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The map label for this gene is lysC [H]

Identifier: 89898112

GI number: 89898112

Start: 352564

End: 353886

Strand: Direct

Name: lysC [H]

Synonym: CF0305

Alternate gene names: 89898112

Gene position: 352564-353886 (Clockwise)

Preceding gene: 89898111

Following gene: 89898113

Centisome position: 30.23

GC content: 38.25

Gene sequence:

>1323_bases
ATGCTCCCAGTAGTGTACAAATTTGGTGGGACGAGTTTAGGAACTGCAGAAAGTATCAAAAAGGTATATGATATTGTCTG
CAAAAATACACCGCGTTTTATTGTTGTCAGTGCTGTAGCGGGAATAACAGATCTATTAGATCTATTTTGTGCTGTTTCTA
GAGAATCTCGCGAACAAATCATTTTCGATATTGCGAAGAAACATAATGAAATTATCCGTGATCTACAACTCATATTTTCT
ATATCTCCCTCGATGGAGAGATTGTACAATTGTGTAGATAAAGAAGAAATTTCTCCTAGCGATCGTGCGGAGATTCTTGC
TTTAGGTGAGGACATATCTGCTTCTTTATTTCAAGCCTTCTGTCGTAGTAATAACTTCTCTCTAGAGTTTTTAGAAGCAA
GAACTGTCATACTAACGGATGATGAATATGATCGTGCAACTCCTGATGTTTTTCGTATGCACTCAAACTGGCAGACTCTT
AATTTGCAAAGAGATGTTTGCTACATCACACAAGGATTTATAGGGGCTAATTCTTCTGGAAAGACGACTTTATTAGGTCG
AGGAGGAAGTGATTTCTCCGGAGCTTTAATTGCAGAAATGAGCAATGCTAGGGAAGTACGTATTTATACCGATGTTAACG
GTATTTACACTATGGATCCTAGGATTATTAAAGATGCTCAACTCATTCCCGAGTTGAGCTTTGAGGAAATGCAAAATCTT
GCAACATTTGGCGCGAAGATTCTCCATCCTCCTATGCTTTCTCCTTGCGTACGCGCAGGAATTCCTATTTTTGTAACTTC
TACGTTTGATGTCTCTAAAGGGGGGACGTGGATCTATGCTATGGATAAAATTGTAGGCTATGAACCACGTGTAAAAGCTT
TATCTCTACGTCTGCATCAACGTTTGTGGTCGGTAGACTGTGGTAATTTGGGAGCTGCAAGCTTAGATAAGGTGTTATCT
ATATTAGAAAGTTATCGTGTTTTTCCTGGATTGATAACATCTCAAGATAGTGCGGTATCTTTTACAACAGATGATGATGA
TGTTTCCGACAATGTTATTAGAGATCTTTATGGTCAGCTATCTAGCATAGGTTCAGTTCATATGCTTTATGACCTAGCCT
TAATTACTATGATCGGTTCTGGATTGGCTTCAACAAAAGTCGTAACAACAGTTACGGAGAAATTACGCAACTATCTTTCT
CCAGTTTTTTGCTGTTGCCAAAGTTGCATGGCTTTAAACTTGGTGGTTCCTGAAAATCTCGCAGGAAGTATTGTAGAACA
ACTCCATAATGATTATGTTAAGCAAAAGTTTTCTGTAGTATAA

Upstream 100 bases:

>100_bases
TAAAGATGAATGTATTGGTACATAATCTTGTTCGAGGGGCTGCGGGCACTTTGTTGAAAAATATGCACAACTACTGTTTT
CAGAATTCTGGAGAATATGC

Downstream 100 bases:

>100_bases
TAGCTGATCATTATGAAATTACTTACAGCGAGTGTCACCCCATTCCTTCCTAATTATGATATAGATTTTCTAAGTGTTGA
AAAGTTGCTGCATTCTCAAG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLPVVYKFGGTSLGTAESIKKVYDIVCKNTPRFIVVSAVAGITDLLDLFCAVSRESREQIIFDIAKKHNEIIRDLQLIFS
ISPSMERLYNCVDKEEISPSDRAEILALGEDISASLFQAFCRSNNFSLEFLEARTVILTDDEYDRATPDVFRMHSNWQTL
NLQRDVCYITQGFIGANSSGKTTLLGRGGSDFSGALIAEMSNAREVRIYTDVNGIYTMDPRIIKDAQLIPELSFEEMQNL
ATFGAKILHPPMLSPCVRAGIPIFVTSTFDVSKGGTWIYAMDKIVGYEPRVKALSLRLHQRLWSVDCGNLGAASLDKVLS
ILESYRVFPGLITSQDSAVSFTTDDDDVSDNVIRDLYGQLSSIGSVHMLYDLALITMIGSGLASTKVVTTVTEKLRNYLS
PVFCCCQSCMALNLVVPENLAGSIVEQLHNDYVKQKFSVV

Sequences:

>Translated_440_residues
MLPVVYKFGGTSLGTAESIKKVYDIVCKNTPRFIVVSAVAGITDLLDLFCAVSRESREQIIFDIAKKHNEIIRDLQLIFS
ISPSMERLYNCVDKEEISPSDRAEILALGEDISASLFQAFCRSNNFSLEFLEARTVILTDDEYDRATPDVFRMHSNWQTL
NLQRDVCYITQGFIGANSSGKTTLLGRGGSDFSGALIAEMSNAREVRIYTDVNGIYTMDPRIIKDAQLIPELSFEEMQNL
ATFGAKILHPPMLSPCVRAGIPIFVTSTFDVSKGGTWIYAMDKIVGYEPRVKALSLRLHQRLWSVDCGNLGAASLDKVLS
ILESYRVFPGLITSQDSAVSFTTDDDDVSDNVIRDLYGQLSSIGSVHMLYDLALITMIGSGLASTKVVTTVTEKLRNYLS
PVFCCCQSCMALNLVVPENLAGSIVEQLHNDYVKQKFSVV
>Mature_440_residues
MLPVVYKFGGTSLGTAESIKKVYDIVCKNTPRFIVVSAVAGITDLLDLFCAVSRESREQIIFDIAKKHNEIIRDLQLIFS
ISPSMERLYNCVDKEEISPSDRAEILALGEDISASLFQAFCRSNNFSLEFLEARTVILTDDEYDRATPDVFRMHSNWQTL
NLQRDVCYITQGFIGANSSGKTTLLGRGGSDFSGALIAEMSNAREVRIYTDVNGIYTMDPRIIKDAQLIPELSFEEMQNL
ATFGAKILHPPMLSPCVRAGIPIFVTSTFDVSKGGTWIYAMDKIVGYEPRVKALSLRLHQRLWSVDCGNLGAASLDKVLS
ILESYRVFPGLITSQDSAVSFTTDDDDVSDNVIRDLYGQLSSIGSVHMLYDLALITMIGSGLASTKVVTTVTEKLRNYLS
PVFCCCQSCMALNLVVPENLAGSIVEQLHNDYVKQKFSVV

Specific function: Lysine biosynthesis; first step. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790455, Length=460, Percent_Identity=34.1304347826087, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786183, Length=422, Percent_Identity=26.0663507109005, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790376, Length=286, Percent_Identity=28.3216783216783, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6320893, Length=458, Percent_Identity=23.3624454148472, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 48712; Mature: 48712

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVVYKFGGTSLGTAESIKKVYDIVCKNTPRFIVVSAVAGITDLLDLFCAVSRESREQI
CCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHH
IFDIAKKHNEIIRDLQLIFSISPSMERLYNCVDKEEISPSDRAEILALGEDISASLFQAF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHCCCCCCCCEEEEECCCHHHHHHHHH
CRSNNFSLEFLEARTVILTDDEYDRATPDVFRMHSNWQTLNLQRDVCYITQGFIGANSSG
HCCCCCCEEEEECEEEEEECCCCCCCCCHHHHCCCCCEEEECCCCCCEEECCCCCCCCCC
KTTLLGRGGSDFSGALIAEMSNAREVRIYTDVNGIYTMDPRIIKDAQLIPELSFEEMQNL
CEEEEECCCCCCCCHHHHCCCCCCEEEEEEECCCEEECCCHHHHHHHHCCCCCHHHHHHH
ATFGAKILHPPMLSPCVRAGIPIFVTSTFDVSKGGTWIYAMDKIVGYEPRVKALSLRLHQ
HHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEEEHHHHCCCCCHHHHHHHHHH
RLWSVDCGNLGAASLDKVLSILESYRVFPGLITSQDSAVSFTTDDDDVSDNVIRDLYGQL
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHH
SSIGSVHMLYDLALITMIGSGLASTKVVTTVTEKLRNYLSPVFCCCQSCMALNLVVPENL
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
AGSIVEQLHNDYVKQKFSVV
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPVVYKFGGTSLGTAESIKKVYDIVCKNTPRFIVVSAVAGITDLLDLFCAVSRESREQI
CCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCHHHHHH
IFDIAKKHNEIIRDLQLIFSISPSMERLYNCVDKEEISPSDRAEILALGEDISASLFQAF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHCCCCCCCCEEEEECCCHHHHHHHHH
CRSNNFSLEFLEARTVILTDDEYDRATPDVFRMHSNWQTLNLQRDVCYITQGFIGANSSG
HCCCCCCEEEEECEEEEEECCCCCCCCCHHHHCCCCCEEEECCCCCCEEECCCCCCCCCC
KTTLLGRGGSDFSGALIAEMSNAREVRIYTDVNGIYTMDPRIIKDAQLIPELSFEEMQNL
CEEEEECCCCCCCCHHHHCCCCCCEEEEEEECCCEEECCCHHHHHHHHCCCCCHHHHHHH
ATFGAKILHPPMLSPCVRAGIPIFVTSTFDVSKGGTWIYAMDKIVGYEPRVKALSLRLHQ
HHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCCCEEEEEEHHHHCCCCCHHHHHHHHHH
RLWSVDCGNLGAASLDKVLSILESYRVFPGLITSQDSAVSFTTDDDDVSDNVIRDLYGQL
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHH
SSIGSVHMLYDLALITMIGSGLASTKVVTTVTEKLRNYLSPVFCCCQSCMALNLVVPENL
HHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHH
AGSIVEQLHNDYVKQKFSVV
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]