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The map label for this gene is ilvE [H]

Identifier: 89055343

GI number: 89055343

Start: 2872494

End: 2873420

Strand: Direct

Name: ilvE [H]

Synonym: Jann_2852

Alternate gene names: 89055343

Gene position: 2872494-2873420 (Clockwise)

Preceding gene: 89055342

Following gene: 89055345

Centisome position: 66.52

GC content: 60.84

Gene sequence:

>927_bases
ATGTCAGACACGCACACCACCCACGACGCCCTGGAAGATCCGCGCAACGCGGACATCCTGATCTATGTGAACGGCGATCT
CGTTCACCGGGACGACGCGAAGGTCTCGGTCTATGACAGCGGGTTTCTGCTTGGCGATGGCATGTGGGAAGGTCTGCGCC
TTTATGACGGGGTGTGGAGCTTTTTTGACGACCACATGGACCGCTTCTTCGACAGTTGCAAAGCGGTGTCGCTGGATGTG
GGCATGGACCGAGCCGGAGTTTTCGCGGCGCTGGAGGCCACGCGGAAGGCCAACGATATGCACACGGATGTGCATTGCCG
CCTGATGCTGACCCGTGGCGTGAAAGTGAAGCCGTTCCAGCACCCATCGCTATCGCGCTCCGGCCCGACGCTGGTGATTA
TCTGTGAGCATTCCAAGCCGGTTGACCGGCTGCAGGGCGCGGGCATCCGGCTGGCGACCGTCCCGCAGGTGCGCGGATTG
CCGATGAGCCAGGACGCGAAATACAACAGCCATTCCAAGCTGAATTGCGTCATTGCCTGCCTGCAGGCCGAACAGGCGGG
CGCAGATGAGGGGTTGATGCTGGACCCCAATGGCTTCGTGAACACCACCAATGCGTGCAACTTCTTCATCGTGCGCAAGG
GGGAGGTTTGGACCTCCACCGGGGATTATTGCATGAACGGGGTGACGCGGCAGAAGGTGATCGACCTGTGCCGGGCCGAG
GGGATTGCGGTGTTCGAGAAGAACTTCTCGCTCTACGAGGCTGTGTCGGCAGATGAGGCGTTTCTGACCGGTACCTTCGG
GGCACAGACCCCGGTGGCGGAGATCGACGGGAAGGCCATTGGTAATGGCGAACGCCCCATCACCACACGCATTCAGGCCG
CCTATAAGGCGTTGATCGCGCACCATATTGCGGAGATGCGCAGCTAG

Upstream 100 bases:

>100_bases
CGTGGTGCCTGAGCGCGCGCCGAAATTCCAGAGGGCACGGACAACCGTGACAAGTCGGCCCGCCTTCGCCACAAAGGGCG
CAACAGCAGCGAGGCCGCAG

Downstream 100 bases:

>100_bases
GCCGCACGCCGCCCCGCCGACCAGACCGATTGCAGCACCGGCACGCCGTCCATCGTGCGAAAGCGAATGACATCCGCCCG
TTGCCCCACGGCCAGAGCCC

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSDTHTTHDALEDPRNADILIYVNGDLVHRDDAKVSVYDSGFLLGDGMWEGLRLYDGVWSFFDDHMDRFFDSCKAVSLDV
GMDRAGVFAALEATRKANDMHTDVHCRLMLTRGVKVKPFQHPSLSRSGPTLVIICEHSKPVDRLQGAGIRLATVPQVRGL
PMSQDAKYNSHSKLNCVIACLQAEQAGADEGLMLDPNGFVNTTNACNFFIVRKGEVWTSTGDYCMNGVTRQKVIDLCRAE
GIAVFEKNFSLYEAVSADEAFLTGTFGAQTPVAEIDGKAIGNGERPITTRIQAAYKALIAHHIAEMRS

Sequences:

>Translated_308_residues
MSDTHTTHDALEDPRNADILIYVNGDLVHRDDAKVSVYDSGFLLGDGMWEGLRLYDGVWSFFDDHMDRFFDSCKAVSLDV
GMDRAGVFAALEATRKANDMHTDVHCRLMLTRGVKVKPFQHPSLSRSGPTLVIICEHSKPVDRLQGAGIRLATVPQVRGL
PMSQDAKYNSHSKLNCVIACLQAEQAGADEGLMLDPNGFVNTTNACNFFIVRKGEVWTSTGDYCMNGVTRQKVIDLCRAE
GIAVFEKNFSLYEAVSADEAFLTGTFGAQTPVAEIDGKAIGNGERPITTRIQAAYKALIAHHIAEMRS
>Mature_307_residues
SDTHTTHDALEDPRNADILIYVNGDLVHRDDAKVSVYDSGFLLGDGMWEGLRLYDGVWSFFDDHMDRFFDSCKAVSLDVG
MDRAGVFAALEATRKANDMHTDVHCRLMLTRGVKVKPFQHPSLSRSGPTLVIICEHSKPVDRLQGAGIRLATVPQVRGLP
MSQDAKYNSHSKLNCVIACLQAEQAGADEGLMLDPNGFVNTTNACNFFIVRKGEVWTSTGDYCMNGVTRQKVIDLCRAEG
IAVFEKNFSLYEAVSADEAFLTGTFGAQTPVAEIDGKAIGNGERPITTRIQAAYKALIAHHIAEMRS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=291, Percent_Identity=29.8969072164948, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787338, Length=258, Percent_Identity=28.6821705426357, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 33848; Mature: 33717

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTHTTHDALEDPRNADILIYVNGDLVHRDDAKVSVYDSGFLLGDGMWEGLRLYDGVWS
CCCCCCCHHHHCCCCCCEEEEEECCCEEECCCCEEEEEECCEEEECCCHHHHHHHHHHHH
FFDDHMDRFFDSCKAVSLDVGMDRAGVFAALEATRKANDMHTDVHCRLMLTRGVKVKPFQ
HHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCEECCCC
HPSLSRSGPTLVIICEHSKPVDRLQGAGIRLATVPQVRGLPMSQDAKYNSHSKLNCVIAC
CCCCCCCCCEEEEEECCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEE
LQAEQAGADEGLMLDPNGFVNTTNACNFFIVRKGEVWTSTGDYCMNGVTRQKVIDLCRAE
EHHHHCCCCCCEEECCCCCEECCCCEEEEEEECCCEEECCCHHHHCCCCHHHHHHHHHCC
GIAVFEKNFSLYEAVSADEAFLTGTFGAQTPVAEIDGKAIGNGERPITTRIQAAYKALIA
CEEEEECCCCHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
HHIAEMRS
HHHHHHCC
>Mature Secondary Structure 
SDTHTTHDALEDPRNADILIYVNGDLVHRDDAKVSVYDSGFLLGDGMWEGLRLYDGVWS
CCCCCCHHHHCCCCCCEEEEEECCCEEECCCCEEEEEECCEEEECCCHHHHHHHHHHHH
FFDDHMDRFFDSCKAVSLDVGMDRAGVFAALEATRKANDMHTDVHCRLMLTRGVKVKPFQ
HHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCEECCCC
HPSLSRSGPTLVIICEHSKPVDRLQGAGIRLATVPQVRGLPMSQDAKYNSHSKLNCVIAC
CCCCCCCCCEEEEEECCCCCHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEE
LQAEQAGADEGLMLDPNGFVNTTNACNFFIVRKGEVWTSTGDYCMNGVTRQKVIDLCRAE
EHHHHCCCCCCEEECCCCCEECCCCEEEEEEECCCEEECCCHHHHCCCCHHHHHHHHHCC
GIAVFEKNFSLYEAVSADEAFLTGTFGAQTPVAEIDGKAIGNGERPITTRIQAAYKALIA
CEEEEECCCCHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
HHIAEMRS
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]