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The map label for this gene is mdlA [H]

Identifier: 91786345

GI number: 91786345

Start: 451431

End: 452582

Strand: Reverse

Name: mdlA [H]

Synonym: Bpro_0435

Alternate gene names: 91786345

Gene position: 452582-451431 (Counterclockwise)

Preceding gene: 91786347

Following gene: 91786344

Centisome position: 8.7

GC content: 65.02

Gene sequence:

>1152_bases
ATGAAGACGACAACCAGTTCCACGCCCAGCGACCGCATCACCTGGGTCCGGATATCGTCCTGCTACCTGCCACTCGCCAC
ACCCATCAGCGACGCCAAGGTATTGACCGGCCGCCAGAAGCCGATGACCGAGATTGCCATCCTGTTCGCGGAAATCGAGA
CAGCAGGTGGCCATCAGGGTCTGGGCTTCAGCTACTCCAAGCGGGCCGGCGGGCCAGGCCAGTTTGCCCATGCCAGGGAG
ATCGCACCGGCGCTCATCGGCGAGGACCCCAGCGACATCGCCAAACTCTGGGACAAGCTCTGCTGGGCCGGTGCCTCCGC
GGGACGCAGTGGTCTGTCGACGCAGGCCATCGGCGCCTTTGACGTTGCGTTGTGGGACCTGAAGGCCAAACGCGCCGGTC
TCTCGCTGGCCAAGTTGCTCGGATCCTACCGCGACTCGGTGCGCTGCTACAACACCTCGGGCGGTTTTCTGCACACACCC
ATCGACCAGCTCATGGTCAATGCATCGGCATCCATTGAGCGCGGTATCGGTGGCATCAAGCTCAAGGTTGGCCAGCCGGA
CGGCGCGCTGGACATCGCCCGCGTGACGGCCGTGCGCAAACACCTGGGTGACGCCGTGCCCCTCATGGTCGATGCCAACC
AGCAATGGGACCGGCCAACCGCCCAGCGCATGTGCCGCATCTTCGAGCCGTTCAACCTGGTCTGGATCGAGGAACCGCTG
GATGCCTACGACCATGAAGGCCATGCGGCGCTGGCGCTGCAGTTTGACACGCCGATTGCGACCGGCGAGATGCTGACCAG
CGCGGCCGAGCACGGAGACCTGATCCGCCATCGGGCGGCCGACTATTTGATGCCCGATGCGCCACGGGTCGGCGGCATCA
CCCCCTTCCTGAAGATCGCTTCGCTGGCCGAACACGCGGGGTTGATGCTGGCGCCGCACTTCGCGATGGAGTTGCATGTG
CATCTGGCCGCGGCTTACCCGCGCGAACCCTGGGTCGAGCATTTCGAGTGGCTGGAGCCGCTGTTCAATGAGCGCATCGA
GATCAGGGATGGGCGGATGCTGGTGCCGACCAGGCCTGGCCTGGGCCTCACCCTGAGCGGGCAGGTCAAGGCCTGGACCC
GAGAAGAAGCGCAAGTCGGCACACGCCCGTAG

Upstream 100 bases:

>100_bases
CGGGCACAAGCACCGGGCGGCCGTGGTGACCACAGCCGGGTTGAAGCTCGACTGACACCTGAACGCCGAATGAACGTTCC
CGCTCACCCCTGAAGAGTCC

Downstream 100 bases:

>100_bases
CGTGCGAACGGCCATTCGCGACATTGAATGCAGGTCTTGCCAGGCTACCCACAATATGGTTCAGTTAAGCCCTTGCCCCT
GGTTTGTCATCCCGGACTTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKTTTSSTPSDRITWVRISSCYLPLATPISDAKVLTGRQKPMTEIAILFAEIETAGGHQGLGFSYSKRAGGPGQFAHARE
IAPALIGEDPSDIAKLWDKLCWAGASAGRSGLSTQAIGAFDVALWDLKAKRAGLSLAKLLGSYRDSVRCYNTSGGFLHTP
IDQLMVNASASIERGIGGIKLKVGQPDGALDIARVTAVRKHLGDAVPLMVDANQQWDRPTAQRMCRIFEPFNLVWIEEPL
DAYDHEGHAALALQFDTPIATGEMLTSAAEHGDLIRHRAADYLMPDAPRVGGITPFLKIASLAEHAGLMLAPHFAMELHV
HLAAAYPREPWVEHFEWLEPLFNERIEIRDGRMLVPTRPGLGLTLSGQVKAWTREEAQVGTRP

Sequences:

>Translated_383_residues
MKTTTSSTPSDRITWVRISSCYLPLATPISDAKVLTGRQKPMTEIAILFAEIETAGGHQGLGFSYSKRAGGPGQFAHARE
IAPALIGEDPSDIAKLWDKLCWAGASAGRSGLSTQAIGAFDVALWDLKAKRAGLSLAKLLGSYRDSVRCYNTSGGFLHTP
IDQLMVNASASIERGIGGIKLKVGQPDGALDIARVTAVRKHLGDAVPLMVDANQQWDRPTAQRMCRIFEPFNLVWIEEPL
DAYDHEGHAALALQFDTPIATGEMLTSAAEHGDLIRHRAADYLMPDAPRVGGITPFLKIASLAEHAGLMLAPHFAMELHV
HLAAAYPREPWVEHFEWLEPLFNERIEIRDGRMLVPTRPGLGLTLSGQVKAWTREEAQVGTRP
>Mature_383_residues
MKTTTSSTPSDRITWVRISSCYLPLATPISDAKVLTGRQKPMTEIAILFAEIETAGGHQGLGFSYSKRAGGPGQFAHARE
IAPALIGEDPSDIAKLWDKLCWAGASAGRSGLSTQAIGAFDVALWDLKAKRAGLSLAKLLGSYRDSVRCYNTSGGFLHTP
IDQLMVNASASIERGIGGIKLKVGQPDGALDIARVTAVRKHLGDAVPLMVDANQQWDRPTAQRMCRIFEPFNLVWIEEPL
DAYDHEGHAALALQFDTPIATGEMLTSAAEHGDLIRHRAADYLMPDAPRVGGITPFLKIASLAEHAGLMLAPHFAMELHV
HLAAAYPREPWVEHFEWLEPLFNERIEIRDGRMLVPTRPGLGLTLSGQVKAWTREEAQVGTRP

Specific function: Unknown

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=386, Percent_Identity=24.3523316062176, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI186972148, Length=225, Percent_Identity=28, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994953, Length=276, Percent_Identity=30.7971014492754, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI226510960, Length=371, Percent_Identity=28.0323450134771, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787864, Length=333, Percent_Identity=27.3273273273273, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 41724; Mature: 41724

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTTSSTPSDRITWVRISSCYLPLATPISDAKVLTGRQKPMTEIAILFAEIETAGGHQG
CCCCCCCCCCCCEEEEEEECEECCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCC
LGFSYSKRAGGPGQFAHAREIAPALIGEDPSDIAKLWDKLCWAGASAGRSGLSTQAIGAF
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
DVALWDLKAKRAGLSLAKLLGSYRDSVRCYNTSGGFLHTPIDQLMVNASASIERGIGGIK
HHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCCEEECCHHHHHHCCCCHHHCCCCCEE
LKVGQPDGALDIARVTAVRKHLGDAVPLMVDANQQWDRPTAQRMCRIFEPFNLVWIEEPL
EEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCH
DAYDHEGHAALALQFDTPIATGEMLTSAAEHGDLIRHRAADYLMPDAPRVGGITPFLKIA
HHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SLAEHAGLMLAPHFAMELHVHLAAAYPREPWVEHFEWLEPLFNERIEIRDGRMLVPTRPG
HHHHHCCEEEECCHHEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCC
LGLTLSGQVKAWTREEAQVGTRP
CCEEECCCEEECCCHHCCCCCCC
>Mature Secondary Structure
MKTTTSSTPSDRITWVRISSCYLPLATPISDAKVLTGRQKPMTEIAILFAEIETAGGHQG
CCCCCCCCCCCCEEEEEEECEECCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCC
LGFSYSKRAGGPGQFAHAREIAPALIGEDPSDIAKLWDKLCWAGASAGRSGLSTQAIGAF
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
DVALWDLKAKRAGLSLAKLLGSYRDSVRCYNTSGGFLHTPIDQLMVNASASIERGIGGIK
HHHHHHHHHHHCCHHHHHHHHHHHCCEEEECCCCCEEECCHHHHHHCCCCHHHCCCCCEE
LKVGQPDGALDIARVTAVRKHLGDAVPLMVDANQQWDRPTAQRMCRIFEPFNLVWIEEPL
EEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCH
DAYDHEGHAALALQFDTPIATGEMLTSAAEHGDLIRHRAADYLMPDAPRVGGITPFLKIA
HHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SLAEHAGLMLAPHFAMELHVHLAAAYPREPWVEHFEWLEPLFNERIEIRDGRMLVPTRPG
HHHHHCCEEEECCHHEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCEEEEECCCC
LGLTLSGQVKAWTREEAQVGTRP
CCEEECCCEEECCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]