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The map label for this gene is gatA2 [H]

Identifier: 89052917

GI number: 89052917

Start: 428619

End: 429941

Strand: Direct

Name: gatA2 [H]

Synonym: Jann_0426

Alternate gene names: 89052917

Gene position: 428619-429941 (Clockwise)

Preceding gene: 89052915

Following gene: 89052918

Centisome position: 9.93

GC content: 64.1

Gene sequence:

>1323_bases
ATGGATTGGCTGAGCAAAAGCGCGAGTGAGATCGGGCGCGGTATCGGCGCGGGCGACGTGGACCCGGTGGCCCTGACCGA
AGGCTATCTGGACGTCATCAAGGCAAGCCCTTTTGGCGACCGCATTTACGCCCGCACCACCCGTGACCGGGCTTTGGCCG
AAGCGATTGCCGCCCATGACCGTGCCAAGGCTGGCGTGCGGCGCGGGTTGCTCGACGGTGTGCCGATCAGTTGGAAAGAC
CTGTTCGACACGGCTGGCGTCGTGACGGAGGCCGGGTCCAACCTGTTAGAGGGTCGCGTGCCCGATAAGGACGCTGAGGT
TTTGGCCAACGCCACCCGCGCGGGCCTTGTTTGCCTGGGCAAGACCCACATGACGGAACTCGCTTTTTCCGGCCTTGGGG
TGAATCCATCGACGGCCACACCCCCCAACGCGAACGACCCAGAGCTTGCGCCGGGCGGCTCGTCCTCAGGCGCAGCTACG
TCTGTGGCATACGGCATGGCGGCTGCGGGAATTGGGTCCGACACCGGTGGCTCGGTCCGCTTGCCGTCCGCGTGGAACGA
TCTGGTGGGGTTCAAGCCCTCCCACGGGGCGCTGTCTCTCGACGGGGTCGTGCCGCTGGCCGCCAGTTTTGACACGGTCG
GGCCGCTCTGCCGCACGGTTGAAGATTGCGCAGAACTCTTCGCTGTTATGGGCAACACCAAGGCGGCTGATTTGGCCGGG
GCGTCGCTGGACGGCACACGCCTGCTTGTTTTGAACCCCTACGCAAGTGATGTGCGCGATGCGCCCGGTGCGGCGTTCCA
ATCCGCTGTCGAACGGCTCAGCACCGCAGGCGCATTGATTGAAAACGCCGATATCCCCGCCGTTAATGAAGCGATGGACA
CGACGCTTTCTCTATATACCGCAGAAGCCTATGGCACCTGGGGTAAGGTGATTGAGGCCAACCCCGATAAGATGTTCCAC
CGCATTCGCGACCGCTTCCGCCAGGGTGCGGAAGTGAAGGCCGCTGATTTCGTGGCCCTCTGGCAGCGGCTGCGTGCCCT
GCGACAGGCGTATTACAAGGCGACCGCCAGCTATGACGCCGTGCTGATCCCCTCTGCCGCCAATCTACCGCCAAATGTCG
CGCGCCTGGACAGTGACGACGACTACTTCGTGACCGAAAACCTGCTGACCCTGCGCAATACCCGCGTTGGCAACCTGATG
GGCGTTTGCGCCGTGACCCTGCCCACCGGCGTGCCGTCCACCGGCCTTATGATGATGTGCTCCCCCGGTGACGACGCGCG
TTTGCTACGTCTGGCCCGTGCGGCGGAGGGCGCATTGGCCTAA

Upstream 100 bases:

>100_bases
CATCAAGCTGTTCCATAGCATGAACTATGGCGCGGCCATGGGGATTGTCCATGTGGCGCGTCGTCGTTAGCGTCCTTGGG
GGGCAGATAGGGGTGCACAG

Downstream 100 bases:

>100_bases
CCGCCACACCTCCCTCAGAACAGGGCGCTTTTTCTGGACCCGGAGTCTCTCGTTCGCTATCATCATCGCTAACAGGGCAT
GAATGACCCTGAATATGAGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MDWLSKSASEIGRGIGAGDVDPVALTEGYLDVIKASPFGDRIYARTTRDRALAEAIAAHDRAKAGVRRGLLDGVPISWKD
LFDTAGVVTEAGSNLLEGRVPDKDAEVLANATRAGLVCLGKTHMTELAFSGLGVNPSTATPPNANDPELAPGGSSSGAAT
SVAYGMAAAGIGSDTGGSVRLPSAWNDLVGFKPSHGALSLDGVVPLAASFDTVGPLCRTVEDCAELFAVMGNTKAADLAG
ASLDGTRLLVLNPYASDVRDAPGAAFQSAVERLSTAGALIENADIPAVNEAMDTTLSLYTAEAYGTWGKVIEANPDKMFH
RIRDRFRQGAEVKAADFVALWQRLRALRQAYYKATASYDAVLIPSAANLPPNVARLDSDDDYFVTENLLTLRNTRVGNLM
GVCAVTLPTGVPSTGLMMMCSPGDDARLLRLARAAEGALA

Sequences:

>Translated_440_residues
MDWLSKSASEIGRGIGAGDVDPVALTEGYLDVIKASPFGDRIYARTTRDRALAEAIAAHDRAKAGVRRGLLDGVPISWKD
LFDTAGVVTEAGSNLLEGRVPDKDAEVLANATRAGLVCLGKTHMTELAFSGLGVNPSTATPPNANDPELAPGGSSSGAAT
SVAYGMAAAGIGSDTGGSVRLPSAWNDLVGFKPSHGALSLDGVVPLAASFDTVGPLCRTVEDCAELFAVMGNTKAADLAG
ASLDGTRLLVLNPYASDVRDAPGAAFQSAVERLSTAGALIENADIPAVNEAMDTTLSLYTAEAYGTWGKVIEANPDKMFH
RIRDRFRQGAEVKAADFVALWQRLRALRQAYYKATASYDAVLIPSAANLPPNVARLDSDDDYFVTENLLTLRNTRVGNLM
GVCAVTLPTGVPSTGLMMMCSPGDDARLLRLARAAEGALA
>Mature_440_residues
MDWLSKSASEIGRGIGAGDVDPVALTEGYLDVIKASPFGDRIYARTTRDRALAEAIAAHDRAKAGVRRGLLDGVPISWKD
LFDTAGVVTEAGSNLLEGRVPDKDAEVLANATRAGLVCLGKTHMTELAFSGLGVNPSTATPPNANDPELAPGGSSSGAAT
SVAYGMAAAGIGSDTGGSVRLPSAWNDLVGFKPSHGALSLDGVVPLAASFDTVGPLCRTVEDCAELFAVMGNTKAADLAG
ASLDGTRLLVLNPYASDVRDAPGAAFQSAVERLSTAGALIENADIPAVNEAMDTTLSLYTAEAYGTWGKVIEANPDKMFH
RIRDRFRQGAEVKAADFVALWQRLRALRQAYYKATASYDAVLIPSAANLPPNVARLDSDDDYFVTENLLTLRNTRVGNLM
GVCAVTLPTGVPSTGLMMMCSPGDDARLLRLARAAEGALA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=241, Percent_Identity=36.5145228215768, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI195972892, Length=236, Percent_Identity=29.6610169491525, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=26.5356265356265, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=317, Percent_Identity=26.4984227129338, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319685, Length=364, Percent_Identity=27.7472527472527, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=291, Percent_Identity=30.2405498281787, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648113, Length=252, Percent_Identity=32.9365079365079, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648441, Length=397, Percent_Identity=23.4256926952141, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648439, Length=397, Percent_Identity=23.4256926952141, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648435, Length=397, Percent_Identity=23.4256926952141, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648437, Length=397, Percent_Identity=23.4256926952141, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI45550774, Length=397, Percent_Identity=23.4256926952141, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24652985, Length=250, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922090, Length=250, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652981, Length=250, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652983, Length=250, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 45885; Mature: 45885

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWLSKSASEIGRGIGAGDVDPVALTEGYLDVIKASPFGDRIYARTTRDRALAEAIAAHD
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
RAKAGVRRGLLDGVPISWKDLFDTAGVVTEAGSNLLEGRVPDKDAEVLANATRAGLVCLG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCEEEEC
KTHMTELAFSGLGVNPSTATPPNANDPELAPGGSSSGAATSVAYGMAAAGIGSDTGGSVR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEE
LPSAWNDLVGFKPSHGALSLDGVVPLAASFDTVGPLCRTVEDCAELFAVMGNTKAADLAG
CCCCHHHHCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ASLDGTRLLVLNPYASDVRDAPGAAFQSAVERLSTAGALIENADIPAVNEAMDTTLSLYT
CCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEE
AEAYGTWGKVIEANPDKMFHRIRDRFRQGAEVKAADFVALWQRLRALRQAYYKATASYDA
HHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VLIPSAANLPPNVARLDSDDDYFVTENLLTLRNTRVGNLMGVCAVTLPTGVPSTGLMMMC
EEECCCCCCCCCHHCCCCCCCEEEECCHHHHHCCCCHHHHHHHHEEECCCCCCCCEEEEE
SPGDDARLLRLARAAEGALA
CCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDWLSKSASEIGRGIGAGDVDPVALTEGYLDVIKASPFGDRIYARTTRDRALAEAIAAHD
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHH
RAKAGVRRGLLDGVPISWKDLFDTAGVVTEAGSNLLEGRVPDKDAEVLANATRAGLVCLG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCEEEEC
KTHMTELAFSGLGVNPSTATPPNANDPELAPGGSSSGAATSVAYGMAAAGIGSDTGGSVR
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEE
LPSAWNDLVGFKPSHGALSLDGVVPLAASFDTVGPLCRTVEDCAELFAVMGNTKAADLAG
CCCCHHHHCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
ASLDGTRLLVLNPYASDVRDAPGAAFQSAVERLSTAGALIENADIPAVNEAMDTTLSLYT
CCCCCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEE
AEAYGTWGKVIEANPDKMFHRIRDRFRQGAEVKAADFVALWQRLRALRQAYYKATASYDA
HHHHCCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
VLIPSAANLPPNVARLDSDDDYFVTENLLTLRNTRVGNLMGVCAVTLPTGVPSTGLMMMC
EEECCCCCCCCCHHCCCCCCCEEEECCHHHHHCCCCHHHHHHHHEEECCCCCCCCEEEEE
SPGDDARLLRLARAAEGALA
CCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]