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The map label for this gene is ada [H]

Identifier: 91787520

GI number: 91787520

Start: 1692391

End: 1693905

Strand: Reverse

Name: ada [H]

Synonym: Bpro_1638

Alternate gene names: 91787520

Gene position: 1693905-1692391 (Counterclockwise)

Preceding gene: 91787533

Following gene: 91787519

Centisome position: 32.57

GC content: 67.85

Gene sequence:

>1515_bases
ATGACCAGCGCCCAGCCCCCCCTCGCCGACAACGACTGCTACCTCGCCCTGAAGGCGCGGGACGCACGCTTTGACGGCAG
TTTTTTTACCGGTGTTACATCCACCGGCATCTACTGCCGGCCGGTCTGCAGCGTCAAAACACCCAAGCGCGAAAACTGCC
GGTTTTTCAGCCATGCGGCACAGGCCGAAAGCGCCGGCTTTCGTCCCTGCCTGCGCTGCCGGCCCGAACTGGCGCCGCAC
TCGGTGGTGTGGTCCATCCAGGACGCGTCCTACATCCTGGCCCACCAGGCGGCCCGCCTGCTGGACGAGCCTGAGCTGTC
CGACGAAGCCCCTTCGGTCGAAAAACTGGCGGCGCGGCTGGGCGTGAGCGACCGCCACGTGCGGCGGATTTTCGAGGCGC
AGTTTGGCGTGTCGCCCATGCAGTACCTGCAGACGCGCCGGCTGCACACCGCCAAGCAGTTGCTGGCCGATACCAGCCTG
CCCATCACGCAGGTTGCGCTGATCAGCGGCTTTGCCAGCGTGCGGCGCTTCAATGCCGCCTTTGTCGAGCACTACAAGCT
AAACCCCACGCAGCTGCGGCGCCAGGGGGTGAGCGATGCCGGCCACATCAACGGCACCGCGCCGGGCATCACCATCCGGC
TGGGCTACCGGCCCCCTTACGACGTGGCGGCCATGCTCGGATTTTTTAGCAAACGCACCATCAGCGCTATTGAATTCGTG
GCTGCCGATGCGCAACACCCCTCGATAGGCAGGACATTTCGTGTGGAGTCGGGCGGCAAGGTACATGCCGGCTGGCTGCT
GGCGGCCTTTGATGAAACCCGTTCCCGGCTGGTGCTCAACGTCAGCGACTCGCTGCGCGAGGTGCTGCCGCTGGTGATTC
GCCGGGTGCGCGCCACGTTTGACCTCGACGCCGACCCTGCGGCCATCAACAGCGTGCTGCATGCCGGCTTTCCGCAGGGC
GATGGCCTGCGCGTGCCCGGTGCGCTCGATGGCTATGAGCTGGCCGTGCGCGCGGTGCTGGGCCAGCAGATCACCGTGGC
CGCGGCGCGCACGCTGGCGCAGCGCATGGTCGACCGCTTTGGCGAGCCGGTGCAAACCCCCTGGCCGCAGCTGACCCGGC
TGTTCCCGGCGCCTGCCATGCTGGCCGCCGCCAGCGGCGATGCGCTGGGGCAGCTCGGCATAGTCAGGCAGCGCCAGGCC
GCCATTGTGGGCATTGCGCAAGCGGTGGCCGACAAACGCCTGCAGCTGCACAGCGGCGCCGATGTGCATGCCACGCTGGA
GGCGCTGAAGGCCTTGCCCGGCATCGGCGACTGGACCGCGCAGTACATCGCCATGCGCGCCCTGCGCTGGCCCGATGCGT
TTCCGGCGGGCGATGTGGCGCTGCACAAGGCGATGGGCGTGCAGGGACTGAAAAATCCGGCCCGGGAAGCCGAGTTGGCC
TCGCATGCGTGGAAGCCCTGGCGCAGCTATGCCGTGATCCGCGCGTGGAGCGGCACGCTGGAGCGGCCCGGTTGA

Upstream 100 bases:

>100_bases
ATTGTAGGGTGGCGCCCTCCCTCGGGACGGGCCCCGGCGGGCTTGAGGGATATGTCCGAAAACGGCTAGTCAAAAGCTGC
GCCCGGCGTAACATGCCCCC

Downstream 100 bases:

>100_bases
CGGGGGCTGAGGAGATTCATGCGCCTAGGGAATGAATACGCCCTCTCGATTTCATTGGACGGGAAAGCGCCTTCGGGCGA
CCATCACCGGCGAATGATCG

Product: transcriptional regulator Ada/DNA-O6-methylguanine--protein-cysteine S-methyltransferase/DNA-3-methyladenine glycosylase II

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MTSAQPPLADNDCYLALKARDARFDGSFFTGVTSTGIYCRPVCSVKTPKRENCRFFSHAAQAESAGFRPCLRCRPELAPH
SVVWSIQDASYILAHQAARLLDEPELSDEAPSVEKLAARLGVSDRHVRRIFEAQFGVSPMQYLQTRRLHTAKQLLADTSL
PITQVALISGFASVRRFNAAFVEHYKLNPTQLRRQGVSDAGHINGTAPGITIRLGYRPPYDVAAMLGFFSKRTISAIEFV
AADAQHPSIGRTFRVESGGKVHAGWLLAAFDETRSRLVLNVSDSLREVLPLVIRRVRATFDLDADPAAINSVLHAGFPQG
DGLRVPGALDGYELAVRAVLGQQITVAAARTLAQRMVDRFGEPVQTPWPQLTRLFPAPAMLAAASGDALGQLGIVRQRQA
AIVGIAQAVADKRLQLHSGADVHATLEALKALPGIGDWTAQYIAMRALRWPDAFPAGDVALHKAMGVQGLKNPAREAELA
SHAWKPWRSYAVIRAWSGTLERPG

Sequences:

>Translated_504_residues
MTSAQPPLADNDCYLALKARDARFDGSFFTGVTSTGIYCRPVCSVKTPKRENCRFFSHAAQAESAGFRPCLRCRPELAPH
SVVWSIQDASYILAHQAARLLDEPELSDEAPSVEKLAARLGVSDRHVRRIFEAQFGVSPMQYLQTRRLHTAKQLLADTSL
PITQVALISGFASVRRFNAAFVEHYKLNPTQLRRQGVSDAGHINGTAPGITIRLGYRPPYDVAAMLGFFSKRTISAIEFV
AADAQHPSIGRTFRVESGGKVHAGWLLAAFDETRSRLVLNVSDSLREVLPLVIRRVRATFDLDADPAAINSVLHAGFPQG
DGLRVPGALDGYELAVRAVLGQQITVAAARTLAQRMVDRFGEPVQTPWPQLTRLFPAPAMLAAASGDALGQLGIVRQRQA
AIVGIAQAVADKRLQLHSGADVHATLEALKALPGIGDWTAQYIAMRALRWPDAFPAGDVALHKAMGVQGLKNPAREAELA
SHAWKPWRSYAVIRAWSGTLERPG
>Mature_503_residues
TSAQPPLADNDCYLALKARDARFDGSFFTGVTSTGIYCRPVCSVKTPKRENCRFFSHAAQAESAGFRPCLRCRPELAPHS
VVWSIQDASYILAHQAARLLDEPELSDEAPSVEKLAARLGVSDRHVRRIFEAQFGVSPMQYLQTRRLHTAKQLLADTSLP
ITQVALISGFASVRRFNAAFVEHYKLNPTQLRRQGVSDAGHINGTAPGITIRLGYRPPYDVAAMLGFFSKRTISAIEFVA
ADAQHPSIGRTFRVESGGKVHAGWLLAAFDETRSRLVLNVSDSLREVLPLVIRRVRATFDLDADPAAINSVLHAGFPQGD
GLRVPGALDGYELAVRAVLGQQITVAAARTLAQRMVDRFGEPVQTPWPQLTRLFPAPAMLAAASGDALGQLGIVRQRQAA
IVGIAQAVADKRLQLHSGADVHATLEALKALPGIGDWTAQYIAMRALRWPDAFPAGDVALHKAMGVQGLKNPAREAELAS
HAWKPWRSYAVIRAWSGTLERPG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=283, Percent_Identity=34.2756183745583, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1788542, Length=190, Percent_Identity=32.6315789473684, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 54880; Mature: 54749

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAQPPLADNDCYLALKARDARFDGSFFTGVTSTGIYCRPVCSVKTPKRENCRFFSHAA
CCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
QAESAGFRPCLRCRPELAPHSVVWSIQDASYILAHQAARLLDEPELSDEAPSVEKLAARL
HHHCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
GVSDRHVRRIFEAQFGVSPMQYLQTRRLHTAKQLLADTSLPITQVALISGFASVRRFNAA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FVEHYKLNPTQLRRQGVSDAGHINGTAPGITIRLGYRPPYDVAAMLGFFSKRTISAIEFV
HHHHHCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AADAQHPSIGRTFRVESGGKVHAGWLLAAFDETRSRLVLNVSDSLREVLPLVIRRVRATF
HCCCCCCCCCCEEEECCCCEEECCCEEEEEHHCCCEEEEECHHHHHHHHHHHHHHHHHHC
DLDADPAAINSVLHAGFPQGDGLRVPGALDGYELAVRAVLGQQITVAAARTLAQRMVDRF
CCCCCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GEPVQTPWPQLTRLFPAPAMLAAASGDALGQLGIVRQRQAAIVGIAQAVADKRLQLHSGA
CCCCCCCCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVHATLEALKALPGIGDWTAQYIAMRALRWPDAFPAGDVALHKAMGVQGLKNPAREAELA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHCCCHHHHHHHH
SHAWKPWRSYAVIRAWSGTLERPG
HHCCCHHHHEEEEEECCCCCCCCC
>Mature Secondary Structure 
TSAQPPLADNDCYLALKARDARFDGSFFTGVTSTGIYCRPVCSVKTPKRENCRFFSHAA
CCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHH
QAESAGFRPCLRCRPELAPHSVVWSIQDASYILAHQAARLLDEPELSDEAPSVEKLAARL
HHHCCCCCHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
GVSDRHVRRIFEAQFGVSPMQYLQTRRLHTAKQLLADTSLPITQVALISGFASVRRFNAA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
FVEHYKLNPTQLRRQGVSDAGHINGTAPGITIRLGYRPPYDVAAMLGFFSKRTISAIEFV
HHHHHCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AADAQHPSIGRTFRVESGGKVHAGWLLAAFDETRSRLVLNVSDSLREVLPLVIRRVRATF
HCCCCCCCCCCEEEECCCCEEECCCEEEEEHHCCCEEEEECHHHHHHHHHHHHHHHHHHC
DLDADPAAINSVLHAGFPQGDGLRVPGALDGYELAVRAVLGQQITVAAARTLAQRMVDRF
CCCCCHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GEPVQTPWPQLTRLFPAPAMLAAASGDALGQLGIVRQRQAAIVGIAQAVADKRLQLHSGA
CCCCCCCCHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVHATLEALKALPGIGDWTAQYIAMRALRWPDAFPAGDVALHKAMGVQGLKNPAREAELA
CHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHCCCHHHHHHHH
SHAWKPWRSYAVIRAWSGTLERPG
HHCCCHHHHEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]