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The map label for this gene is dppA [H]

Identifier: 91788395

GI number: 91788395

Start: 2648075

End: 2649682

Strand: Reverse

Name: dppA [H]

Synonym: Bpro_2532

Alternate gene names: 91788395

Gene position: 2649682-2648075 (Counterclockwise)

Preceding gene: 91788397

Following gene: 91788394

Centisome position: 50.95

GC content: 58.52

Gene sequence:

>1608_bases
ATGGAGACCTTTATGCGTCTGTCGTCAGCATCGAAATCGGGGAAAAATGGTTTGCTCGGGCTGGTGCTCGCCGCACTGTT
TGCGGTCACCGCGCCCATGGCCTCTGCGCAGGAATTCAAGCTGGCGATGAGCTCGCCGCCGACCTCGCTGGATCCGCATT
TCTACAACCTGTTTCCGAACCTGAATGTTTCCGATCATGTGTTTGAAACCCTGGTTACCATGGATGCCGACAGCCGGGTG
ATTCCCGGTCTGGCCGAGTCCTGGACGCTGGTCAATAACCTGACCTGGGAATTCAAGCTGCGCAAAGGCGTCAAGTTTCA
CGATGGCAGCGAGATGACCGCCGAAGACATTGCCTGGTCGATGGACAGGCCCTCGACCATCGTTGGCAGCCCCGGCAAGT
TCGATGTCTACACCAAGGCCATCATCAACAAGAAAATCATCGACCCCTACACCATCCGCTTCACCACCAAGGAGCCTTAC
CCGCTCATGCTGGCCGACTTGACCTCCGTTTACATCGTGTCCAAAAAGGCCACCACGGGCATTACGAGCGATGATTTTTC
CAGCGGCAAGGGCATGGTGGGTACCGGCCCCTTCAAGTTTGTCAGGTACCAGAGGGATGACCGGATCGAGCTTGATCGCA
ACGACAGTTACTGGGGCAAAAAGGCGGCCTGGTCCAAAGTGACCATGCGCTTCATTCCGAATGGTGCAACGCGCCTGGCG
GCGTTGCTGGCCGGCGATGTGCAGGCAATTGAAAACGTGCCAACGCCTGACCTGGCGAAGGTGCGTTCCGATGCCAGCAT
TGCGCTGTACTCCAAAGTCTCGCACCGCCTGATCTATCTGTACCCCGACACCAAGCGCGACAAGTCTCCGTTTGTGACCG
ACAAGGACGGCAAGGCCCTCGACAAGAACCCGCTGCAGGACCAGCGCGTGCGCGCCGCGCTGTCGATGGCGATCAACCGC
GAGGCGATTCGTGACCGCGTGATGGAAGGGCTGTCGGAGCCCACCAACAACCTGGTGCCCAAAACGCTGTTCGGCCACAA
CCCGGCCTTGAAGTCCGTCAAGTACGACCCGGACGGTGCCAAAAAGCTGCTGGCGACGGCCGGTTATCCGAACGGATTCG
GGCTGACGCTGCACACACCGAACAACCGCTATGTGAACGATGAAAAAATCGCACAGACCATTGCGCAGATGTTCTCGCGC
ATTGGCGTGGCCACCAAGGTTGAAGGCTTGCCGATGTCGGTGTATTCAGCCCGCGGTGCCAAAAGTGAATACTCCATGGG
TTTGCTGGGTTGGGGCGCACAAACCGGCGAAGTCTCCTCGCCGCTGCGTGCCCTGCTGGCCTGTGACAACCCGGCCAAAG
GCCTGGGCGGTTTCAACTGGCTCAAATACTGCAGCCCGAAGATGGATGAGCTGCTGCAAAAAGCCCTGAACACGGTTGAC
GACAAGGCGCGACTGGCGCTGCTGCAGGATGCCAGCGCCATTGCCATCAACGATGGCGGCCTCATTCCGATTCATCAGCA
GGTCACGACCTGGGCGACCAAAAAAGGAATTGCCTACATTCCGCGCACGGATGAGCGTACGCACGCCTACGGGTTCATCC
AGCAATAA

Upstream 100 bases:

>100_bases
TTGCTGCTGGGTTTTTGCGCAAAGGCAGGGCTTCTACCAGCAGATTTGGTATACAAGATGTGTATATTACGCCTACACCT
TCCAGGGTGTTTTTAACTTC

Downstream 100 bases:

>100_bases
ACGACTGACCACCATCCGCCCGCAAACACCGGGCGGATTTTTCAGCTTCCCGTAGCCCAGTTATTCAGGTTATTCGATGC
TCGTATTCATCATTCGGCGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
METFMRLSSASKSGKNGLLGLVLAALFAVTAPMASAQEFKLAMSSPPTSLDPHFYNLFPNLNVSDHVFETLVTMDADSRV
IPGLAESWTLVNNLTWEFKLRKGVKFHDGSEMTAEDIAWSMDRPSTIVGSPGKFDVYTKAIINKKIIDPYTIRFTTKEPY
PLMLADLTSVYIVSKKATTGITSDDFSSGKGMVGTGPFKFVRYQRDDRIELDRNDSYWGKKAAWSKVTMRFIPNGATRLA
ALLAGDVQAIENVPTPDLAKVRSDASIALYSKVSHRLIYLYPDTKRDKSPFVTDKDGKALDKNPLQDQRVRAALSMAINR
EAIRDRVMEGLSEPTNNLVPKTLFGHNPALKSVKYDPDGAKKLLATAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQMFSR
IGVATKVEGLPMSVYSARGAKSEYSMGLLGWGAQTGEVSSPLRALLACDNPAKGLGGFNWLKYCSPKMDELLQKALNTVD
DKARLALLQDASAIAINDGGLIPIHQQVTTWATKKGIAYIPRTDERTHAYGFIQQ

Sequences:

>Translated_535_residues
METFMRLSSASKSGKNGLLGLVLAALFAVTAPMASAQEFKLAMSSPPTSLDPHFYNLFPNLNVSDHVFETLVTMDADSRV
IPGLAESWTLVNNLTWEFKLRKGVKFHDGSEMTAEDIAWSMDRPSTIVGSPGKFDVYTKAIINKKIIDPYTIRFTTKEPY
PLMLADLTSVYIVSKKATTGITSDDFSSGKGMVGTGPFKFVRYQRDDRIELDRNDSYWGKKAAWSKVTMRFIPNGATRLA
ALLAGDVQAIENVPTPDLAKVRSDASIALYSKVSHRLIYLYPDTKRDKSPFVTDKDGKALDKNPLQDQRVRAALSMAINR
EAIRDRVMEGLSEPTNNLVPKTLFGHNPALKSVKYDPDGAKKLLATAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQMFSR
IGVATKVEGLPMSVYSARGAKSEYSMGLLGWGAQTGEVSSPLRALLACDNPAKGLGGFNWLKYCSPKMDELLQKALNTVD
DKARLALLQDASAIAINDGGLIPIHQQVTTWATKKGIAYIPRTDERTHAYGFIQQ
>Mature_535_residues
METFMRLSSASKSGKNGLLGLVLAALFAVTAPMASAQEFKLAMSSPPTSLDPHFYNLFPNLNVSDHVFETLVTMDADSRV
IPGLAESWTLVNNLTWEFKLRKGVKFHDGSEMTAEDIAWSMDRPSTIVGSPGKFDVYTKAIINKKIIDPYTIRFTTKEPY
PLMLADLTSVYIVSKKATTGITSDDFSSGKGMVGTGPFKFVRYQRDDRIELDRNDSYWGKKAAWSKVTMRFIPNGATRLA
ALLAGDVQAIENVPTPDLAKVRSDASIALYSKVSHRLIYLYPDTKRDKSPFVTDKDGKALDKNPLQDQRVRAALSMAINR
EAIRDRVMEGLSEPTNNLVPKTLFGHNPALKSVKYDPDGAKKLLATAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQMFSR
IGVATKVEGLPMSVYSARGAKSEYSMGLLGWGAQTGEVSSPLRALLACDNPAKGLGGFNWLKYCSPKMDELLQKALNTVD
DKARLALLQDASAIAINDGGLIPIHQQVTTWATKKGIAYIPRTDERTHAYGFIQQ

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=538, Percent_Identity=27.1375464684015, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1787052, Length=502, Percent_Identity=28.0876494023904, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI1787495, Length=529, Percent_Identity=24.952741020794, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI87081878, Length=500, Percent_Identity=25, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787551, Length=470, Percent_Identity=24.0425531914894, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1787762, Length=513, Percent_Identity=23.7816764132554, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789887, Length=529, Percent_Identity=25.3308128544423, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789397, Length=531, Percent_Identity=25.4237288135593, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58864; Mature: 58864

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFMRLSSASKSGKNGLLGLVLAALFAVTAPMASAQEFKLAMSSPPTSLDPHFYNLFPN
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHEEEECCCCCCCCCCHHEECCC
LNVSDHVFETLVTMDADSRVIPGLAESWTLVNNLTWEFKLRKGVKFHDGSEMTAEDIAWS
CCCHHHHHHHHHHCCCCCCCCCCCHHCEEEHHCCEEEEEECCCCEECCCCCCCHHHHHCC
MDRPSTIVGSPGKFDVYTKAIINKKIIDPYTIRFTTKEPYPLMLADLTSVYIVSKKATTG
CCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCEEEEEECCCCCC
ITSDDFSSGKGMVGTGPFKFVRYQRDDRIELDRNDSYWGKKAAWSKVTMRFIPNGATRLA
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHEEEEECCCCHHHHH
ALLAGDVQAIENVPTPDLAKVRSDASIALYSKVSHRLIYLYPDTKRDKSPFVTDKDGKAL
HHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCEECCCCCCC
DKNPLQDQRVRAALSMAINREAIRDRVMEGLSEPTNNLVPKTLFGHNPALKSVKYDPDGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHCCEEECCCHH
KKLLATAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQMFSRIGVATKVEGLPMSVYSARGA
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCC
KSEYSMGLLGWGAQTGEVSSPLRALLACDNPAKGLGGFNWLKYCSPKMDELLQKALNTVD
CCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCC
DKARLALLQDASAIAINDGGLIPIHQQVTTWATKKGIAYIPRTDERTHAYGFIQQ
HHHHEEEEECCCEEEECCCCEEEEHHHHHHHHHCCCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
METFMRLSSASKSGKNGLLGLVLAALFAVTAPMASAQEFKLAMSSPPTSLDPHFYNLFPN
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHEEEECCCCCCCCCCHHEECCC
LNVSDHVFETLVTMDADSRVIPGLAESWTLVNNLTWEFKLRKGVKFHDGSEMTAEDIAWS
CCCHHHHHHHHHHCCCCCCCCCCCHHCEEEHHCCEEEEEECCCCEECCCCCCCHHHHHCC
MDRPSTIVGSPGKFDVYTKAIINKKIIDPYTIRFTTKEPYPLMLADLTSVYIVSKKATTG
CCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCEEEEEECCCCCC
ITSDDFSSGKGMVGTGPFKFVRYQRDDRIELDRNDSYWGKKAAWSKVTMRFIPNGATRLA
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCHHHHHHEEEEECCCCHHHHH
ALLAGDVQAIENVPTPDLAKVRSDASIALYSKVSHRLIYLYPDTKRDKSPFVTDKDGKAL
HHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCEECCCCCCC
DKNPLQDQRVRAALSMAINREAIRDRVMEGLSEPTNNLVPKTLFGHNPALKSVKYDPDGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCHHCCEEECCCHH
KKLLATAGYPNGFGLTLHTPNNRYVNDEKIAQTIAQMFSRIGVATKVEGLPMSVYSARGA
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHCCCC
KSEYSMGLLGWGAQTGEVSSPLRALLACDNPAKGLGGFNWLKYCSPKMDELLQKALNTVD
CCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCC
DKARLALLQDASAIAINDGGLIPIHQQVTTWATKKGIAYIPRTDERTHAYGFIQQ
HHHHEEEEECCCEEEECCCCEEEEHHHHHHHHHCCCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]