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The map label for this gene is lepB [H]

Identifier: 89053012

GI number: 89053012

Start: 513215

End: 513994

Strand: Direct

Name: lepB [H]

Synonym: Jann_0521

Alternate gene names: 89053012

Gene position: 513215-513994 (Clockwise)

Preceding gene: 89053011

Following gene: 89053013

Centisome position: 11.89

GC content: 61.41

Gene sequence:

>780_bases
ATGGCAGATTCTGAGGGCGGCATCATGGAGACGGTCAAGACCGTTGCCTGGGCGCTGGTGATCGCGGGAATATTCCGCAC
CTTGTTCTTTCAACCGTTCTGGATTCCATCGGGTTCGATGAAGGATACGTTGCTGATTGGCGATTTCCTGTTCGTCAACA
AGATGGCTTACGGCTATTCGCGCCATTCCTGCCCGTTTTCCATGTGCCCGTTCGAGGGGCGGATCTTCGGCTCGGACCCT
GAGCGGGGCGATGTGGTCGTCTTCCGGCATCCGACGAACGAGACCGATTTCATCAAACGTGTGATTGGCCTTCCGGGGGA
CCGCGTGCAGGTCATCGACGGGGTGCTACACCTGAACGGTGAGCCGGTGCGCCTGACCCCGGAGGAGCCGTTTGAAGAGA
TCGCCGAGGCGCAGGGTCCGCAAGGTCATGTTCCGCGCTGCGCCAATGCGCCTGTGGGCGAGGGCGGTGTCTGCTCCAAA
GAGCGGTTCACGGAAGAGCTTCCGGGCGGCGTCACCCATTCGATCCTGAATATCCAAGAGGGCACGCGGGGCGACAACAC
GCCGGAATTCGTGGTGCCCGAGGGGCATTTCTTCGTGATGGGGGACAATCGCGACAATTCCATCGACTCGCGGTTCCCGC
AATCCATCGGCGGCGTGGGCTTTGTGCCGGCGGAGAACCTGCTGGGTCGTGCGGACCGGGTGATTTTCTCGTCCGCGGGC
CAGCGAATGATCTACTTCTGGACCTGGCGCTCGGACCGCTTCTTCCGGGCGATCGAGTAG

Upstream 100 bases:

>100_bases
TGCGCAGCGACGCCCGGTTTGGGCCGTTTGCTTGACTTGGCCAATGGGTCGTGGGCAAGAACGCCTCTGAGGCAGTTGGA
TAACAAGGAAGGGCAGCCCA

Downstream 100 bases:

>100_bases
CCCGCGATGAAACCCGCGCGGGAGCTGACGGCCTTCATGGACCGCCTGGGGTATGTCTTTGAGGACCCCGCATTGCTCGC
GCGCGCGCTGACCCATTCGT

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MADSEGGIMETVKTVAWALVIAGIFRTLFFQPFWIPSGSMKDTLLIGDFLFVNKMAYGYSRHSCPFSMCPFEGRIFGSDP
ERGDVVVFRHPTNETDFIKRVIGLPGDRVQVIDGVLHLNGEPVRLTPEEPFEEIAEAQGPQGHVPRCANAPVGEGGVCSK
ERFTEELPGGVTHSILNIQEGTRGDNTPEFVVPEGHFFVMGDNRDNSIDSRFPQSIGGVGFVPAENLLGRADRVIFSSAG
QRMIYFWTWRSDRFFRAIE

Sequences:

>Translated_259_residues
MADSEGGIMETVKTVAWALVIAGIFRTLFFQPFWIPSGSMKDTLLIGDFLFVNKMAYGYSRHSCPFSMCPFEGRIFGSDP
ERGDVVVFRHPTNETDFIKRVIGLPGDRVQVIDGVLHLNGEPVRLTPEEPFEEIAEAQGPQGHVPRCANAPVGEGGVCSK
ERFTEELPGGVTHSILNIQEGTRGDNTPEFVVPEGHFFVMGDNRDNSIDSRFPQSIGGVGFVPAENLLGRADRVIFSSAG
QRMIYFWTWRSDRFFRAIE
>Mature_258_residues
ADSEGGIMETVKTVAWALVIAGIFRTLFFQPFWIPSGSMKDTLLIGDFLFVNKMAYGYSRHSCPFSMCPFEGRIFGSDPE
RGDVVVFRHPTNETDFIKRVIGLPGDRVQVIDGVLHLNGEPVRLTPEEPFEEIAEAQGPQGHVPRCANAPVGEGGVCSKE
RFTEELPGGVTHSILNIQEGTRGDNTPEFVVPEGHFFVMGDNRDNSIDSRFPQSIGGVGFVPAENLLGRADRVIFSSAGQ
RMIYFWTWRSDRFFRAIE

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=262, Percent_Identity=36.6412213740458, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 28792; Mature: 28661

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSEGGIMETVKTVAWALVIAGIFRTLFFQPFWIPSGSMKDTLLIGDFLFVNKMAYGYS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEHHHHHHHHHHHCCC
RHSCPFSMCPFEGRIFGSDPERGDVVVFRHPTNETDFIKRVIGLPGDRVQVIDGVLHLNG
CCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEHEEECCC
EPVRLTPEEPFEEIAEAQGPQGHVPRCANAPVGEGGVCSKERFTEELPGGVTHSILNIQE
CCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHEECCC
GTRGDNTPEFVVPEGHFFVMGDNRDNSIDSRFPQSIGGVGFVPAENLLGRADRVIFSSAG
CCCCCCCCCEEECCCEEEEEECCCCCCHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHCCC
QRMIYFWTWRSDRFFRAIE
CEEEEEEEECCCCEEEECC
>Mature Secondary Structure 
ADSEGGIMETVKTVAWALVIAGIFRTLFFQPFWIPSGSMKDTLLIGDFLFVNKMAYGYS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEHHHHHHHHHHHCCC
RHSCPFSMCPFEGRIFGSDPERGDVVVFRHPTNETDFIKRVIGLPGDRVQVIDGVLHLNG
CCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEHEEECCC
EPVRLTPEEPFEEIAEAQGPQGHVPRCANAPVGEGGVCSKERFTEELPGGVTHSILNIQE
CCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHEECCC
GTRGDNTPEFVVPEGHFFVMGDNRDNSIDSRFPQSIGGVGFVPAENLLGRADRVIFSSAG
CCCCCCCCCEEECCCEEEEEECCCCCCHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHCCC
QRMIYFWTWRSDRFFRAIE
CEEEEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]