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The map label for this gene is dut

Identifier: 88608458

GI number: 88608458

Start: 841754

End: 842203

Strand: Direct

Name: dut

Synonym: NSE_0957

Alternate gene names: 88608458

Gene position: 841754-842203 (Clockwise)

Preceding gene: 88608846

Following gene: 88608652

Centisome position: 97.99

GC content: 44.22

Gene sequence:

>450_bases
ATGCAAGAGCACGCAAAGATACTTCTTTTGCCACATGGTAAGGATTTGGAAATACCAAGATATGCAAGTAAGGGAAGTTC
AGGATTGGACCTTCGTGCGGCCATAGAAGGAAGCATGACCTTGAAACCTGGAATGTTCGAGCTGGTACCCACGGGCATCT
GCATCGAACTACCCGAGGGATTAGAAGCGCAAATAAGGCCACGCTCAGGTCTCGCAGCAAAGTTTGGTATAACGGTTCTT
AATAGTCCTGGCACTGTAGATCAGGACTATAGGGGAGAAATAAAAGTCTGTTTAGTAAATCTGTCTAAGAATGAATTTAC
TATCAACAGGGGCGACAGGATAGCTCAAATGGTAATAGCAAAAGTAGAACAAATACTGCTAGTGGAAGCAGAAGAAATTG
GGGAAACAGAGAGAGCTTCGGGGAGTTTTGGATCAACTGGAACTGAGTAA

Upstream 100 bases:

>100_bases
TTTCTATTCGGTAGCACTTCAATCGTGCTCATTTCGCCGCAAAATTTACATTCGGTGACATACTTGTTAGAGTGAGGGGA
ACCTAGGAGAAAAAATCTAC

Downstream 100 bases:

>100_bases
GGTTGGGTGTGGCCAAAACTAGCAGTGAGTCCAGATTATCTCAAAGGCTAGATAGATTCGTTAAGTAAGCAACTAAAAGA
AGAGACTGATCGAATTTGAA

Product: deoxyuridine 5'triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MQEHAKILLLPHGKDLEIPRYASKGSSGLDLRAAIEGSMTLKPGMFELVPTGICIELPEGLEAQIRPRSGLAAKFGITVL
NSPGTVDQDYRGEIKVCLVNLSKNEFTINRGDRIAQMVIAKVEQILLVEAEEIGETERASGSFGSTGTE

Sequences:

>Translated_149_residues
MQEHAKILLLPHGKDLEIPRYASKGSSGLDLRAAIEGSMTLKPGMFELVPTGICIELPEGLEAQIRPRSGLAAKFGITVL
NSPGTVDQDYRGEIKVCLVNLSKNEFTINRGDRIAQMVIAKVEQILLVEAEEIGETERASGSFGSTGTE
>Mature_149_residues
MQEHAKILLLPHGKDLEIPRYASKGSSGLDLRAAIEGSMTLKPGMFELVPTGICIELPEGLEAQIRPRSGLAAKFGITVL
NSPGTVDQDYRGEIKVCLVNLSKNEFTINRGDRIAQMVIAKVEQILLVEAEEIGETERASGSFGSTGTE

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=129, Percent_Identity=41.0852713178295, Blast_Score=95, Evalue=2e-20,
Organism=Homo sapiens, GI4503423, Length=129, Percent_Identity=41.0852713178295, Blast_Score=94, Evalue=3e-20,
Organism=Homo sapiens, GI70906441, Length=129, Percent_Identity=41.0852713178295, Blast_Score=94, Evalue=6e-20,
Organism=Escherichia coli, GI1790071, Length=145, Percent_Identity=41.3793103448276, Blast_Score=112, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71988561, Length=134, Percent_Identity=41.7910447761194, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=40.2985074626866, Blast_Score=92, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24583610, Length=131, Percent_Identity=37.4045801526718, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=37.4045801526718, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_NEOSM (Q2GCH5)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506821.1
- ProteinModelPortal:   Q2GCH5
- SMR:   Q2GCH5
- STRING:   Q2GCH5
- GeneID:   3931824
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0957
- TIGR:   NSE_0957
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- PhylomeDB:   Q2GCH5
- ProtClustDB:   CLSK2527931
- BioCyc:   NSEN222891:NSE_0957-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16081; Mature: 16081

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: BINDING 81-81 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEHAKILLLPHGKDLEIPRYASKGSSGLDLRAAIEGSMTLKPGMFELVPTGICIELPEG
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCEEEECCCEEEECCCC
LEAQIRPRSGLAAKFGITVLNSPGTVDQDYRGEIKVCLVNLSKNEFTINRGDRIAQMVIA
CCEEECCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEECCCEEEECCCHHHHHHHHH
KVEQILLVEAEEIGETERASGSFGSTGTE
HHHHHEEEEHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQEHAKILLLPHGKDLEIPRYASKGSSGLDLRAAIEGSMTLKPGMFELVPTGICIELPEG
CCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCCEEEECCCEEEECCCC
LEAQIRPRSGLAAKFGITVLNSPGTVDQDYRGEIKVCLVNLSKNEFTINRGDRIAQMVIA
CCEEECCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEECCCEEEECCCHHHHHHHHH
KVEQILLVEAEEIGETERASGSFGSTGTE
HHHHHEEEEHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA