Click here to switch to the map view.

The map label for this gene is kefF [H]

Identifier: 91787135

GI number: 91787135

Start: 1285188

End: 1285916

Strand: Reverse

Name: kefF [H]

Synonym: Bpro_1238

Alternate gene names: 91787135

Gene position: 1285916-1285188 (Counterclockwise)

Preceding gene: 91787138

Following gene: 91787134

Centisome position: 24.73

GC content: 63.51

Gene sequence:

>729_bases
ATGGACACCGTTCTCACAAACGCTCACAGGGCGGACAACGCCAGCAGTCAGGCTGCGAATACCGACATCTACGTCATCGC
GGCTCATCCGGCGTGGCGTGAATCGCGCGTGAATCGCCCCCTGATGGAGGCCGCGAAGACGCTGCCGCGCGTGCAGGTGC
AAGACCTGTATTCGAGCTACCCCGACTACGCCATTGACGTCGCCCGGGAGCAGGCCGCGCTGGAGCAGGCCCGGTTGGTG
GTGCTGCTGCACCCCATCCAGTGGTACTCGATGCCCGCCCTGCAAAAGCTCTGGCTGGACGATGTGTTGACGTATGGCTG
GGCCTATGGCCAGGGCGGACACGCCCTGGAAGGCAAGGATTTATGGCTGGTGGCGACCACCGGCGGGCCGGAGACTTCCT
ACCATCCGCAAGGCTACAACCGCTATTTTTTCGATGCTTTCCTGCCGCCCTACGAGCAAAGCGCCATCCTGTGCGGCATG
CGGTTCTTGCCGCCCATGGTGCTGCACGCTGCGCATCGGGTCAGCACGGAAGTCGTTGCCCAGCACCTGGCCACCTTCAG
CCAGCGCCTGCAAAGTTACCCCGACTGGCCCGAACTCGAAGAGCTTGAGCAATACCCCGCCTGCGCGGTGCCCATGAAAG
ACCGCTTGCCCAGGGACGAGGCCAGCATCGAAGCGGACCCGGAAAGCAAGGACGCAGGTACAACGACGAACGTGGGAGCC
ACCCGCTAA

Upstream 100 bases:

>100_bases
TGGATTGGCAATCACGACCACGGGCATACAACTCTCCTCTGGTGCTGAAGAACTTACCCAAGCGTAAGTTCACTGCTTGC
TTTTCCGCTATGGTAACGGC

Downstream 100 bases:

>100_bases
TGGAACACGCCCCCGCCTGGCTTACCAACAGCTTCATCTACCTGGCAGCTGCCGTTGTCGCCGTGCCGCTGGCCAAGAAA
TTCGGCCTGGGCTCCATCAT

Product: NAD(P)H dehydrogenase (quinone)

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDTVLTNAHRADNASSQAANTDIYVIAAHPAWRESRVNRPLMEAAKTLPRVQVQDLYSSYPDYAIDVAREQAALEQARLV
VLLHPIQWYSMPALQKLWLDDVLTYGWAYGQGGHALEGKDLWLVATTGGPETSYHPQGYNRYFFDAFLPPYEQSAILCGM
RFLPPMVLHAAHRVSTEVVAQHLATFSQRLQSYPDWPELEELEQYPACAVPMKDRLPRDEASIEADPESKDAGTTTNVGA
TR

Sequences:

>Translated_242_residues
MDTVLTNAHRADNASSQAANTDIYVIAAHPAWRESRVNRPLMEAAKTLPRVQVQDLYSSYPDYAIDVAREQAALEQARLV
VLLHPIQWYSMPALQKLWLDDVLTYGWAYGQGGHALEGKDLWLVATTGGPETSYHPQGYNRYFFDAFLPPYEQSAILCGM
RFLPPMVLHAAHRVSTEVVAQHLATFSQRLQSYPDWPELEELEQYPACAVPMKDRLPRDEASIEADPESKDAGTTTNVGA
TR
>Mature_242_residues
MDTVLTNAHRADNASSQAANTDIYVIAAHPAWRESRVNRPLMEAAKTLPRVQVQDLYSSYPDYAIDVAREQAALEQARLV
VLLHPIQWYSMPALQKLWLDDVLTYGWAYGQGGHALEGKDLWLVATTGGPETSYHPQGYNRYFFDAFLPPYEQSAILCGM
RFLPPMVLHAAHRVSTEVVAQHLATFSQRLQSYPDWPELEELEQYPACAVPMKDRLPRDEASIEADPESKDAGTTTNVGA
TR

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=143, Percent_Identity=35.6643356643357, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786231, Length=172, Percent_Identity=43.0232558139535, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789750, Length=174, Percent_Identity=41.3793103448276, Blast_Score=130, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 27160; Mature: 27160

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVLTNAHRADNASSQAANTDIYVIAAHPAWRESRVNRPLMEAAKTLPRVQVQDLYSSY
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHCCCHHHHHHHHCC
PDYAIDVAREQAALEQARLVVLLHPIQWYSMPALQKLWLDDVLTYGWAYGQGGHALEGKD
CCHHHHHHHHHHHHHHHCEEEEECCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEECCCC
LWLVATTGGPETSYHPQGYNRYFFDAFLPPYEQSAILCGMRFLPPMVLHAAHRVSTEVVA
EEEEEECCCCCCCCCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHLATFSQRLQSYPDWPELEELEQYPACAVPMKDRLPRDEASIEADPESKDAGTTTNVGA
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TR
CC
>Mature Secondary Structure
MDTVLTNAHRADNASSQAANTDIYVIAAHPAWRESRVNRPLMEAAKTLPRVQVQDLYSSY
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHCCCHHHHHHHHCC
PDYAIDVAREQAALEQARLVVLLHPIQWYSMPALQKLWLDDVLTYGWAYGQGGHALEGKD
CCHHHHHHHHHHHHHHHCEEEEECCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEECCCC
LWLVATTGGPETSYHPQGYNRYFFDAFLPPYEQSAILCGMRFLPPMVLHAAHRVSTEVVA
EEEEEECCCCCCCCCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHLATFSQRLQSYPDWPELEELEQYPACAVPMKDRLPRDEASIEADPESKDAGTTTNVGA
HHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA