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The map label for this gene is gcvA [H]

Identifier: 91786552

GI number: 91786552

Start: 656583

End: 657506

Strand: Reverse

Name: gcvA [H]

Synonym: Bpro_0647

Alternate gene names: 91786552

Gene position: 657506-656583 (Counterclockwise)

Preceding gene: 91786557

Following gene: 91786549

Centisome position: 12.64

GC content: 67.75

Gene sequence:

>924_bases
ATGCTGAACAGTTCTTCCTCCAGCGCGCTGCGCGCCATGTCGCTTGATGCGATACGCGGCTTCGAAGCGGCCGCGCGTCA
CCTGAGCTTCACCGCTGCGGCCGAAGAGCTGTTCATCACGCAATCGGCCGTGAGCAAGCAGGTCAAGAGCCTGGAAGACG
CGCTGGGCGCAGCGCTGTTCTTGCGCGGCGGCAAGGGGCTGAGCCTGACGCCCGAGGGCCGGCAGCTTTATGAAGCGGCC
CGTGCGTCGCTGGCGCAACTGGCCTCGGCCGTCGACCGCCTGCTGGCCGTAGACCGGGTGTCGGTGTCGATCACCACCAC
GCCGTCTTTCGCCGCGCTGTGGCTGGCGCCCAAGCTGGCGAAGTTCCAGCTGCTGGAGCCCGCCATTGACGTGCGCGTCG
ACGCCTCGGAAGCCAAGGTGAACCTGGAGCGCGAAGGCATCGACCTGGCCGTGCGGCTAAGCCCGCCTCCGCCCGGAGGC
GAAGGCCCGCCGCCCTTGCTGCAAGAACAAGCCCTGCTGGTGGCCGCGCCGGCCGTCGCGGCGCGCATCAGCGCTGTCGC
CGACATCCTGCACACGCCGCTGCTTGTTTATTACGACCCGGCCACGCGCTTTTCGTGGATGTCGTGGACGGCCTGGTACG
AACGGCTGGAGCTGAAGCAGTCGACCCAGCAGCCCTGCCTGTATTTTTCGCAGTACGAGCATGTGCTGACCGCCGCCGTT
CAGGGCGCCGGCATCGCCATAGGGCGCACGCCGCTGGTGCTGCCCCTGCTGGGCAGCGGCCAGCTCAAGGTGGTCTTGCC
CGGCCAGACGGTCGAGGGCATGGCTTACCGCATCGTCACCTCAGCCCACAGCGCGGATCGGCCGGCTGTGCAGAAATTTC
GCGCATGGCTGGAGCGAGAGCTCGCGCTGGAAGCCATCGGCTGA

Upstream 100 bases:

>100_bases
TGCATGGCGGACTCCTTGGAAAGTGTGGTTTGATTCTTCCCTGTACCCGGCCCGCGCGGCCGGATGGTTTGTCATCCGGG
GTATTCCAATCATTCATGCC

Downstream 100 bases:

>100_bases
GCTCGTGTCAACGCTACAGATCAGGCCCAGGTGCCCGTGGTCTCAAGCACCGGCGACAGCTCCGCCTCGCCCCAGCGCAG
GGGCAATGAGCCCTTCACCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MLNSSSSSALRAMSLDAIRGFEAAARHLSFTAAAEELFITQSAVSKQVKSLEDALGAALFLRGGKGLSLTPEGRQLYEAA
RASLAQLASAVDRLLAVDRVSVSITTTPSFAALWLAPKLAKFQLLEPAIDVRVDASEAKVNLEREGIDLAVRLSPPPPGG
EGPPPLLQEQALLVAAPAVAARISAVADILHTPLLVYYDPATRFSWMSWTAWYERLELKQSTQQPCLYFSQYEHVLTAAV
QGAGIAIGRTPLVLPLLGSGQLKVVLPGQTVEGMAYRIVTSAHSADRPAVQKFRAWLERELALEAIG

Sequences:

>Translated_307_residues
MLNSSSSSALRAMSLDAIRGFEAAARHLSFTAAAEELFITQSAVSKQVKSLEDALGAALFLRGGKGLSLTPEGRQLYEAA
RASLAQLASAVDRLLAVDRVSVSITTTPSFAALWLAPKLAKFQLLEPAIDVRVDASEAKVNLEREGIDLAVRLSPPPPGG
EGPPPLLQEQALLVAAPAVAARISAVADILHTPLLVYYDPATRFSWMSWTAWYERLELKQSTQQPCLYFSQYEHVLTAAV
QGAGIAIGRTPLVLPLLGSGQLKVVLPGQTVEGMAYRIVTSAHSADRPAVQKFRAWLERELALEAIG
>Mature_307_residues
MLNSSSSSALRAMSLDAIRGFEAAARHLSFTAAAEELFITQSAVSKQVKSLEDALGAALFLRGGKGLSLTPEGRQLYEAA
RASLAQLASAVDRLLAVDRVSVSITTTPSFAALWLAPKLAKFQLLEPAIDVRVDASEAKVNLEREGIDLAVRLSPPPPGG
EGPPPLLQEQALLVAAPAVAARISAVADILHTPLLVYYDPATRFSWMSWTAWYERLELKQSTQQPCLYFSQYEHVLTAAV
QGAGIAIGRTPLVLPLLGSGQLKVVLPGQTVEGMAYRIVTSAHSADRPAVQKFRAWLERELALEAIG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=33.4494773519164, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=32.013201320132, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=29.5918367346939, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=26.5993265993266, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787128, Length=278, Percent_Identity=26.6187050359712, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789440, Length=251, Percent_Identity=26.6932270916335, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081978, Length=193, Percent_Identity=29.5336787564767, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=282, Percent_Identity=24.113475177305, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32907; Mature: 32907

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNSSSSSALRAMSLDAIRGFEAAARHLSFTAAAEELFITQSAVSKQVKSLEDALGAALF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LRGGKGLSLTPEGRQLYEAARASLAQLASAVDRLLAVDRVSVSITTTPSFAALWLAPKLA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHH
KFQLLEPAIDVRVDASEAKVNLEREGIDLAVRLSPPPPGGEGPPPLLQEQALLVAAPAVA
HHHHHCCCCCEEEECCHHEEEEEECCCEEEEEECCCCCCCCCCCCHHHCCHHEEECHHHH
ARISAVADILHTPLLVYYDPATRFSWMSWTAWYERLELKQSTQQPCLYFSQYEHVLTAAV
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QGAGIAIGRTPLVLPLLGSGQLKVVLPGQTVEGMAYRIVTSAHSADRPAVQKFRAWLERE
CCCCEEECCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LALEAIG
HHHHCCC
>Mature Secondary Structure
MLNSSSSSALRAMSLDAIRGFEAAARHLSFTAAAEELFITQSAVSKQVKSLEDALGAALF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
LRGGKGLSLTPEGRQLYEAARASLAQLASAVDRLLAVDRVSVSITTTPSFAALWLAPKLA
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHH
KFQLLEPAIDVRVDASEAKVNLEREGIDLAVRLSPPPPGGEGPPPLLQEQALLVAAPAVA
HHHHHCCCCCEEEECCHHEEEEEECCCEEEEEECCCCCCCCCCCCHHHCCHHEEECHHHH
ARISAVADILHTPLLVYYDPATRFSWMSWTAWYERLELKQSTQQPCLYFSQYEHVLTAAV
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QGAGIAIGRTPLVLPLLGSGQLKVVLPGQTVEGMAYRIVTSAHSADRPAVQKFRAWLERE
CCCCEEECCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LALEAIG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]