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The map label for this gene is bam [H]

Identifier: 91786323

GI number: 91786323

Start: 425327

End: 426727

Strand: Reverse

Name: bam [H]

Synonym: Bpro_0413

Alternate gene names: 91786323

Gene position: 426727-425327 (Counterclockwise)

Preceding gene: 91786324

Following gene: 91786317

Centisome position: 8.21

GC content: 70.31

Gene sequence:

>1401_bases
ATGTCTGACATCTGGCGTCTCTCCGCATCCCGGGTCGCGAACCTGATCGCCCAGCGCAAGCTGTCTGCCACCGAAGCGGC
CGAATCGGCGCTGGCGCGGCTGGCCGTCGTCAATCCGCGCCTGAATGCCGTGGTGGACTACCGGCCCGACGAAGTGCTCC
GGCGCGCGGGCGCCATCGATGCGGCGATGGCGCGCGGCGAGAACCCGGGACCGCTGGCCGGCGTGCCCGTCACGGTCAAG
ATCAACATCGACCAGGCAGGCTTCGCGACCAGCAATGGGCTGACCTTGCAGAAGGATCTGATCGCCAGCACCAACAGCCC
CGTGGTGGACAACCTGCTGAAAGCCGGTGCGGTCATCGTGGGGCGCACCAACACGCCGGCCTTCAGCTACCGCTGGTTCA
CCAGCAACCGGCTGCACGGCCGCACGCTCAATCCGCGCAATACCGCGCTCACGCCCGGCGGTTCGTCCGGCGGCGCCGCA
TCGGCGGTGGCGGCCGGTATCGGCCACATCGCCCATGGGACCGACATCGCCGGCTCGATCCGCTACCCGGCTTACGCCTG
CGGCGTGCATGGCCTGCGGCCCACGCTGGGCCGCGTGGCGGCGTACAACGCCTCCAGCCCCGAACGCACGATCGGCGGGC
AGGTGACCGCCGTGTCGGGGCCGATCGCACGTACCGTGCACGATGTCCGCCTCGCGCTGGCAGCGATGGCGGCGCCGGAT
GCGCGCGACCCGTGGTGGGTGCCCGCGCCGCTCACCGGTCCGGTGCTGCCGCGCATGGCCGCCGTCTGCGTACGCCCGGA
CGGCCTCAACACATGCCCCGAGGTCGTCGCCGCGCTGCTCGACGCGGCCGACCGCCTGCGCGACGCGGGCTGGACCGTGG
ACGAACTCGACGCGCTGCCACCGATCAAGGAGGCCTGCACCAGCCAGATCACGCTGTGGATGGGCGACGGCTACGAGGCG
ATGGTGCGTGCCGCCGAGCGCGAGGGCGACGAAGGCGCCATCACCGCGCTGGCCGGACAGGCCAAAATCGCAGGGGCCAT
GACCGCCGAGAAACTGTCCGGTGCGCTGATCCGCCGCGCCACCATCACCAGGTCATGGCAGCAGTTCATGGAACGGTATC
CGGTCGTGCTGATGCCGGCTTGCGCAGAGTTGCCCTTTCCGGCCGATCTCGACCTGCAAGGGCCCGAGGCCTATGCCCGC
GTATGGGCAGCCCAGATGCCGATGATCGGTCTGCCCCTCACAGGCCTGCCCTCTTTCGTCGTGTCCACGGGCATGCTTGG
GGCCAGCCCCGTCGGCGTGCAGATCGTGGCCGGGCGCTTCCGTGAGGATTTGTGCCTGGCGGCGGGCGAGGCTATCGAGG
CCAGGGGAGCACCGCCCATGCCAGCGGATGTCAGCGACTGA

Upstream 100 bases:

>100_bases
CGCTGAAGATCCGTCCGCCGCTGGTGTTCTCCGAAGCCAACGCGGACATGCTGGTCGAGACGCTGGACAGCGTTCTGACA
ACCCTGTAAACAGGATTCCC

Downstream 100 bases:

>100_bases
CGCCAGTACTGACTACTTGAGCTCGATCTCGACGAAGACGAACTCGTGGTCGTTCGCGTTGATGACGTTGTGCTCGACAC
CGGTCAGCCGTGTGTACGAC

Product: amidase

Products: NA

Alternate protein names: IAH; Indole-3-acetamide hydrolase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDIWRLSASRVANLIAQRKLSATEAAESALARLAVVNPRLNAVVDYRPDEVLRRAGAIDAAMARGENPGPLAGVPVTVK
INIDQAGFATSNGLTLQKDLIASTNSPVVDNLLKAGAVIVGRTNTPAFSYRWFTSNRLHGRTLNPRNTALTPGGSSGGAA
SAVAAGIGHIAHGTDIAGSIRYPAYACGVHGLRPTLGRVAAYNASSPERTIGGQVTAVSGPIARTVHDVRLALAAMAAPD
ARDPWWVPAPLTGPVLPRMAAVCVRPDGLNTCPEVVAALLDAADRLRDAGWTVDELDALPPIKEACTSQITLWMGDGYEA
MVRAAEREGDEGAITALAGQAKIAGAMTAEKLSGALIRRATITRSWQQFMERYPVVLMPACAELPFPADLDLQGPEAYAR
VWAAQMPMIGLPLTGLPSFVVSTGMLGASPVGVQIVAGRFREDLCLAAGEAIEARGAPPMPADVSD

Sequences:

>Translated_466_residues
MSDIWRLSASRVANLIAQRKLSATEAAESALARLAVVNPRLNAVVDYRPDEVLRRAGAIDAAMARGENPGPLAGVPVTVK
INIDQAGFATSNGLTLQKDLIASTNSPVVDNLLKAGAVIVGRTNTPAFSYRWFTSNRLHGRTLNPRNTALTPGGSSGGAA
SAVAAGIGHIAHGTDIAGSIRYPAYACGVHGLRPTLGRVAAYNASSPERTIGGQVTAVSGPIARTVHDVRLALAAMAAPD
ARDPWWVPAPLTGPVLPRMAAVCVRPDGLNTCPEVVAALLDAADRLRDAGWTVDELDALPPIKEACTSQITLWMGDGYEA
MVRAAEREGDEGAITALAGQAKIAGAMTAEKLSGALIRRATITRSWQQFMERYPVVLMPACAELPFPADLDLQGPEAYAR
VWAAQMPMIGLPLTGLPSFVVSTGMLGASPVGVQIVAGRFREDLCLAAGEAIEARGAPPMPADVSD
>Mature_465_residues
SDIWRLSASRVANLIAQRKLSATEAAESALARLAVVNPRLNAVVDYRPDEVLRRAGAIDAAMARGENPGPLAGVPVTVKI
NIDQAGFATSNGLTLQKDLIASTNSPVVDNLLKAGAVIVGRTNTPAFSYRWFTSNRLHGRTLNPRNTALTPGGSSGGAAS
AVAAGIGHIAHGTDIAGSIRYPAYACGVHGLRPTLGRVAAYNASSPERTIGGQVTAVSGPIARTVHDVRLALAAMAAPDA
RDPWWVPAPLTGPVLPRMAAVCVRPDGLNTCPEVVAALLDAADRLRDAGWTVDELDALPPIKEACTSQITLWMGDGYEAM
VRAAEREGDEGAITALAGQAKIAGAMTAEKLSGALIRRATITRSWQQFMERYPVVLMPACAELPFPADLDLQGPEAYARV
WAAQMPMIGLPLTGLPSFVVSTGMLGASPVGVQIVAGRFREDLCLAAGEAIEARGAPPMPADVSD

Specific function: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid (IAA) [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=244, Percent_Identity=35.655737704918, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI222831590, Length=514, Percent_Identity=25.6809338521401, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI166795287, Length=216, Percent_Identity=34.2592592592593, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=488, Percent_Identity=24.3852459016393, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=162, Percent_Identity=37.6543209876543, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=292, Percent_Identity=27.3972602739726, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=302, Percent_Identity=28.1456953642384, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=200, Percent_Identity=32, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=200, Percent_Identity=32, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=231, Percent_Identity=30.7359307359307, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=323, Percent_Identity=27.2445820433437, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648435, Length=247, Percent_Identity=34.8178137651822, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648437, Length=247, Percent_Identity=34.8178137651822, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550774, Length=247, Percent_Identity=34.8178137651822, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=26.9938650306748, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=26.9938650306748, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=26.9938650306748, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=26.9938650306748, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=25.6916996047431, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21356731, Length=484, Percent_Identity=27.4793388429752, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078093, Length=507, Percent_Identity=25.6410256410256, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=34.8571428571429, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=34.8571428571429, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648113, Length=468, Percent_Identity=25.8547008547009, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 48651; Mature: 48520

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIWRLSASRVANLIAQRKLSATEAAESALARLAVVNPRLNAVVDYRPDEVLRRAGAID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCHH
AAMARGENPGPLAGVPVTVKINIDQAGFATSNGLTLQKDLIASTNSPVVDNLLKAGAVIV
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHCCCCHHHHHHHHCCEEEE
GRTNTPAFSYRWFTSNRLHGRTLNPRNTALTPGGSSGGAASAVAAGIGHIAHGTDIAGSI
ECCCCCCEEEEEEECCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
RYPAYACGVHGLRPTLGRVAAYNASSPERTIGGQVTAVSGPIARTVHDVRLALAAMAAPD
CCCHHHHCCCCCCHHHHHHEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCC
ARDPWWVPAPLTGPVLPRMAAVCVRPDGLNTCPEVVAALLDAADRLRDAGWTVDELDALP
CCCCCEECCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
PIKEACTSQITLWMGDGYEAMVRAAEREGDEGAITALAGQAKIAGAMTAEKLSGALIRRA
CHHHHHCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
TITRSWQQFMERYPVVLMPACAELPFPADLDLQGPEAYARVWAAQMPMIGLPLTGLPSFV
HHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
VSTGMLGASPVGVQIVAGRFREDLCLAAGEAIEARGAPPMPADVSD
HHHCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SDIWRLSASRVANLIAQRKLSATEAAESALARLAVVNPRLNAVVDYRPDEVLRRAGAID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCHH
AAMARGENPGPLAGVPVTVKINIDQAGFATSNGLTLQKDLIASTNSPVVDNLLKAGAVIV
HHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHCCCCHHHHHHHHCCEEEE
GRTNTPAFSYRWFTSNRLHGRTLNPRNTALTPGGSSGGAASAVAAGIGHIAHGTDIAGSI
ECCCCCCEEEEEEECCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
RYPAYACGVHGLRPTLGRVAAYNASSPERTIGGQVTAVSGPIARTVHDVRLALAAMAAPD
CCCHHHHCCCCCCHHHHHHEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCC
ARDPWWVPAPLTGPVLPRMAAVCVRPDGLNTCPEVVAALLDAADRLRDAGWTVDELDALP
CCCCCEECCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCC
PIKEACTSQITLWMGDGYEAMVRAAEREGDEGAITALAGQAKIAGAMTAEKLSGALIRRA
CHHHHHCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
TITRSWQQFMERYPVVLMPACAELPFPADLDLQGPEAYARVWAAQMPMIGLPLTGLPSFV
HHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
VSTGMLGASPVGVQIVAGRFREDLCLAAGEAIEARGAPPMPADVSD
HHHCCCCCCCCCCEEEHHHHHHHHHHHCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]