Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 89255707

GI number: 89255707

Start: 261426

End: 261998

Strand: Reverse

Name: ogt [H]

Synonym: FTL_0275

Alternate gene names: 89255707

Gene position: 261998-261426 (Counterclockwise)

Preceding gene: 89255712

Following gene: 89255702

Centisome position: 13.82

GC content: 31.24

Gene sequence:

>573_bases
ATGCTCAAAGAAACTCATCAAATTTATTTAGCTGCGATCAATAAAAAGAGCTATAAAACCGTATTTTATGCTCAAGAGAT
TCAGACGCCTATTGGTAATCTTATTGCAGTTGCTGATGATGATTATCTATATGCTTGCTTTTTTATTAGTGATTCGAATA
TCTATTCTATAGAAAAATTATTAAAAATTTACAATGCAAAAATATCTTTCAATGCAGACAATATAACCAATCAGACTAAA
GATCAACTAAATAAATATTTCAAAAGAGAACTTAAAAGCTTCTCAGTCCCACTACGACTAACAGGCACAGACTTTCAAAA
ACAAGTTTGGCGCGAGCTAATAAAGATCCCTTATGGAGAAACTATCAGCTATCGTAAAGAAGCTACAAATATTGGTAAAC
CAACTGCTTTTAGAGCTGTAGCTAATGCTAATGGTAAGAATCTATTACCCATAATTATTCCTTGTCATCGCGTAATTAAT
ACTAACGGTAAACTTGGTGGCTATACCAGTGGATTATATAAAAAAGAGTTTTTGTTAAATCTAGAACACTCGATTCTAAT
ACACAAAAATTAG

Upstream 100 bases:

>100_bases
ATACCAATAATTAAAAATACTACAGGTAAAAACCTATAAAATTTACTATTAAACATAGTTTGAGTTATCATTTAAAAGAC
ATAATAAACATAATATACCT

Downstream 100 bases:

>100_bases
ATTATAAGTTGCTCAATTATCAATTATCATAAAAATGATAAGCACTTATGCTTTATTATGTCAAACTTTTAATAGGAGAA
AATAATGAAAAAACTTAAAT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKLLKIYNAKISFNADNITNQTK
DQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGETISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVIN
TNGKLGGYTSGLYKKEFLLNLEHSILIHKN

Sequences:

>Translated_190_residues
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKLLKIYNAKISFNADNITNQTK
DQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGETISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVIN
TNGKLGGYTSGLYKKEFLLNLEHSILIHKN
>Mature_190_residues
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKLLKIYNAKISFNADNITNQTK
DQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGETISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVIN
TNGKLGGYTSGLYKKEFLLNLEHSILIHKN

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=163, Percent_Identity=30.6748466257669, Blast_Score=77, Evalue=7e-15,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=36.5269461077844, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1788542, Length=146, Percent_Identity=38.3561643835616, Blast_Score=107, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=50, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=50, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=96, Percent_Identity=45.8333333333333, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137554, Length=92, Percent_Identity=47.8260869565217, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21789; Mature: 21789

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKL
CCCCCEEEEEEEECCCCCEEEEEHHHHCCCCCCEEEEECCCEEEEEEEEECCCCHHHHHH
LKIYNAKISFNADNITNQTKDQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGE
HHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCC
TISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVINTNGKLGGYTSGLYKKEFLLN
CCHHHHHHCCCCCCHHHHHEECCCCCEEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHEE
LEHSILIHKN
CCCEEEEECC
>Mature Secondary Structure
MLKETHQIYLAAINKKSYKTVFYAQEIQTPIGNLIAVADDDYLYACFFISDSNIYSIEKL
CCCCCEEEEEEEECCCCCEEEEEHHHHCCCCCCEEEEECCCEEEEEEEEECCCCHHHHHH
LKIYNAKISFNADNITNQTKDQLNKYFKRELKSFSVPLRLTGTDFQKQVWRELIKIPYGE
HHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCC
TISYRKEATNIGKPTAFRAVANANGKNLLPIIIPCHRVINTNGKLGGYTSGLYKKEFLLN
CCHHHHHHCCCCCCHHHHHEECCCCCEEEEEEEECHHHHCCCCCCCCCCCCHHHHHHHEE
LEHSILIHKN
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA