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The map label for this gene is purF [H]

Identifier: 91787491

GI number: 91787491

Start: 1658252

End: 1659769

Strand: Direct

Name: purF [H]

Synonym: Bpro_1607

Alternate gene names: 91787491

Gene position: 1658252-1659769 (Clockwise)

Preceding gene: 91787490

Following gene: 91787492

Centisome position: 31.89

GC content: 62.25

Gene sequence:

>1518_bases
ATGTGTGGAATAGTCGGCGTCGTCAGCAAAGCCCCGGTCAACCAGCTGATCTATGACGGCCTGCTGCTGCTGCAGCACCG
CGGCCAGGATGCGGCAGGCATCGTCACGCAGCAGGGGCGCAAGTTTTACATGCACAAGGCCAAGGGCATGGTGCGCGACG
TGTTTCGCACCCGCAACATGCGCGCGTTGCCGGGCAATGTGGGCCTGGGCCAGGTGCGTTATCCCACGGCCGGCAATGCC
TACAGCGAAGACGAGGCCCAGCCGTTTTACGTCAATGCGCCGTTCGGGCTGGTGCTCTCGCACAACGGCAACCTGACGAA
TGCGGCCGAACTCAAGGCCGAGCTGTTCAACACCGACCACCGGCACATCAACACCGAGAGCGACTCTGAAGTGCTGCTCA
ACGTGCTGGCGCATGAGCTGGAAAAAACCACGCGCGGCCTGCCGCTCAATCCGGTTGATGTGTTCGCGGCGGTGCGCGGC
GTGCACAAACGGGTGAAAGGCTCTTACGCCGTGGTGGCGCTGATTGCCGGCCACGGCCTGCTGGCTTTCCGCGATCCGTT
CGGCATTCGCCCGCTGTGCATAGGCCACGGCCAGAACGAGGATGGCGGCACCGTGATGGTGGCCAGCGAGTCGGTGGCGC
TGGAAGGCACCTCGCACCAGTTTGACCGCGACATTGCGCCGGGCGAAGCGGTGTTCGTGGATATGGAAGGCACCGTGCAT
GCCGAGCAATGCGCGGCTTCACCCAAGCTCAATCCCTGCATTTTCGAGTTTGTCTACCTGGCCCGGCCCGACTCGGTGCT
GGACGGCATTTCGGTCTACCAGGCGCGGCTGAACCTGGGTGAAACGCTGGCCAAGCGCGTGGTCTCCACCGTGCCGCCCA
GCGAGATTGACGTCATCATCCCGATTCCCGAATCCAGCCGCCCCAGCGCGACACAATTGGCACACCTGCTGGGCATTCCG
TATCGCGAAGGTTTTGTGAAGAACCGCTACGTCGGCCGCACCTTCATCATGCCGGGGCAGGGCGTGCGCAAAAAATCAGT
GCGCCAGAAGCTCAATGTGATTGCGAGTGAATTCAAGGGCCGCAATGTGCTGCTGGTGGACGACTCCATCGTGCGCGGCA
CCACCAGCCGCGAAATCGTGCAGATGGCGCGTGACGCCGGCGCGCGCAAGGTGTATCTGGCCAGCGCGGCGCCGCCCGTG
CGTTATCCCAACGTCTACGGCATTGACATGCCCACGGCGACGGAACTGGTGGCGCATGACCGCACCGTTGAGGAAATCCG
CCAGGTGATTGGCTGCGATGCCCTGATTTACCAGGACGTGGAGAGCATGAAACGCGCCGTGGGCTCGCTCAATCCCAAGC
TGGACGGTTTTGATGCATCGTGCTTTGACGGCGTTTACATCACCGGGGACGTGACCAGCGAGACGATTGAAAAGATGAAC
CAACAACGCGTGGATACCAGTGAAGAAGGCGATGTGGATGTGTCGCGCCTGGCGCTTCCCAATCCGCAAGAGGCCTGA

Upstream 100 bases:

>100_bases
ACCCCCACGCTTTTCACTTCGTGTGAGGTGCGGCCCCCAAAACGGGCGCATTTTCCCCGGGGCGGTCCAGCGGAAAATTT
ATGAATGGAAATATAAGCTT

Downstream 100 bases:

>100_bases
ACGATGAGCAACAAGAAACTGCCTGAGAACCTGCACCTCGACACGCTGGCCGTGCGCGCCGCCGTCGACAAAAGCCAGTA
CGGCGAAAACTCCGAAGCGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MCGIVGVVSKAPVNQLIYDGLLLLQHRGQDAAGIVTQQGRKFYMHKAKGMVRDVFRTRNMRALPGNVGLGQVRYPTAGNA
YSEDEAQPFYVNAPFGLVLSHNGNLTNAAELKAELFNTDHRHINTESDSEVLLNVLAHELEKTTRGLPLNPVDVFAAVRG
VHKRVKGSYAVVALIAGHGLLAFRDPFGIRPLCIGHGQNEDGGTVMVASESVALEGTSHQFDRDIAPGEAVFVDMEGTVH
AEQCAASPKLNPCIFEFVYLARPDSVLDGISVYQARLNLGETLAKRVVSTVPPSEIDVIIPIPESSRPSATQLAHLLGIP
YREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPV
RYPNVYGIDMPTATELVAHDRTVEEIRQVIGCDALIYQDVESMKRAVGSLNPKLDGFDASCFDGVYITGDVTSETIEKMN
QQRVDTSEEGDVDVSRLALPNPQEA

Sequences:

>Translated_505_residues
MCGIVGVVSKAPVNQLIYDGLLLLQHRGQDAAGIVTQQGRKFYMHKAKGMVRDVFRTRNMRALPGNVGLGQVRYPTAGNA
YSEDEAQPFYVNAPFGLVLSHNGNLTNAAELKAELFNTDHRHINTESDSEVLLNVLAHELEKTTRGLPLNPVDVFAAVRG
VHKRVKGSYAVVALIAGHGLLAFRDPFGIRPLCIGHGQNEDGGTVMVASESVALEGTSHQFDRDIAPGEAVFVDMEGTVH
AEQCAASPKLNPCIFEFVYLARPDSVLDGISVYQARLNLGETLAKRVVSTVPPSEIDVIIPIPESSRPSATQLAHLLGIP
YREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPV
RYPNVYGIDMPTATELVAHDRTVEEIRQVIGCDALIYQDVESMKRAVGSLNPKLDGFDASCFDGVYITGDVTSETIEKMN
QQRVDTSEEGDVDVSRLALPNPQEA
>Mature_505_residues
MCGIVGVVSKAPVNQLIYDGLLLLQHRGQDAAGIVTQQGRKFYMHKAKGMVRDVFRTRNMRALPGNVGLGQVRYPTAGNA
YSEDEAQPFYVNAPFGLVLSHNGNLTNAAELKAELFNTDHRHINTESDSEVLLNVLAHELEKTTRGLPLNPVDVFAAVRG
VHKRVKGSYAVVALIAGHGLLAFRDPFGIRPLCIGHGQNEDGGTVMVASESVALEGTSHQFDRDIAPGEAVFVDMEGTVH
AEQCAASPKLNPCIFEFVYLARPDSVLDGISVYQARLNLGETLAKRVVSTVPPSEIDVIIPIPESSRPSATQLAHLLGIP
YREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPV
RYPNVYGIDMPTATELVAHDRTVEEIRQVIGCDALIYQDVESMKRAVGSLNPKLDGFDASCFDGVYITGDVTSETIEKMN
QQRVDTSEEGDVDVSRLALPNPQEA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=35.3684210526316, Blast_Score=272, Evalue=7e-73,
Organism=Escherichia coli, GI1788651, Length=490, Percent_Identity=56.530612244898, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1790167, Length=225, Percent_Identity=29.3333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=487, Percent_Identity=33.8809034907598, Blast_Score=249, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6323958, Length=491, Percent_Identity=50.10183299389, Blast_Score=472, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=34.9494949494949, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=34.8088531187123, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24659604, Length=475, Percent_Identity=35.7894736842105, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55015; Mature: 55015

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVVSKAPVNQLIYDGLLLLQHRGQDAAGIVTQQGRKFYMHKAKGMVRDVFRTRNM
CCCCCCCCCCCCHHHHHHHHHHEEHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCC
RALPGNVGLGQVRYPTAGNAYSEDEAQPFYVNAPFGLVLSHNGNLTNAAELKAELFNTDH
EECCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCHHHHHHHHHHCCCC
RHINTESDSEVLLNVLAHELEKTTRGLPLNPVDVFAAVRGVHKRVKGSYAVVALIAGHGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCE
LAFRDPFGIRPLCIGHGQNEDGGTVMVASESVALEGTSHQFDRDIAPGEAVFVDMEGTVH
EEECCCCCCCCEEEECCCCCCCCEEEEEECCEEECCCCCHHCCCCCCCCEEEEECCCCEE
AEQCAASPKLNPCIFEFVYLARPDSVLDGISVYQARLNLGETLAKRVVSTVPPSEIDVII
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPESSRPSATQLAHLLGIPYREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKG
ECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
RNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYPNVYGIDMPTATELVAHD
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
RTVEEIRQVIGCDALIYQDVESMKRAVGSLNPKLDGFDASCFDGVYITGDVTSETIEKMN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEECCCCHHHHHHHH
QQRVDTSEEGDVDVSRLALPNPQEA
HHHCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MCGIVGVVSKAPVNQLIYDGLLLLQHRGQDAAGIVTQQGRKFYMHKAKGMVRDVFRTRNM
CCCCCCCCCCCCHHHHHHHHHHEEHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCC
RALPGNVGLGQVRYPTAGNAYSEDEAQPFYVNAPFGLVLSHNGNLTNAAELKAELFNTDH
EECCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEECCCCCCHHHHHHHHHHCCCC
RHINTESDSEVLLNVLAHELEKTTRGLPLNPVDVFAAVRGVHKRVKGSYAVVALIAGHGL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCE
LAFRDPFGIRPLCIGHGQNEDGGTVMVASESVALEGTSHQFDRDIAPGEAVFVDMEGTVH
EEECCCCCCCCEEEECCCCCCCCEEEEEECCEEECCCCCHHCCCCCCCCEEEEECCCCEE
AEQCAASPKLNPCIFEFVYLARPDSVLDGISVYQARLNLGETLAKRVVSTVPPSEIDVII
HHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
PIPESSRPSATQLAHLLGIPYREGFVKNRYVGRTFIMPGQGVRKKSVRQKLNVIASEFKG
ECCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCC
RNVLLVDDSIVRGTTSREIVQMARDAGARKVYLASAAPPVRYPNVYGIDMPTATELVAHD
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHCC
RTVEEIRQVIGCDALIYQDVESMKRAVGSLNPKLDGFDASCFDGVYITGDVTSETIEKMN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCEEEECCCCHHHHHHHH
QQRVDTSEEGDVDVSRLALPNPQEA
HHHCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]