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The map label for this gene is trmL [H]

Identifier: 91787138

GI number: 91787138

Start: 1287125

End: 1287676

Strand: Reverse

Name: trmL [H]

Synonym: Bpro_1241

Alternate gene names: 91787138

Gene position: 1287676-1287125 (Counterclockwise)

Preceding gene: 91787139

Following gene: 91787135

Centisome position: 24.76

GC content: 64.13

Gene sequence:

>552_bases
ATGGCCCCCACGTTCGCTCACTGCGTGTCGTACCCGGCTAGCAACGACAATTCAGCCATGTTCAATATTGTTCTCGTCGA
GCCTGAAATTCCGCCCAATACGGGCAACGTGATTCGCCTGGCGGCCAACACGGGTTGCCGTCTTCATCTGGTGGAGCCCT
TGGGCTTTTCGATGGACGACAAGCACATGCGGCGCGCCGGGCTGGATTATCACGAGTACGCCCAAGTGCTGCGCCATGCG
AGCTGGCAGGCGCTGCTGGACAGCCAGCGGCCGCCCGCCGGACGGCTCTTCGCCATGACGACGCATGGCGCCGGCAGCGT
GTACGACATGCGTTTCCTGCCGGGTGACTGGCTGGTGTTTGGCGCTGAAACCCGGGGCCTGGCGCCTGACGTACGGGAAT
CCTTTCCGATGGCCCAGCGCCTGAGGGTGCCCATGCAGCCCGGCCAGCGCAGCCTCAATTTGTCCAACACCGTCGCGGTG
ACGGTGTTTGAGGCCTGGCGCCAGAACGGTTTCGCCGGGTCACACCATCCAGCCGCCGCCGATCAGGGGTAA

Upstream 100 bases:

>100_bases
ACGTGATGACCAGCGGTGCCTCGCTGTTCACCGCCGCCCGGGCCCTGCGCGAAGCCGGTGCTGCCCACATCACGGCGGTG
GTGCTGGCGCGCACGGAGTA

Downstream 100 bases:

>100_bases
CGCCGCACCAGTGATTCGGCCACGCACACCGGCTTGGCCGAGCCTTCGCGCTCCACCGTTACCTCCCAGGTCATCTGCAT
GCCGGCGTTGTCGATCGGCT

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MAPTFAHCVSYPASNDNSAMFNIVLVEPEIPPNTGNVIRLAANTGCRLHLVEPLGFSMDDKHMRRAGLDYHEYAQVLRHA
SWQALLDSQRPPAGRLFAMTTHGAGSVYDMRFLPGDWLVFGAETRGLAPDVRESFPMAQRLRVPMQPGQRSLNLSNTVAV
TVFEAWRQNGFAGSHHPAAADQG

Sequences:

>Translated_183_residues
MAPTFAHCVSYPASNDNSAMFNIVLVEPEIPPNTGNVIRLAANTGCRLHLVEPLGFSMDDKHMRRAGLDYHEYAQVLRHA
SWQALLDSQRPPAGRLFAMTTHGAGSVYDMRFLPGDWLVFGAETRGLAPDVRESFPMAQRLRVPMQPGQRSLNLSNTVAV
TVFEAWRQNGFAGSHHPAAADQG
>Mature_182_residues
APTFAHCVSYPASNDNSAMFNIVLVEPEIPPNTGNVIRLAANTGCRLHLVEPLGFSMDDKHMRRAGLDYHEYAQVLRHAS
WQALLDSQRPPAGRLFAMTTHGAGSVYDMRFLPGDWLVFGAETRGLAPDVRESFPMAQRLRVPMQPGQRSLNLSNTVAVT
VFEAWRQNGFAGSHHPAAADQG

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=155, Percent_Identity=55.4838709677419, Blast_Score=186, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20141; Mature: 20010

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPTFAHCVSYPASNDNSAMFNIVLVEPEIPPNTGNVIRLAANTGCRLHLVEPLGFSMDD
CCCHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCH
KHMRRAGLDYHEYAQVLRHASWQALLDSQRPPAGRLFAMTTHGAGSVYDMRFLPGDWLVF
HHHHHHCCCHHHHHHHHHHCHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEE
GAETRGLAPDVRESFPMAQRLRVPMQPGQRSLNLSNTVAVTVFEAWRQNGFAGSHHPAAA
CCCCCCCCCCHHHHCCHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCCCCCC
DQG
CCC
>Mature Secondary Structure 
APTFAHCVSYPASNDNSAMFNIVLVEPEIPPNTGNVIRLAANTGCRLHLVEPLGFSMDD
CCHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCH
KHMRRAGLDYHEYAQVLRHASWQALLDSQRPPAGRLFAMTTHGAGSVYDMRFLPGDWLVF
HHHHHHCCCHHHHHHHHHHCHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEE
GAETRGLAPDVRESFPMAQRLRVPMQPGQRSLNLSNTVAVTVFEAWRQNGFAGSHHPAAA
CCCCCCCCCCHHHHCCHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCCCCCCC
DQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA