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The map label for this gene is gsiB [H]

Identifier: 89056625

GI number: 89056625

Start: 4243673

End: 4245223

Strand: Direct

Name: gsiB [H]

Synonym: Jann_4134

Alternate gene names: 89056625

Gene position: 4243673-4245223 (Clockwise)

Preceding gene: 89056624

Following gene: 89056631

Centisome position: 98.28

GC content: 61.06

Gene sequence:

>1551_bases
ATGATAAAATCTACCCATTTGCTTGCAGCCGGTGCTGCCAGCTTGGCCCTCACGACGGGCGCGTTTGCCCAGGCGGACGA
TACGCTTGTTGTCGGCCTCAGCACAGATATCAGCACGCTGGACCCCGCGCCGATCTCTTCGCGCGACAATTCCAACATCG
CGCGCCACATCTTCGGCACGCTCTATACGATGAGCGGCGATGGTGAGGCCCTGCCGGTGCTGGCCAACAACCTTGAGATC
TCCGACGATGGGCTGGCCTACGTCTATACACTGAATGAGGGTCTGACCTGCCACGATGGCGAAGCGCTGACAGCGGAAGA
TGCGGCCTATTCCTTCAACCGCGCTGCTGATCCGGAAAATGCCTTCACCGGCAACACGCCCGGCTTCATCTTTTCCTCGA
TCGATTTTCAAGGCGCAGAAGCGCTGGACGAGCTGCGCGTGCAGGTGAATATCGGCGCGCCAAACCCGATTGCCTTCGGC
CTGATCGCGGAGGTGTTCATCCACTGCATGGACAGCTATGAGGCGATGACCCTTGATGAGGCGGCCAGCAATCCGATTGG
ATCCGGCCACTACCGCCTGGTGGAATGGCGTCGCGGTTCCGAGGTCGTGCTTGAGGCTGTCGAAGGCGCAGAGGTGGGCT
TCCAGAACCTCGTCTGGCGGATCATCCCCGAAGCCTCCACTCGCGCGGCGGAACTGATGGCCGGTTCCGTTGACATCATC
ACCAATGTGGCCCCGGACCAGATGGATGTGATCAACGCATCGGGCGCGGCAGAGGTGAACCCGATCCAGGGCACACGCCG
CATGTATGTGGGCTTCAACCTGTCCGAGACGATGGCGCAGGAACCCGGCGGCGACGCGATCCAGGATCCGGCCGTGCGTC
GGGCGCTGCAATATGCGGTCAACGTTCCGGCGATTTGTTCGCAGCTTCTGAACTTTGAGTGTGAGCGGATGACCGGCATC
GTGAACCCGCCCAATGCCAACCAGAGCCTGGAGCCCTACCCCTATGATCCGGAAGAGGCCGAGCGTCTGCTGGATGAGGC
GGGTTGGCCCCGGGGCGACGATGGCACCCGCTTCTCAATCGCCTTCCAAGCCGGTCAGGGTCGTTACCTGAATGACGCCA
ACGTCGTGCAGGCCATCGCCCAATATCTCAGCGATGTGGGTCTGGATGTCGACTTGCAGATCATGGAATGGTCCAGCGTC
TACATTCCGATCATCCGGGAACGCAATGCTGGCCCGCTCTACTTCATCGGCTCCGGTGGGGCGTTGTGGAGCCCGCTCTA
TGACATGACCGACCTGGCCGCTGTCGATTCAGGCACCAACTACACCCATTGGGATGACCCCCGCTGGTTTGACCGTTGGT
CCGACATCGCCGCCGCCGAGACGGAGGAGGAGACCCGCGAGATCGTGGATGAGATGTTGCAGGTCTTCTACGATGATGGC
CCCTGGCTGCACCTCTACTTCCAGCCGGACTTCTACGGTGTGTCCAACCGCGTGAACTGGACCCCGCGGCCCGATGAGAA
GGTTTACCTCTGGGATGCGACGCTGAACTAA

Upstream 100 bases:

>100_bases
TGGGCCTTCGGTGCAGACCGCAACCCGGACTGGAACCTGACATGCAAAAGGTCAGGGCACGGTTCACCGAAGACCACGCA
ACAGGTGAAAGGAAAGCCAA

Downstream 100 bases:

>100_bases
GCCGCATTCTGATACGATTAGGGGGCGTCCGATCTACCGCCGGGCGCCCCTGTTTTTTGTCCGCGAGGGCCTGTTGGCGT
GCGAAATCGTCAGAACCGAG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MIKSTHLLAAGAASLALTTGAFAQADDTLVVGLSTDISTLDPAPISSRDNSNIARHIFGTLYTMSGDGEALPVLANNLEI
SDDGLAYVYTLNEGLTCHDGEALTAEDAAYSFNRAADPENAFTGNTPGFIFSSIDFQGAEALDELRVQVNIGAPNPIAFG
LIAEVFIHCMDSYEAMTLDEAASNPIGSGHYRLVEWRRGSEVVLEAVEGAEVGFQNLVWRIIPEASTRAAELMAGSVDII
TNVAPDQMDVINASGAAEVNPIQGTRRMYVGFNLSETMAQEPGGDAIQDPAVRRALQYAVNVPAICSQLLNFECERMTGI
VNPPNANQSLEPYPYDPEEAERLLDEAGWPRGDDGTRFSIAFQAGQGRYLNDANVVQAIAQYLSDVGLDVDLQIMEWSSV
YIPIIRERNAGPLYFIGSGGALWSPLYDMTDLAAVDSGTNYTHWDDPRWFDRWSDIAAAETEEETREIVDEMLQVFYDDG
PWLHLYFQPDFYGVSNRVNWTPRPDEKVYLWDATLN

Sequences:

>Translated_516_residues
MIKSTHLLAAGAASLALTTGAFAQADDTLVVGLSTDISTLDPAPISSRDNSNIARHIFGTLYTMSGDGEALPVLANNLEI
SDDGLAYVYTLNEGLTCHDGEALTAEDAAYSFNRAADPENAFTGNTPGFIFSSIDFQGAEALDELRVQVNIGAPNPIAFG
LIAEVFIHCMDSYEAMTLDEAASNPIGSGHYRLVEWRRGSEVVLEAVEGAEVGFQNLVWRIIPEASTRAAELMAGSVDII
TNVAPDQMDVINASGAAEVNPIQGTRRMYVGFNLSETMAQEPGGDAIQDPAVRRALQYAVNVPAICSQLLNFECERMTGI
VNPPNANQSLEPYPYDPEEAERLLDEAGWPRGDDGTRFSIAFQAGQGRYLNDANVVQAIAQYLSDVGLDVDLQIMEWSSV
YIPIIRERNAGPLYFIGSGGALWSPLYDMTDLAAVDSGTNYTHWDDPRWFDRWSDIAAAETEEETREIVDEMLQVFYDDG
PWLHLYFQPDFYGVSNRVNWTPRPDEKVYLWDATLN
>Mature_516_residues
MIKSTHLLAAGAASLALTTGAFAQADDTLVVGLSTDISTLDPAPISSRDNSNIARHIFGTLYTMSGDGEALPVLANNLEI
SDDGLAYVYTLNEGLTCHDGEALTAEDAAYSFNRAADPENAFTGNTPGFIFSSIDFQGAEALDELRVQVNIGAPNPIAFG
LIAEVFIHCMDSYEAMTLDEAASNPIGSGHYRLVEWRRGSEVVLEAVEGAEVGFQNLVWRIIPEASTRAAELMAGSVDII
TNVAPDQMDVINASGAAEVNPIQGTRRMYVGFNLSETMAQEPGGDAIQDPAVRRALQYAVNVPAICSQLLNFECERMTGI
VNPPNANQSLEPYPYDPEEAERLLDEAGWPRGDDGTRFSIAFQAGQGRYLNDANVVQAIAQYLSDVGLDVDLQIMEWSSV
YIPIIRERNAGPLYFIGSGGALWSPLYDMTDLAAVDSGTNYTHWDDPRWFDRWSDIAAAETEEETREIVDEMLQVFYDDG
PWLHLYFQPDFYGVSNRVNWTPRPDEKVYLWDATLN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=409, Percent_Identity=27.1393643031785, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789966, Length=533, Percent_Identity=23.6397748592871, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789397, Length=485, Percent_Identity=23.2989690721649, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789887, Length=498, Percent_Identity=22.4899598393574, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787762, Length=313, Percent_Identity=24.6006389776358, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787495, Length=435, Percent_Identity=22.0689655172414, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081878, Length=478, Percent_Identity=22.8033472803347, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56515; Mature: 56515

Theoretical pI: Translated: 3.89; Mature: 3.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSTHLLAAGAASLALTTGAFAQADDTLVVGLSTDISTLDPAPISSRDNSNIARHIFGT
CCCCCEEEECCCHHHEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHEEE
LYTMSGDGEALPVLANNLEISDDGLAYVYTLNEGLTCHDGEALTAEDAAYSFNRAADPEN
EEEECCCCCEEEEEECCEEECCCCEEEEEEECCCCEECCCCEEECCHHHHCCCCCCCCCC
AFTGNTPGFIFSSIDFQGAEALDELRVQVNIGAPNPIAFGLIAEVFIHCMDSYEAMTLDE
CCCCCCCCEEEEECCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHH
AASNPIGSGHYRLVEWRRGSEVVLEAVEGAEVGFQNLVWRIIPEASTRAAELMAGSVDII
HHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHEEECCCCCHHHHHHHCCCCCEE
TNVAPDQMDVINASGAAEVNPIQGTRRMYVGFNLSETMAQEPGGDAIQDPAVRRALQYAV
ECCCCCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHC
NVPAICSQLLNFECERMTGIVNPPNANQSLEPYPYDPEEAERLLDEAGWPRGDDGTRFSI
CCHHHHHHHHCCEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEE
AFQAGQGRYLNDANVVQAIAQYLSDVGLDVDLQIMEWSSVYIPIIRERNAGPLYFIGSGG
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCEEEEECCC
ALWSPLYDMTDLAAVDSGTNYTHWDDPRWFDRWSDIAAAETEEETREIVDEMLQVFYDDG
CHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
PWLHLYFQPDFYGVSNRVNWTPRPDEKVYLWDATLN
CEEEEEECCCEECCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MIKSTHLLAAGAASLALTTGAFAQADDTLVVGLSTDISTLDPAPISSRDNSNIARHIFGT
CCCCCEEEECCCHHHEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHEEE
LYTMSGDGEALPVLANNLEISDDGLAYVYTLNEGLTCHDGEALTAEDAAYSFNRAADPEN
EEEECCCCCEEEEEECCEEECCCCEEEEEEECCCCEECCCCEEECCHHHHCCCCCCCCCC
AFTGNTPGFIFSSIDFQGAEALDELRVQVNIGAPNPIAFGLIAEVFIHCMDSYEAMTLDE
CCCCCCCCEEEEECCCCCHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHH
AASNPIGSGHYRLVEWRRGSEVVLEAVEGAEVGFQNLVWRIIPEASTRAAELMAGSVDII
HHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHEEECCCCCHHHHHHHCCCCCEE
TNVAPDQMDVINASGAAEVNPIQGTRRMYVGFNLSETMAQEPGGDAIQDPAVRRALQYAV
ECCCCCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHC
NVPAICSQLLNFECERMTGIVNPPNANQSLEPYPYDPEEAERLLDEAGWPRGDDGTRFSI
CCHHHHHHHHCCEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEE
AFQAGQGRYLNDANVVQAIAQYLSDVGLDVDLQIMEWSSVYIPIIRERNAGPLYFIGSGG
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCEEEEECCC
ALWSPLYDMTDLAAVDSGTNYTHWDDPRWFDRWSDIAAAETEEETREIVDEMLQVFYDDG
CHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
PWLHLYFQPDFYGVSNRVNWTPRPDEKVYLWDATLN
CEEEEEECCCEECCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA