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The map label for this gene is rhtB [H]

Identifier: 89052996

GI number: 89052996

Start: 501158

End: 501790

Strand: Reverse

Name: rhtB [H]

Synonym: Jann_0505

Alternate gene names: 89052996

Gene position: 501790-501158 (Counterclockwise)

Preceding gene: 89052997

Following gene: 89052995

Centisome position: 11.62

GC content: 61.14

Gene sequence:

>633_bases
ATGAACGGACCGCACGCTATGATCACCACCCAGTTCTTGCTTACTGCACTCGTCGTTGTCATCGCGCCCGGCACCGGCGT
CGTCTACACCCTCGCACTGGGTCTGGGACGAGGACGCAAAGCGGCGATCTGGGCGGCGCTCGGGTGCACGTTCGGCATCG
TCCCGCATCTTTTGGCCGCCACCCTTGGCTTGGCTGCAGTCCTCCACGCCTCCGCGCTTTTGTTCAATATTGTGAAATTC
TTAGGGGTAGCCTACCTGCTTTATCTCGCGGTGTCGGCCCTTCGGTCCGGCGGAGCGTTAAGCATCGAACCTGCCACCAC
CGTTGAGTCCGGCCTGACCGTCGCCAAACGCGGCGCGCTGATCAACATCCTCAATCCAAAACTCTCAATCTTCTTCCTGT
CCCTCCTCACACCGTTCCTGTCCGGAAACCCGGCAACGGTGACCCAGGAAATCGTCGTGCTCGGCGGCATCTTCATGGCC
ATGACGTTCGCGGTCTTTGTCCTCTACGGCCTCTTCGCAGCAACCGCGCGCACCTATATCCTTGGCTCCACACGTATCAT
GGCGTGGATGAACCGCTCCTTCGCGGCCATCTTCGCGCTCCTTGCCGGACGCCTCGCCCTGGAACGCGCCTAG

Upstream 100 bases:

>100_bases
CCGAACCGGCCCGGCCTTGGCGACAGCGACGGCGCGCCAATCCACTTGACTTGGGCAACGTCGAGGCGCACGCTATCCGT
AACCTAAATTACGGCACCCT

Downstream 100 bases:

>100_bases
CTCTTTCACCTTGGTAGAACACCTCCGGGGGGGCAAGGGGCAGCGCCCCCGTTGGGGTGGGAGGGCGGGCCCTCCCACCC
CCCAAAAAAACCCCCCTTGC

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNGPHAMITTQFLLTALVVVIAPGTGVVYTLALGLGRGRKAAIWAALGCTFGIVPHLLAATLGLAAVLHASALLFNIVKF
LGVAYLLYLAVSALRSGGALSIEPATTVESGLTVAKRGALINILNPKLSIFFLSLLTPFLSGNPATVTQEIVVLGGIFMA
MTFAVFVLYGLFAATARTYILGSTRIMAWMNRSFAAIFALLAGRLALERA

Sequences:

>Translated_210_residues
MNGPHAMITTQFLLTALVVVIAPGTGVVYTLALGLGRGRKAAIWAALGCTFGIVPHLLAATLGLAAVLHASALLFNIVKF
LGVAYLLYLAVSALRSGGALSIEPATTVESGLTVAKRGALINILNPKLSIFFLSLLTPFLSGNPATVTQEIVVLGGIFMA
MTFAVFVLYGLFAATARTYILGSTRIMAWMNRSFAAIFALLAGRLALERA
>Mature_210_residues
MNGPHAMITTQFLLTALVVVIAPGTGVVYTLALGLGRGRKAAIWAALGCTFGIVPHLLAATLGLAAVLHASALLFNIVKF
LGVAYLLYLAVSALRSGGALSIEPATTVESGLTVAKRGALINILNPKLSIFFLSLLTPFLSGNPATVTQEIVVLGGIFMA
MTFAVFVLYGLFAATARTYILGSTRIMAWMNRSFAAIFALLAGRLALERA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=26.7676767676768, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788099, Length=201, Percent_Identity=26.3681592039801, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21968; Mature: 21968

Theoretical pI: Translated: 10.96; Mature: 10.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPHAMITTQFLLTALVVVIAPGTGVVYTLALGLGRGRKAAIWAALGCTFGIVPHLLAA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TLGLAAVLHASALLFNIVKFLGVAYLLYLAVSALRSGGALSIEPATTVESGLTVAKRGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHCCCE
INILNPKLSIFFLSLLTPFLSGNPATVTQEIVVLGGIFMAMTFAVFVLYGLFAATARTYI
EEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSTRIMAWMNRSFAAIFALLAGRLALERA
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGPHAMITTQFLLTALVVVIAPGTGVVYTLALGLGRGRKAAIWAALGCTFGIVPHLLAA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
TLGLAAVLHASALLFNIVKFLGVAYLLYLAVSALRSGGALSIEPATTVESGLTVAKRGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHCCCE
INILNPKLSIFFLSLLTPFLSGNPATVTQEIVVLGGIFMAMTFAVFVLYGLFAATARTYI
EEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGSTRIMAWMNRSFAAIFALLAGRLALERA
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]