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The map label for this gene is yafC [C]

Identifier: 91786549

GI number: 91786549

Start: 653897

End: 654787

Strand: Reverse

Name: yafC [C]

Synonym: Bpro_0644

Alternate gene names: 91786549

Gene position: 654787-653897 (Counterclockwise)

Preceding gene: 91786552

Following gene: 91786548

Centisome position: 12.59

GC content: 64.53

Gene sequence:

>891_bases
ATGGACAAGCTCAGGGCCATGCAGGCCTTCATTCATATTGCCGAAGAAGGCAGCCTGACCGCTGCGGCCCGCGTCATGGA
GTCGTCGCTGCCCGCCATGGTGCGCACGCTGGCCGGCTTCGAAGCGCAACTGGGCGTGCGCCTGTTCAACCGGACCACGC
GACGCATTTCGCTGACCGAGGAAGGCCGGCGGCACCTGGAGAGTTGCCGGCAATTGCTGGCCGCGCTGGACGATGCGGAA
GCAGCGCTCACGGCGGAGGCCGGCGAACCAACGGGCCACCTGACCATCACCGCGCCCGTGCTGTTCGGGCAAATGCATGT
TGCCCCTGCCGTGACGCGCTTTGTGCAGCAATACGAAAAGATGCGCTGCAGCATGGTGCTGCTGGACCGCGTGGTCAACC
TGCTGGAAGAAGGCATTGACGTAGGCATCCGCATTGGCGAGCCCGAAGACTCCACGCTGGTCGCGCAGACGGTCGGCCAC
ATCCGGCGCCTGGTGGTAGCCAGCCCCGACTGGCTGAAACGCCATGGCACGCCGCGGCATCCCAAAGACCTGCTGAAGGC
CAACTGCGTGCGCGTGACCGAGCATGCCCTCACATGGGGGCCGTTCAATGACCAGGGCCGGCCACTGCGCCTGGCCGTCA
GTGGTAATCTGGAGTTCAACCAGAATGCGCCGGCCGTTGCGGCTTGCGTGGCCGGCGCGGGCCTGGGCATGTTTCTGTCC
TACCAGGTGGCGCCGTTTCTGCAAAGCAAACAACTGTGCACCGTGCTGGAGGATTTTGAGCCGGCGGTTCGCCCGATCAA
CGTGGTCTACCCGCACGCCCGGCTGTTGCCCATGCGCACCCGGGTGTTCATCGAGTGGATCAAGAAGGAACTCAAGGACT
TCCAGTCCTGA

Upstream 100 bases:

>100_bases
GTGGATTCTGTTTGATCCGTTAATTTGAATAAATAGCTGACATAGGATTTAACTAATCCTTAAATCGGATTAATTTCAAG
CACGGGGTTACCATGGCGCC

Downstream 100 bases:

>100_bases
AGACTGAAAAAGGAAAGAGCGCATGCAAACTTTTGATTTTGACCTTTTCGTCATCGGCGGCGGCAGCGGCGGCGTGCGCG
CCGCCCGCATGTCGGCACAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDKLRAMQAFIHIAEEGSLTAAARVMESSLPAMVRTLAGFEAQLGVRLFNRTTRRISLTEEGRRHLESCRQLLAALDDAE
AALTAEAGEPTGHLTITAPVLFGQMHVAPAVTRFVQQYEKMRCSMVLLDRVVNLLEEGIDVGIRIGEPEDSTLVAQTVGH
IRRLVVASPDWLKRHGTPRHPKDLLKANCVRVTEHALTWGPFNDQGRPLRLAVSGNLEFNQNAPAVAACVAGAGLGMFLS
YQVAPFLQSKQLCTVLEDFEPAVRPINVVYPHARLLPMRTRVFIEWIKKELKDFQS

Sequences:

>Translated_296_residues
MDKLRAMQAFIHIAEEGSLTAAARVMESSLPAMVRTLAGFEAQLGVRLFNRTTRRISLTEEGRRHLESCRQLLAALDDAE
AALTAEAGEPTGHLTITAPVLFGQMHVAPAVTRFVQQYEKMRCSMVLLDRVVNLLEEGIDVGIRIGEPEDSTLVAQTVGH
IRRLVVASPDWLKRHGTPRHPKDLLKANCVRVTEHALTWGPFNDQGRPLRLAVSGNLEFNQNAPAVAACVAGAGLGMFLS
YQVAPFLQSKQLCTVLEDFEPAVRPINVVYPHARLLPMRTRVFIEWIKKELKDFQS
>Mature_296_residues
MDKLRAMQAFIHIAEEGSLTAAARVMESSLPAMVRTLAGFEAQLGVRLFNRTTRRISLTEEGRRHLESCRQLLAALDDAE
AALTAEAGEPTGHLTITAPVLFGQMHVAPAVTRFVQQYEKMRCSMVLLDRVVNLLEEGIDVGIRIGEPEDSTLVAQTVGH
IRRLVVASPDWLKRHGTPRHPKDLLKANCVRVTEHALTWGPFNDQGRPLRLAVSGNLEFNQNAPAVAACVAGAGLGMFLS
YQVAPFLQSKQLCTVLEDFEPAVRPINVVYPHARLLPMRTRVFIEWIKKELKDFQS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=30.0699300699301, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=29.2253521126761, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=28.5234899328859, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=28.6206896551724, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=26.5734265734266, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=28.8659793814433, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=27.4305555555556, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=23.4323432343234, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693105, Length=157, Percent_Identity=27.3885350318471, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=23.3676975945017, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789173, Length=234, Percent_Identity=24.3589743589744, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32851; Mature: 32851

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLRAMQAFIHIAEEGSLTAAARVMESSLPAMVRTLAGFEAQLGVRLFNRTTRRISLTE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHEEECHH
EGRRHLESCRQLLAALDDAEAALTAEAGEPTGHLTITAPVLFGQMHVAPAVTRFVQQYEK
HHHHHHHHHHHHHHHHCCHHHHEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
MRCSMVLLDRVVNLLEEGIDVGIRIGEPEDSTLVAQTVGHIRRLVVASPDWLKRHGTPRH
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PKDLLKANCVRVTEHALTWGPFNDQGRPLRLAVSGNLEFNQNAPAVAACVAGAGLGMFLS
HHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHCCHHHHHH
YQVAPFLQSKQLCTVLEDFEPAVRPINVVYPHARLLPMRTRVFIEWIKKELKDFQS
HHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDKLRAMQAFIHIAEEGSLTAAARVMESSLPAMVRTLAGFEAQLGVRLFNRTTRRISLTE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHEEECHH
EGRRHLESCRQLLAALDDAEAALTAEAGEPTGHLTITAPVLFGQMHVAPAVTRFVQQYEK
HHHHHHHHHHHHHHHHCCHHHHEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
MRCSMVLLDRVVNLLEEGIDVGIRIGEPEDSTLVAQTVGHIRRLVVASPDWLKRHGTPRH
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCC
PKDLLKANCVRVTEHALTWGPFNDQGRPLRLAVSGNLEFNQNAPAVAACVAGAGLGMFLS
HHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHCCHHHHHH
YQVAPFLQSKQLCTVLEDFEPAVRPINVVYPHARLLPMRTRVFIEWIKKELKDFQS
HHHHHHHHHHHHHHHHHHCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]