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The map label for this gene is dhaK [H]

Identifier: 91786601

GI number: 91786601

Start: 709115

End: 710098

Strand: Reverse

Name: dhaK [H]

Synonym: Bpro_0697

Alternate gene names: 91786601

Gene position: 710098-709115 (Counterclockwise)

Preceding gene: 91786602

Following gene: 91786600

Centisome position: 13.66

GC content: 64.94

Gene sequence:

>984_bases
ATGAAGAAATTCACCAACCTCCCGAGCGACCTCGTGTTTGAAAGCATACTGGGGTTCGGGGCCGCCCATGCCCAGCTGGT
GGCCGTGCATCTGGACCCGCTGTTCGTCACGCGCAAGAACCCCACGCCGTCCAAGGTAGCACTGATCTCCGGCGGTGGAT
CAGGCCACGAGCCGCTGCACACCGGCTTCGTCGGCACCGGGATGCTCGACGCCGCCTGTCCCGGCCAGGTGTTCACCTCG
CCAACACCCAACCAGATGCTGGCTGCGGCCAAGGCCGTCGGCGGCGAGGCGGGTGTACTTTTCATTGTCAAGAACTATGC
CGGCGACGTGATGAATTTCCAGATGGCCATGGACATGATGGACGGCGACTATGCCTGGGTCCTGGTCAACGACGACGTAT
CGGTCGACTCGGCCGGGCATCCCACGGGCCGCCGCGGCGTTGCCGGAACGCTGGTGGTCGAAAAGATGGTCGGCGCGGCC
GCGGAGCGCGGCGCCGACCTGGCCGCCTGCAAGCACCTGGGTGACCGCGTGAACGCCTGCACGGCCTCCATGGGGGTGGC
TTTCAGCAGCTGCACCGTGCCGGAGGCGGGCGTGCCGACTTTCGAGATCGGCCCTGACGAAATGGAGATCGGCGTGGGCA
TTCATGGCGAACCCGGGCGCCGGCGCGGGGCGTTCCAATCCGCCACCGTTATTGTTGAAGAGCTGATGGACGAAATCCTG
AAAGACTTGAACCCGAAGCAGGGGCAGGAGGTGCTGCTGCTCGTCAACGGCCTCGGCGGAACGCCGTTGATGGAGCTTTA
CCTGCTGTACCAGCTGGCCAGCGAGCGCCTGAGCCATGCCGGCCTGGTTCCGGCACGGTCGCTGGTAGGCAACTACACCA
CCTCGCTCGAAACGGCCGGCGCCTCCCTGACCGTGACGCTACTGAACGAAGAGTTGAAGGCGCTTTGGGATGCTCCGGTG
ACAACGCCGGCCTTGCGTTGGTAG

Upstream 100 bases:

>100_bases
GTGAGCAACCCCCGAATAGAAATCATCACGGCGGAGAACCTCGCGTGACAAAGCACGCCCGCGGTGACCATAATCGGCCC
ACTATCCACTACGAGCCGCG

Downstream 100 bases:

>100_bases
TGATTCTCCGGATGCGATCGGCTTGTGGTGGCGCGCCTGCAGCCGGATAGCCGAGGGCAACGGCAACGCCGTCTTGTATC
GCACCTGCTTCAACGCAAAA

Product: dihydroxyacetone kinase DhaK subunit 1

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKKFTNLPSDLVFESILGFGAAHAQLVAVHLDPLFVTRKNPTPSKVALISGGGSGHEPLHTGFVGTGMLDAACPGQVFTS
PTPNQMLAAAKAVGGEAGVLFIVKNYAGDVMNFQMAMDMMDGDYAWVLVNDDVSVDSAGHPTGRRGVAGTLVVEKMVGAA
AERGADLAACKHLGDRVNACTASMGVAFSSCTVPEAGVPTFEIGPDEMEIGVGIHGEPGRRRGAFQSATVIVEELMDEIL
KDLNPKQGQEVLLLVNGLGGTPLMELYLLYQLASERLSHAGLVPARSLVGNYTTSLETAGASLTVTLLNEELKALWDAPV
TTPALRW

Sequences:

>Translated_327_residues
MKKFTNLPSDLVFESILGFGAAHAQLVAVHLDPLFVTRKNPTPSKVALISGGGSGHEPLHTGFVGTGMLDAACPGQVFTS
PTPNQMLAAAKAVGGEAGVLFIVKNYAGDVMNFQMAMDMMDGDYAWVLVNDDVSVDSAGHPTGRRGVAGTLVVEKMVGAA
AERGADLAACKHLGDRVNACTASMGVAFSSCTVPEAGVPTFEIGPDEMEIGVGIHGEPGRRRGAFQSATVIVEELMDEIL
KDLNPKQGQEVLLLVNGLGGTPLMELYLLYQLASERLSHAGLVPARSLVGNYTTSLETAGASLTVTLLNEELKALWDAPV
TTPALRW
>Mature_327_residues
MKKFTNLPSDLVFESILGFGAAHAQLVAVHLDPLFVTRKNPTPSKVALISGGGSGHEPLHTGFVGTGMLDAACPGQVFTS
PTPNQMLAAAKAVGGEAGVLFIVKNYAGDVMNFQMAMDMMDGDYAWVLVNDDVSVDSAGHPTGRRGVAGTLVVEKMVGAA
AERGADLAACKHLGDRVNACTASMGVAFSSCTVPEAGVPTFEIGPDEMEIGVGIHGEPGRRRGAFQSATVIVEELMDEIL
KDLNPKQGQEVLLLVNGLGGTPLMELYLLYQLASERLSHAGLVPARSLVGNYTTSLETAGASLTVTLLNEELKALWDAPV
TTPALRW

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=290, Percent_Identity=41.7241379310345, Blast_Score=195, Evalue=4e-50,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=45.6338028169014, Blast_Score=307, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI17565018, Length=328, Percent_Identity=34.1463414634146, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6321055, Length=343, Percent_Identity=34.6938775510204, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323570, Length=339, Percent_Identity=33.0383480825959, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34287; Mature: 34287

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFTNLPSDLVFESILGFGAAHAQLVAVHLDPLFVTRKNPTPSKVALISGGGSGHEPLH
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECEEEEECCCCCCCEEEEEECCCCCCCCCC
TGFVGTGMLDAACPGQVFTSPTPNQMLAAAKAVGGEAGVLFIVKNYAGDVMNFQMAMDMM
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHC
DGDYAWVLVNDDVSVDSAGHPTGRRGVAGTLVVEKMVGAAAERGADLAACKHLGDRVNAC
CCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TASMGVAFSSCTVPEAGVPTFEIGPDEMEIGVGIHGEPGRRRGAFQSATVIVEELMDEIL
HHHHCCEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KDLNPKQGQEVLLLVNGLGGTPLMELYLLYQLASERLSHAGLVPARSLVGNYTTSLETAG
HHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHCC
ASLTVTLLNEELKALWDAPVTTPALRW
CEEEEEEHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKFTNLPSDLVFESILGFGAAHAQLVAVHLDPLFVTRKNPTPSKVALISGGGSGHEPLH
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECEEEEECCCCCCCEEEEEECCCCCCCCCC
TGFVGTGMLDAACPGQVFTSPTPNQMLAAAKAVGGEAGVLFIVKNYAGDVMNFQMAMDMM
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHC
DGDYAWVLVNDDVSVDSAGHPTGRRGVAGTLVVEKMVGAAAERGADLAACKHLGDRVNAC
CCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TASMGVAFSSCTVPEAGVPTFEIGPDEMEIGVGIHGEPGRRRGAFQSATVIVEELMDEIL
HHHHCCEECCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
KDLNPKQGQEVLLLVNGLGGTPLMELYLLYQLASERLSHAGLVPARSLVGNYTTSLETAG
HHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHCC
ASLTVTLLNEELKALWDAPVTTPALRW
CEEEEEEHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]