Click here to switch to the map view.

The map label for this gene is yxeQ [H]

Identifier: 91787166

GI number: 91787166

Start: 1312445

End: 1313836

Strand: Reverse

Name: yxeQ [H]

Synonym: Bpro_1269

Alternate gene names: 91787166

Gene position: 1313836-1312445 (Counterclockwise)

Preceding gene: 91787167

Following gene: 91787165

Centisome position: 25.26

GC content: 65.8

Gene sequence:

>1392_bases
ATGGCCCGCAATACCCAGGTCGCTGCCGACCACAACGCTCCTCCCATCACACAAATCCTCGCGCAGTTCGTTGCCACCCA
TCCGTCGCGCGGATGGAGCGATGCGGTGGACCATGAGGCGCACCGCACCTTCATGAACTGGCTGGGCTGCGCCGTGGGTG
CGGCCCACCACGAAGCCGCCGACGCGGCGCTGGCCGCCGTGAAACTGCTGCAGCCCGCCGCGCAGGCCAGCGTGCTGGGC
CGGACGGAAAAAGTCGACATGGGCAGCGCGGCGCTGCTCAACGGCATCACCTCGCACACCTTTGACTTCGACGACACCCA
CCTCAAAACCATCATCCACCCCGCCGGCCCGGTGGCCTCTGCCGTGCTGGCGCTGGCCGAGCACACGGGCAGCTCGGGCC
GCGAGGTGATCGACGCGCTGGTGCTGGGCATAGACGTGTCCTGCCGCGTCGGCAACGCCATGTACCCCGAGCATTACGAC
CGCGGCTGGCACATCACCGGCTCCACCGGCACGCTGGGCGCCGCGGCGGCCTGCGCGCGCCTGCTCAAGCTGGATGTGCA
GAAGACCGCGATGGCGCTGGGCATTGCGGCTTCGCAGCCCGTCGGCATGCGCGAGCAGTTTGGCACCATGACCAAGCCCT
TTCACCCCGGCGGCGCCGCGCGTGCGGGACTGATGTCTGCCTTGCTGGCCAGCCAGGGCTTCACCGCCAGCCCCAAGGCC
CTTGAGGCGCCGCGCGGCATGATGCAGACCGTCTCGACCAAGAACGACTGGAACGAAATCACCCATGAGCTGGGCCAGCG
TTTCGAGATTTCGTTCAACAGCTACAAACCGTTTGCCTGCGGCATCGTGATTCACCCCAGCATTGATGCCTGCGCCCAAT
TGCGCGAAAGGTTTTCCAAAGAAGGCGTTACGGCCGAACAGATCGAGCGCGTCGAGCTCAAGGTGCACTCGCTGGTGCTG
GAGCTGACCGGCAAAAAGGAGCCCGCCGACGGCCTGCAGGCCAAGTTCAGCGTCTACCACGGCTGCGCCGCGGGGTTGAC
GTTTGGCCGCGCGGCTGAAGATGAGTTCTCCGACGAGATCGTCAACCGCGCCGACATGGTGGCGCTGCGCCGCAAGGTGG
TTGCGACGATCGACGATTCCATTGACGAGGCCAGTGCTGATGTGACTGCGGTACTCAAGGACGACCGCCGCGTGCATGTG
TTTGTCGAGCACGCGATCGGCTCACTGCAAAACCCGATGTCGGATGCCCAGCTGGAAGCGAAATTCCAGGACCTGTCTGA
CCCGATTCTGGGCGCCGGCAGAACCAGCGAATTGATCAACGCCTGCTGGGCCCTGGGCGCCGCCGCCAGCGTAGCGGCCG
TGGTTGCACTCGCCACCCCCGAAAGCCGATGA

Upstream 100 bases:

>100_bases
GTCATAAAACACGGCCTGCAGCCCGTATTTTCTGGCGCAGGCTGCTATTTATTTAATAGCTTATTGTCACTCTCATTAAC
CCCGCTTTTTCGGAACCCTC

Downstream 100 bases:

>100_bases
ATACAGCGGCATACGCCAGACCACGAATCGTCATTGCCGGAGACGCCGAACAGGCACTGCGACGGCTGGGCGACTGGCGC
AAGATTGATGCGCAGGCCGA

Product: MmgE/PrpD

Products: cis-2-Methylaconitate; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MARNTQVAADHNAPPITQILAQFVATHPSRGWSDAVDHEAHRTFMNWLGCAVGAAHHEAADAALAAVKLLQPAAQASVLG
RTEKVDMGSAALLNGITSHTFDFDDTHLKTIIHPAGPVASAVLALAEHTGSSGREVIDALVLGIDVSCRVGNAMYPEHYD
RGWHITGSTGTLGAAAACARLLKLDVQKTAMALGIAASQPVGMREQFGTMTKPFHPGGAARAGLMSALLASQGFTASPKA
LEAPRGMMQTVSTKNDWNEITHELGQRFEISFNSYKPFACGIVIHPSIDACAQLRERFSKEGVTAEQIERVELKVHSLVL
ELTGKKEPADGLQAKFSVYHGCAAGLTFGRAAEDEFSDEIVNRADMVALRRKVVATIDDSIDEASADVTAVLKDDRRVHV
FVEHAIGSLQNPMSDAQLEAKFQDLSDPILGAGRTSELINACWALGAAASVAAVVALATPESR

Sequences:

>Translated_463_residues
MARNTQVAADHNAPPITQILAQFVATHPSRGWSDAVDHEAHRTFMNWLGCAVGAAHHEAADAALAAVKLLQPAAQASVLG
RTEKVDMGSAALLNGITSHTFDFDDTHLKTIIHPAGPVASAVLALAEHTGSSGREVIDALVLGIDVSCRVGNAMYPEHYD
RGWHITGSTGTLGAAAACARLLKLDVQKTAMALGIAASQPVGMREQFGTMTKPFHPGGAARAGLMSALLASQGFTASPKA
LEAPRGMMQTVSTKNDWNEITHELGQRFEISFNSYKPFACGIVIHPSIDACAQLRERFSKEGVTAEQIERVELKVHSLVL
ELTGKKEPADGLQAKFSVYHGCAAGLTFGRAAEDEFSDEIVNRADMVALRRKVVATIDDSIDEASADVTAVLKDDRRVHV
FVEHAIGSLQNPMSDAQLEAKFQDLSDPILGAGRTSELINACWALGAAASVAAVVALATPESR
>Mature_462_residues
ARNTQVAADHNAPPITQILAQFVATHPSRGWSDAVDHEAHRTFMNWLGCAVGAAHHEAADAALAAVKLLQPAAQASVLGR
TEKVDMGSAALLNGITSHTFDFDDTHLKTIIHPAGPVASAVLALAEHTGSSGREVIDALVLGIDVSCRVGNAMYPEHYDR
GWHITGSTGTLGAAAACARLLKLDVQKTAMALGIAASQPVGMREQFGTMTKPFHPGGAARAGLMSALLASQGFTASPKAL
EAPRGMMQTVSTKNDWNEITHELGQRFEISFNSYKPFACGIVIHPSIDACAQLRERFSKEGVTAEQIERVELKVHSLVLE
LTGKKEPADGLQAKFSVYHGCAAGLTFGRAAEDEFSDEIVNRADMVALRRKVVATIDDSIDEASADVTAVLKDDRRVHVF
VEHAIGSLQNPMSDAQLEAKFQDLSDPILGAGRTSELINACWALGAAASVAAVVALATPESR

Specific function: Catalyzes The Dehydration Of 2-Methylcitrate To 2- Methyl-Cis-Aconitate. Also Seems To Be Responsible For The Residual Aconitase Activity Of The Acnab-Null Strain. [C]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Homo sapiens, GI310129732, Length=424, Percent_Identity=26.1792452830189, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI310110533, Length=424, Percent_Identity=26.1792452830189, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI310123058, Length=422, Percent_Identity=25.3554502369668, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: 4.2.1.79 [C]

Molecular weight: Translated: 49106; Mature: 48975

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNTQVAADHNAPPITQILAQFVATHPSRGWSDAVDHEAHRTFMNWLGCAVGAAHHEAA
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAALAAVKLLQPAAQASVLGRTEKVDMGSAALLNGITSHTFDFDDTHLKTIIHPAGPVAS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHCCCCCCCHHHHHHCCCCCHHHH
AVLALAEHTGSSGREVIDALVLGIDVSCRVGNAMYPEHYDRGWHITGSTGTLGAAAACAR
HHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
LLKLDVQKTAMALGIAASQPVGMREQFGTMTKPFHPGGAARAGLMSALLASQGFTASPKA
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
LEAPRGMMQTVSTKNDWNEITHELGQRFEISFNSYKPFACGIVIHPSIDACAQLRERFSK
HHCCHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHH
EGVTAEQIERVELKVHSLVLELTGKKEPADGLQAKFSVYHGCAAGLTFGRAAEDEFSDEI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VNRADMVALRRKVVATIDDSIDEASADVTAVLKDDRRVHVFVEHAIGSLQNPMSDAQLEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEHHHHHHHHCCCCCHHHHHH
KFQDLSDPILGAGRTSELINACWALGAAASVAAVVALATPESR
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARNTQVAADHNAPPITQILAQFVATHPSRGWSDAVDHEAHRTFMNWLGCAVGAAHHEAA
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAALAAVKLLQPAAQASVLGRTEKVDMGSAALLNGITSHTFDFDDTHLKTIIHPAGPVAS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCHHHCCCCCCCHHHHHHCCCCCHHHH
AVLALAEHTGSSGREVIDALVLGIDVSCRVGNAMYPEHYDRGWHITGSTGTLGAAAACAR
HHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
LLKLDVQKTAMALGIAASQPVGMREQFGTMTKPFHPGGAARAGLMSALLASQGFTASPKA
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
LEAPRGMMQTVSTKNDWNEITHELGQRFEISFNSYKPFACGIVIHPSIDACAQLRERFSK
HHCCHHHHHHHCCCCHHHHHHHHHCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHH
EGVTAEQIERVELKVHSLVLELTGKKEPADGLQAKFSVYHGCAAGLTFGRAAEDEFSDEI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VNRADMVALRRKVVATIDDSIDEASADVTAVLKDDRRVHVFVEHAIGSLQNPMSDAQLEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEHHHHHHHHCCCCCHHHHHH
KFQDLSDPILGAGRTSELINACWALGAAASVAAVVALATPESR
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Methylcitrate [C]

Specific reaction: 2-Methylcitrate --> cis-2-Methylaconitate + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]