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The map label for this gene is kipI [H]

Identifier: 89055294

GI number: 89055294

Start: 2815900

End: 2816565

Strand: Direct

Name: kipI [H]

Synonym: Jann_2803

Alternate gene names: 89055294

Gene position: 2815900-2816565 (Clockwise)

Preceding gene: 89055293

Following gene: 89055295

Centisome position: 65.21

GC content: 63.21

Gene sequence:

>666_bases
ATGTTGTCCTTCAAGCCCGCGGGCGATACAGGCCTGCTTGTGACCGTCGCCGAAACGGCATCAGACGCGGCCAATGACCG
GGTGATCGCCCTGGATCACGCGGTGACAGCGGCGCAGATAGACGGCGTCCTTGAAACCATCCCTGCGCTTGTGAACCTCA
TGGTGCACTTCGACCCGTTGGTCACGGATCACGCAGCGCTTGAGGCCGCCACCCGCGCCCTGTTCCCGCTGCCCGAAATG
GCCGCGCAGGATACCCGGACCCACGTGATCCCGGTTTGCTACGACGCAGAACTTTGCCCTGACCTGTTGGCAACGGCCAA
GGCCACCGGGCTGTCGATCGACGCCGTTATCGATGCCCATTCGTCCGCGCGACTGCGCGTGTCCATGTACGGATTTGCAC
CCGGTTACGCCTATCTGTCGGGCCTGCCGCCGAACCTCCATGTGCCGCGCAAAACAACGGCCCTGCGCGATGTGCCCAAG
GGCTCGGTTCTGATCGCAGGGGGGCAGAGCATTTTGACGCCACTCACCATGCCCACGGGCTGGAACATCATCGGCCGCTC
TCCCGCAGAGGTCATTCAGGACGACAAATTCCTGTTCAACGTCGGCGACACGGTCACCTTCACACGCGTCGCCCGCCCCG
ATTTGCCCGAGCGATTGCAGGTATGA

Upstream 100 bases:

>100_bases
ATGGCGATAGCCCCGGCGCGGTCACAATGGCGTCAGCGATCCGCATGAAGCTCGTCTCGCAAGGCATTATGATCAAATCG
TTTCTCTCCGCGTGACCGCA

Downstream 100 bases:

>100_bases
GCGTGGCCCGGTTCAAGGTGCTGTTCGCTGGTCCGTTGGTGTCGTTCCAGGATGGCGGCCGCTTCGGCCACATGCGCTTT
GGCGTGCCCGCGTCCGGCCC

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLSFKPAGDTGLLVTVAETASDAANDRVIALDHAVTAAQIDGVLETIPALVNLMVHFDPLVTDHAALEAATRALFPLPEM
AAQDTRTHVIPVCYDAELCPDLLATAKATGLSIDAVIDAHSSARLRVSMYGFAPGYAYLSGLPPNLHVPRKTTALRDVPK
GSVLIAGGQSILTPLTMPTGWNIIGRSPAEVIQDDKFLFNVGDTVTFTRVARPDLPERLQV

Sequences:

>Translated_221_residues
MLSFKPAGDTGLLVTVAETASDAANDRVIALDHAVTAAQIDGVLETIPALVNLMVHFDPLVTDHAALEAATRALFPLPEM
AAQDTRTHVIPVCYDAELCPDLLATAKATGLSIDAVIDAHSSARLRVSMYGFAPGYAYLSGLPPNLHVPRKTTALRDVPK
GSVLIAGGQSILTPLTMPTGWNIIGRSPAEVIQDDKFLFNVGDTVTFTRVARPDLPERLQV
>Mature_221_residues
MLSFKPAGDTGLLVTVAETASDAANDRVIALDHAVTAAQIDGVLETIPALVNLMVHFDPLVTDHAALEAATRALFPLPEM
AAQDTRTHVIPVCYDAELCPDLLATAKATGLSIDAVIDAHSSARLRVSMYGFAPGYAYLSGLPPNLHVPRKTTALRDVPK
GSVLIAGGQSILTPLTMPTGWNIIGRSPAEVIQDDKFLFNVGDTVTFTRVARPDLPERLQV

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=205, Percent_Identity=32.6829268292683, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 23489; Mature: 23489

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFKPAGDTGLLVTVAETASDAANDRVIALDHAVTAAQIDGVLETIPALVNLMVHFDPL
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCH
VTDHAALEAATRALFPLPEMAAQDTRTHVIPVCYDAELCPDLLATAKATGLSIDAVIDAH
HHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEEEEECC
SSARLRVSMYGFAPGYAYLSGLPPNLHVPRKTTALRDVPKGSVLIAGGQSILTPLTMPTG
CCCEEEEEEEECCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHCCEEECCCC
WNIIGRSPAEVIQDDKFLFNVGDTVTFTRVARPDLPERLQV
CCEECCCHHHHHCCCCEEEECCCEEEEEEECCCCCHHHCCC
>Mature Secondary Structure
MLSFKPAGDTGLLVTVAETASDAANDRVIALDHAVTAAQIDGVLETIPALVNLMVHFDPL
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCH
VTDHAALEAATRALFPLPEMAAQDTRTHVIPVCYDAELCPDLLATAKATGLSIDAVIDAH
HHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHCCCEEEEEEECC
SSARLRVSMYGFAPGYAYLSGLPPNLHVPRKTTALRDVPKGSVLIAGGQSILTPLTMPTG
CCCEEEEEEEECCCCHHHHCCCCCCCCCCCCHHHHHCCCCCCEEEECCCHHCCEEECCCC
WNIIGRSPAEVIQDDKFLFNVGDTVTFTRVARPDLPERLQV
CCEECCCHHHHHCCCCEEEECCCEEEEEEECCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]