Click here to switch to the map view.

The map label for this gene is yogA [H]

Identifier: 91786014

GI number: 91786014

Start: 105757

End: 106758

Strand: Reverse

Name: yogA [H]

Synonym: Bpro_0102

Alternate gene names: 91786014

Gene position: 106758-105757 (Counterclockwise)

Preceding gene: 91786015

Following gene: 91786013

Centisome position: 2.05

GC content: 60.78

Gene sequence:

>1002_bases
ATGAAAGCATTTATTCTTGAGCGATATGGAAAAAAGCGCGCGTTGCGCTTGGCGGACATGCCGACTCCGGAGTTGCGCGA
CGACGAGGTCTTGGTCCAGGTCCATGCCGCTGGCGTGAACCTGCTGGACTCCAAGATCCGGGATGGAGAGTTCAAGCTCA
TCCTGCCCTATCGCCTGCCCCTCATCCTGGGCCACGACGTCGCTGGGGTGATCGTCAAGGTCGGCCCGCAAGTGCGGCAG
TTCAAGCTGGGCGATGAGGTCTATGCGCGGCCGGATGATTTTCGGATCGGTACATTCGCTGAGTTCGTCCCGGTCAAGGA
GGCATCGCTTGCGCTCAAGCCCAAGGGCCTCACGATGGAAGAAGCCGCTTCCATTCCCCTGGTGGCTTTGACGGCTTGGC
AGGCACTGGTCGAGAAGGCCAACCTGAAAAAGGGGCAGAAGGTCTTCATCCAGGCTGGCTCGGGCGGCGTAGGCACTTTT
GCCATCCAGTTGGCCAAGCATCTGGGCGCAACCGTCGCCACGACGACGAGCACGACCAATGTCGCGCTCGTGAAGAGTCT
GGGCGCGGACGTCGTCATCGACTACAAGACGCAGGACTTTGAGGATGTCTTGCGCGACTACGACGTGGTCCTGAACAGCC
AGGACGGCAAGACGCTCGAAAAATCCCTCCGCGTGCTCAAGGGCGGCGGGAAGCTCATCTCCATCTCCGGTCCGCCTGAT
CCCGAATTCGGCAAGGAAAGCGGGGCCCCCGGGTTCGTGAAACTGGTCATGCGGCTGCTGAGCTCAGGCATCAGACGCAA
GGCAAGGAGCCGCGGCGTCAGCTTCTCGTTCCTCTTCATGAAGGCGAACGGCAGCCAACTGCGCGAGGTCACCCGTCTTT
TCGATGCTGGCGTCATCCGCCCCGTGATGGATCGGGTGTTCCCGTTCGAATCAACCAACGAGGCCATGGCCTACGTCGAA
GCAGGCCGCGCCAAGGGCAAGGTCGTCGTCAAGATCAAGTGA

Upstream 100 bases:

>100_bases
CCTGACCGACAACGGCAAGAAGCTCAGCGTACGCGGCTACATCGGCGTGCCAATGCTGGGCCGCTCGCAAACCTGGGTAC
GCCAGGAATAAGGAAAAACC

Downstream 100 bases:

>100_bases
GTTTGTTCATGTCAGTGTTGCTGGAAAACCTGAAAGCCGGCCGCTGAATCTGTTTGTCCCCCACTTTGGAGATTCCTATG
AAAATCGAGAATTCTGTTGT

Product: zinc-binding alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKAFILERYGKKRALRLADMPTPELRDDEVLVQVHAAGVNLLDSKIRDGEFKLILPYRLPLILGHDVAGVIVKVGPQVRQ
FKLGDEVYARPDDFRIGTFAEFVPVKEASLALKPKGLTMEEAASIPLVALTAWQALVEKANLKKGQKVFIQAGSGGVGTF
AIQLAKHLGATVATTTSTTNVALVKSLGADVVIDYKTQDFEDVLRDYDVVLNSQDGKTLEKSLRVLKGGGKLISISGPPD
PEFGKESGAPGFVKLVMRLLSSGIRRKARSRGVSFSFLFMKANGSQLREVTRLFDAGVIRPVMDRVFPFESTNEAMAYVE
AGRAKGKVVVKIK

Sequences:

>Translated_333_residues
MKAFILERYGKKRALRLADMPTPELRDDEVLVQVHAAGVNLLDSKIRDGEFKLILPYRLPLILGHDVAGVIVKVGPQVRQ
FKLGDEVYARPDDFRIGTFAEFVPVKEASLALKPKGLTMEEAASIPLVALTAWQALVEKANLKKGQKVFIQAGSGGVGTF
AIQLAKHLGATVATTTSTTNVALVKSLGADVVIDYKTQDFEDVLRDYDVVLNSQDGKTLEKSLRVLKGGGKLISISGPPD
PEFGKESGAPGFVKLVMRLLSSGIRRKARSRGVSFSFLFMKANGSQLREVTRLFDAGVIRPVMDRVFPFESTNEAMAYVE
AGRAKGKVVVKIK
>Mature_333_residues
MKAFILERYGKKRALRLADMPTPELRDDEVLVQVHAAGVNLLDSKIRDGEFKLILPYRLPLILGHDVAGVIVKVGPQVRQ
FKLGDEVYARPDDFRIGTFAEFVPVKEASLALKPKGLTMEEAASIPLVALTAWQALVEKANLKKGQKVFIQAGSGGVGTF
AIQLAKHLGATVATTTSTTNVALVKSLGADVVIDYKTQDFEDVLRDYDVVLNSQDGKTLEKSLRVLKGGGKLISISGPPD
PEFGKESGAPGFVKLVMRLLSSGIRRKARSRGVSFSFLFMKANGSQLREVTRLFDAGVIRPVMDRVFPFESTNEAMAYVE
AGRAKGKVVVKIK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=355, Percent_Identity=36.6197183098592, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI22538446, Length=342, Percent_Identity=29.8245614035088, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI22538444, Length=342, Percent_Identity=29.8245614035088, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI194239674, Length=342, Percent_Identity=28.3625730994152, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI13236495, Length=342, Percent_Identity=28.3625730994152, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18379349, Length=321, Percent_Identity=27.1028037383178, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI24308257, Length=350, Percent_Identity=26.8571428571429, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI194239676, Length=209, Percent_Identity=32.5358851674641, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI41872631, Length=235, Percent_Identity=29.7872340425532, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI28557745, Length=343, Percent_Identity=29.1545189504373, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI156627571, Length=349, Percent_Identity=24.3553008595989, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI90669511, Length=164, Percent_Identity=33.5365853658537, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI67078404, Length=249, Percent_Identity=26.5060240963855, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1790485, Length=258, Percent_Identity=30.6201550387597, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71987554, Length=369, Percent_Identity=29.5392953929539, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17507255, Length=208, Percent_Identity=34.6153846153846, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71988145, Length=219, Percent_Identity=28.310502283105, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI212642053, Length=235, Percent_Identity=28.936170212766, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17536829, Length=188, Percent_Identity=33.5106382978723, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17562582, Length=225, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=347, Percent_Identity=27.3775216138329, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323961, Length=362, Percent_Identity=25.414364640884, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=362, Percent_Identity=24.8618784530387, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324195, Length=243, Percent_Identity=30.8641975308642, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581341, Length=363, Percent_Identity=29.2011019283747, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24581339, Length=363, Percent_Identity=29.2011019283747, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI221330659, Length=232, Percent_Identity=28.0172413793103, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920632, Length=232, Percent_Identity=28.0172413793103, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581345, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36285; Mature: 36285

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFILERYGKKRALRLADMPTPELRDDEVLVQVHAAGVNLLDSKIRDGEFKLILPYRLP
CCCHHHHHHCCHHEEEECCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCEEEEECEECC
LILGHDVAGVIVKVGPQVRQFKLGDEVYARPDDFRIGTFAEFVPVKEASLALKPKGLTME
EEECCCHHEEEEECCCCCEEEECCCHHCCCCCCCEECCHHHHCCCCCCCEEECCCCCCHH
EAASIPLVALTAWQALVEKANLKKGQKVFIQAGSGGVGTFAIQLAKHLGATVATTTSTTN
HHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCH
VALVKSLGADVVIDYKTQDFEDVLRDYDVVLNSQDGKTLEKSLRVLKGGGKLISISGPPD
HHHHHHCCCCEEEEECCCCHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCEEEECCCCCC
PEFGKESGAPGFVKLVMRLLSSGIRRKARSRGVSFSFLFMKANGSQLREVTRLFDAGVIR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHH
PVMDRVFPFESTNEAMAYVEAGRAKGKVVVKIK
HHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MKAFILERYGKKRALRLADMPTPELRDDEVLVQVHAAGVNLLDSKIRDGEFKLILPYRLP
CCCHHHHHHCCHHEEEECCCCCCCCCCCEEEEEEEECCCHHHHHCCCCCCEEEEECEECC
LILGHDVAGVIVKVGPQVRQFKLGDEVYARPDDFRIGTFAEFVPVKEASLALKPKGLTME
EEECCCHHEEEEECCCCCEEEECCCHHCCCCCCCEECCHHHHCCCCCCCEEECCCCCCHH
EAASIPLVALTAWQALVEKANLKKGQKVFIQAGSGGVGTFAIQLAKHLGATVATTTSTTN
HHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCH
VALVKSLGADVVIDYKTQDFEDVLRDYDVVLNSQDGKTLEKSLRVLKGGGKLISISGPPD
HHHHHHCCCCEEEEECCCCHHHHHHHHHEEEECCCCHHHHHHHHHHCCCCEEEECCCCCC
PEFGKESGAPGFVKLVMRLLSSGIRRKARSRGVSFSFLFMKANGSQLREVTRLFDAGVIR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHH
PVMDRVFPFESTNEAMAYVEAGRAKGKVVVKIK
HHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]