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The map label for this gene is pdhB [H]

Identifier: 89898337

GI number: 89898337

Start: 622002

End: 622988

Strand: Direct

Name: pdhB [H]

Synonym: CF0530

Alternate gene names: 89898337

Gene position: 622002-622988 (Clockwise)

Preceding gene: 89898336

Following gene: 89898338

Centisome position: 53.33

GC content: 40.43

Gene sequence:

>987_bases
ATGCCTAAGCACGTTACATTGGAAATCCGAGAAGCTATTAGAGAAGCCATAGACGAAGAGATGGCCAGGGATCCTAATGT
ATGCATACTGGGAGAAGAAGTCGCCGAGTACAACGGTGCCTATAAAGTAACAAAAGGTCTTTTAGACAAATGGTCGTCAT
CACGGGTTATTGACACACCCATTAGCGAAGCTGCATTTGCAGGAATTGGCATTGGAGCTGCGTTAACAGGTCTTCGTCCA
ATTATAGAATTCATGAGCTGGAACTTCTCTTTAGTTGCTGCGGATCAAATTATCTCGCACGCCGCAAAAATGCATTACAT
GACTGGTGGAAAATTTTCTGTTCCTATTGTATTTCGCGGTCCCAACGGAGCTGCGGCCCAAGTATCTTGTCAACACTCTC
ACTGTGTAGAAGCTCTCTACGCTAACATCCCCGGCTTAATTGTTGTTTCCCCCTCTAATCCTTTCGATGCTAAGGGATTA
TTAAAATCTGCAATTAGGAATGATAATCCCGTACTCTTCTTAGAAAATGAGTTGGAGTACAATCTCAAGGGTGAAGTCCC
CATTGAAGAGTACCTGGTTCCTATTGGCAAATCTCGTATTATCGAGGAAGGTAAAGACCTAACGATCATTACTTATGGTC
GTATGGTTTCTATTGTAAAACAAGCTGTAAACATAGCTAAACAACGCTATGGTCTTTCTATAGAAATTCTTGATCTGCGA
ACAATTAAGCCTCTAGATATCTCTGGAATATTTTCTTCAATAAAGAAAACAGGAAACTGTATAGTTGTAGAAGAGGGTCA
CTATTTTGCAGGAATTTCAGCGGAAATCATTACTGAAATTATAGAACATGTTTTTGATTACCTAGATAGTCCTCCTTTAA
GAGTGTGTCAAAGAGAAACTCCTATGCCCTATAACAAAACTCTAGAGCAAGCAACTCTCCCGAACGTGCATCGTATTTTA
GACACTATCGAAAAAATCATGAGGTAA

Upstream 100 bases:

>100_bases
CAAGCGATGCGACAAGAGTGTAGGAGTGAGGTCGTGAAAGCGTTCACCGAAGCAAAATCAGATCCAGAACCAACGATTGC
CACATTAGAAGAAGGTGTTT

Downstream 100 bases:

>100_bases
ATTTGTGATTTCTTTATTAAAAATGCCAAAACTCTCTCCAACAATGGAAGTAGGAACAATCGTAAAATGGCATAAGAACA
ATGGCGATAAGGTAGAGTTT

Product: pyruvate dehydrogenase E1 beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPKHVTLEIREAIREAIDEEMARDPNVCILGEEVAEYNGAYKVTKGLLDKWSSSRVIDTPISEAAFAGIGIGAALTGLRP
IIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRGPNGAAAQVSCQHSHCVEALYANIPGLIVVSPSNPFDAKGL
LKSAIRNDNPVLFLENELEYNLKGEVPIEEYLVPIGKSRIIEEGKDLTIITYGRMVSIVKQAVNIAKQRYGLSIEILDLR
TIKPLDISGIFSSIKKTGNCIVVEEGHYFAGISAEIITEIIEHVFDYLDSPPLRVCQRETPMPYNKTLEQATLPNVHRIL
DTIEKIMR

Sequences:

>Translated_328_residues
MPKHVTLEIREAIREAIDEEMARDPNVCILGEEVAEYNGAYKVTKGLLDKWSSSRVIDTPISEAAFAGIGIGAALTGLRP
IIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRGPNGAAAQVSCQHSHCVEALYANIPGLIVVSPSNPFDAKGL
LKSAIRNDNPVLFLENELEYNLKGEVPIEEYLVPIGKSRIIEEGKDLTIITYGRMVSIVKQAVNIAKQRYGLSIEILDLR
TIKPLDISGIFSSIKKTGNCIVVEEGHYFAGISAEIITEIIEHVFDYLDSPPLRVCQRETPMPYNKTLEQATLPNVHRIL
DTIEKIMR
>Mature_327_residues
PKHVTLEIREAIREAIDEEMARDPNVCILGEEVAEYNGAYKVTKGLLDKWSSSRVIDTPISEAAFAGIGIGAALTGLRPI
IEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRGPNGAAAQVSCQHSHCVEALYANIPGLIVVSPSNPFDAKGLL
KSAIRNDNPVLFLENELEYNLKGEVPIEEYLVPIGKSRIIEEGKDLTIITYGRMVSIVKQAVNIAKQRYGLSIEILDLRT
IKPLDISGIFSSIKKTGNCIVVEEGHYFAGISAEIITEIIEHVFDYLDSPPLRVCQRETPMPYNKTLEQATLPNVHRILD
TIEKIMR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=331, Percent_Identity=48.3383685800604, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI291084858, Length=331, Percent_Identity=44.4108761329305, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4557353, Length=323, Percent_Identity=35.9133126934984, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI34101272, Length=323, Percent_Identity=35.9133126934984, Blast_Score=228, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI17538422, Length=327, Percent_Identity=50.4587155963303, Blast_Score=326, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI17506935, Length=321, Percent_Identity=35.202492211838, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=47.0948012232416, Blast_Score=315, Evalue=5e-87,
Organism=Drosophila melanogaster, GI21358145, Length=327, Percent_Identity=48.9296636085627, Blast_Score=325, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24650940, Length=327, Percent_Identity=48.9296636085627, Blast_Score=325, Evalue=2e-89,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=35.2941176470588, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=35.2941176470588, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=59.5505617977528, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=59.5505617977528, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36306; Mature: 36175

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHVTLEIREAIREAIDEEMARDPNVCILGEEVAEYNGAYKVTKGLLDKWSSSRVIDTP
CCCCEEHHHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCCHHHHHHHHHHCCCCCEEECC
ISEAAFAGIGIGAALTGLRPIIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRG
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHEECCCCEEEEEEEEC
PNGAAAQVSCQHSHCVEALYANIPGLIVVSPSNPFDAKGLLKSAIRNDNPVLFLENELEY
CCCCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
NLKGEVPIEEYLVPIGKSRIIEEGKDLTIITYGRMVSIVKQAVNIAKQRYGLSIEILDLR
CCCCCCCHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TIKPLDISGIFSSIKKTGNCIVVEEGHYFAGISAEIITEIIEHVFDYLDSPPLRVCQRET
CCCCCCHHHHHHHHHHCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
PMPYNKTLEQATLPNVHRILDTIEKIMR
CCCHHHHHHHHCCCHHHHHHHHHHHHHC
>Mature Secondary Structure 
PKHVTLEIREAIREAIDEEMARDPNVCILGEEVAEYNGAYKVTKGLLDKWSSSRVIDTP
CCCEEHHHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCCHHHHHHHHHHCCCCCEEECC
ISEAAFAGIGIGAALTGLRPIIEFMSWNFSLVAADQIISHAAKMHYMTGGKFSVPIVFRG
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHEECCCCEEEEEEEEC
PNGAAAQVSCQHSHCVEALYANIPGLIVVSPSNPFDAKGLLKSAIRNDNPVLFLENELEY
CCCCEEEEECCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
NLKGEVPIEEYLVPIGKSRIIEEGKDLTIITYGRMVSIVKQAVNIAKQRYGLSIEILDLR
CCCCCCCHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
TIKPLDISGIFSSIKKTGNCIVVEEGHYFAGISAEIITEIIEHVFDYLDSPPLRVCQRET
CCCCCCHHHHHHHHHHCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
PMPYNKTLEQATLPNVHRILDTIEKIMR
CCCHHHHHHHHCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]