGGGCGTTGA
AGCGCTCAAGGTCGCGGTGATGGGCTGTATCGTCAATGGCCCCGGTGAAAGCAAGCATGCCGATATCGGCATCAGTCTGC
CTGGATCGGGCGAGGCGCCGGCCGCACCGGTATTTATTGATGGCGAGAAGAAGCTGACCCTGCGCGGAGACAACATTGCG
CAGGAGTTCCAGGTCATCGTTGAAAACTACATTGAAAAACGCTTTGGCACGCAGTCCGTCGCCGAAGCCGCGTGA

Upstream 100 bases:

>100_bases
TGGTTGGCCGGGCCGACGCCACCCAGGTGCAGGTGCGCGGCCGGGCATTCGACCTGACCGCCGTTGCCCGGGATAACGTG
GCCCGTTTCGAGGTGAAATA

Downstream 100 bases:

>100_bases
ATCCCATGCCTGAAAAAATGAATGCCGGCCAGACGGAGAAATTACCCTTGGCCAAAATCGAAAAACTCACCGCCGTCAAG
GGCATGAACGACATCTTGCC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEPCLPIESAFPKARRSTQALVAWGSRVVTVGGDAPVRIQSMTNTDTVDAIGTAIQVKELALAGSEMVRITVNTPEAAQA
VPHVREQLDRMGIDVPLIGDFHYNGHRLLTDYPACAEALSKYRINPGNVGKGDKRDRQFGQMIEVAMRWNKPVRIGVNWG
SLDQELLASLMDDNSARATPWDARQVMYEALITSAIDSARRAEEMGMRRNQIILSCKVSGVQDLISVYRELAKRSDYALH
LGLTEAGMGTKGTVASATALSILLQEGIGDTIRVSLTPQPGEARTQEVVIASEIIQALGLRNFVPSVTACPGCGRTTSTT
FQELAKQIDDFLRAQMPVWRAQYPGVEALKVAVMGCIVNGPGESKHADIGISLPGSGEAPAAPVFIDGEKKLTLRGDNIA
QEFQVIVENYIEKRFGTQSVAEAA

Sequences:

>Translated_424_residues
MEPCLPIESAFPKARRSTQALVAWGSRVVTVGGDAPVRIQSMTNTDTVDAIGTAIQVKELALAGSEMVRITVNTPEAAQA
VPHVREQLDRMGIDVPLIGDFHYNGHRLLTDYPACAEALSKYRINPGNVGKGDKRDRQFGQMIEVAMRWNKPVRIGVNWG
SLDQELLASLMDDNSARATPWDARQVMYEALITSAIDSARRAEEMGMRRNQIILSCKVSGVQDLISVYRELAKRSDYALH
LGLTEAGMGTKGTVASATALSILLQEGIGDTIRVSLTPQPGEARTQEVVIASEIIQALGLRNFVPSVTACPGCGRTTSTT
FQELAKQIDDFLRAQMPVWRAQYPGVEALKVAVMGCIVNGPGESKHADIGISLPGSGEAPAAPVFIDGEKKLTLRGDNIA
QEFQVIVENYIEKRFGTQSVAEAA
>Mature_424_residues
MEPCLPIESAFPKARRSTQALVAWGSRVVTVGGDAPVRIQSMTNTDTVDAIGTAIQVKELALAGSEMVRITVNTPEAAQA
VPHVREQLDRMGIDVPLIGDFHYNGHRLLTDYPACAEALSKYRINPGNVGKGDKRDRQFGQMIEVAMRWNKPVRIGVNWG
SLDQELLASLMDDNSARATPWDARQVMYEALITSAIDSARRAEEMGMRRNQIILSCKVSGVQDLISVYRELAKRSDYALH
LGLTEAGMGTKGTVASATALSILLQEGIGDTIRVSLTPQPGEARTQEVVIASEIIQALGLRNFVPSVTACPGCGRTTSTT
FQELAKQIDDFLRAQMPVWRAQYPGVEALKVAVMGCIVNGPGESKHADIGISLPGSGEAPAAPVFIDGEKKLTLRGDNIA
QEFQVIVENYIEKRFGTQSVAEAA

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=393, Percent_Identity=31.8066157760814, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45873; Mature: 45873

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPCLPIESAFPKARRSTQALVAWGSRVVTVGGDAPVRIQSMTNTDTVDAIGTAIQVKEL
CCCCCCCHHCCCHHHHHHHHHEECCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHH
ALAGSEMVRITVNTPEAAQAVPHVREQLDRMGIDVPLIGDFHYNGHRLLTDYPACAEALS
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECEEECCCEEEECCHHHHHHHH
KYRINPGNVGKGDKRDRQFGQMIEVAMRWNKPVRIGVNWGSLDQELLASLMDDNSARATP
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCC
WDARQVMYEALITSAIDSARRAEEMGMRRNQIILSCKVSGVQDLISVYRELAKRSDYALH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEE
LGLTEAGMGTKGTVASATALSILLQEGIGDTIRVSLTPQPGEARTQEVVIASEIIQALGL
EECHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RNFVPSVTACPGCGRTTSTTFQELAKQIDDFLRAQMPVWRAQYPGVEALKVAVMGCIVNG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHEECC
PGESKHADIGISLPGSGEAPAAPVFIDGEKKLTLRGDNIAQEFQVIVENYIEKRFGTQSV
CCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHH
AEAA
HHCC
>Mature Secondary Structure
MEPCLPIESAFPKARRSTQALVAWGSRVVTVGGDAPVRIQSMTNTDTVDAIGTAIQVKEL
CCCCCCCHHCCCHHHHHHHHHEECCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHH
ALAGSEMVRITVNTPEAAQAVPHVREQLDRMGIDVPLIGDFHYNGHRLLTDYPACAEALS
HHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECEEECCCEEEECCHHHHHHHH
KYRINPGNVGKGDKRDRQFGQMIEVAMRWNKPVRIGVNWGSLDQELLASLMDDNSARATP
HCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCC
WDARQVMYEALITSAIDSARRAEEMGMRRNQIILSCKVSGVQDLISVYRELAKRSDYALH
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEE
LGLTEAGMGTKGTVASATALSILLQEGIGDTIRVSLTPQPGEARTQEVVIASEIIQALGL
EECHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RNFVPSVTACPGCGRTTSTTFQELAKQIDDFLRAQMPVWRAQYPGVEALKVAVMGCIVNG
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHEECC
PGESKHADIGISLPGSGEAPAAPVFIDGEKKLTLRGDNIAQEFQVIVENYIEKRFGTQSV
CCCCCCCEEEEECCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHH
AEAA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]