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The map label for this gene is gcvA [H]

Identifier: 91786347

GI number: 91786347

Start: 454261

End: 455160

Strand: Reverse

Name: gcvA [H]

Synonym: Bpro_0437

Alternate gene names: 91786347

Gene position: 455160-454261 (Counterclockwise)

Preceding gene: 91786348

Following gene: 91786345

Centisome position: 8.75

GC content: 65.11

Gene sequence:

>900_bases
ATGGCCGCGCCGGCGAGAATTCCACCGATTCAGTGCCTGCTGACGTTTGAAGCGCTGGCACGGCTGCGCAGCGTGACCCA
GGCGGCCGAGGAACTCTGCGTGACACCCAGCGCCGTGAGCCACCGGGTCAAGCAGCTGGAGCAAATCATCGGCACCAAGC
TGTTTGGCCGGGCTGATTTTTCATTGACCACCGAAGGCAGCGAATACCTGGCGCATGTGCGCGAGGGCCTCGCCACGCTG
CAGAAGTTTCCCGGCACCGCCGGCCAGAATGGCACAACCGGCAAGCGCAAGCTGCGGCTGGCGATCACGCCCACGTTTGC
CCGTTCGATTCTGATTCCACGGTTGCGGCAATTCACCGAGGCCTATCCCGAGATTGACCTGACCCTGCAGGTCTCGATTC
CCCTGCTGGATGTGGTGGCCGAAGATGCCGACCTGATCGTGCGCTTCGGCACCGGGCGTTATGCCGACATGGAACATGTG
TGCCTGGCCCATGACGACGTCACCCCGCTGGCCTCGCCGGCCTTTGTACGCGAGCACGGTCCCTTTGAAGCGGCGGAAGA
CCTGGAAGGCGAGAGCCTGCTGCGCTCCCCGCTGGAGCCCTGGCGCACCTGGTTTGTCGCCAACAACCTGGACTGGCCCG
AGCCGCAAGACGGCTCCCAGTTCAATGACATCGGCCTCATGTGCGATGCGGCCGCGGCGGGCATGGGCATCGCACTCATC
CGCCTCAAGCTGGGCGCACCCTGGCTGGACAATGGCTCGCTGGTGCGCCTGGGCAACAACGCGGTCTTCGGCCGCAACGT
TCCGAGCCCGCACGCCCACTATCTGTGCTGGCGTACCGGCACCATGGACCGCTGGGAATGTGCGGCCTTCGCGGACTGGC
TGCGCAAGAGCCTGACCTGA

Upstream 100 bases:

>100_bases
GCGACACCGAAAGCCAGGTGCTGGCGCGCGCCGTCAAGTGGCACAGCGAGCATCGCGTGCTGCTCAACGGCCACAAGACC
GTGATTTTCAAGTAACCACC

Downstream 100 bases:

>100_bases
TCCGTACCACAGCTTGCCCTCCCTGTACTGAGCTGAGGCGTCAACCCGAAGAAAACATCAAGCCTGGCTGGCACCGCCCC
CGGTTGACTGCCTCTCGACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAAPARIPPIQCLLTFEALARLRSVTQAAEELCVTPSAVSHRVKQLEQIIGTKLFGRADFSLTTEGSEYLAHVREGLATL
QKFPGTAGQNGTTGKRKLRLAITPTFARSILIPRLRQFTEAYPEIDLTLQVSIPLLDVVAEDADLIVRFGTGRYADMEHV
CLAHDDVTPLASPAFVREHGPFEAAEDLEGESLLRSPLEPWRTWFVANNLDWPEPQDGSQFNDIGLMCDAAAAGMGIALI
RLKLGAPWLDNGSLVRLGNNAVFGRNVPSPHAHYLCWRTGTMDRWECAAFADWLRKSLT

Sequences:

>Translated_299_residues
MAAPARIPPIQCLLTFEALARLRSVTQAAEELCVTPSAVSHRVKQLEQIIGTKLFGRADFSLTTEGSEYLAHVREGLATL
QKFPGTAGQNGTTGKRKLRLAITPTFARSILIPRLRQFTEAYPEIDLTLQVSIPLLDVVAEDADLIVRFGTGRYADMEHV
CLAHDDVTPLASPAFVREHGPFEAAEDLEGESLLRSPLEPWRTWFVANNLDWPEPQDGSQFNDIGLMCDAAAAGMGIALI
RLKLGAPWLDNGSLVRLGNNAVFGRNVPSPHAHYLCWRTGTMDRWECAAFADWLRKSLT
>Mature_298_residues
AAPARIPPIQCLLTFEALARLRSVTQAAEELCVTPSAVSHRVKQLEQIIGTKLFGRADFSLTTEGSEYLAHVREGLATLQ
KFPGTAGQNGTTGKRKLRLAITPTFARSILIPRLRQFTEAYPEIDLTLQVSIPLLDVVAEDADLIVRFGTGRYADMEHVC
LAHDDVTPLASPAFVREHGPFEAAEDLEGESLLRSPLEPWRTWFVANNLDWPEPQDGSQFNDIGLMCDAAAAGMGIALIR
LKLGAPWLDNGSLVRLGNNAVFGRNVPSPHAHYLCWRTGTMDRWECAAFADWLRKSLT

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=32.1917808219178, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786448, Length=251, Percent_Identity=33.0677290836653, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=30.5084745762712, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI145693193, Length=188, Percent_Identity=28.1914893617021, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=28.3737024221453, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787128, Length=245, Percent_Identity=27.3469387755102, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081978, Length=184, Percent_Identity=28.2608695652174, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32961; Mature: 32830

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPARIPPIQCLLTFEALARLRSVTQAAEELCVTPSAVSHRVKQLEQIIGTKLFGRADF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
SLTTEGSEYLAHVREGLATLQKFPGTAGQNGTTGKRKLRLAITPTFARSILIPRLRQFTE
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
AYPEIDLTLQVSIPLLDVVAEDADLIVRFGTGRYADMEHVCLAHDDVTPLASPAFVREHG
HCCCEEEEEEECCCHHHHHHCCCCEEEEECCCCCCCCHHEEEECCCCCCCCCCHHHHCCC
PFEAAEDLEGESLLRSPLEPWRTWFVANNLDWPEPQDGSQFNDIGLMCDAAAAGMGIALI
CCCHHHCCCHHHHHHCCHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
RLKLGAPWLDNGSLVRLGNNAVFGRNVPSPHAHYLCWRTGTMDRWECAAFADWLRKSLT
EEECCCCCCCCCCEEEECCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAPARIPPIQCLLTFEALARLRSVTQAAEELCVTPSAVSHRVKQLEQIIGTKLFGRADF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCC
SLTTEGSEYLAHVREGLATLQKFPGTAGQNGTTGKRKLRLAITPTFARSILIPRLRQFTE
EEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
AYPEIDLTLQVSIPLLDVVAEDADLIVRFGTGRYADMEHVCLAHDDVTPLASPAFVREHG
HCCCEEEEEEECCCHHHHHHCCCCEEEEECCCCCCCCHHEEEECCCCCCCCCCHHHHCCC
PFEAAEDLEGESLLRSPLEPWRTWFVANNLDWPEPQDGSQFNDIGLMCDAAAAGMGIALI
CCCHHHCCCHHHHHHCCHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEE
RLKLGAPWLDNGSLVRLGNNAVFGRNVPSPHAHYLCWRTGTMDRWECAAFADWLRKSLT
EEECCCCCCCCCCEEEECCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]