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The map label for this gene is metZ [H]

Identifier: 91787492

GI number: 91787492

Start: 1659773

End: 1661008

Strand: Direct

Name: metZ [H]

Synonym: Bpro_1608

Alternate gene names: 91787492

Gene position: 1659773-1661008 (Clockwise)

Preceding gene: 91787491

Following gene: 91787493

Centisome position: 31.92

GC content: 62.94

Gene sequence:

>1236_bases
ATGAGCAACAAGAAACTGCCTGAGAACCTGCACCTCGACACGCTGGCCGTGCGCGCCGCCGTCGACAAAAGCCAGTACGG
CGAAAACTCCGAAGCGCTGTACCTGACCAGCAGCTTTGTGCAGCCCGATGCCGCCACTGCGGCGCGGCGCTTTGCCAATG
AGGAAGAGGGCTACATCTATTCGCGCTTTACCAACCCCACGGTGACCAGCATGGAGCAGCGCCTCGCGGCGATGGAGGGA
ACCGAAGCCTGCATCGGCACCTCCAGCGGCATGGCGGCCATCCTGCTGATGTGCATGGGCCTGCTCAAAAGCGGCGACCA
CGTGATCTGCTCGCAGTCGGTGTTTGGCTCCACCATCAAACTGATTGGCAGCGAGTTCGGCAAGTTCGGTGTCGAAAGCA
CTTTTGTGTCGCAAACCGACGTGGCCGAGTGGAGGGCGGCGATCAAGCCCAACACCAGGCTGCTGTTTGCCGAAACGCCG
ACCAATCCGCTGACCGAGATATGCGACATACGCGCGCTGGCCGACATCGCGCATGCCGCAGGCGCCCTGCTGGCCGTGGA
CAACTGCTTTTGCTCACCCGCGCTGCAGCAGCCCGTGAAACTGGGCGCCGACCTGATCATCCATTCAGGCACCAAATACC
TCGACGGTCAGGGCCGTGTGATCGCCGGTGCGATTTGCGGCACCAGGCAACTCATTGACGACAAGCTGGTCCCCATCATG
CGCAGCGCCGGCATGACGCTGTCGGCGTTCAACGCCTGGGTGGTGCTCAAGGGCCTGGAAACACTGTCCATTCGCATGCA
GGCGCAAAGCGCCAATGCGCTGGCGCTGGCCACCTGGCTGGAGGCGCAACCGGGCGTGGCCCGCGTGTATTACCCGGGCC
TCGAGTCGCACCCGCAATATGCGTTGGCGATGCGGCAGCAAACCATCAACGGGCAGGGCTGCGGCGGCGCGGTACTGTCG
TTTGATGTCAATGGCAGCGATGTCGAAAGCGCCCGCAAAAATGCCTTCCACGTGATCGACAGCACGCAAGTCTGCAGCAT
CACGGCCAACCTGGGCGACACCAAGACCACGATCACCCATCCGGCCACCACTTCGCACGGCCGGCTCAGTGAAGCGCAGC
GGCAGGCCGCCGGCATAGGCCAGGGCCTGATCCGCGTGGCCGCGGGCCTGGAACACATTGACGATTTGAAAGCCGATCTC
CGCAGAGGTCTGGACGGTCTGGGCCGTTTGAAATGA

Upstream 100 bases:

>100_bases
GACGATTGAAAAGATGAACCAACAACGCGTGGATACCAGTGAAGAAGGCGATGTGGATGTGTCGCGCCTGGCGCTTCCCA
ATCCGCAAGAGGCCTGAACG

Downstream 100 bases:

>100_bases
CACAGAAAATACGAACGCGTTTTGCGCCATCGCCCACGGGCTTTATCCACCTGGGCAACATCCGCTCGGCGCTGTACCCG
TGGGCATTTGCCCGCGCCAC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSNKKLPENLHLDTLAVRAAVDKSQYGENSEALYLTSSFVQPDAATAARRFANEEEGYIYSRFTNPTVTSMEQRLAAMEG
TEACIGTSSGMAAILLMCMGLLKSGDHVICSQSVFGSTIKLIGSEFGKFGVESTFVSQTDVAEWRAAIKPNTRLLFAETP
TNPLTEICDIRALADIAHAAGALLAVDNCFCSPALQQPVKLGADLIIHSGTKYLDGQGRVIAGAICGTRQLIDDKLVPIM
RSAGMTLSAFNAWVVLKGLETLSIRMQAQSANALALATWLEAQPGVARVYYPGLESHPQYALAMRQQTINGQGCGGAVLS
FDVNGSDVESARKNAFHVIDSTQVCSITANLGDTKTTITHPATTSHGRLSEAQRQAAGIGQGLIRVAAGLEHIDDLKADL
RRGLDGLGRLK

Sequences:

>Translated_411_residues
MSNKKLPENLHLDTLAVRAAVDKSQYGENSEALYLTSSFVQPDAATAARRFANEEEGYIYSRFTNPTVTSMEQRLAAMEG
TEACIGTSSGMAAILLMCMGLLKSGDHVICSQSVFGSTIKLIGSEFGKFGVESTFVSQTDVAEWRAAIKPNTRLLFAETP
TNPLTEICDIRALADIAHAAGALLAVDNCFCSPALQQPVKLGADLIIHSGTKYLDGQGRVIAGAICGTRQLIDDKLVPIM
RSAGMTLSAFNAWVVLKGLETLSIRMQAQSANALALATWLEAQPGVARVYYPGLESHPQYALAMRQQTINGQGCGGAVLS
FDVNGSDVESARKNAFHVIDSTQVCSITANLGDTKTTITHPATTSHGRLSEAQRQAAGIGQGLIRVAAGLEHIDDLKADL
RRGLDGLGRLK
>Mature_410_residues
SNKKLPENLHLDTLAVRAAVDKSQYGENSEALYLTSSFVQPDAATAARRFANEEEGYIYSRFTNPTVTSMEQRLAAMEGT
EACIGTSSGMAAILLMCMGLLKSGDHVICSQSVFGSTIKLIGSEFGKFGVESTFVSQTDVAEWRAAIKPNTRLLFAETPT
NPLTEICDIRALADIAHAAGALLAVDNCFCSPALQQPVKLGADLIIHSGTKYLDGQGRVIAGAICGTRQLIDDKLVPIMR
SAGMTLSAFNAWVVLKGLETLSIRMQAQSANALALATWLEAQPGVARVYYPGLESHPQYALAMRQQTINGQGCGGAVLSF
DVNGSDVESARKNAFHVIDSTQVCSITANLGDTKTTITHPATTSHGRLSEAQRQAAGIGQGLIRVAAGLEHIDDLKADLR
RGLDGLGRLK

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=388, Percent_Identity=33.2474226804124, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI299473759, Length=387, Percent_Identity=29.7157622739018, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI25453487, Length=386, Percent_Identity=27.4611398963731, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1790375, Length=394, Percent_Identity=34.5177664974619, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1789383, Length=366, Percent_Identity=26.2295081967213, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560056, Length=386, Percent_Identity=34.1968911917098, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=386, Percent_Identity=34.4559585492228, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=412, Percent_Identity=31.0679611650485, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=439, Percent_Identity=31.6628701594533, Blast_Score=215, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=404, Percent_Identity=31.6831683168317, Blast_Score=209, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=27.536231884058, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=280, Percent_Identity=27.5, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=360, Percent_Identity=26.9444444444444, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922554, Length=354, Percent_Identity=35.5932203389831, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43788; Mature: 43657

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKLPENLHLDTLAVRAAVDKSQYGENSEALYLTSSFVQPDAATAARRFANEEEGYIY
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEE
SRFTNPTVTSMEQRLAAMEGTEACIGTSSGMAAILLMCMGLLKSGDHVICSQSVFGSTIK
EEECCCHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
LIGSEFGKFGVESTFVSQTDVAEWRAAIKPNTRLLFAETPTNPLTEICDIRALADIAHAA
HHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
GALLAVDNCFCSPALQQPVKLGADLIIHSGTKYLDGQGRVIAGAICGTRQLIDDKLVPIM
HHHEEEHHHHCCHHHHHHHHCCCEEEEECCCCEECCCCCEEEEEHHCHHHHHHHHHHHHH
RSAGMTLSAFNAWVVLKGLETLSIRMQAQSANALALATWLEAQPGVARVYYPGLESHPQY
HHCCCEEEHHHHEEEEECCEEEEEEEEECCCCEEEEEEHHCCCCCEEEEEECCCCCCCHH
ALAMRQQTINGQGCGGAVLSFDVNGSDVESARKNAFHVIDSTQVCSITANLGDTKTTITH
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCEEEECCCEEEEEEECCCCCCEEEEC
PATTSHGRLSEAQRQAAGIGQGLIRVAAGLEHIDDLKADLRRGLDGLGRLK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCC
>Mature Secondary Structure 
SNKKLPENLHLDTLAVRAAVDKSQYGENSEALYLTSSFVQPDAATAARRFANEEEGYIY
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEE
SRFTNPTVTSMEQRLAAMEGTEACIGTSSGMAAILLMCMGLLKSGDHVICSQSVFGSTIK
EEECCCHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHH
LIGSEFGKFGVESTFVSQTDVAEWRAAIKPNTRLLFAETPTNPLTEICDIRALADIAHAA
HHHHHHHHCCCCHHHCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
GALLAVDNCFCSPALQQPVKLGADLIIHSGTKYLDGQGRVIAGAICGTRQLIDDKLVPIM
HHHEEEHHHHCCHHHHHHHHCCCEEEEECCCCEECCCCCEEEEEHHCHHHHHHHHHHHHH
RSAGMTLSAFNAWVVLKGLETLSIRMQAQSANALALATWLEAQPGVARVYYPGLESHPQY
HHCCCEEEHHHHEEEEECCEEEEEEEEECCCCEEEEEEHHCCCCCEEEEEECCCCCCCHH
ALAMRQQTINGQGCGGAVLSFDVNGSDVESARKNAFHVIDSTQVCSITANLGDTKTTITH
HHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHCCEEEECCCEEEEEEECCCCCCEEEEC
PATTSHGRLSEAQRQAAGIGQGLIRVAAGLEHIDDLKADLRRGLDGLGRLK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]