BacMap

Definition	Polaromonas sp. JS666 chromosome, complete genome.
Accession	NC_007948
Length	5,200,264

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The map label for this gene is bioF2

Identifier: 91787464

GI number: 91787464

Start: 1627675

End: 1628892

Strand: Direct

Name: bioF2

Synonym: Bpro_1579

Alternate gene names: 91787464

Gene position: 1627675-1628892 (Clockwise)

Preceding gene: 91787463

Following gene: 91787465

Centisome position: 31.3

GC content: 68.97

Gene sequence:

>1218_bases
ATGCTGATCGACCACTTGAACCGTCAACAGCTTGAGCGCGAGGCCCAAGGGCTACAGCGCCAGCGCCGGATCGCCGAGTC
CCCGTGCGCGCCGCGCCAGTGGGTCAGCCAGGACGGCCAGCCGGCGCGCGAAATGCTGGCGTTTTGCAGCAACGACTACC
TGGGCCTGGCCAATCACCCGGCCCTGGTCGAGGCGCTGGCGGAAGGTGCGCGGCAATTTGGCGCGGGCAGCGGCGCCTCG
CACCTGATCAGCGGTCACTCACGCGCCCATGCCGCGCTGGAGGGCGACTTGGCCGCCTGGCTGGCACCCAGCATTCCCAA
TGCCGCGGCGCTGTACTTCTGCACCGGCTACCTGGCCAACCTGGCCTTGCTCACCGCGCTGGGCGACGCCAGCGCCACGA
TCTTCGCCGACAAGCTCAACCATGCGTCGCTGATTGACGGTGCGCTGCTGGCCAAGGCGACTATGCAGCGCTATGCGCAC
CGCAATCTGACGGTGCTGGCCAGCCAGCTCGAAAGTTGCACGACGCCCATCAAGCTGATCGTGACCGATGCGGTCTTCAG
CATGGACGGCGATCTGGCCGACCTGCCGGCGCTGCTGGCGCTGGCGGAGCGTTTTGACGCCTGGCTGATCGTGGATGACG
CCCATGGCTTTGGCGTGCTCGGTGATCAGGGGCGGGGCAGCCTGTCGCATTTTGGCCTGCGCAGCGAACGGCTGATCTAC
ATGGGCACCCTGGGCAAAGCGGCGGGCCTGGGCGGCGCTTTTGTCGCGGCCCACCCGAGCATCATCGACTGGCTGGTGCA
GGCCGCCCGCCCCTACATCTACACCACCGCCGCCCCACCCGCCGTGGCCCATGCCTTGCGCGAAAGCCTGCGCCTCATCA
GCAGCGCCGAGGGCGAGCAGCGGCGCGCGCATCTGCAGCAACTGATCATCCAGCTGCGCACGCAGCTATCCGCCCTGATC
GGCGCGCACCCGACCCTGGGTTGGCGGCTGGCGGACTCCAGCACCGCGATTCAGCCACTGATCGTGGGCGACAACGCCGC
CGCCCTGGCCCTGATGGCGGCACTCGATGCGCAAGGTCTGTGGGTGCCGGCCATCCGTCCGCCCACGGTGCCGGTGGGCA
CGGCCCGGCTGCGCATCACGCTCAGCGCCACTCATAGCGCCGCGGATGTGCAGCGCCTGGTCGATGGCCTGGCCTGCGCA
GCCCGGGAGTTGCCATGA

Upstream 100 bases:

>100_bases
CATGTGATGCCGCACCCCGGCGGCCTACCCGCCACCACCCTGGCCCAGCGCGTGCCGGCAACGCTGGACGATGTGCTGAC
CCAAACCGCAGGCTTCTCCG

Downstream 100 bases:

>100_bases
CAACACTGCCTCCGTTACACGGCGGTTTCGTCACTCGCCGCGTGGCGGCCCATCTGGACAATACGGCCCTCAGAAGCGTG
TTTTCATGGCTATCCCTTTT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS 2; 7-keto-8-amino-pelargonic acid synthase 2; 7-KAP synthase 2; KAPA synthase 2; 8-amino-7-ketopelargonate synthase 2; L-alanine--pimeloyl-CoA ligase 2

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MLIDHLNRQQLEREAQGLQRQRRIAESPCAPRQWVSQDGQPAREMLAFCSNDYLGLANHPALVEALAEGARQFGAGSGAS
HLISGHSRAHAALEGDLAAWLAPSIPNAAALYFCTGYLANLALLTALGDASATIFADKLNHASLIDGALLAKATMQRYAH
RNLTVLASQLESCTTPIKLIVTDAVFSMDGDLADLPALLALAERFDAWLIVDDAHGFGVLGDQGRGSLSHFGLRSERLIY
MGTLGKAAGLGGAFVAAHPSIIDWLVQAARPYIYTTAAPPAVAHALRESLRLISSAEGEQRRAHLQQLIIQLRTQLSALI
GAHPTLGWRLADSSTAIQPLIVGDNAAALALMAALDAQGLWVPAIRPPTVPVGTARLRITLSATHSAADVQRLVDGLACA
ARELP

Sequences:

>Translated_405_residues
MLIDHLNRQQLEREAQGLQRQRRIAESPCAPRQWVSQDGQPAREMLAFCSNDYLGLANHPALVEALAEGARQFGAGSGAS
HLISGHSRAHAALEGDLAAWLAPSIPNAAALYFCTGYLANLALLTALGDASATIFADKLNHASLIDGALLAKATMQRYAH
RNLTVLASQLESCTTPIKLIVTDAVFSMDGDLADLPALLALAERFDAWLIVDDAHGFGVLGDQGRGSLSHFGLRSERLIY
MGTLGKAAGLGGAFVAAHPSIIDWLVQAARPYIYTTAAPPAVAHALRESLRLISSAEGEQRRAHLQQLIIQLRTQLSALI
GAHPTLGWRLADSSTAIQPLIVGDNAAALALMAALDAQGLWVPAIRPPTVPVGTARLRITLSATHSAADVQRLVDGLACA
ARELP
>Mature_405_residues
MLIDHLNRQQLEREAQGLQRQRRIAESPCAPRQWVSQDGQPAREMLAFCSNDYLGLANHPALVEALAEGARQFGAGSGAS
HLISGHSRAHAALEGDLAAWLAPSIPNAAALYFCTGYLANLALLTALGDASATIFADKLNHASLIDGALLAKATMQRYAH
RNLTVLASQLESCTTPIKLIVTDAVFSMDGDLADLPALLALAERFDAWLIVDDAHGFGVLGDQGRGSLSHFGLRSERLIY
MGTLGKAAGLGGAFVAAHPSIIDWLVQAARPYIYTTAAPPAVAHALRESLRLISSAEGEQRRAHLQQLIIQLRTQLSALI
GAHPTLGWRLADSSTAIQPLIVGDNAAALALMAALDAQGLWVPAIRPPTVPVGTARLRITLSATHSAADVQRLVDGLACA
ARELP

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=403, Percent_Identity=32.258064516129, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI284448556, Length=361, Percent_Identity=33.2409972299169, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI83977442, Length=370, Percent_Identity=32.1621621621622, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI83977440, Length=370, Percent_Identity=32.1621621621622, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI83977444, Length=370, Percent_Identity=32.1621621621622, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4502025, Length=360, Percent_Identity=32.2222222222222, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI40316939, Length=360, Percent_Identity=32.2222222222222, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4758668, Length=362, Percent_Identity=28.4530386740331, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI119220554, Length=376, Percent_Identity=25.531914893617, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI5454084, Length=251, Percent_Identity=22.3107569721116, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786993, Length=355, Percent_Identity=47.3239436619718, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1790046, Length=394, Percent_Identity=35.0253807106599, Blast_Score=218, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI71994529, Length=367, Percent_Identity=31.6076294277929, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI32566772, Length=422, Percent_Identity=23.9336492890995, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560914, Length=381, Percent_Identity=23.0971128608924, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560912, Length=381, Percent_Identity=23.0971128608924, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71982625, Length=397, Percent_Identity=20.4030226700252, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982617, Length=397, Percent_Identity=20.4030226700252, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320438, Length=433, Percent_Identity=30.2540415704388, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6320267, Length=363, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6323954, Length=261, Percent_Identity=26.4367816091954, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24662918, Length=365, Percent_Identity=34.2465753424658, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI17137420, Length=359, Percent_Identity=30.08356545961, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI17136286, Length=361, Percent_Identity=26.0387811634349, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653280, Length=276, Percent_Identity=23.1884057971014, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653276, Length=276, Percent_Identity=23.1884057971014, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653278, Length=276, Percent_Identity=23.1884057971014, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF2_POLSJ (Q12D74)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548416.1
- ProteinModelPortal:   Q12D74
- STRING:   Q12D74
- GeneID:   4015781
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1579
- NMPDR:   fig|296591.1.peg.173
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   ATVMAMS
- PhylomeDB:   Q12D74
- ProtClustDB:   PRK05958
- BioCyc:   PSP296591:BPRO_1579-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 42935; Mature: 42935

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 20-20 BINDING 141-141 BINDING 187-187 BINDING 369-369

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDHLNRQQLEREAQGLQRQRRIAESPCAPRQWVSQDGQPAREMLAFCSNDYLGLANHP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCH
ALVEALAEGARQFGAGSGASHLISGHSRAHAALEGDLAAWLAPSIPNAAALYFCTGYLAN
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHH
LALLTALGDASATIFADKLNHASLIDGALLAKATMQRYAHRNLTVLASQLESCTTPIKLI
HHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHE
VTDAVFSMDGDLADLPALLALAERFDAWLIVDDAHGFGVLGDQGRGSLSHFGLRSERLIY
EHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEECCCCCCCHHHCCCCCCCEEE
MGTLGKAAGLGGAFVAAHPSIIDWLVQAARPYIYTTAAPPAVAHALRESLRLISSAEGEQ
EECCCHHCCCCCHHHHCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHH
RRAHLQQLIIQLRTQLSALIGAHPTLGWRLADSSTAIQPLIVGDNAAALALMAALDAQGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCC
WVPAIRPPTVPVGTARLRITLSATHSAADVQRLVDGLACAARELP
EECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIDHLNRQQLEREAQGLQRQRRIAESPCAPRQWVSQDGQPAREMLAFCSNDYLGLANHP
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCH
ALVEALAEGARQFGAGSGASHLISGHSRAHAALEGDLAAWLAPSIPNAAALYFCTGYLAN
HHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHH
LALLTALGDASATIFADKLNHASLIDGALLAKATMQRYAHRNLTVLASQLESCTTPIKLI
HHHHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHE
VTDAVFSMDGDLADLPALLALAERFDAWLIVDDAHGFGVLGDQGRGSLSHFGLRSERLIY
EHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEECCCCCCCHHHCCCCCCCEEE
MGTLGKAAGLGGAFVAAHPSIIDWLVQAARPYIYTTAAPPAVAHALRESLRLISSAEGEQ
EECCCHHCCCCCHHHHCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHH
RRAHLQQLIIQLRTQLSALIGAHPTLGWRLADSSTAIQPLIVGDNAAALALMAALDAQGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCC
WVPAIRPPTVPVGTARLRITLSATHSAADVQRLVDGLACAARELP
EECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA