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The map label for this gene is dapA

Identifier: 89898113

GI number: 89898113

Start: 353899

End: 354771

Strand: Direct

Name: dapA

Synonym: CF0306

Alternate gene names: 89898113

Gene position: 353899-354771 (Clockwise)

Preceding gene: 89898112

Following gene: 89898114

Centisome position: 30.35

GC content: 38.6

Gene sequence:

>873_bases
ATGAAATTACTTACAGCGAGTGTCACCCCATTCCTTCCTAATTATGATATAGATTTTCTAAGTGTTGAAAAGTTGCTGCA
TTCTCAAGAAAAAGAAGGCAATGGTGTTGTTCTACTTGGAAGCACAGGAGAAAGCTTAGCGTTAACAGTACAAGAAAAGG
AAAAACTAGTTGCCTATGCCTGCTCTTTGGATCTCAAGATTCCTATAATTGTTGGTGTTCCAGGAACCTCATTACATGAA
GCTTCTGCATGGGTGAGTTTATGTCAGTCGTATCCTGTTGATGGTTTTTTAATCACTAGCCCTATTTATACTAAACCCGG
GATTCAAGGCCAGATTTTGTGGTTTGAATCCATCTTAAATATTACGAATAAACCTGCGATTCTTTATAACATCCCCTCTA
GAGCTGGATCTCCGATATATTTAGAAACCGTACGCGCTTTGTCCGGCCATCCTTTTTTCTACGGTATCAAAGATTCAGGA
GGCTCTATAGACAGATGTCGGGAATATACACAAGTATGTCCTAATCTCATTATCTATTGTGGAGATGATGGTCTATGGCC
ACAAATGCATCAGTGCGGAGCTCGTGGATTAATTTCTGTTTTATCTAATAGCTGGCCAAAAGAAGCTCATAACTATGTAG
AAGATCCTTTTAATAAGAATAATTCACTGTTATGGTGCGAGCTCGTTAGCTGGATCAATCAAACAACCAATCCTATATCT
ATTAAAGCGATGTTAGCCTACAAGCAGGATATCGCATACGATATTTTGCGTTTGCCTTTGTCTATTAAGGATTTGCAGAA
TAAAAAAGTCTTGCCTGTGCTTGTGGAAAAAATGTCACAATGGTCACAAATATGTGAATGCGTGTTCACATAG

Upstream 100 bases:

>100_bases
AAACTTGGTGGTTCCTGAAAATCTCGCAGGAAGTATTGTAGAACAACTCCATAATGATTATGTTAAGCAAAAGTTTTCTG
TAGTATAATAGCTGATCATT

Downstream 100 bases:

>100_bases
TTTTCAAAGAGAAAATCATATATTTTCAATAGTTTGCTCTTTAAGTTTTTTCGTCCTTTCTTGTTGATTTTAAATAATTT
AGATTGCATCTTTCCTTGGC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MKLLTASVTPFLPNYDIDFLSVEKLLHSQEKEGNGVVLLGSTGESLALTVQEKEKLVAYACSLDLKIPIIVGVPGTSLHE
ASAWVSLCQSYPVDGFLITSPIYTKPGIQGQILWFESILNITNKPAILYNIPSRAGSPIYLETVRALSGHPFFYGIKDSG
GSIDRCREYTQVCPNLIIYCGDDGLWPQMHQCGARGLISVLSNSWPKEAHNYVEDPFNKNNSLLWCELVSWINQTTNPIS
IKAMLAYKQDIAYDILRLPLSIKDLQNKKVLPVLVEKMSQWSQICECVFT

Sequences:

>Translated_290_residues
MKLLTASVTPFLPNYDIDFLSVEKLLHSQEKEGNGVVLLGSTGESLALTVQEKEKLVAYACSLDLKIPIIVGVPGTSLHE
ASAWVSLCQSYPVDGFLITSPIYTKPGIQGQILWFESILNITNKPAILYNIPSRAGSPIYLETVRALSGHPFFYGIKDSG
GSIDRCREYTQVCPNLIIYCGDDGLWPQMHQCGARGLISVLSNSWPKEAHNYVEDPFNKNNSLLWCELVSWINQTTNPIS
IKAMLAYKQDIAYDILRLPLSIKDLQNKKVLPVLVEKMSQWSQICECVFT
>Mature_290_residues
MKLLTASVTPFLPNYDIDFLSVEKLLHSQEKEGNGVVLLGSTGESLALTVQEKEKLVAYACSLDLKIPIIVGVPGTSLHE
ASAWVSLCQSYPVDGFLITSPIYTKPGIQGQILWFESILNITNKPAILYNIPSRAGSPIYLETVRALSGHPFFYGIKDSG
GSIDRCREYTQVCPNLIIYCGDDGLWPQMHQCGARGLISVLSNSWPKEAHNYVEDPFNKNNSLLWCELVSWINQTTNPIS
IKAMLAYKQDIAYDILRLPLSIKDLQNKKVLPVLVEKMSQWSQICECVFT

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Escherichia coli, GI1788823, Length=269, Percent_Identity=28.2527881040892, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786463, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082415, Length=181, Percent_Identity=28.7292817679558, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789620, Length=204, Percent_Identity=24.0196078431373, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_CHLFF (Q255G0)

Other databases:

- EMBL:   AP006861
- RefSeq:   YP_515223.1
- ProteinModelPortal:   Q255G0
- STRING:   Q255G0
- GeneID:   3958683
- GenomeReviews:   AP006861_GR
- KEGG:   cfe:CF0306
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   LAYKQDI
- ProtClustDB:   PRK03170
- BioCyc:   CFEL264202:CF0306-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32315; Mature: 32315

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLTASVTPFLPNYDIDFLSVEKLLHSQEKEGNGVVLLGSTGESLALTVQEKEKLVAYA
CCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEHHHHCEEEEE
CSLDLKIPIIVGVPGTSLHEASAWVSLCQSYPVDGFLITSPIYTKPGIQGQILWFESILN
EECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEHHHHHHC
ITNKPAILYNIPSRAGSPIYLETVRALSGHPFFYGIKDSGGSIDRCREYTQVCPNLIIYC
CCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEE
GDDGLWPQMHQCGARGLISVLSNSWPKEAHNYVEDPFNKNNSLLWCELVSWINQTTNPIS
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCEE
IKAMLAYKQDIAYDILRLPLSIKDLQNKKVLPVLVEKMSQWSQICECVFT
EEEEEHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLTASVTPFLPNYDIDFLSVEKLLHSQEKEGNGVVLLGSTGESLALTVQEKEKLVAYA
CCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEHHHHCEEEEE
CSLDLKIPIIVGVPGTSLHEASAWVSLCQSYPVDGFLITSPIYTKPGIQGQILWFESILN
EECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEHHHHHHC
ITNKPAILYNIPSRAGSPIYLETVRALSGHPFFYGIKDSGGSIDRCREYTQVCPNLIIYC
CCCCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEE
GDDGLWPQMHQCGARGLISVLSNSWPKEAHNYVEDPFNKNNSLLWCELVSWINQTTNPIS
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCCEE
IKAMLAYKQDIAYDILRLPLSIKDLQNKKVLPVLVEKMSQWSQICECVFT
EEEEEHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA