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The map label for this gene is ogt [H]

Identifier: 91787519

GI number: 91787519

Start: 1691675

End: 1692199

Strand: Reverse

Name: ogt [H]

Synonym: Bpro_1637

Alternate gene names: 91787519

Gene position: 1692199-1691675 (Counterclockwise)

Preceding gene: 91787520

Following gene: 91787518

Centisome position: 32.54

GC content: 64.57

Gene sequence:

>525_bases
ATGAAATTTGACCGCTCCATCGTTCAGACCACGTTCCAAAGCCCTCTGGGCAAAATGATCATCGCCGCAACGGACAAGGG
CCTGGCCGGCACCTGGTTTGCCGGCCAACGCCACCTGCCGTCACAGCTGGTGGAGCCTTATGTGTGGCCCGAAGACGCCA
ACCACCCGGTGCTGAAACAGGCCATTGCACAGCTCACCGAGTACTTCGCCGGAACGCGCACGGTGTTTGATCTGCCCCTG
GACCTGGACTACGGCACGCCCTTTCAGCAAACGGTGTGGCAGGCCCTGCTGGCCATACCGCAGGGCGGCACCACCAGCTA
TGGCGAGCTCAGCAGCCGCATCGGCAAACCGGCGGCCGTGCGCGCCGTGGGCGCGGCGATCGGGCGCAACCCGGTCAGCG
TCATCGTGCCCTGCCACCGCGTGCTGGGCGCCGACGGCTCGCTCACCGGCTATGCGGGCGGCCTCGACAAAAAAACAGCA
CTGCTCAAGCTTGAGGGTGCGCTCATGAAGGAGACCCTGACTTGA

Upstream 100 bases:

>100_bases
GAATGATCGAGCCAGCCTTTTTCTGACCTTTTCTGAATACGCCTGCATGACAGGTAAACCCTCGAAACTCGAAACTCGAA
ACCATTCAAAGGTGAACAAG

Downstream 100 bases:

>100_bases
CCTCATCCACCACCGGGCTGGCATCATCCGCGGCATCATCCGCGGCACCATCCGCGGCACCATCCGCGGCATCACCCGCG
ACCCTGGCTGCACCCAAAAG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKFDRSIVQTTFQSPLGKMIIAATDKGLAGTWFAGQRHLPSQLVEPYVWPEDANHPVLKQAIAQLTEYFAGTRTVFDLPL
DLDYGTPFQQTVWQALLAIPQGGTTSYGELSSRIGKPAAVRAVGAAIGRNPVSVIVPCHRVLGADGSLTGYAGGLDKKTA
LLKLEGALMKETLT

Sequences:

>Translated_174_residues
MKFDRSIVQTTFQSPLGKMIIAATDKGLAGTWFAGQRHLPSQLVEPYVWPEDANHPVLKQAIAQLTEYFAGTRTVFDLPL
DLDYGTPFQQTVWQALLAIPQGGTTSYGELSSRIGKPAAVRAVGAAIGRNPVSVIVPCHRVLGADGSLTGYAGGLDKKTA
LLKLEGALMKETLT
>Mature_174_residues
MKFDRSIVQTTFQSPLGKMIIAATDKGLAGTWFAGQRHLPSQLVEPYVWPEDANHPVLKQAIAQLTEYFAGTRTVFDLPL
DLDYGTPFQQTVWQALLAIPQGGTTSYGELSSRIGKPAAVRAVGAAIGRNPVSVIVPCHRVLGADGSLTGYAGGLDKKTA
LLKLEGALMKETLT

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=173, Percent_Identity=32.9479768786127, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=106, Percent_Identity=50.9433962264151, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788542, Length=155, Percent_Identity=38.7096774193548, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=48.2352941176471, Blast_Score=89, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=48.2352941176471, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=43.1818181818182, Blast_Score=92, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137554, Length=84, Percent_Identity=41.6666666666667, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18653; Mature: 18653

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDRSIVQTTFQSPLGKMIIAATDKGLAGTWFAGQRHLPSQLVEPYVWPEDANHPVLKQ
CCCCHHHHHHHHHCCHHCEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHH
AIAQLTEYFAGTRTVFDLPLDLDYGTPFQQTVWQALLAIPQGGTTSYGELSSRIGKPAAV
HHHHHHHHHCCCCEEEECCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RAVGAAIGRNPVSVIVPCHRVLGADGSLTGYAGGLDKKTALLKLEGALMKETLT
HHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFDRSIVQTTFQSPLGKMIIAATDKGLAGTWFAGQRHLPSQLVEPYVWPEDANHPVLKQ
CCCCHHHHHHHHHCCHHCEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHH
AIAQLTEYFAGTRTVFDLPLDLDYGTPFQQTVWQALLAIPQGGTTSYGELSSRIGKPAAV
HHHHHHHHHCCCCEEEECCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHH
RAVGAAIGRNPVSVIVPCHRVLGADGSLTGYAGGLDKKTALLKLEGALMKETLT
HHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]