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The map label for this gene is bioB2 [H]

Identifier: 91787465

GI number: 91787465

Start: 1629025

End: 1630059

Strand: Direct

Name: bioB2 [H]

Synonym: Bpro_1580

Alternate gene names: 91787465

Gene position: 1629025-1630059 (Clockwise)

Preceding gene: 91787464

Following gene: 91787466

Centisome position: 31.33

GC content: 66.38

Gene sequence:

>1035_bases
ATGATGAGCATTGCCACGATTCCCGTTTCGTCCCTGCGTCGCGCGCCCGCGCCCACCACCGCGCCGCAGGCACCGCTTCG
CTGGCAGGTCGAAGACATAGCCGCCCTGTACGAACTGCCGTTCATGGACTTGCTGTTTCGCGCCCAGCAGGTGCACCGCG
AACACTTTGACGCCAACGAAGTGCAGCTGTCCACACTGCTGTCCATCAAGACCGGCGGCTGCGCCGAAGACTGCGGCTAC
TGCCCGCAGTCGACCCATTTCGAGACCGAGGTGAAAGCCAGCAAGCTGATGCCACTGGCCGAGGTCATCGAGGCAGCCCG
GGCCGCCAAAGACCAGGGCGCCACCCGCTTTTGCATGGGCGCGGCCTGGCGCAGCCCGAAAGAGCGCGACATGGAGCGCG
TGACCGAGATAGTGCGCGAGGTCCGCTCGCTGGGCCTGGAGACCTGCATGACCTTGGGCATGCTGCAGGCCGAGCAGGCC
CAGGCCCTGAAGGACGCCGGGCTGGACTACTACAACCACAACCTGGACAGCGCGCCCGAGTTTTATGGCGACATCATCAG
TACCCGCACCTACCAGGACCGGCTCGACACCCTGGGCCATGTGCGCCAGGCCGGCATCAACGTCTGCTGCGGTGGCATCG
TCGGCATGGGCGAAAGCCGGCTGCAGCGCGCCGGGCTGATTGCGCAGCTGGCCAACCTGAGCCCCTACCCGGAGTCGGTG
CCCATCAACAACCTGGTCCCGGTGGCAGGCACGCCGCTGGCCAACACCGCGCCGCTGGACCCGTTCGAGTTCGTGCGCAC
CATCGCCGTGGCCCGCATCACCATGCCGCTGACCATGGTCCGCCTGTCGGCCGGTCGCGAGCAGATGGATGAAGCCCTGC
AGGCGCTGTGCTTCGCGGCGGGCGCCAATTCGATTTTTTATGGCGACAAGCTGCTCACGACCAGCAACCCGCAAGCCGAT
CGCGACCGCCAGCTGTTTGAGCGCCTGGGACTAAAAACCCAGGGCGCTCGCCCCGCAGCGCAACAGGCGCAGTAG

Upstream 100 bases:

>100_bases
ACTCGCCGCGTGGCGGCCCATCTGGACAATACGGCCCTCAGAAGCGTGTTTTCATGGCTATCCCTTTTTTCTCTTCCCTC
TTCACCTGAGAAATGCACTC

Downstream 100 bases:

>100_bases
ACATGAACGCAGACCTGAGGCCGCCAGCGCCGGCGTACCCCATCATTGATGCCGCGTTCCTGGCCAACCCTTACCCGACT
TACGACGCCCTGCGGGAGGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MMSIATIPVSSLRRAPAPTTAPQAPLRWQVEDIAALYELPFMDLLFRAQQVHREHFDANEVQLSTLLSIKTGGCAEDCGY
CPQSTHFETEVKASKLMPLAEVIEAARAAKDQGATRFCMGAAWRSPKERDMERVTEIVREVRSLGLETCMTLGMLQAEQA
QALKDAGLDYYNHNLDSAPEFYGDIISTRTYQDRLDTLGHVRQAGINVCCGGIVGMGESRLQRAGLIAQLANLSPYPESV
PINNLVPVAGTPLANTAPLDPFEFVRTIAVARITMPLTMVRLSAGREQMDEALQALCFAAGANSIFYGDKLLTTSNPQAD
RDRQLFERLGLKTQGARPAAQQAQ

Sequences:

>Translated_344_residues
MMSIATIPVSSLRRAPAPTTAPQAPLRWQVEDIAALYELPFMDLLFRAQQVHREHFDANEVQLSTLLSIKTGGCAEDCGY
CPQSTHFETEVKASKLMPLAEVIEAARAAKDQGATRFCMGAAWRSPKERDMERVTEIVREVRSLGLETCMTLGMLQAEQA
QALKDAGLDYYNHNLDSAPEFYGDIISTRTYQDRLDTLGHVRQAGINVCCGGIVGMGESRLQRAGLIAQLANLSPYPESV
PINNLVPVAGTPLANTAPLDPFEFVRTIAVARITMPLTMVRLSAGREQMDEALQALCFAAGANSIFYGDKLLTTSNPQAD
RDRQLFERLGLKTQGARPAAQQAQ
>Mature_344_residues
MMSIATIPVSSLRRAPAPTTAPQAPLRWQVEDIAALYELPFMDLLFRAQQVHREHFDANEVQLSTLLSIKTGGCAEDCGY
CPQSTHFETEVKASKLMPLAEVIEAARAAKDQGATRFCMGAAWRSPKERDMERVTEIVREVRSLGLETCMTLGMLQAEQA
QALKDAGLDYYNHNLDSAPEFYGDIISTRTYQDRLDTLGHVRQAGINVCCGGIVGMGESRLQRAGLIAQLANLSPYPESV
PINNLVPVAGTPLANTAPLDPFEFVRTIAVARITMPLTMVRLSAGREQMDEALQALCFAAGANSIFYGDKLLTTSNPQAD
RDRQLFERLGLKTQGARPAAQQAQ

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=63.961038961039, Blast_Score=423, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6321725, Length=313, Percent_Identity=48.2428115015974, Blast_Score=317, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37673; Mature: 37673

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSIATIPVSSLRRAPAPTTAPQAPLRWQVEDIAALYELPFMDLLFRAQQVHREHFDANE
CCCEECCCHHHHHCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VQLSTLLSIKTGGCAEDCGYCPQSTHFETEVKASKLMPLAEVIEAARAAKDQGATRFCMG
HHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
AAWRSPKERDMERVTEIVREVRSLGLETCMTLGMLQAEQAQALKDAGLDYYNHNLDSAPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHH
FYGDIISTRTYQDRLDTLGHVRQAGINVCCGGIVGMGESRLQRAGLIAQLANLSPYPESV
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
PINNLVPVAGTPLANTAPLDPFEFVRTIAVARITMPLTMVRLSAGREQMDEALQALCFAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GANSIFYGDKLLTTSNPQADRDRQLFERLGLKTQGARPAAQQAQ
CCCCEEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure
MMSIATIPVSSLRRAPAPTTAPQAPLRWQVEDIAALYELPFMDLLFRAQQVHREHFDANE
CCCEECCCHHHHHCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
VQLSTLLSIKTGGCAEDCGYCPQSTHFETEVKASKLMPLAEVIEAARAAKDQGATRFCMG
HHHHHHHHHCCCCCHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHH
AAWRSPKERDMERVTEIVREVRSLGLETCMTLGMLQAEQAQALKDAGLDYYNHNLDSAPE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHH
FYGDIISTRTYQDRLDTLGHVRQAGINVCCGGIVGMGESRLQRAGLIAQLANLSPYPESV
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCCCCCC
PINNLVPVAGTPLANTAPLDPFEFVRTIAVARITMPLTMVRLSAGREQMDEALQALCFAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
GANSIFYGDKLLTTSNPQADRDRQLFERLGLKTQGARPAAQQAQ
CCCCEEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA