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The map label for this gene is slt [H]

Identifier: 89255876

GI number: 89255876

Start: 440579

End: 442555

Strand: Direct

Name: slt [H]

Synonym: FTL_0466

Alternate gene names: 89255876

Gene position: 440579-442555 (Clockwise)

Preceding gene: 89255873

Following gene: 89255880

Centisome position: 23.24

GC content: 30.96

Gene sequence:

>1977_bases
GTGATTAATAAAAAGTTCATAACAAGTTGTTTATTGATTTTATCTAGTACTATTGGTTATAGTCTCACGACACAACAAGT
TGAATATTCTCAAAAAGCTATAGATGCATTAGCTAAAAAAGATTATAAATCATATTATTATTATAAATCAAAACTCAAAG
ATACTAGCATTTATCCATACTTACAATATAAAGAAATAAGTACAGATCCAGATATTTTTCAGCAAACAACAATAGATGAG
TATTTTAAACAAGACAATAACAGCTATTGGCAAAATCGCCTTAGCGATGATTTAGCGCAATATTATGCTCAAAAACAGGA
ATGGAAACTCTTTGATAAATATTATAAAGGTGACTTGAGTATATCTGGTAAGTGTTGGAGTATGCAAGCAGAATATGAAT
CAGGTGACAAAAACAAAGCATTAAATGAATATGGTCAACTTTGGCAGAATCGAGTATATATGCCTGCTGCATGTAATCCA
ATGCAAAAATATTGGGATAATTCTGACTATAAACCAAGCAGCTATCTAACAACTAAAGCATATACTCTTGCTTTTGCTAA
TAAATTTGATAATAGCCTGTGGCTATTAAATACTTATGTTAAAGATAACAAAGATTATCTTAATTATATAACGGCATGGA
AACAGGCTACTAAAGATCCACGTAAATTGGATAGCTTCATTAATAGATTTCATAACTATAATCATTTTAACAAAGTATTT
GTTGATATTTCTAGAGACCTAATAAGAAAAGATGTTGAAAGCTACGCAAAAGTATGGGATAACCTAAAAAATAAAAGATA
TCTAAGCACGAAAGTGAAGCAACAAACTATCTCAGCAATAGCAGTAAGCTTTGCAAGGTCACAATCGCCACAAGCAAAAC
AGTGGCTAAGCAAAGTAGATAAAAATTATCTTGATACAACAGCTTGGGAGTGGTTGTTAAGAGTTGATCTATATAATGAG
AACTTCAAAGATTATATACAAACATACAATCAGCTACCTAAAAATTCTCAACAAGACCAAGCATGGAGATATTGGCTAGC
ATATAGTTATCAACAGACAGGCCAAAAAGCAAAAGCTGAAGATATATTTGAGAGTTTGACTAAGACTCCTCTTGATTATT
ATTCATTCTTAGCGGCTGATAAGCTTGGTAAGCCATATAACTTTGGTAATGATGTTGCTACAGCATTAACTAATAGTGAA
ACTAAAAAACTACTAACAGAAGATACTACTGTGCAGGCTATTGACTTATATCAGATCGGTCAATATAAAGATTCAACAAG
TATCTGGCAATGGGCAATTAGAAATAAGCTTAGAGATAAACAGATTGACGAGATTAAACAACTTGCAAGATTAGCTGAGG
ATAAGCAAATGTATTATGCAGCTATATTCAATATGTCAGTTATTGGTAGTTATAATAGTATTGACATGCTCTTTCCGAAA
GCTTTTATAAATATAGTTAATCAAAATGCTCAAAAATTTGCTATTGATAAAGATCTTGTTTTATCAATTATGCGTAAAGA
ATCACTATTTGATATTAGCGCGGGTTCTTCAGCTGGAGCAAAAGGATTGATGCAGGTTACAGAACCAACTGCAAAATTTA
TAGCTCAAAAATATAAATTATCTCTAGTTGGTGATAACTCTCAAGGTATGACTAGTCAGATATTTATCCCAGAGAATAAT
ATTAAACTTGGAACAGCTAATTTATATTTCCTAGAGAAGCTTTTTGATAAGAATCCTGTGTTAGGAATAGCAGCTTATAA
TGCTGGTCCTGGTAATGTTGCTAAGTGGCTAAATGAAAATGAGGTTCCAGCAGCAATTTGGATAGAGAATATTCCATTTG
GTGAAACACGTCATTATGTAAGAAAAGTCCTAATGTATATGATTGTATATAATAATTTTGTGTTTAAAGATAAAAAAGAT
CATATTAGTAATTTCTTGGGTTATAAGATATCTGATAAGCAAAGTTTTAGAAAATAA

Upstream 100 bases:

>100_bases
AAATTATGCTTATTAAGCTTGAAGCATGATAATTATTAGTTTTTTAATATTAATAATGTTAGTATTTTTAGTAAAATAAT
TTTCTAATTCTGAAATCATT

Downstream 100 bases:

>100_bases
TATTCAAATTATTTTAAAAAGAATATTGTAAACCTGATGTGACAAAATTTTGTGAGTTTCCTGAAAATGAAATACTATAT
GTATAAGTTACTTTAAGGCT

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MINKKFITSCLLILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPDIFQQTTIDE
YFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNP
MQKYWDNSDYKPSSYLTTKAYTLAFANKFDNSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFNKVF
VDISRDLIRKDVESYAKVWDNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEWLLRVDLYNE
NFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSE
TKKLLTEDTTVQAIDLYQIGQYKDSTSIWQWAIRNKLRDKQIDEIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPK
AFINIVNQNAQKFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMTSQIFIPENN
IKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKD
HISNFLGYKISDKQSFRK

Sequences:

>Translated_658_residues
MINKKFITSCLLILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPDIFQQTTIDE
YFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNP
MQKYWDNSDYKPSSYLTTKAYTLAFANKFDNSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFNKVF
VDISRDLIRKDVESYAKVWDNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEWLLRVDLYNE
NFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSE
TKKLLTEDTTVQAIDLYQIGQYKDSTSIWQWAIRNKLRDKQIDEIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPK
AFINIVNQNAQKFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMTSQIFIPENN
IKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKD
HISNFLGYKISDKQSFRK
>Mature_658_residues
MINKKFITSCLLILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPYLQYKEISTDPDIFQQTTIDE
YFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLSISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNP
MQKYWDNSDYKPSSYLTTKAYTLAFANKFDNSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFNKVF
VDISRDLIRKDVESYAKVWDNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVDKNYLDTTAWEWLLRVDLYNE
NFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAEDIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSE
TKKLLTEDTTVQAIDLYQIGQYKDSTSIWQWAIRNKLRDKQIDEIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPK
AFINIVNQNAQKFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKLSLVGDNSQGMTSQIFIPENN
IKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNENEVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKD
HISNFLGYKISDKQSFRK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=639, Percent_Identity=24.5696400625978, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082191, Length=165, Percent_Identity=31.5151515151515, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76932; Mature: 76932

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKKFITSCLLILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPY
CCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LQYKEISTDPDIFQQTTIDEYFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLS
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYKPSSYLTTKA
ECCCEEEEECCCCCCCCHHHHHHHHHHHHCCEECCCCCCHHHHHCCCCCCCCCCCEEHHH
YTLAFANKFDNSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFNKVF
HHHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
VDISRDLIRKDVESYAKVWDNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KNYLDTTAWEWLLRVDLYNENFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAE
HHHHHHHHHHHHHEEHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
DIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTVQAIDLYQIG
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCCEEEEEHHCCC
QYKDSTSIWQWAIRNKLRDKQIDEIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPK
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AFINIVNQNAQKFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKL
HHHHHHCCCCHHHCCCHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHEE
SLVGDNSQGMTSQIFIPENNIKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNEN
EEECCCCCCCEEEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCC
EVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKISDKQSFRK
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MINKKFITSCLLILSSTIGYSLTTQQVEYSQKAIDALAKKDYKSYYYYKSKLKDTSIYPY
CCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LQYKEISTDPDIFQQTTIDEYFKQDNNSYWQNRLSDDLAQYYAQKQEWKLFDKYYKGDLS
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ISGKCWSMQAEYESGDKNKALNEYGQLWQNRVYMPAACNPMQKYWDNSDYKPSSYLTTKA
ECCCEEEEECCCCCCCCHHHHHHHHHHHHCCEECCCCCCHHHHHCCCCCCCCCCCEEHHH
YTLAFANKFDNSLWLLNTYVKDNKDYLNYITAWKQATKDPRKLDSFINRFHNYNHFNKVF
HHHHHHHHCCCCEEEEEEHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
VDISRDLIRKDVESYAKVWDNLKNKRYLSTKVKQQTISAIAVSFARSQSPQAKQWLSKVD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KNYLDTTAWEWLLRVDLYNENFKDYIQTYNQLPKNSQQDQAWRYWLAYSYQQTGQKAKAE
HHHHHHHHHHHHHEEHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
DIFESLTKTPLDYYSFLAADKLGKPYNFGNDVATALTNSETKKLLTEDTTVQAIDLYQIG
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHCCCCEEEEEHHCCC
QYKDSTSIWQWAIRNKLRDKQIDEIKQLARLAEDKQMYYAAIFNMSVIGSYNSIDMLFPK
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
AFINIVNQNAQKFAIDKDLVLSIMRKESLFDISAGSSAGAKGLMQVTEPTAKFIAQKYKL
HHHHHHCCCCHHHCCCHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHEE
SLVGDNSQGMTSQIFIPENNIKLGTANLYFLEKLFDKNPVLGIAAYNAGPGNVAKWLNEN
EEECCCCCCCEEEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCC
EVPAAIWIENIPFGETRHYVRKVLMYMIVYNNFVFKDKKDHISNFLGYKISDKQSFRK
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]