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The map label for this gene is gdh [H]

Identifier: 89255702

GI number: 89255702

Start: 254991

End: 256340

Strand: Reverse

Name: gdh [H]

Synonym: FTL_0269

Alternate gene names: 89255702

Gene position: 256340-254991 (Counterclockwise)

Preceding gene: 89255707

Following gene: 89255657

Centisome position: 13.52

GC content: 38.15

Gene sequence:

>1350_bases
ATGAATGCTCAGAAGTACATTGATAGCGTAATTGCTCAAGTAGAAAAAAGAGATGGTCATGAAAAAGAATTCATCCAAGC
TGTAAAAGAAGTATTTTCTACACTTAAACCAGCTCTAGAACATAATCCTAAATATATCGAAGAAAATATTCTAGCACGTA
TGGTTGAGCCTGAAAGAGGTATTTCTTTTAGAGTACCATGGGTTGATAAGGATGGTAATATCCAAGTAAACCGTGGTTAC
AGATATCAGTTTAATGGCGCTATCGGTCCTTTTAAAGGCGGGATTAGATTCCACCCAAGTGTATACTCTGGTATTATCAA
ATTCTTAGGCTTCGAGCAAGTTTTCAAAAACAGCTTAACTACTCTACCTATGGGTGGTGGTAAAGGTGGTGCTGACTTTG
ACCCTAAAGGCAAAACTGATGCTGAAATCATGAACTTCTGCCAAAGCTTTATGATGGAATTACAACGTCATATTGGTCCA
GATATCGATGTCCCAGCTGGTGATATTGGTGTTGGTGGTAAAGAAATCGGCTATATGTATGGACAATACAGAAGAATTCG
TGCATGTTTTGAGAATGGTGTATTAACTGGTAAATCGCTAGAGTCAGGCGGTAGCTTAATCCGTCCTGAGGCTACAGGTT
ATGGTGCAGTATTCTTCTTAGATGAAATGCTAAAGCATGATGGTGAAACTTTACAGGGTAAAACTGTAGTAACATCTGGT
TATGGTAACGTTGCTTGGGGTGTATGTAAGAAAGTTGCTCAATTAGGCGGTAAAGTTGTTACAATATCTGGTTCAAAAGG
TTTTGTTCACGATCCTGAAGGTATTACAACTGATGAGAAAATTGAATTCTTATTAAAAATCCGTAACGGTGAAAAAACAA
TGCAAGACTACGCTAAAGAATTCAATGCAAGCTGGCACGCAGGTCAAAAACCTTGGGGTATAAAAGCAGATATCGCTATC
CCTGCAGCTACTCAAAACGAGATAGATTTTGAAGATGCTCAAAAACTTATTGATTCTGGTGTTAAGTATGTTGTTGAAGC
ATCTAACATGCCAACAACTAATGAAGCAATTGAATTCCTTATGAAGAAAGGTATAATTCTAGCTCCAGGTAAAGCTGCTA
ACGCTGGTGGTGTTGCAGTTTCTGGTCTAGAAATGAGCCAAAACTCAGCTAGACTATCTTGGTCAGCTGAAGAAGTTGAA
TCTAAACTTCAACAAATCATGGCTAACATTTTCCATGCTTGTAAGTTTGCTAGTAGTAAATACAACCTTGGCTACAACCT
AGTTGCTGGTGCAAACTTAGCTGGTTTTGAAAAAGTAGCAGAAGCTATGATTCAACAAGGTAGATACTAA

Upstream 100 bases:

>100_bases
TTATTCATAAGTTTCTTTATAATAACTGATATAAAGAGACCGGTTTAACACGTATGAAAAAATATTCTAATAAATTTCAA
ATCATAAAGGAGATGCATAA

Downstream 100 bases:

>100_bases
TTCTTTATTTTTCTTAAAATTTCTTTTTTATAATTTTTTCAAACAACTGCTATACTTTGTTACACTTAGTACACAAAGTA
TCTTTGATTTTATAATGACT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNAQKYIDSVIAQVEKRDGHEKEFIQAVKEVFSTLKPALEHNPKYIEENILARMVEPERGISFRVPWVDKDGNIQVNRGY
RYQFNGAIGPFKGGIRFHPSVYSGIIKFLGFEQVFKNSLTTLPMGGGKGGADFDPKGKTDAEIMNFCQSFMMELQRHIGP
DIDVPAGDIGVGGKEIGYMYGQYRRIRACFENGVLTGKSLESGGSLIRPEATGYGAVFFLDEMLKHDGETLQGKTVVTSG
YGNVAWGVCKKVAQLGGKVVTISGSKGFVHDPEGITTDEKIEFLLKIRNGEKTMQDYAKEFNASWHAGQKPWGIKADIAI
PAATQNEIDFEDAQKLIDSGVKYVVEASNMPTTNEAIEFLMKKGIILAPGKAANAGGVAVSGLEMSQNSARLSWSAEEVE
SKLQQIMANIFHACKFASSKYNLGYNLVAGANLAGFEKVAEAMIQQGRY

Sequences:

>Translated_449_residues
MNAQKYIDSVIAQVEKRDGHEKEFIQAVKEVFSTLKPALEHNPKYIEENILARMVEPERGISFRVPWVDKDGNIQVNRGY
RYQFNGAIGPFKGGIRFHPSVYSGIIKFLGFEQVFKNSLTTLPMGGGKGGADFDPKGKTDAEIMNFCQSFMMELQRHIGP
DIDVPAGDIGVGGKEIGYMYGQYRRIRACFENGVLTGKSLESGGSLIRPEATGYGAVFFLDEMLKHDGETLQGKTVVTSG
YGNVAWGVCKKVAQLGGKVVTISGSKGFVHDPEGITTDEKIEFLLKIRNGEKTMQDYAKEFNASWHAGQKPWGIKADIAI
PAATQNEIDFEDAQKLIDSGVKYVVEASNMPTTNEAIEFLMKKGIILAPGKAANAGGVAVSGLEMSQNSARLSWSAEEVE
SKLQQIMANIFHACKFASSKYNLGYNLVAGANLAGFEKVAEAMIQQGRY
>Mature_449_residues
MNAQKYIDSVIAQVEKRDGHEKEFIQAVKEVFSTLKPALEHNPKYIEENILARMVEPERGISFRVPWVDKDGNIQVNRGY
RYQFNGAIGPFKGGIRFHPSVYSGIIKFLGFEQVFKNSLTTLPMGGGKGGADFDPKGKTDAEIMNFCQSFMMELQRHIGP
DIDVPAGDIGVGGKEIGYMYGQYRRIRACFENGVLTGKSLESGGSLIRPEATGYGAVFFLDEMLKHDGETLQGKTVVTSG
YGNVAWGVCKKVAQLGGKVVTISGSKGFVHDPEGITTDEKIEFLLKIRNGEKTMQDYAKEFNASWHAGQKPWGIKADIAI
PAATQNEIDFEDAQKLIDSGVKYVVEASNMPTTNEAIEFLMKKGIILAPGKAANAGGVAVSGLEMSQNSARLSWSAEEVE
SKLQQIMANIFHACKFASSKYNLGYNLVAGANLAGFEKVAEAMIQQGRY

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=358, Percent_Identity=29.608938547486, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4885281, Length=359, Percent_Identity=30.08356545961, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1788059, Length=451, Percent_Identity=54.9889135254989, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17544676, Length=346, Percent_Identity=30.635838150289, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=47.787610619469, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=47.5770925110132, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24649283, Length=375, Percent_Identity=30.1333333333333, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45549226, Length=358, Percent_Identity=31.0055865921788, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45553475, Length=358, Percent_Identity=31.0055865921788, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24649460, Length=294, Percent_Identity=30.6122448979592, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 49159; Mature: 49159

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQKYIDSVIAQVEKRDGHEKEFIQAVKEVFSTLKPALEHNPKYIEENILARMVEPERG
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
ISFRVPWVDKDGNIQVNRGYRYQFNGAIGPFKGGIRFHPSVYSGIIKFLGFEQVFKNSLT
CEEECCEECCCCCEEEECCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCC
TLPMGGGKGGADFDPKGKTDAEIMNFCQSFMMELQRHIGPDIDVPAGDIGVGGKEIGYMY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GQYRRIRACFENGVLTGKSLESGGSLIRPEATGYGAVFFLDEMLKHDGETLQGKTVVTSG
HHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECC
YGNVAWGVCKKVAQLGGKVVTISGSKGFVHDPEGITTDEKIEFLLKIRNGEKTMQDYAKE
CCCHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCCCHHHHEEHEEECCCHHHHHHHHHH
FNASWHAGQKPWGIKADIAIPAATQNEIDFEDAQKLIDSGVKYVVEASNMPTTNEAIEFL
HCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
MKKGIILAPGKAANAGGVAVSGLEMSQNSARLSWSAEEVESKLQQIMANIFHACKFASSK
HHCCEEECCCCCCCCCCEEEECEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCC
YNLGYNLVAGANLAGFEKVAEAMIQQGRY
CCCCEEEEECCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAQKYIDSVIAQVEKRDGHEKEFIQAVKEVFSTLKPALEHNPKYIEENILARMVEPERG
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
ISFRVPWVDKDGNIQVNRGYRYQFNGAIGPFKGGIRFHPSVYSGIIKFLGFEQVFKNSLT
CEEECCEECCCCCEEEECCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCC
TLPMGGGKGGADFDPKGKTDAEIMNFCQSFMMELQRHIGPDIDVPAGDIGVGGKEIGYMY
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
GQYRRIRACFENGVLTGKSLESGGSLIRPEATGYGAVFFLDEMLKHDGETLQGKTVVTSG
HHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECC
YGNVAWGVCKKVAQLGGKVVTISGSKGFVHDPEGITTDEKIEFLLKIRNGEKTMQDYAKE
CCCHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCCCHHHHEEHEEECCCHHHHHHHHHH
FNASWHAGQKPWGIKADIAIPAATQNEIDFEDAQKLIDSGVKYVVEASNMPTTNEAIEFL
HCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
MKKGIILAPGKAANAGGVAVSGLEMSQNSARLSWSAEEVESKLQQIMANIFHACKFASSK
HHCCEEECCCCCCCCCCEEEECEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCC
YNLGYNLVAGANLAGFEKVAEAMIQQGRY
CCCCEEEEECCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1587267; 1954226; 1633808; 1553382; 8263917; 9405044 [H]