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The map label for this gene is ilvE [H]

Identifier: 89053613

GI number: 89053613

Start: 1081884

End: 1082783

Strand: Reverse

Name: ilvE [H]

Synonym: Jann_1122

Alternate gene names: 89053613

Gene position: 1082783-1081884 (Counterclockwise)

Preceding gene: 89053614

Following gene: 89053612

Centisome position: 25.08

GC content: 66.0

Gene sequence:

>900_bases
ATGAATGATTTATCCAACGGCGCGGCCTGGATGGGGAGCCGCATCATCCCCATCGCAGAGGCCGCGATCCCCGTCACCGA
TTGGGGCCTGACCCATTCCGACATCGCCTATGACGTGGTGCCGGTCTGGCGGGGCGGCTTCTTCCGGCTGGATGATTACG
TGGCGCGGTTCATGGCCTCCGTCGGCGCGCTGCGGATGGATATCGGGCGCGACGGGGACGGCGTGAAAACGGCCCTGACC
CGGATGGTGGCCGCAAGCGGATTGCGCGACAGCTATGTCGCCATGGTCGCCGCGCGGGGGCGCAACCCGGTGCCCGGATC
GCGGGACCCGCGCGATTGTGCCAACCACTTCTACGCCTGGTGCGTGCCGTACGTGCACATCGTGAAACCCGAAATCGCCG
AGCGTGGAACCTCCGTCTGGATCGCCAAACGCACCCGCCGCATCCCCGCCGACAGCGTTGATCCCACCGTGAAAAACTAC
CATTGGGGCGATTTCACCGGCGGGTTGTTCGAGGCGAAAGACAAGGGGTTTGAGACGGTCTTGCTGCTGGACCACGCGGG
CCATGTGACCGAAGGGCCGGGCTTCAACGCCTTCGCCCTGTTCGGTGACCGGATCGTGACGTCGGACCACGGTGTGTTGC
ATGGCATCACCCGCCGCACGGTGCTGGAAATGGCCGCAGAGGCGGGCCTGACGGTCGAAACACGCCCGCTGCCCCTGGAC
GAATTCCTGGAGGCCGATGAGGTCTTCCTGTCGTCCTCGGGCGGCGGCGTGATCCCGGTGGCGCGGGTCGATAACCGCGT
CTTCTCCAACGATGCGGCAGGGCCCGTGGCGCTGGACCTGCGGCGGCGGTATTTCGACTGGATCACGCGGGCCGAGCACC
GAACCGACATCGCCTATTGA

Upstream 100 bases:

>100_bases
TCTGGCCGCCGCCGAGGACGTGAAGGGTGAGGTGCGCGCCTACGGGACCTGAGCGATTGGGGCACTTGAACCGCGCGCGG
GCCTGGGCCAGCGTCGGGGG

Downstream 100 bases:

>100_bases
GCGCCACGGGACACGGGCGTAGCGTCAGCGACAAGTACCACGGAGGTCCGCACCATGAACACCTACGCCCCCAATTCCTG
GGAAGCGCGCGCCGAGGAAT

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNDLSNGAAWMGSRIIPIAEAAIPVTDWGLTHSDIAYDVVPVWRGGFFRLDDYVARFMASVGALRMDIGRDGDGVKTALT
RMVAASGLRDSYVAMVAARGRNPVPGSRDPRDCANHFYAWCVPYVHIVKPEIAERGTSVWIAKRTRRIPADSVDPTVKNY
HWGDFTGGLFEAKDKGFETVLLLDHAGHVTEGPGFNAFALFGDRIVTSDHGVLHGITRRTVLEMAAEAGLTVETRPLPLD
EFLEADEVFLSSSGGGVIPVARVDNRVFSNDAAGPVALDLRRRYFDWITRAEHRTDIAY

Sequences:

>Translated_299_residues
MNDLSNGAAWMGSRIIPIAEAAIPVTDWGLTHSDIAYDVVPVWRGGFFRLDDYVARFMASVGALRMDIGRDGDGVKTALT
RMVAASGLRDSYVAMVAARGRNPVPGSRDPRDCANHFYAWCVPYVHIVKPEIAERGTSVWIAKRTRRIPADSVDPTVKNY
HWGDFTGGLFEAKDKGFETVLLLDHAGHVTEGPGFNAFALFGDRIVTSDHGVLHGITRRTVLEMAAEAGLTVETRPLPLD
EFLEADEVFLSSSGGGVIPVARVDNRVFSNDAAGPVALDLRRRYFDWITRAEHRTDIAY
>Mature_299_residues
MNDLSNGAAWMGSRIIPIAEAAIPVTDWGLTHSDIAYDVVPVWRGGFFRLDDYVARFMASVGALRMDIGRDGDGVKTALT
RMVAASGLRDSYVAMVAARGRNPVPGSRDPRDCANHFYAWCVPYVHIVKPEIAERGTSVWIAKRTRRIPADSVDPTVKNY
HWGDFTGGLFEAKDKGFETVLLLDHAGHVTEGPGFNAFALFGDRIVTSDHGVLHGITRRTVLEMAAEAGLTVETRPLPLD
EFLEADEVFLSSSGGGVIPVARVDNRVFSNDAAGPVALDLRRRYFDWITRAEHRTDIAY

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=247, Percent_Identity=23.8866396761134, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32861; Mature: 32861

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLSNGAAWMGSRIIPIAEAAIPVTDWGLTHSDIAYDVVPVWRGGFFRLDDYVARFMAS
CCCCCCCHHHHCCCCEEHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHH
VGALRMDIGRDGDGVKTALTRMVAASGLRDSYVAMVAARGRNPVPGSRDPRDCANHFYAW
HHHHEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
CVPYVHIVKPEIAERGTSVWIAKRTRRIPADSVDPTVKNYHWGDFTGGLFEAKDKGFETV
HHHHHHHCCCHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEE
LLLDHAGHVTEGPGFNAFALFGDRIVTSDHGVLHGITRRTVLEMAAEAGLTVETRPLPLD
EEEECCCCCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHH
EFLEADEVFLSSSGGGVIPVARVDNRVFSNDAAGPVALDLRRRYFDWITRAEHRTDIAY
HHHCHHHEEEECCCCCEEEEHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNDLSNGAAWMGSRIIPIAEAAIPVTDWGLTHSDIAYDVVPVWRGGFFRLDDYVARFMAS
CCCCCCCHHHHCCCCEEHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHH
VGALRMDIGRDGDGVKTALTRMVAASGLRDSYVAMVAARGRNPVPGSRDPRDCANHFYAW
HHHHEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
CVPYVHIVKPEIAERGTSVWIAKRTRRIPADSVDPTVKNYHWGDFTGGLFEAKDKGFETV
HHHHHHHCCCHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEE
LLLDHAGHVTEGPGFNAFALFGDRIVTSDHGVLHGITRRTVLEMAAEAGLTVETRPLPLD
EEEECCCCCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHH
EFLEADEVFLSSSGGGVIPVARVDNRVFSNDAAGPVALDLRRRYFDWITRAEHRTDIAY
HHHCHHHEEEECCCCCEEEEHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]