Click here to switch to the map view.

The map label for this gene is ltaA [H]

Identifier: 89055342

GI number: 89055342

Start: 2871077

End: 2872108

Strand: Direct

Name: ltaA [H]

Synonym: Jann_2851

Alternate gene names: 89055342

Gene position: 2871077-2872108 (Clockwise)

Preceding gene: 89055340

Following gene: 89055343

Centisome position: 66.49

GC content: 67.25

Gene sequence:

>1032_bases
ATGTCCCAATATACCGCCGCCGCCATGCCCGCCGGCCCGATTGCCGCCGACCTTCGGTCTGACACGGTTACGCAGCCCTG
TGCGGCCATGCGCGCGGCGATGGCAGGGGCCGAGGTGGGCGATGACGTTTACGGCGACGACCCCTGCATCAACCGGCTGG
AGGCCTCTCTGGCCGAGCGGTTGGGCAAGGACGAGGGGCTGTTTGTCCCGTCCGGCACGCAGTCGAACCTTCTCGCTATG
CTTGCCCATTGCGGCCGGGGTGAAGAGGTGATCGCGGGCGACAGCTACCACGTGATCCGCTACGAGGCGGCGGGGGCGTC
GGTCTTGGGGGGCGTGCCGCTTCACGCGCTGAACGTGGCGGACGATGGTGCGGTCGAGGCGGGGGATGTGACGGCGGCGA
TCAAGGACGACGACAGCCACCACCCGATCAGCCGTTTGTTATCTTTGGAGAATACCAATTACGGACGCGCCATCCCCCTG
CCCCGCATGACAGCCGCGATTGACGCGGGCCGCGCGGGTGGCCTCAACCTGCATCTGGACGGCGCGCGGTTTTTCAACGC
GATCACGGCTCTTGGGTGCACGGAGACGGAGCTTGCGGGTCCGTTCGACACGGTGTCCGTCTGCCTCTCCAAAGGGTTGG
GGGCCCCGGTGGGCTCGGTCCTCGTCGGGCCTCCGGACCTGCTGGCCGCCGCGCGGCGGATGCGAAAGATGCTTGGCGGC
GGCATGCGGCAGGCGGGTGTTCTGGCCGCCGCCGGGCTTCACGCCCTAGAACACAATGTTAAAAGATTGGCCGACGATCA
CGCCCGGGCCGAAGCGCTGGCCGCACATCTGTCCAATCTGGGGCTCGGCGACGTCAGCCAGGCCACCAACATGGTTTTCC
TGACCCCGCATCCGGGCGTGGATCTGGCCCCCCTTGCGGACCACGGTATCCGAACCTCTGCGCCGGAACCCGCCCTGCGG
CTGGTCCTGCACCGTGACATCGACGATGGGGCCTTCGACACGGTTCTGAAGGCATTCTCAGCCTGCGCCTAA

Upstream 100 bases:

>100_bases
ATGGGGAATGCCGCCGAAAACAACAAGGCCAAGGGCGCGGTAGGGGTTGCGGACGGTCGGCTGCCATGGTGCTTTGCCGG
GACGATACAGGAGCCGCCCG

Downstream 100 bases:

>100_bases
GGGGTGGCGACGCTTGGGGCCGGACCGCCCCGCTCCATCATCAGGCTGGCAAGGTAGGGCACCGATAGGCCGAGGGCGAT
GATCGCCCCCACGATGATCA

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSQYTAAAMPAGPIAADLRSDTVTQPCAAMRAAMAGAEVGDDVYGDDPCINRLEASLAERLGKDEGLFVPSGTQSNLLAM
LAHCGRGEEVIAGDSYHVIRYEAAGASVLGGVPLHALNVADDGAVEAGDVTAAIKDDDSHHPISRLLSLENTNYGRAIPL
PRMTAAIDAGRAGGLNLHLDGARFFNAITALGCTETELAGPFDTVSVCLSKGLGAPVGSVLVGPPDLLAAARRMRKMLGG
GMRQAGVLAAAGLHALEHNVKRLADDHARAEALAAHLSNLGLGDVSQATNMVFLTPHPGVDLAPLADHGIRTSAPEPALR
LVLHRDIDDGAFDTVLKAFSACA

Sequences:

>Translated_343_residues
MSQYTAAAMPAGPIAADLRSDTVTQPCAAMRAAMAGAEVGDDVYGDDPCINRLEASLAERLGKDEGLFVPSGTQSNLLAM
LAHCGRGEEVIAGDSYHVIRYEAAGASVLGGVPLHALNVADDGAVEAGDVTAAIKDDDSHHPISRLLSLENTNYGRAIPL
PRMTAAIDAGRAGGLNLHLDGARFFNAITALGCTETELAGPFDTVSVCLSKGLGAPVGSVLVGPPDLLAAARRMRKMLGG
GMRQAGVLAAAGLHALEHNVKRLADDHARAEALAAHLSNLGLGDVSQATNMVFLTPHPGVDLAPLADHGIRTSAPEPALR
LVLHRDIDDGAFDTVLKAFSACA
>Mature_342_residues
SQYTAAAMPAGPIAADLRSDTVTQPCAAMRAAMAGAEVGDDVYGDDPCINRLEASLAERLGKDEGLFVPSGTQSNLLAML
AHCGRGEEVIAGDSYHVIRYEAAGASVLGGVPLHALNVADDGAVEAGDVTAAIKDDDSHHPISRLLSLENTNYGRAIPLP
RMTAAIDAGRAGGLNLHLDGARFFNAITALGCTETELAGPFDTVSVCLSKGLGAPVGSVLVGPPDLLAAARRMRKMLGGG
MRQAGVLAAAGLHALEHNVKRLADDHARAEALAAHLSNLGLGDVSQATNMVFLTPHPGVDLAPLADHGIRTSAPEPALRL
VLHRDIDDGAFDTVLKAFSACA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=315, Percent_Identity=46.984126984127, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI193206522, Length=342, Percent_Identity=42.1052631578947, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI193206520, Length=342, Percent_Identity=42.1052631578947, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI193206524, Length=340, Percent_Identity=42.3529411764706, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320789, Length=344, Percent_Identity=35.1744186046512, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21355439, Length=363, Percent_Identity=43.5261707988981, Blast_Score=243, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 35324; Mature: 35193

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYTAAAMPAGPIAADLRSDTVTQPCAAMRAAMAGAEVGDDVYGDDPCINRLEASLAER
CCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LGKDEGLFVPSGTQSNLLAMLAHCGRGEEVIAGDSYHVIRYEAAGASVLGGVPLHALNVA
CCCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCHHCCCCCEEEEECC
DDGAVEAGDVTAAIKDDDSHHPISRLLSLENTNYGRAIPLPRMTAAIDAGRAGGLNLHLD
CCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEC
GARFFNAITALGCTETELAGPFDTVSVCLSKGLGAPVGSVLVGPPDLLAAARRMRKMLGG
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCC
GMRQAGVLAAAGLHALEHNVKRLADDHARAEALAAHLSNLGLGDVSQATNMVFLTPHPGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEECCCCCC
DLAPLADHGIRTSAPEPALRLVLHRDIDDGAFDTVLKAFSACA
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQYTAAAMPAGPIAADLRSDTVTQPCAAMRAAMAGAEVGDDVYGDDPCINRLEASLAER
CCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LGKDEGLFVPSGTQSNLLAMLAHCGRGEEVIAGDSYHVIRYEAAGASVLGGVPLHALNVA
CCCCCCEEECCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCHHCCCCCEEEEECC
DDGAVEAGDVTAAIKDDDSHHPISRLLSLENTNYGRAIPLPRMTAAIDAGRAGGLNLHLD
CCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEC
GARFFNAITALGCTETELAGPFDTVSVCLSKGLGAPVGSVLVGPPDLLAAARRMRKMLGG
HHHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCC
GMRQAGVLAAAGLHALEHNVKRLADDHARAEALAAHLSNLGLGDVSQATNMVFLTPHPGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEECCCCCC
DLAPLADHGIRTSAPEPALRLVLHRDIDDGAFDTVLKAFSACA
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.12 {DL-threo-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]