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The map label for this gene is murF [H]

Identifier: 89055256

GI number: 89055256

Start: 2779944

End: 2781365

Strand: Reverse

Name: murF [H]

Synonym: Jann_2765

Alternate gene names: 89055256

Gene position: 2781365-2779944 (Counterclockwise)

Preceding gene: 89055257

Following gene: 89055255

Centisome position: 64.41

GC content: 64.63

Gene sequence:

>1422_bases
ATGATTTTGTGGACTTCGGCAGACGCGGCATCGGCCACGGGCGGCACAAGTCAGGGCACCTGGACCGCGACGGGTGTGTC
CATCGACACCCGCACGATTGAGCCGGGCGATCTGTTCGTGGCCTTGGCCGCTGCCCGCGACGGCCATGAGTTCGTTGCCC
AAGCCTTTCAGAAGGGCGCGAGCGCCGCACTTGTTAACCATGTTCCCGACGGTATGGACGCCGCGAAGTGCCTCGTTGTG
GACGATGTGCTTGCCGCGTTGGAGCGGCTTGGAACGGCGGCCCGCACCCGCACGCAGGCCAAGGTCATCGCGATCACGGG
CTCTGTCGGCAAAACCACCGTGAAGGACATGCTGAAGCAGGCGCTGAGCGGGCAGGGTAAGATCCACGCCGCCGTCGCCT
CCTACAACAACCATTGGGGCGTGCCGTTGACGCTGTCGCGTATGCCCGTTGACACAGATTATGCCGTCATCGAGATCGGC
ATGAGTGCCCCCGGCGAAATCGCACCACTGTCTCGCATGGCCCGGCCCCACGTGGCGATGATCACGACAATCGCGCCCGC
GCATCTGGAGGCTTTCGGCCGCATCGAAGGAATTGCCGAGGAAAAGGCGAGTATTTTCGAGGGTTTGGAACCGGACGGCG
TGGCGATTGTGCCAATGGACGTAGAGACGGCTCCGATCCTCGCCCGAGCCGCGGTGGCACATGCAGCAGAGGTTGTGACC
TTTGGCGAGGCCGAGGGCGCGGACCTGCGCCTAACCGATGTGATGCTGAGCGACGACAGCACCGTCATGCGGATCGCCAC
CGACGAAGGGGGGCGTTTGGCGAAGCTCTCGGTCTCTGGTCGCCACTACGCCGTCAATGCCGTGGGCGTTCTTTGCGCCG
TTGCGGCCTCGGGCGCGGATACGACGCAGGCGGCGATGGCACTGTCCCATTGGCAACCGGTGGAGGGACGCGGCCAGCGG
GAGCGCATCCAACTGAGCCGGACCGACGACATCGCCATCGAACTGATCGACGACGCTTTCAACGCCAACCCGACCTCTCT
CGCAGCCGGTCTGGAGGTCCTTGCGGCGATCACGCCGGAGCGCGGGGGGCGGCGCATCGCCGTTTTGGGCGACATGCTGG
AACTGGGGGATGATGGCGCGAAAATGCACGCAGATATTGCCTCACTTCCCGCGATGGAGGCGGTGGATATCGTCCATACC
TGCGGTCCGCTGATGGTGCATCTCGACGCGGCTTTGCCCGGTGCAAAGCGGGGGGAACACACTGAAACGGCGCAAGATCT
GTGCCGGGCAGTGCCGCGCACCCTGCGTCCCGGTGACGTTGTGCTGGTCAAGGGGTCCAAGGGCTCTCGCGTGTCGCTGG
TGGTTGACGCCCTTCGGAAACTGGGGCAATCGCTTGAGCGCGCAGGGCAAGGAAACCGCTAG

Upstream 100 bases:

>100_bases
GAAAGGGCCATGAGACGGGCCAGCAAGTGGGCGATGTCATCTACCCGTTTGACGATGTCGAACAGGCGTCGGTCGCGGTT
GCCGCCCTGGAGGGCGCGAT

Downstream 100 bases:

>100_bases
ATGTTGTATTGGCTCAATGAACTCTCGGATGGCGGCGATGTCTTCAACCTGTTCCGGTACATCACGTTCCGAGCCGGGGG
CGCGTTCTTCACCGCGCTGA

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MILWTSADAASATGGTSQGTWTATGVSIDTRTIEPGDLFVALAAARDGHEFVAQAFQKGASAALVNHVPDGMDAAKCLVV
DDVLAALERLGTAARTRTQAKVIAITGSVGKTTVKDMLKQALSGQGKIHAAVASYNNHWGVPLTLSRMPVDTDYAVIEIG
MSAPGEIAPLSRMARPHVAMITTIAPAHLEAFGRIEGIAEEKASIFEGLEPDGVAIVPMDVETAPILARAAVAHAAEVVT
FGEAEGADLRLTDVMLSDDSTVMRIATDEGGRLAKLSVSGRHYAVNAVGVLCAVAASGADTTQAAMALSHWQPVEGRGQR
ERIQLSRTDDIAIELIDDAFNANPTSLAAGLEVLAAITPERGGRRIAVLGDMLELGDDGAKMHADIASLPAMEAVDIVHT
CGPLMVHLDAALPGAKRGEHTETAQDLCRAVPRTLRPGDVVLVKGSKGSRVSLVVDALRKLGQSLERAGQGNR

Sequences:

>Translated_473_residues
MILWTSADAASATGGTSQGTWTATGVSIDTRTIEPGDLFVALAAARDGHEFVAQAFQKGASAALVNHVPDGMDAAKCLVV
DDVLAALERLGTAARTRTQAKVIAITGSVGKTTVKDMLKQALSGQGKIHAAVASYNNHWGVPLTLSRMPVDTDYAVIEIG
MSAPGEIAPLSRMARPHVAMITTIAPAHLEAFGRIEGIAEEKASIFEGLEPDGVAIVPMDVETAPILARAAVAHAAEVVT
FGEAEGADLRLTDVMLSDDSTVMRIATDEGGRLAKLSVSGRHYAVNAVGVLCAVAASGADTTQAAMALSHWQPVEGRGQR
ERIQLSRTDDIAIELIDDAFNANPTSLAAGLEVLAAITPERGGRRIAVLGDMLELGDDGAKMHADIASLPAMEAVDIVHT
CGPLMVHLDAALPGAKRGEHTETAQDLCRAVPRTLRPGDVVLVKGSKGSRVSLVVDALRKLGQSLERAGQGNR
>Mature_473_residues
MILWTSADAASATGGTSQGTWTATGVSIDTRTIEPGDLFVALAAARDGHEFVAQAFQKGASAALVNHVPDGMDAAKCLVV
DDVLAALERLGTAARTRTQAKVIAITGSVGKTTVKDMLKQALSGQGKIHAAVASYNNHWGVPLTLSRMPVDTDYAVIEIG
MSAPGEIAPLSRMARPHVAMITTIAPAHLEAFGRIEGIAEEKASIFEGLEPDGVAIVPMDVETAPILARAAVAHAAEVVT
FGEAEGADLRLTDVMLSDDSTVMRIATDEGGRLAKLSVSGRHYAVNAVGVLCAVAASGADTTQAAMALSHWQPVEGRGQR
ERIQLSRTDDIAIELIDDAFNANPTSLAAGLEVLAAITPERGGRRIAVLGDMLELGDDGAKMHADIASLPAMEAVDIVHT
CGPLMVHLDAALPGAKRGEHTETAQDLCRAVPRTLRPGDVVLVKGSKGSRVSLVVDALRKLGQSLERAGQGNR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=445, Percent_Identity=34.3820224719101, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49261; Mature: 49261

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILWTSADAASATGGTSQGTWTATGVSIDTRTIEPGDLFVALAAARDGHEFVAQAFQKGA
CEEEECCCCCCCCCCCCCCEEEEECEEEECEECCCCCEEEEEEECCCCHHHHHHHHHCCC
SAALVNHVPDGMDAAKCLVVDDVLAALERLGTAARTRTQAKVIAITGSVGKTTVKDMLKQ
CEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEECCCCHHHHHHHHHH
ALSGQGKIHAAVASYNNHWGVPLTLSRMPVDTDYAVIEIGMSAPGEIAPLSRMARPHVAM
HHCCCCEEEEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHCCCCEEE
ITTIAPAHLEAFGRIEGIAEEKASIFEGLEPDGVAIVPMDVETAPILARAAVAHAAEVVT
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEE
FGEAEGADLRLTDVMLSDDSTVMRIATDEGGRLAKLSVSGRHYAVNAVGVLCAVAASGAD
ECCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEEEECCCEEEHHHHHHHHHHHCCCCC
TTQAAMALSHWQPVEGRGQRERIQLSRTDDIAIELIDDAFNANPTSLAAGLEVLAAITPE
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCCC
RGGRRIAVLGDMLELGDDGAKMHADIASLPAMEAVDIVHTCGPLMVHLDAALPGAKRGEH
CCCCEEEEEEHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCC
TETAQDLCRAVPRTLRPGDVVLVKGSKGSRVSLVVDALRKLGQSLERAGQGNR
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MILWTSADAASATGGTSQGTWTATGVSIDTRTIEPGDLFVALAAARDGHEFVAQAFQKGA
CEEEECCCCCCCCCCCCCCEEEEECEEEECEECCCCCEEEEEEECCCCHHHHHHHHHCCC
SAALVNHVPDGMDAAKCLVVDDVLAALERLGTAARTRTQAKVIAITGSVGKTTVKDMLKQ
CEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEEEECCCCHHHHHHHHHH
ALSGQGKIHAAVASYNNHWGVPLTLSRMPVDTDYAVIEIGMSAPGEIAPLSRMARPHVAM
HHCCCCEEEEEEEECCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHCCCCEEE
ITTIAPAHLEAFGRIEGIAEEKASIFEGLEPDGVAIVPMDVETAPILARAAVAHAAEVVT
EEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEE
FGEAEGADLRLTDVMLSDDSTVMRIATDEGGRLAKLSVSGRHYAVNAVGVLCAVAASGAD
ECCCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEEEECCCEEEHHHHHHHHHHHCCCCC
TTQAAMALSHWQPVEGRGQRERIQLSRTDDIAIELIDDAFNANPTSLAAGLEVLAAITPE
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCCC
RGGRRIAVLGDMLELGDDGAKMHADIASLPAMEAVDIVHTCGPLMVHLDAALPGAKRGEH
CCCCEEEEEEHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCC
TETAQDLCRAVPRTLRPGDVVLVKGSKGSRVSLVVDALRKLGQSLERAGQGNR
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]