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The map label for this gene is thiDE [H]

Identifier: 88608846

GI number: 88608846

Start: 839095

End: 839865

Strand: Direct

Name: thiDE [H]

Synonym: NSE_0954

Alternate gene names: 88608846

Gene position: 839095-839865 (Clockwise)

Preceding gene: 88608175

Following gene: 88608458

Centisome position: 97.68

GC content: 42.15

Gene sequence:

>771_bases
ATGCGTAAGATACCGGAAGTCTGCATCGTAGCGGGTTCAGATTCTATAGGTGGAGCTGGACTACAAGCAGATCTTAGGAT
CGCTGCATTACTAGAGTGTAGTGCATCGAATGTTGTCACGTGCATTACAGCCCAGGACCTGAGAGGGGTACATGCAATTC
AGTATTTATCTGGGGAAATCGTATCGAAGCAATTAATCTGTGCACTCTCTAGGAAACCTAAGGCGGTGAAAATAGGGATG
TTGGGTAATAGCGAAATAGCACTGGTAGTCTATGATGTTTTAAAGGATGCAGGAATCCCAATTGTATTAGATCCTGTCTT
GATATCTACTTCAAATTCCAAGCTCGCTGATGAAGGTGCAGTGCAAGTGCTACTAAAAAAGCTTATTTCTATCTCACACT
TGGTTACTCCCAACCTCTTTGAAGCAGGCGTCCTATCAGGAAGTAAAGTTTTTGATGCTGAATCTGCAAAAATTGCCGCG
TTGAGAATCCTCGAACTAGGAGCAAAAAACGTTCTTGTAAAAGGAATCCACATTAAAGAAGGGAAAATCATGGATTTGCT
CATTTCACAGGAAGGCACCACCATGGAATTTCAGAATGAAGTAATACGGATAAATATAACTCATGGCTCTGGATGTAGAT
TATCCACAGCGATTGCATGCCATATGTCGATGGGCAAATCCTTAAGGGAGTCAATTCATCTTGCTTCGGATCTCCTAAGG
AATGAGATCATCCGCATGGGAGTTTTATACAGTACTGATACTTGGTTGTAG

Upstream 100 bases:

>100_bases
GCTAGCCTGCGGAAGGCAGGGTCTGGCTACCATAGCAAATGAGATTTATGCAACCAGAAAGGCAGTAAGAGGAGCGTAGA
AATGATATTCTGTAAGGTGT

Downstream 100 bases:

>100_bases
GGGCTTGCTCACGTGCCCTTTGCTTCCATAATGTCATATCTAGTCACCGAAGCTGCCAATGCTAGCGCATCATACAAATC
TGAGTATTTTGATAAGCAAG

Product: putative phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRKIPEVCIVAGSDSIGGAGLQADLRIAALLECSASNVVTCITAQDLRGVHAIQYLSGEIVSKQLICALSRKPKAVKIGM
LGNSEIALVVYDVLKDAGIPIVLDPVLISTSNSKLADEGAVQVLLKKLISISHLVTPNLFEAGVLSGSKVFDAESAKIAA
LRILELGAKNVLVKGIHIKEGKIMDLLISQEGTTMEFQNEVIRINITHGSGCRLSTAIACHMSMGKSLRESIHLASDLLR
NEIIRMGVLYSTDTWL

Sequences:

>Translated_256_residues
MRKIPEVCIVAGSDSIGGAGLQADLRIAALLECSASNVVTCITAQDLRGVHAIQYLSGEIVSKQLICALSRKPKAVKIGM
LGNSEIALVVYDVLKDAGIPIVLDPVLISTSNSKLADEGAVQVLLKKLISISHLVTPNLFEAGVLSGSKVFDAESAKIAA
LRILELGAKNVLVKGIHIKEGKIMDLLISQEGTTMEFQNEVIRINITHGSGCRLSTAIACHMSMGKSLRESIHLASDLLR
NEIIRMGVLYSTDTWL
>Mature_256_residues
MRKIPEVCIVAGSDSIGGAGLQADLRIAALLECSASNVVTCITAQDLRGVHAIQYLSGEIVSKQLICALSRKPKAVKIGM
LGNSEIALVVYDVLKDAGIPIVLDPVLISTSNSKLADEGAVQVLLKKLISISHLVTPNLFEAGVLSGSKVFDAESAKIAA
LRILELGAKNVLVKGIHIKEGKIMDLLISQEGTTMEFQNEVIRINITHGSGCRLSTAIACHMSMGKSLRESIHLASDLLR
NEIIRMGVLYSTDTWL

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=255, Percent_Identity=32.156862745098, Blast_Score=111, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325378, Length=260, Percent_Identity=30.7692307692308, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324997, Length=260, Percent_Identity=31.9230769230769, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324517, Length=260, Percent_Identity=31.1538461538462, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27339; Mature: 27339

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIPEVCIVAGSDSIGGAGLQADLRIAALLECSASNVVTCITAQDLRGVHAIQYLSGEI
CCCCCCEEEEECCCCCCCCCCCHHHEEEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHHH
VSKQLICALSRKPKAVKIGMLGNSEIALVVYDVLKDAGIPIVLDPVLISTSNSKLADEGA
HHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEHHHHCCCCEEECCEEEECCCCCCCHHHH
VQVLLKKLISISHLVTPNLFEAGVLSGSKVFDAESAKIAALRILELGAKNVLVKGIHIKE
HHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCCHHEEHHHHHHCCCCEEEEEEEECC
GKIMDLLISQEGTTMEFQNEVIRINITHGSGCRLSTAIACHMSMGKSLRESIHLASDLLR
CCEEEEEECCCCCEEEECCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIIRMGVLYSTDTWL
HHHHHHEEEEECCCCC
>Mature Secondary Structure
MRKIPEVCIVAGSDSIGGAGLQADLRIAALLECSASNVVTCITAQDLRGVHAIQYLSGEI
CCCCCCEEEEECCCCCCCCCCCHHHEEEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHHH
VSKQLICALSRKPKAVKIGMLGNSEIALVVYDVLKDAGIPIVLDPVLISTSNSKLADEGA
HHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEHHHHCCCCEEECCEEEECCCCCCCHHHH
VQVLLKKLISISHLVTPNLFEAGVLSGSKVFDAESAKIAALRILELGAKNVLVKGIHIKE
HHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCCHHEEHHHHHHCCCCEEEEEEEECC
GKIMDLLISQEGTTMEFQNEVIRINITHGSGCRLSTAIACHMSMGKSLRESIHLASDLLR
CCEEEEEECCCCCEEEECCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
NEIIRMGVLYSTDTWL
HHHHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA