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The map label for this gene is dfrA [H]

Identifier: 89255657

GI number: 89255657

Start: 224465

End: 225007

Strand: Reverse

Name: dfrA [H]

Synonym: FTL_0223

Alternate gene names: 89255657

Gene position: 225007-224465 (Counterclockwise)

Preceding gene: 89255702

Following gene: 89255656

Centisome position: 11.87

GC content: 27.07

Gene sequence:

>543_bases
ATGCTAAAATACATCAAAATGACTAAAAATAGATTTCTCAAAATGATCTCACTAATCGTTGCTTATGATAAAAACTTTGG
TATAGGTAAAGAAAATACTCTAGCATGGAAACTATCAGAAGATCTTAAGAACTTTAAAAAAATAACAGAAAACAACTATA
TTGTTATGGGTAGAAAAACCTTTGAATCGATTGGTCGCCCACTACCGAATCGTAAAAATATTATTTTAACTAGAAACAAA
GATTACAAACAAGATAAATGTTTAATAATAAATAGTACTCAGGATATTTTAAATTTTGCTGAATCAAAACCACACTACGA
AATATTTATAATCGGTGGTGCACAGATCTATAAAGAATTTCTAAAATACGCAGATAGATTGTATATAACCGAAGTTGATG
CAGAAATGACTGATCTTGATGCTTTCTTTCCACAATGGGATAAATCAAAATATAAACGTATTGGTCACAAACAATTCAAA
AAAAATGATAAAAATGAGTTTGACTTTACATTTAGCATCTTTGAAAAAAATAAAAATTTGTAA

Upstream 100 bases:

>100_bases
TTTATATAATTAAACATTCTACTCATACATGAAGTATAAGCTGTAAAATTTTACCACAGTTAATTTATAAATTACAAAAG
GTATTTTATATTTGTTTTAA

Downstream 100 bases:

>100_bases
TTTAGTCAGACCATTTAAGTAGAATTTTGAGTAATCATAATGTAGATTTAAATTACACAGAATATTAAATTTTTTAATCC
AATGTCAGCTCAAGTCTCAG

Product: dihydrofolate reductase type I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MLKYIKMTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGRPLPNRKNIILTRNK
DYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFK
KNDKNEFDFTFSIFEKNKNL

Sequences:

>Translated_180_residues
MLKYIKMTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGRPLPNRKNIILTRNK
DYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFK
KNDKNEFDFTFSIFEKNKNL
>Mature_180_residues
MLKYIKMTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKTFESIGRPLPNRKNIILTRNK
DYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEFLKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFK
KNDKNEFDFTFSIFEKNKNL

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=186, Percent_Identity=31.1827956989247, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI28827793, Length=187, Percent_Identity=30.4812834224599, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=37.6543209876543, Blast_Score=117, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=30.0546448087432, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=140, Percent_Identity=32.8571428571429, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116008026, Length=178, Percent_Identity=31.4606741573034, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647458, Length=178, Percent_Identity=31.4606741573034, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 21497; Mature: 21497

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKYIKMTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECHH
FESIGRPLPNRKNIILTRNKDYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEF
HHHHCCCCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEECHHHHHHH
LKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKNDKNEFDFTFSIFEKNKNL
HHHHHEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
>Mature Secondary Structure
MLKYIKMTKNRFLKMISLIVAYDKNFGIGKENTLAWKLSEDLKNFKKITENNYIVMGRKT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECHH
FESIGRPLPNRKNIILTRNKDYKQDKCLIINSTQDILNFAESKPHYEIFIIGGAQIYKEF
HHHHCCCCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEECHHHHHHH
LKYADRLYITEVDAEMTDLDAFFPQWDKSKYKRIGHKQFKKNDKNEFDFTFSIFEKNKNL
HHHHHEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]