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The map label for this gene is mepA

Identifier: 89052835

GI number: 89052835

Start: 349068

End: 349970

Strand: Direct

Name: mepA

Synonym: Jann_0344

Alternate gene names: 89052835

Gene position: 349068-349970 (Clockwise)

Preceding gene: 89052834

Following gene: 89052836

Centisome position: 8.08

GC content: 62.46

Gene sequence:

>903_bases
ATGAAACGCCTCTTTCCCTTTACATTGATGGTCCTCGCGGCCTGTAACACCGGCGGGCCGACCGTCCAGCTAGATGCCAC
CTTGTCGGGCAGTGAAGCGCGGCAATATTTCGGCGCGGAAACGCGGGGGTCCGGCGGGGCGAGTGAGGCGATTGGCTTCT
ATTCAAACGGGTGCCTTGCTGGCGGTGTCGAATTGCCGGAAACCGGCCCGACCTGGCAAGCCATGCGGCTGAGCCGGGAC
CGGAACTGGGGCCATACCGAACTGATCGACTATATCGAAACGCTGACGGCCAATGTCGCGCGCAACACGTCGTGGAACGG
GCTCTATGTCGGCGATATGGCGCAGCCGCGCGGTGGGCCGATGCTGACGGGCCACGCAAGTCATCAAAGCGGCATTGATG
TTGATATGTGGATGTTGCCAGCCCGCGATCTGACCCTGACGGAGGAGGAGCGGGAGAACCTGTCCTCCATTTCCGTGCGC
GCGAACCGAGGCGCTACGGTCAACGAAAACTGGACTGCGGATCATGCGGAGGTGCTGCGATTGGCGGCTAGTGATCCCCG
GGTGGCGCGGATATTTGTCACCACTGGCGTGAAGGTCTGGCTGTGTCAGAATGTGGAGGGCGACCGCAGTTGGCTGTCGC
ACATCCGGCCCGCTAACGGGCACCACTACCATTTCCACGTGCGGCTCAATTGCCCTTCAGGCGACCGCAATTGTCAGAAC
CAACCATTGCCCCAGGGCGATGGATGTCAGGAAGCCTATGACCGCGCCGCCCGGATTATCAACCCGCCGCCCCCCGGCCC
GCCCAACAATGATCCGCCCGTCGCCTCGCCACCGCGCGGAATGCGGGTCGCTTTGGGCGATATGCCCAACCAATGCGTCG
GTGTGTTGAGCGGGTTGCAATAG

Upstream 100 bases:

>100_bases
CGCAGGGTCGCAAGACGAATGACTGATCCCGCAGGGTCGCAATGCAAATGATTGATCCCGCAGGGTCGCGATACATTGAA
TTTCAGTTTGGAGACGCATT

Downstream 100 bases:

>100_bases
GCGGCCACGCCTGCGCACCCGGCGCCCGGATCACGTATCGCCGGAAGGTACGGAATCCATGCCTGCCAGCAAGCCGGTCG
GAGTGGTATTGAGCTCAAAT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKRLFPFTLMVLAACNTGGPTVQLDATLSGSEARQYFGAETRGSGGASEAIGFYSNGCLAGGVELPETGPTWQAMRLSRD
RNWGHTELIDYIETLTANVARNTSWNGLYVGDMAQPRGGPMLTGHASHQSGIDVDMWMLPARDLTLTEEERENLSSISVR
ANRGATVNENWTADHAEVLRLAASDPRVARIFVTTGVKVWLCQNVEGDRSWLSHIRPANGHHYHFHVRLNCPSGDRNCQN
QPLPQGDGCQEAYDRAARIINPPPPGPPNNDPPVASPPRGMRVALGDMPNQCVGVLSGLQ

Sequences:

>Translated_300_residues
MKRLFPFTLMVLAACNTGGPTVQLDATLSGSEARQYFGAETRGSGGASEAIGFYSNGCLAGGVELPETGPTWQAMRLSRD
RNWGHTELIDYIETLTANVARNTSWNGLYVGDMAQPRGGPMLTGHASHQSGIDVDMWMLPARDLTLTEEERENLSSISVR
ANRGATVNENWTADHAEVLRLAASDPRVARIFVTTGVKVWLCQNVEGDRSWLSHIRPANGHHYHFHVRLNCPSGDRNCQN
QPLPQGDGCQEAYDRAARIINPPPPGPPNNDPPVASPPRGMRVALGDMPNQCVGVLSGLQ
>Mature_300_residues
MKRLFPFTLMVLAACNTGGPTVQLDATLSGSEARQYFGAETRGSGGASEAIGFYSNGCLAGGVELPETGPTWQAMRLSRD
RNWGHTELIDYIETLTANVARNTSWNGLYVGDMAQPRGGPMLTGHASHQSGIDVDMWMLPARDLTLTEEERENLSSISVR
ANRGATVNENWTADHAEVLRLAASDPRVARIFVTTGVKVWLCQNVEGDRSWLSHIRPANGHHYHFHVRLNCPSGDRNCQN
QPLPQGDGCQEAYDRAARIINPPPPGPPNNDPPVASPPRGMRVALGDMPNQCVGVLSGLQ

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=237, Percent_Identity=36.7088607594937, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 32578; Mature: 32578

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLFPFTLMVLAACNTGGPTVQLDATLSGSEARQYFGAETRGSGGASEAIGFYSNGCLA
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHCCCCCCCCCCCHHCCEEECCCEEE
GGVELPETGPTWQAMRLSRDRNWGHTELIDYIETLTANVARNTSWNGLYVGDMAQPRGGP
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
MLTGHASHQSGIDVDMWMLPARDLTLTEEERENLSSISVRANRGATVNENWTADHAEVLR
EEEECCCCCCCCCEEEEEECCCCCEECHHHHHCHHEEEEECCCCCCCCCCCCCCHHHHHH
LAASDPRVARIFVTTGVKVWLCQNVEGDRSWLSHIRPANGHHYHFHVRLNCPSGDRNCQN
HHCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCC
QPLPQGDGCQEAYDRAARIINPPPPGPPNNDPPVASPPRGMRVALGDMPNQCVGVLSGLQ
CCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MKRLFPFTLMVLAACNTGGPTVQLDATLSGSEARQYFGAETRGSGGASEAIGFYSNGCLA
CCCCHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHCCCCCCCCCCCHHCCEEECCCEEE
GGVELPETGPTWQAMRLSRDRNWGHTELIDYIETLTANVARNTSWNGLYVGDMAQPRGGP
CCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
MLTGHASHQSGIDVDMWMLPARDLTLTEEERENLSSISVRANRGATVNENWTADHAEVLR
EEEECCCCCCCCCEEEEEECCCCCEECHHHHHCHHEEEEECCCCCCCCCCCCCCHHHHHH
LAASDPRVARIFVTTGVKVWLCQNVEGDRSWLSHIRPANGHHYHFHVRLNCPSGDRNCQN
HHCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCC
QPLPQGDGCQEAYDRAARIINPPPPGPPNNDPPVASPPRGMRVALGDMPNQCVGVLSGLQ
CCCCCCCCHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]