Definition Ehrlichia chaffeensis str. Arkansas, complete genome.
Accession NC_007799
Length 1,176,248

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The map label for this gene is hisS

Identifier: 88658069

GI number: 88658069

Start: 273511

End: 274752

Strand: Direct

Name: hisS

Synonym: ECH_0291

Alternate gene names: 88658069

Gene position: 273511-274752 (Clockwise)

Preceding gene: 88657946

Following gene: 88657598

Centisome position: 23.25

GC content: 30.92

Gene sequence:

>1242_bases
ATGCAGCGTGATAAGTTAAGAGAAGTAAGGGGTACCAAGGATCTATTAGGGATTGAACTTTATAAGTTTCAATATATACA
TCGTTTATCTCAGTCAATAGCGCATAGATATGGATTTATAGCTGTTGATACTCCAATCATTGAATTTACTGAAGTGTTTA
CTAAGACTTTAGGTGATGATTCAGATATTGTAATGAAGGAAATGTATAATTTTCAGGATAAAAGTGGTGAAAATATTACA
TTAAGGCCAGAATTTACATCTGCTATTGTGAGGGTATTAATTAATAAAAATTTAATTATTCCTGTCAAATTATTTTCTAG
TGGACCAGTATTTCGTTATGAACGTCCTCAAAAGTGTCGTCAAAGACAATTTCATCAAGTTAATTTTGAGTTTTTTGGAA
GTGATCTTCCTCTTGCAGATATTGAAATGATAGCATTAGGCTATCATATTTTGGATGAGTTGAAGTTGTTAGATGATATA
ACGTTAGAGATTAATTTTCTAGGTGATAAGGAAACTATGAACTCATATAAGCTCAGTCTTATAGAGTATTTAAACAAATA
TAAGAAAGATTTATCAGAAGATAGTCAGCGTAGGCTTATTACAAATCCTCTAAGAATATTAGATTCAAAAGCTCCAGAAG
ATCGTGAAATTTTATTGAATGTTCCTAATATTTCTTATTTTTATAGTAAATCCTCTAATGATTTTTTTGCAGAGGTTCTA
TATGGGTTAGATGAGCTCTGTATTCCTTATAAAGTAAATCATAGTATAGTTAGAGGTTTAGATTATTATTGTAATACGGT
GTTTGAGTTTACAACGTCTAAATTAGGAGCACAAAATGCAGTTGTTGCTGGTGGTAGATATGATGGATTAGTGAAGTCTA
TGGGAGGAAATGATACTCCTGCTGTTGGTTTTGCTATGGGTGTTGAAAGAGTTAGTGCGTTGATAGATTATAAACATCAA
GAACCTAGGAGTGTAGTATTGATACCTATAGGGAAAGATGCAGTATCCTATGCTTTGAAGCTTGCTTATGAGTTACGTTG
TAATGGTATTTCTGTAAACTGGAATTATAAAAATGCTAATTTGAGAAATGCATTAAGGAAAGTAGGTGATGATAATATAG
TGTTAATTTTTGGAGATGAAGAGCTAAAAAATAATACTATACAAGTCAAAGATATGAAAACTGGTGAACAACAAGAAATA
GTTAGATGTGATTTATTGGACACATTGTGTAACAAAATTTAA

Upstream 100 bases:

>100_bases
AGTGTTATCATTATTCTAGAATATTTAAAATGTGTGTTAATTAATATGGTAATATGTAGTTCGAGTATAATACGAGTAGA
TTGATGTAAGGTAGAGTTTT

Downstream 100 bases:

>100_bases
CAGTGTTTGTCAAGTTATGAAAAAAGCGTAAGGATTTAGTTAAAAAATTTCTATTTTTATTTAAATGTGTTATTATTTAA
AGTACATCTGAAAAAGAAGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MQRDKLREVRGTKDLLGIELYKFQYIHRLSQSIAHRYGFIAVDTPIIEFTEVFTKTLGDDSDIVMKEMYNFQDKSGENIT
LRPEFTSAIVRVLINKNLIIPVKLFSSGPVFRYERPQKCRQRQFHQVNFEFFGSDLPLADIEMIALGYHILDELKLLDDI
TLEINFLGDKETMNSYKLSLIEYLNKYKKDLSEDSQRRLITNPLRILDSKAPEDREILLNVPNISYFYSKSSNDFFAEVL
YGLDELCIPYKVNHSIVRGLDYYCNTVFEFTTSKLGAQNAVVAGGRYDGLVKSMGGNDTPAVGFAMGVERVSALIDYKHQ
EPRSVVLIPIGKDAVSYALKLAYELRCNGISVNWNYKNANLRNALRKVGDDNIVLIFGDEELKNNTIQVKDMKTGEQQEI
VRCDLLDTLCNKI

Sequences:

>Translated_413_residues
MQRDKLREVRGTKDLLGIELYKFQYIHRLSQSIAHRYGFIAVDTPIIEFTEVFTKTLGDDSDIVMKEMYNFQDKSGENIT
LRPEFTSAIVRVLINKNLIIPVKLFSSGPVFRYERPQKCRQRQFHQVNFEFFGSDLPLADIEMIALGYHILDELKLLDDI
TLEINFLGDKETMNSYKLSLIEYLNKYKKDLSEDSQRRLITNPLRILDSKAPEDREILLNVPNISYFYSKSSNDFFAEVL
YGLDELCIPYKVNHSIVRGLDYYCNTVFEFTTSKLGAQNAVVAGGRYDGLVKSMGGNDTPAVGFAMGVERVSALIDYKHQ
EPRSVVLIPIGKDAVSYALKLAYELRCNGISVNWNYKNANLRNALRKVGDDNIVLIFGDEELKNNTIQVKDMKTGEQQEI
VRCDLLDTLCNKI
>Mature_413_residues
MQRDKLREVRGTKDLLGIELYKFQYIHRLSQSIAHRYGFIAVDTPIIEFTEVFTKTLGDDSDIVMKEMYNFQDKSGENIT
LRPEFTSAIVRVLINKNLIIPVKLFSSGPVFRYERPQKCRQRQFHQVNFEFFGSDLPLADIEMIALGYHILDELKLLDDI
TLEINFLGDKETMNSYKLSLIEYLNKYKKDLSEDSQRRLITNPLRILDSKAPEDREILLNVPNISYFYSKSSNDFFAEVL
YGLDELCIPYKVNHSIVRGLDYYCNTVFEFTTSKLGAQNAVVAGGRYDGLVKSMGGNDTPAVGFAMGVERVSALIDYKHQ
EPRSVVLIPIGKDAVSYALKLAYELRCNGISVNWNYKNANLRNALRKVGDDNIVLIFGDEELKNNTIQVKDMKTGEQQEI
VRCDLLDTLCNKI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=452, Percent_Identity=27.212389380531, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI6996014, Length=466, Percent_Identity=25.3218884120172, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1788861, Length=408, Percent_Identity=38.4803921568627, Blast_Score=298, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI71993686, Length=423, Percent_Identity=24.5862884160757, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71993693, Length=423, Percent_Identity=24.5862884160757, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325290, Length=467, Percent_Identity=26.338329764454, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24643061, Length=468, Percent_Identity=24.7863247863248, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_EHRCR (Q2GHH1)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507112.1
- ProteinModelPortal:   Q2GHH1
- SMR:   Q2GHH1
- STRING:   Q2GHH1
- GeneID:   3927256
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0291
- TIGR:   ECH_0291
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q2GHH1
- ProtClustDB:   PRK00037
- BioCyc:   ECHA205920:ECH_0291-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47347; Mature: 47347

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MQRDKLREVRGTKDLLGIELYKFQYIHRLSQSIAHRYGFIAVDTPIIEFTEVFTKTLGDD
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCC
SDIVMKEMYNFQDKSGENITLRPEFTSAIVRVLINKNLIIPVKLFSSGPVFRYERPQKCR
CHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCHHHHH
QRQFHQVNFEFFGSDLPLADIEMIALGYHILDELKLLDDITLEINFLGDKETMNSYKLSL
HHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHH
IEYLNKYKKDLSEDSQRRLITNPLRILDSKAPEDREILLNVPNISYFYSKSSNDFFAEVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHH
YGLDELCIPYKVNHSIVRGLDYYCNTVFEFTTSKLGAQNAVVAGGRYDGLVKSMGGNDTP
HCHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCC
AVGFAMGVERVSALIDYKHQEPRSVVLIPIGKDAVSYALKLAYELRCNGISVNWNYKNAN
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEEECEEEEEEEECCCH
LRNALRKVGDDNIVLIFGDEELKNNTIQVKDMKTGEQQEIVRCDLLDTLCNKI
HHHHHHHCCCCCEEEEECCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRDKLREVRGTKDLLGIELYKFQYIHRLSQSIAHRYGFIAVDTPIIEFTEVFTKTLGDD
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHCCCC
SDIVMKEMYNFQDKSGENITLRPEFTSAIVRVLINKNLIIPVKLFSSGPVFRYERPQKCR
CHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCHHHHH
QRQFHQVNFEFFGSDLPLADIEMIALGYHILDELKLLDDITLEINFLGDKETMNSYKLSL
HHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHH
IEYLNKYKKDLSEDSQRRLITNPLRILDSKAPEDREILLNVPNISYFYSKSSNDFFAEVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHH
YGLDELCIPYKVNHSIVRGLDYYCNTVFEFTTSKLGAQNAVVAGGRYDGLVKSMGGNDTP
HCHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCCCC
AVGFAMGVERVSALIDYKHQEPRSVVLIPIGKDAVSYALKLAYELRCNGISVNWNYKNAN
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHEEEECEEEEEEEECCCH
LRNALRKVGDDNIVLIFGDEELKNNTIQVKDMKTGEQQEIVRCDLLDTLCNKI
HHHHHHHCCCCCEEEEECCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA