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The map label for this gene is pdhC [H]

Identifier: 89898338

GI number: 89898338

Start: 622993

End: 624279

Strand: Direct

Name: pdhC [H]

Synonym: CF0531

Alternate gene names: 89898338

Gene position: 622993-624279 (Clockwise)

Preceding gene: 89898337

Following gene: 89898340

Centisome position: 53.42

GC content: 38.93

Gene sequence:

>1287_bases
GTGATTTCTTTATTAAAAATGCCAAAACTCTCTCCAACAATGGAAGTAGGAACAATCGTAAAATGGCATAAGAACAATGG
CGATAAGGTAGAGTTTGGTGATGTTCTCGTAGAAATATCCACAGACAAAGCTGTGTTAGAACATACGGCTACTGAAGATG
GTTGGTTCCGAGAGAGCCTTGTTAAAGAAGGAACAAAAGTACAAATAGGCATACCTATAGCTGTGATATCTTCCGAAAAA
GATGAATCTTTTAATTTAGAGGAACTACTTCCTAAATCTCCTGAACCTCAACCTTCTGCAGAAAATATTCAACAAGTAGA
AGAAGTTGCATCAAGCGCGCCTCGTTGCGAGTCACCAGCTATAGCAGTTTACGGGTTTAAACCCGAACCCCCTCTTTCCG
AACCCCTATGTTTAAAACAAGATTCTTCAAAATCTCCAATTTCTCCTCTAGCTAAACGCTTAGCCAAGGAAAAGAATCTT
GATATCTCAGGAATCAAAGGTAGCGGCCCAGGCGGACGTATAGTTGAAAAAGATCTTGCGAAAGCACCGCCTAAAGGTAT
CGCCGGTTTTGGCTATCCAGAAGCTCCTGAGGTGCATCCAGGATCATATCATGAAGAATCTCTCTCCCCAGTTCGAGAAA
TCATTTCTCAAAGATTACAAGCAGCTAAAACTTTTATTCCCCATTTTTATGTACGACAAAAAGTATACGCCTCACCATTA
TTAGCTTTGCTTAAAGAGCTGCAAATCCAAGGAATAAAGCTTTCTATTAATGATTGTATTGTTCGTGCATGTGCCCTAGC
TTTAAAAGAATTTCCAGAAGTCAATTCTGGATTTAATAGCGTCGACAATAAAATTGTACGTTTTGAAACTATAGATATTT
CTATTGCTGTAGCTATTCCCGACGGTGTAATTACTCCAATTGTACGATGTGCTGACCGTAAGAACATTGGAATGATTTCC
GCAGAAATAAAAAGCTTAGCATCCAAAGCTAAGTCTCAATCTTTAAAAAAAGAAGAGTACACGGGAGGATCTTTCTGTGT
TTCTAATCTAGGCATGACAGGAATTACAGAATTCACAGCAATTATTAATCCTCCTCAGGCAGCTATTCTTGCTGTAGGTA
GTGTACAAGAAGAGCCTACAGTTATTAATGGAGAAATTGTCATCGGCTCTACATGCATGCTGACATTATCCATAGATCAC
CGTGTAGTTGATGGATATCCTGCAGCAATGTTTATGAAACGGTTGCAGAAAATCTTAGAAGCACCTTCCGTTCTTTTATT
AAATTAA

Upstream 100 bases:

>100_bases
AGAGAAACTCCTATGCCCTATAACAAAACTCTAGAGCAAGCAACTCTCCCGAACGTGCATCGTATTTTAGACACTATCGA
AAAAATCATGAGGTAAATTT

Downstream 100 bases:

>100_bases
AAAACACCTCTGCAACTATGCACAATGAGAAAAAGCGTTTCTAACAAGGAAAGCTCTCGCAAAAAGAAGCGCCTTTTTCT
TAAAATTTGTCATTTGTAGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MISLLKMPKLSPTMEVGTIVKWHKNNGDKVEFGDVLVEISTDKAVLEHTATEDGWFRESLVKEGTKVQIGIPIAVISSEK
DESFNLEELLPKSPEPQPSAENIQQVEEVASSAPRCESPAIAVYGFKPEPPLSEPLCLKQDSSKSPISPLAKRLAKEKNL
DISGIKGSGPGGRIVEKDLAKAPPKGIAGFGYPEAPEVHPGSYHEESLSPVREIISQRLQAAKTFIPHFYVRQKVYASPL
LALLKELQIQGIKLSINDCIVRACALALKEFPEVNSGFNSVDNKIVRFETIDISIAVAIPDGVITPIVRCADRKNIGMIS
AEIKSLASKAKSQSLKKEEYTGGSFCVSNLGMTGITEFTAIINPPQAAILAVGSVQEEPTVINGEIVIGSTCMLTLSIDH
RVVDGYPAAMFMKRLQKILEAPSVLLLN

Sequences:

>Translated_428_residues
MISLLKMPKLSPTMEVGTIVKWHKNNGDKVEFGDVLVEISTDKAVLEHTATEDGWFRESLVKEGTKVQIGIPIAVISSEK
DESFNLEELLPKSPEPQPSAENIQQVEEVASSAPRCESPAIAVYGFKPEPPLSEPLCLKQDSSKSPISPLAKRLAKEKNL
DISGIKGSGPGGRIVEKDLAKAPPKGIAGFGYPEAPEVHPGSYHEESLSPVREIISQRLQAAKTFIPHFYVRQKVYASPL
LALLKELQIQGIKLSINDCIVRACALALKEFPEVNSGFNSVDNKIVRFETIDISIAVAIPDGVITPIVRCADRKNIGMIS
AEIKSLASKAKSQSLKKEEYTGGSFCVSNLGMTGITEFTAIINPPQAAILAVGSVQEEPTVINGEIVIGSTCMLTLSIDH
RVVDGYPAAMFMKRLQKILEAPSVLLLN
>Mature_428_residues
MISLLKMPKLSPTMEVGTIVKWHKNNGDKVEFGDVLVEISTDKAVLEHTATEDGWFRESLVKEGTKVQIGIPIAVISSEK
DESFNLEELLPKSPEPQPSAENIQQVEEVASSAPRCESPAIAVYGFKPEPPLSEPLCLKQDSSKSPISPLAKRLAKEKNL
DISGIKGSGPGGRIVEKDLAKAPPKGIAGFGYPEAPEVHPGSYHEESLSPVREIISQRLQAAKTFIPHFYVRQKVYASPL
LALLKELQIQGIKLSINDCIVRACALALKEFPEVNSGFNSVDNKIVRFETIDISIAVAIPDGVITPIVRCADRKNIGMIS
AEIKSLASKAKSQSLKKEEYTGGSFCVSNLGMTGITEFTAIINPPQAAILAVGSVQEEPTVINGEIVIGSTCMLTLSIDH
RVVDGYPAAMFMKRLQKILEAPSVLLLN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=35.9447004608295, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=33.6225596529284, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=33.6225596529284, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=34.051724137931, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=25.2272727272727, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=43.6708860759494, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786946, Length=433, Percent_Identity=32.7944572748268, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786305, Length=293, Percent_Identity=28.6689419795222, Blast_Score=125, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560088, Length=434, Percent_Identity=38.2488479262673, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=38.0530973451327, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=429, Percent_Identity=29.6037296037296, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=26.6355140186916, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=466, Percent_Identity=33.4763948497854, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=27.5229357798165, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI20129315, Length=289, Percent_Identity=38.0622837370242, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24582497, Length=289, Percent_Identity=38.0622837370242, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24645909, Length=176, Percent_Identity=35.2272727272727, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18859875, Length=311, Percent_Identity=28.2958199356913, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46345; Mature: 46345

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLLKMPKLSPTMEVGTIVKWHKNNGDKVEFGDVLVEISTDKAVLEHTATEDGWFRESL
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECEEEEEECCCHHHHHHCCCCCCHHHHHH
VKEGTKVQIGIPIAVISSEKDESFNLEELLPKSPEPQPSAENIQQVEEVASSAPRCESPA
HCCCCEEEEECEEEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCE
IAVYGFKPEPPLSEPLCLKQDSSKSPISPLAKRLAKEKNLDISGIKGSGPGGRIVEKDLA
EEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHH
KAPPKGIAGFGYPEAPEVHPGSYHEESLSPVREIISQRLQAAKTFIPHFYVRQKVYASPL
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLKELQIQGIKLSINDCIVRACALALKEFPEVNSGFNSVDNKIVRFETIDISIAVAIP
HHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEEEEEEEECC
DGVITPIVRCADRKNIGMISAEIKSLASKAKSQSLKKEEYTGGSFCVSNLGMTGITEFTA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHH
IINPPQAAILAVGSVQEEPTVINGEIVIGSTCMLTLSIDHRVVDGYPAAMFMKRLQKILE
CCCCCHHEEEEECCCCCCCEEEECEEEECCEEEEEEEECCEEECCCCHHHHHHHHHHHHC
APSVLLLN
CCCEEEEC
>Mature Secondary Structure
MISLLKMPKLSPTMEVGTIVKWHKNNGDKVEFGDVLVEISTDKAVLEHTATEDGWFRESL
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECEEEEEECCCHHHHHHCCCCCCHHHHHH
VKEGTKVQIGIPIAVISSEKDESFNLEELLPKSPEPQPSAENIQQVEEVASSAPRCESPA
HCCCCEEEEECEEEEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCE
IAVYGFKPEPPLSEPLCLKQDSSKSPISPLAKRLAKEKNLDISGIKGSGPGGRIVEKDLA
EEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHH
KAPPKGIAGFGYPEAPEVHPGSYHEESLSPVREIISQRLQAAKTFIPHFYVRQKVYASPL
HCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLKELQIQGIKLSINDCIVRACALALKEFPEVNSGFNSVDNKIVRFETIDISIAVAIP
HHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEEEEEEEECC
DGVITPIVRCADRKNIGMISAEIKSLASKAKSQSLKKEEYTGGSFCVSNLGMTGITEFTA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHH
IINPPQAAILAVGSVQEEPTVINGEIVIGSTCMLTLSIDHRVVDGYPAAMFMKRLQKILE
CCCCCHHEEEEECCCCCCCEEEECEEEECCEEEEEEEECCEEECCCCHHHHHHHHHHHHC
APSVLLLN
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]