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The map label for this gene is gcvA [H]

Identifier: 91786569

GI number: 91786569

Start: 676470

End: 677447

Strand: Reverse

Name: gcvA [H]

Synonym: Bpro_0664

Alternate gene names: 91786569

Gene position: 677447-676470 (Counterclockwise)

Preceding gene: 91786570

Following gene: 91786560

Centisome position: 13.03

GC content: 66.46

Gene sequence:

>978_bases
ATGCTGCATTCGCAAACCCATTTACGCTCCCGGCCGATTTCAGCCGGCCAGCTGCGCGCCTTCGAGGCAGTCGCCCGCCA
CCTCAATTTCCGCGCCGCCTCCGAAGAGCTGGCGCTCACGCAGTCGGCCGTGAGCCGCCAGATCCAGGCGCTGGAAGAAG
ATGTCGGCGTCAGCCTGTTTCACCGTCACACCCGGGCGGTGGAGCTCACCGGTGCGGGCGGCACCCTGCTGCGGGCGGTC
ACACCGTCGCTGGAGCGCATTGACGGCGCGGTGCGGCAGATCCGCCAGAGCGCCGGGCGCAAAAGCGTTGCGCTCACCAC
CTTTGCGTCGTTTGCCTCGATGTGGCTGATTCCCCGGCTCGAGGTTTTTCAGCGCGAGAACCCCGACATCGACATCCGGA
TTGATGCGTCGGACGTGGCGGTGGACCTCGATGTTTCGGACCTGGACCTGGCTCTGCGCTATGCCCCGGCCAGCCACATG
CCGCCGCACGCCATCCGCCTGTTTGGCGAGCAGATCACGCCGGTGATCAGCCCCTGGCTGCTCAAGAGCGGCCCGCGCCT
GAAAAAGCCGGATGACCTGGCGCATTTCACCTTGATCGAAGCAGGCGACGCGCACCGCACGCACTTTGAATGGCTGACCT
GGCGCCGCTGGATGGACCAACACGCCACACCCAAGCTGGAGGCCAAGCGCTGGCTTTACTTCAACTACGCCTACCAGATG
GTGCAGGCGGCGCTGACGGGCCAGGGCGTGGTGCTGGCACGCCTGCCGCTGATTGCCGAAAGCCTGGCCTCGGGCGACCT
GGTCGAGCCCCTGCCGCACCTGCGCATGGAGTCCCCCATGGCTTACTGGCTGATCATCGGCCCACGCAATGTGGCGCGCC
CCGAAGTGCTGGCGTTCTGCGAGTGGCTCAAGGCGCAAGGCGCGCAGACCCGCAAGGCCATTGGCGATGTGCCCGACGCC
GACACGGTGGAGAAGTAG

Upstream 100 bases:

>100_bases
CTCGCCGGGGGGCTTCCCCGTCCAATGAAATCGCGGTACGCTATTCATTCCATAAACGCATCAATCACCCCACCTCCCAC
CCACCCCCACCCCACTCCGC

Downstream 100 bases:

>100_bases
GGCCCCCACGCTCCCCACTTCGTGTGGTTCGCTGCCCCCCGAGGGGGCGCTGCGCCTGCGGCCCGGCAAAGCCGGTTCCG
CGGCCCTGGCTGGGAAGATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MLHSQTHLRSRPISAGQLRAFEAVARHLNFRAASEELALTQSAVSRQIQALEEDVGVSLFHRHTRAVELTGAGGTLLRAV
TPSLERIDGAVRQIRQSAGRKSVALTTFASFASMWLIPRLEVFQRENPDIDIRIDASDVAVDLDVSDLDLALRYAPASHM
PPHAIRLFGEQITPVISPWLLKSGPRLKKPDDLAHFTLIEAGDAHRTHFEWLTWRRWMDQHATPKLEAKRWLYFNYAYQM
VQAALTGQGVVLARLPLIAESLASGDLVEPLPHLRMESPMAYWLIIGPRNVARPEVLAFCEWLKAQGAQTRKAIGDVPDA
DTVEK

Sequences:

>Translated_325_residues
MLHSQTHLRSRPISAGQLRAFEAVARHLNFRAASEELALTQSAVSRQIQALEEDVGVSLFHRHTRAVELTGAGGTLLRAV
TPSLERIDGAVRQIRQSAGRKSVALTTFASFASMWLIPRLEVFQRENPDIDIRIDASDVAVDLDVSDLDLALRYAPASHM
PPHAIRLFGEQITPVISPWLLKSGPRLKKPDDLAHFTLIEAGDAHRTHFEWLTWRRWMDQHATPKLEAKRWLYFNYAYQM
VQAALTGQGVVLARLPLIAESLASGDLVEPLPHLRMESPMAYWLIIGPRNVARPEVLAFCEWLKAQGAQTRKAIGDVPDA
DTVEK
>Mature_325_residues
MLHSQTHLRSRPISAGQLRAFEAVARHLNFRAASEELALTQSAVSRQIQALEEDVGVSLFHRHTRAVELTGAGGTLLRAV
TPSLERIDGAVRQIRQSAGRKSVALTTFASFASMWLIPRLEVFQRENPDIDIRIDASDVAVDLDVSDLDLALRYAPASHM
PPHAIRLFGEQITPVISPWLLKSGPRLKKPDDLAHFTLIEAGDAHRTHFEWLTWRRWMDQHATPKLEAKRWLYFNYAYQM
VQAALTGQGVVLARLPLIAESLASGDLVEPLPHLRMESPMAYWLIIGPRNVARPEVLAFCEWLKAQGAQTRKAIGDVPDA
DTVEK

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=33.6700336700337, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788706, Length=308, Percent_Identity=31.4935064935065, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786448, Length=255, Percent_Identity=36.8627450980392, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=28.4280936454849, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=34.8148148148148, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=309, Percent_Identity=23.9482200647249, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=120, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=307, Percent_Identity=27.0358306188925, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081978, Length=303, Percent_Identity=25.7425742574257, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367136, Length=119, Percent_Identity=36.1344537815126, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=30.2469135802469, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36377; Mature: 36377

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHSQTHLRSRPISAGQLRAFEAVARHLNFRAASEELALTQSAVSRQIQALEEDVGVSLF
CCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHH
HRHTRAVELTGAGGTLLRAVTPSLERIDGAVRQIRQSAGRKSVALTTFASFASMWLIPRL
HHCCCEEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
EVFQRENPDIDIRIDASDVAVDLDVSDLDLALRYAPASHMPPHAIRLFGEQITPVISPWL
HHHHCCCCCEEEEEECCCEEEEECCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LKSGPRLKKPDDLAHFTLIEAGDAHRTHFEWLTWRRWMDQHATPKLEAKRWLYFNYAYQM
HCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHH
VQAALTGQGVVLARLPLIAESLASGDLVEPLPHLRMESPMAYWLIIGPRNVARPEVLAFC
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHH
EWLKAQGAQTRKAIGDVPDADTVEK
HHHHHCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLHSQTHLRSRPISAGQLRAFEAVARHLNFRAASEELALTQSAVSRQIQALEEDVGVSLF
CCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHH
HRHTRAVELTGAGGTLLRAVTPSLERIDGAVRQIRQSAGRKSVALTTFASFASMWLIPRL
HHCCCEEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
EVFQRENPDIDIRIDASDVAVDLDVSDLDLALRYAPASHMPPHAIRLFGEQITPVISPWL
HHHHCCCCCEEEEEECCCEEEEECCHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LKSGPRLKKPDDLAHFTLIEAGDAHRTHFEWLTWRRWMDQHATPKLEAKRWLYFNYAYQM
HCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHH
VQAALTGQGVVLARLPLIAESLASGDLVEPLPHLRMESPMAYWLIIGPRNVARPEVLAFC
HHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEEECCCCCCCHHHHHHH
EWLKAQGAQTRKAIGDVPDADTVEK
HHHHHCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]