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The map label for this gene is est [H]

Identifier: 89052878

GI number: 89052878

Start: 391364

End: 392284

Strand: Reverse

Name: est [H]

Synonym: Jann_0387

Alternate gene names: 89052878

Gene position: 392284-391364 (Counterclockwise)

Preceding gene: 89052879

Following gene: 89052876

Centisome position: 9.08

GC content: 66.45

Gene sequence:

>921_bases
ATGGGCGATACGGCCCCCTATCCCGGCCTCGCCCCGGACCTTTGGGATCTGGCGCGCGAGATGGCCGCCGACCCCGACCC
GGTGCCCGAGCATCTGCGCCCGATATCCCAGCTCCGCGCTGCGGTGGCGCGGATGGAGGCGGCGCTGCCACCCGTTCCTG
ACGATATTCAGACCCGCGACTGGACGATGGACGTGGCTGGCCGCGCGGTGCCCCTACGGAGCTATCAGACGGCAGACGCC
CGCGCAGCGGCGATGCTCTATATCCATGGTGGCGGGTGGAGCCTTGGGTCCATCCGAGGTCACGACCTCATCTGCGCAGA
TATCGCGCGGGACACTGGGCTGCGGGTGGTCAGCATCGATTACGCGTTGGCGCCGGAACATCCCTACCCGGCAGCGCTGA
CCGAATGTGTGGCGGTGTTTCAGCATCTTCGCACGGGCGCGTCACCTTTGGAGACGGTTCCGGGCCTCTGGCTTGGTGGG
GACAGCGCGGGCGGCAACCTTGCCCTTGGGGCCGCGCTGAAGACGAAAGGTGCGGCAGGGTTGTTCCTGATCTATCCGGC
AACGGACCCGACATGCGGTGCCGACAGCTACACAACCCATGCCAATGCCCCCTACCTCAGTCGCGACATGATGCACCGCT
GCTGGCGTGACTATCTGGGCGGGCAATCACCCGACATCTATACGAGCCCCAACACGGCAGACCTGCGGTCTTTGCCCCAG
GCCGTCATTCTGACGGCGACGCTCGACCCGCTCCTCGGTGACGGCGAAGAACTCGCCGCCACTTTCCTCGCGGCGGGCAC
ACCCGTCTGGTATGAGACGGCCTCCGGCCTCATCCATGGCTTTTTGCGCTTTCGCGCCATCTCGCCCGCCTCGGATGCCG
CCTTTCGGCGAGCCACTGCAGCCCTTGCCGTCCGCCTTTAA

Upstream 100 bases:

>100_bases
CACGCTTGGCAACCAGATGGTGTCCGAGCTGCTGATCAAGAACATCGCGCGGCGCGGGTATATTCTGGCGATCGACAAAG
ACGTCATCAAGATGATGTAG

Downstream 100 bases:

>100_bases
CGCAAAACGGGGCAGCCCTTGGCCACCCCGCTTCACGAGTCTCAATCAGTCGCGCCTCAGCAGTAAGGCGCACCGCGACC
GGCACCCGGCGCATCGGTCC

Product: alpha/beta hydrolase fold-3 protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MGDTAPYPGLAPDLWDLAREMAADPDPVPEHLRPISQLRAAVARMEAALPPVPDDIQTRDWTMDVAGRAVPLRSYQTADA
RAAAMLYIHGGGWSLGSIRGHDLICADIARDTGLRVVSIDYALAPEHPYPAALTECVAVFQHLRTGASPLETVPGLWLGG
DSAGGNLALGAALKTKGAAGLFLIYPATDPTCGADSYTTHANAPYLSRDMMHRCWRDYLGGQSPDIYTSPNTADLRSLPQ
AVILTATLDPLLGDGEELAATFLAAGTPVWYETASGLIHGFLRFRAISPASDAAFRRATAALAVRL

Sequences:

>Translated_306_residues
MGDTAPYPGLAPDLWDLAREMAADPDPVPEHLRPISQLRAAVARMEAALPPVPDDIQTRDWTMDVAGRAVPLRSYQTADA
RAAAMLYIHGGGWSLGSIRGHDLICADIARDTGLRVVSIDYALAPEHPYPAALTECVAVFQHLRTGASPLETVPGLWLGG
DSAGGNLALGAALKTKGAAGLFLIYPATDPTCGADSYTTHANAPYLSRDMMHRCWRDYLGGQSPDIYTSPNTADLRSLPQ
AVILTATLDPLLGDGEELAATFLAAGTPVWYETASGLIHGFLRFRAISPASDAAFRRATAALAVRL
>Mature_305_residues
GDTAPYPGLAPDLWDLAREMAADPDPVPEHLRPISQLRAAVARMEAALPPVPDDIQTRDWTMDVAGRAVPLRSYQTADAR
AAAMLYIHGGGWSLGSIRGHDLICADIARDTGLRVVSIDYALAPEHPYPAALTECVAVFQHLRTGASPLETVPGLWLGGD
SAGGNLALGAALKTKGAAGLFLIYPATDPTCGADSYTTHANAPYLSRDMMHRCWRDYLGGQSPDIYTSPNTADLRSLPQA
VILTATLDPLLGDGEELAATFLAAGTPVWYETASGLIHGFLRFRAISPASDAAFRRATAALAVRL

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68051721, Length=153, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI21328446, Length=169, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI68299767, Length=194, Percent_Identity=28.8659793814433, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=328, Percent_Identity=27.1341463414634, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17567059, Length=263, Percent_Identity=28.8973384030418, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71996133, Length=143, Percent_Identity=31.4685314685315, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI72001146, Length=134, Percent_Identity=34.3283582089552, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656084, Length=170, Percent_Identity=30, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24656076, Length=170, Percent_Identity=30, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI20130169, Length=170, Percent_Identity=30, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 32499; Mature: 32368

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDTAPYPGLAPDLWDLAREMAADPDPVPEHLRPISQLRAAVARMEAALPPVPDDIQTRD
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
WTMDVAGRAVPLRSYQTADARAAAMLYIHGGGWSLGSIRGHDLICADIARDTGLRVVSID
EEEECCCCEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEHHCCCCCEEEEEE
YALAPEHPYPAALTECVAVFQHLRTGASPLETVPGLWLGGDSAGGNLALGAALKTKGAAG
EEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCCEEEEHHHHCCCCCC
LFLIYPATDPTCGADSYTTHANAPYLSRDMMHRCWRDYLGGQSPDIYTSPNTADLRSLPQ
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHCCH
AVILTATLDPLLGDGEELAATFLAAGTPVWYETASGLIHGFLRFRAISPASDAAFRRATA
HEEEEEHHCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALAVRL
HHEECC
>Mature Secondary Structure 
GDTAPYPGLAPDLWDLAREMAADPDPVPEHLRPISQLRAAVARMEAALPPVPDDIQTRD
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
WTMDVAGRAVPLRSYQTADARAAAMLYIHGGGWSLGSIRGHDLICADIARDTGLRVVSID
EEEECCCCEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEHHCCCCCEEEEEE
YALAPEHPYPAALTECVAVFQHLRTGASPLETVPGLWLGGDSAGGNLALGAALKTKGAAG
EEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCCCCCEEEEHHHHCCCCCC
LFLIYPATDPTCGADSYTTHANAPYLSRDMMHRCWRDYLGGQSPDIYTSPNTADLRSLPQ
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHCCH
AVILTATLDPLLGDGEELAATFLAAGTPVWYETASGLIHGFLRFRAISPASDAAFRRATA
HEEEEEHHCHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
ALAVRL
HHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]