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The map label for this gene is gatA [H]

Identifier: 91786465

GI number: 91786465

Start: 558115

End: 559521

Strand: Direct

Name: gatA [H]

Synonym: Bpro_0559

Alternate gene names: 91786465

Gene position: 558115-559521 (Clockwise)

Preceding gene: 91786464

Following gene: 91786466

Centisome position: 10.73

GC content: 72.0

Gene sequence:

>1407_bases
ATGATAGACAAGGACTTGTGGCGCCTGGGCGCCGTTGAGCTGGGTCAGCGCTTTCGCGAGCGCTCCCTCACGCCGCTGGC
GGTGGCCCAGGCCTGCCTGGCACGGCTGGAAGCGGTGAACCCGCGGCTGAACGCGGTCATTGCACGGCGTGACGAGCAGT
TTCTGGCGGAGGCCAAGGCCGCCACGGAGCGCCATGCGCGCGGACAGCCGCTGTCCGCGCTGGACGGCATTCCGCTCACC
GTGAAGGACAGCCTCTACCTGGCCGACCTGCCGACCACCTGCGGCACGGCGGCGCTGCGCGAGCACCGGCCCGGCCACGA
CGAGCTGGCCGCCGCGCGGGCCCGCGCCGGTGGTGCCCTGATCATCGGTAAGACCAATGTGCCCGAGTTCGCCAATGACG
GCTACACGGCCAACCCGCTGTTCGGCGTTACCGGCAATCCCTGGGCTCCGGAGCTCACGCCCGGCGGCTCCAGCGGCGGT
GCGGTGGCCGCCGTGGCCGCCGGCATTGCGCCGCTGGCGATCGCGCAGGACGGCGGCGGCTCGATCCGCCGCCCGGCCTC
GCACACCGGGCTGGTCGGCCTCAAGCCCTCGCTCAGCGCCTGGCCGCGCGAGCACGCGCTGCCCGGCCTGCTGCTGGACT
TTGACTGCATCGGCCCGGTGGCGCGCACGGTGGCCGACGCGCGCTTGCTGTTCGAAGCCCTGCGTGGTCCCTCGCCGGCG
GACCGTTCCTCGATGGCGGCGGCCTGGGCAGCGGCGCAGCCCCGGCCTGCGGGCTCGCTGCGCGTGCTCTACGTCGAGCG
GCTGAACGCGAACCCGCTGGACCCGCAGATCGCCGCCAGTTGCCTTCACGCCGTGCAGCGACTCGCTGCGCTGGGCCACC
GCGTCGAGCCTGGCGAGTTGCCGCTGGACGTGCGGTTCATGACCGAGGCGTGGCCGCAAATCGGCCAGGTGGGCCTGGCC
GCGATGTTTGACCAGCATCCCGATTGGGAAGCCCAGGCCAGCCCCAAATACCGCGAGGCAGCCGAGGCAGGCCGTCGCGT
CTCGGGCTCGCGGGTTTGGCAGATCATGGAAGGGGTGAGGCGCCTGCGCCGCGAAAGCGCCGCGCTGTTCGAGCGTATCG
ACGTGGTGGTCATGCCCGCTGCCGCCGCGCTGCCCTGGCCGGCTCACGAGCCCTATCCGTCTGTCATCGACGGCCAGCCC
GTTGGCCCGCGCGGCCATGCAGCCTACACCGGTTGGGTCAACGCGGCCGGCCTGCCGGGCCTGGCCGTGCCCTGCGAGCC
GTCACGCGAAGGCCTGCCCATCGGCATGCAAATGATTGGTGCTTACGGCGCCGATGATCAGCTGCTCGATCTCGGCGCCA
GCTACGAGGCGGCGCATCCATGGAGCGGGCGCTGGCCTGCCCTTTAA

Upstream 100 bases:

>100_bases
CTGCCCTACGCCCTGATCATGATCCTGTTCACCTTGCTCATCATGGTGTGGCCGCAGATTGTCACCTGGCTGCCGGCCCA
CATGTGAGGACTGAACACGA

Downstream 100 bases:

>100_bases
GACAGTTAGTTAACAATTTCAATACATCCAAAGAGACAACACCATGCAAAGACGTCATTTCATCCAGGCAACCGGCGCAG
GCGCCGCAGTGCTGGCCCTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MIDKDLWRLGAVELGQRFRERSLTPLAVAQACLARLEAVNPRLNAVIARRDEQFLAEAKAATERHARGQPLSALDGIPLT
VKDSLYLADLPTTCGTAALREHRPGHDELAAARARAGGALIIGKTNVPEFANDGYTANPLFGVTGNPWAPELTPGGSSGG
AVAAVAAGIAPLAIAQDGGGSIRRPASHTGLVGLKPSLSAWPREHALPGLLLDFDCIGPVARTVADARLLFEALRGPSPA
DRSSMAAAWAAAQPRPAGSLRVLYVERLNANPLDPQIAASCLHAVQRLAALGHRVEPGELPLDVRFMTEAWPQIGQVGLA
AMFDQHPDWEAQASPKYREAAEAGRRVSGSRVWQIMEGVRRLRRESAALFERIDVVVMPAAAALPWPAHEPYPSVIDGQP
VGPRGHAAYTGWVNAAGLPGLAVPCEPSREGLPIGMQMIGAYGADDQLLDLGASYEAAHPWSGRWPAL

Sequences:

>Translated_468_residues
MIDKDLWRLGAVELGQRFRERSLTPLAVAQACLARLEAVNPRLNAVIARRDEQFLAEAKAATERHARGQPLSALDGIPLT
VKDSLYLADLPTTCGTAALREHRPGHDELAAARARAGGALIIGKTNVPEFANDGYTANPLFGVTGNPWAPELTPGGSSGG
AVAAVAAGIAPLAIAQDGGGSIRRPASHTGLVGLKPSLSAWPREHALPGLLLDFDCIGPVARTVADARLLFEALRGPSPA
DRSSMAAAWAAAQPRPAGSLRVLYVERLNANPLDPQIAASCLHAVQRLAALGHRVEPGELPLDVRFMTEAWPQIGQVGLA
AMFDQHPDWEAQASPKYREAAEAGRRVSGSRVWQIMEGVRRLRRESAALFERIDVVVMPAAAALPWPAHEPYPSVIDGQP
VGPRGHAAYTGWVNAAGLPGLAVPCEPSREGLPIGMQMIGAYGADDQLLDLGASYEAAHPWSGRWPAL
>Mature_468_residues
MIDKDLWRLGAVELGQRFRERSLTPLAVAQACLARLEAVNPRLNAVIARRDEQFLAEAKAATERHARGQPLSALDGIPLT
VKDSLYLADLPTTCGTAALREHRPGHDELAAARARAGGALIIGKTNVPEFANDGYTANPLFGVTGNPWAPELTPGGSSGG
AVAAVAAGIAPLAIAQDGGGSIRRPASHTGLVGLKPSLSAWPREHALPGLLLDFDCIGPVARTVADARLLFEALRGPSPA
DRSSMAAAWAAAQPRPAGSLRVLYVERLNANPLDPQIAASCLHAVQRLAALGHRVEPGELPLDVRFMTEAWPQIGQVGLA
AMFDQHPDWEAQASPKYREAAEAGRRVSGSRVWQIMEGVRRLRRESAALFERIDVVVMPAAAALPWPAHEPYPSVIDGQP
VGPRGHAAYTGWVNAAGLPGLAVPCEPSREGLPIGMQMIGAYGADDQLLDLGASYEAAHPWSGRWPAL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=24.4897959183673, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI222831590, Length=259, Percent_Identity=35.5212355212355, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI166795287, Length=294, Percent_Identity=29.9319727891156, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=23.5294117647059, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=227, Percent_Identity=33.0396475770925, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=456, Percent_Identity=25.219298245614, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=235, Percent_Identity=31.063829787234, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=235, Percent_Identity=28.5106382978723, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=194, Percent_Identity=32.4742268041237, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=194, Percent_Identity=32.4742268041237, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=393, Percent_Identity=26.2086513994911, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=278, Percent_Identity=23.7410071942446, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=200, Percent_Identity=30.5, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=448, Percent_Identity=27.2321428571429, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550774, Length=497, Percent_Identity=25.3521126760563, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=497, Percent_Identity=25.3521126760563, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648437, Length=497, Percent_Identity=25.3521126760563, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=26.0869565217391, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=27.5100401606426, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=457, Percent_Identity=26.0393873085339, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=26.1044176706827, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=26.1044176706827, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=26.1044176706827, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=26.1044176706827, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648441, Length=423, Percent_Identity=24.3498817966903, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648439, Length=423, Percent_Identity=24.3498817966903, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 49592; Mature: 49592

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKDLWRLGAVELGQRFRERSLTPLAVAQACLARLEAVNPRLNAVIARRDEQFLAEAKA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ATERHARGQPLSALDGIPLTVKDSLYLADLPTTCGTAALREHRPGHDELAAARARAGGAL
HHHHHCCCCCCHHHCCCCEEEECCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCEE
IIGKTNVPEFANDGYTANPLFGVTGNPWAPELTPGGSSGGAVAAVAAGIAPLAIAQDGGG
EEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCC
SIRRPASHTGLVGLKPSLSAWPREHALPGLLLDFDCIGPVARTVADARLLFEALRGPSPA
CCCCCCCCCCEEECCCCHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
DRSSMAAAWAAAQPRPAGSLRVLYVERLNANPLDPQIAASCLHAVQRLAALGHRVEPGEL
CHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PLDVRFMTEAWPQIGQVGLAAMFDQHPDWEAQASPKYREAAEAGRRVSGSRVWQIMEGVR
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
RLRRESAALFERIDVVVMPAAAALPWPAHEPYPSVIDGQPVGPRGHAAYTGWVNAAGLPG
HHHHHHHHHHHHHCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
LAVPCEPSREGLPIGMQMIGAYGADDQLLDLGASYEAAHPWSGRWPAL
CCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDKDLWRLGAVELGQRFRERSLTPLAVAQACLARLEAVNPRLNAVIARRDEQFLAEAKA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ATERHARGQPLSALDGIPLTVKDSLYLADLPTTCGTAALREHRPGHDELAAARARAGGAL
HHHHHCCCCCCHHHCCCCEEEECCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCEE
IIGKTNVPEFANDGYTANPLFGVTGNPWAPELTPGGSSGGAVAAVAAGIAPLAIAQDGGG
EEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCC
SIRRPASHTGLVGLKPSLSAWPREHALPGLLLDFDCIGPVARTVADARLLFEALRGPSPA
CCCCCCCCCCEEECCCCHHCCCHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCC
DRSSMAAAWAAAQPRPAGSLRVLYVERLNANPLDPQIAASCLHAVQRLAALGHRVEPGEL
CHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PLDVRFMTEAWPQIGQVGLAAMFDQHPDWEAQASPKYREAAEAGRRVSGSRVWQIMEGVR
CEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
RLRRESAALFERIDVVVMPAAAALPWPAHEPYPSVIDGQPVGPRGHAAYTGWVNAAGLPG
HHHHHHHHHHHHHCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
LAVPCEPSREGLPIGMQMIGAYGADDQLLDLGASYEAAHPWSGRWPAL
CCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA