Click here to switch to the map view.

The map label for this gene is gyrA

Identifier: 88608652

GI number: 88608652

Start: 842498

End: 845107

Strand: Direct

Name: gyrA

Synonym: NSE_0958

Alternate gene names: 88608652

Gene position: 842498-845107 (Clockwise)

Preceding gene: 88608458

Following gene: 88608316

Centisome position: 98.08

GC content: 42.03

Gene sequence:

>2610_bases
ATGTCGACCATAATACCGGTAAATCTAGAGCAGGAACTGAAATCCTCTTACCTCTCTTACGCAATGAGCGTAATTATAAA
CAGGGCCATTCCCGATGTACGAGACGGATTAAAACCTGTACATAGGAGAATTCTGTATTCTATGAATGAGCTTTCTTTCT
ACTGGAACAAGCCTTATAGGAAGTCGGCTAGAGTGATTGGGGAGGTTATTGGTAAATATCACCCGCATGGTGATAGTGCT
ATATATGATGCTCTGGTTCGTATGGCACAGAACTTTTCCCTACTCGTACCATTGATAGACGGTCAGGGAAACTTTGGTTC
TATAGATGGTGATCCGGCTGCAGCGATGCGCTACACAGAAGTACGTCTGGAGTATATATCCAAGTTTCTACTCGACGATC
TGTATGAAAACAGTGTGGACTTCCGTTCAAACTATGACAACTCAGAAACGGAGCCTGTTGTTCTGCCGGCAAAATTCCCA
AATATCCTGGTCAATGGAACAGCTGGGGTAGCAGTCGGAATGGCAACGAATGTGCCAACTCACAACCTATCCGAGGTAAT
AGAGGCGTGCAAGTTGTACTTAGAAAACAATAACGTAACAACCGACGAACTCCTAAAAGTCATAAAAGGTCCCGACTTCC
CTACAGGTGGATTGATCATCGGCGAGGAAGGAATAAAGGCATCTTTTGAGAGCGGTAGAGGCTCCATTATCATACGGAGC
AAGACACATTTCGAAGAAACTGAAGCGGGAAAAGCAATTATCGTTGATGAGCTCCCGTACCAGGTTAATAAAGCAAAGTT
AATAGAAAACATAGCTCTTTTGGTGAAGGAAAAGAGACTTGATTCGATCAGTGATCTGAGAGACGAGTCAAACAAGGAAG
GGATACGTGTTGTAATAGAGTTAAAAAGAGGCGCCTCCTTTGAAGTGGTTCTGAACCAACTTTTCCAGATGACACAGCTT
CAGACATCATACAGCACGAATATGGTGCTTCTTGACAACATGAGACCTACTCTGATGTCTCTCAGAGACATTTTGGCAGC
CTTCATTGCTCATAGAAAAGAAGTAATCTCAAGACGAACAGAATTCAGACTCGCCAAGGCTAGGGAAAAAGCTAACGTTC
TAGTCGCACTGTATGTAGCGATTCTCAACATTGATGAGGTTGTACATATTTTAAAATCCTCTGGAGATAACGAGGCTGCA
ATAAAAGCACTTTCTGCTAAACACTGGGCTATAGACGAAGAACTTGAGCTACTTGTAGAAACTATCTCAAACATTAAGCT
CCTGAACAGGGAATACAAGCTCAGTACTGCGCAATCTAAGGCAATCTTAGAGATGAAAATGCAACGTATAACCAATACCG
AAAAAGGACAATTGGTCGCTGAGATTCATGCTGAAATGGACATTGTCAAAGAATGTGCCTCAATACTGGGAAGTGAAGCT
GTTTTGACAAATGTGATAGTGCGGGAACTAAATGAAATAAAGGCAAAGTACCCACAAGCACGGAAGAGTATAATTTTAAA
AAATGTCAACGCCGTGTACGCAGAAGAGGATCTTATCGAGCGTGAGCAAGTCGTCGTTACAGCGACATATTCTGGGTATA
TAAAGCGTGTCCACATCTCAAATTACAGGGTTCAGAGAAGAGGAGGTAAGGGGCGTATCGCACAAAACATAAAATCTGAG
GATCAGTTGGCACAGATTTTCGTTCTCAGTACGCATGATGAGATCCTCTTCTTTTCCAGTAGAGGTAAAGTCTATAAGTT
AAAAGCGTACAAGTTGCCTATAGGTGAACCTCAATCGCGCGGAAGAGCTTTGGTGAACATTTTTGATCTTGCTGATGATG
AGTCTATTACGAGCATTCTTCCTTTGAAAAGCTCCGATGCTGAGAACTTAATATTCTTGACTGCTCAAGGAAAGGTCAAA
CGCAATTCTATGAGTGACTTTGCCTATGTCCCAGCAAATGGAAAGGTAGCTATAAGGCTCAACGATAACGACGCATTAAT
ATCTGTCAAAACTGCTCAAAATGAAGACTGCGTTTTTATTTCCACCAGGGATGGAAAATGTATAAGGTTCTTGGTTGGGG
ATTTGAGAGTCCTTAAGAGTAGAAGTTCTGAAGGTGTAAAAGCAATAACCCTAGGAAAAGATGATAACGCTATTTCCCTC
TCAATACTGAATAAGCAGGTTCTTTCACCAGAGGACAAAGCTGAGTACATGAAAACTAAGTTCTTCGAGAGAATCCAAAA
TCCAGAAAATCATGAAGAACAGTTTGTTCTTACAGTTACCGAAAATGGTTATGGCAAAATCACGTCGAGTTATGAGTATA
GGATAACAAACCGAGGAGGCGTTGGGATAATCAATATATCGACTTCCGAGCGCAACGGTAAGGTGGTCTCGAGCTTCAAG
GTGACACTTAGTAACCATATAATGCTTGTTACAAATAGAGGACAGGTCATAAGGATAGAAGCAAACAACATCCCGGTCCT
TGGTCGTAACACACAAGGAGTACGTCTATTTTCCATCAGGGCTGAGGAAAGGGTTGCTTCGGTGACAGAAGTAGAAGAGG
TGGAAGATGATGACTCTGAAACAACTGAAAATTGCCAAGACAATAATTAG

Upstream 100 bases:

>100_bases
AACATTTCGGTGAAAGCTATGTCATCTCGCAAATACTTTAAAATGGAGTGACTATAAATATCGGACGAATTAACTTATCA
TTTCCCTCGGAGTAGTGCGT

Downstream 100 bases:

>100_bases
GCTGAATCCTTACTTCACCAGCTGTCTGCACAGAAGAAATGGAAACTGAGGATTAAAAGATAGCTAAAAGTTGTTGAAAC
GCATTGGGGGCTTATATTTA

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 869; Mature: 868

Protein sequence:

>869_residues
MSTIIPVNLEQELKSSYLSYAMSVIINRAIPDVRDGLKPVHRRILYSMNELSFYWNKPYRKSARVIGEVIGKYHPHGDSA
IYDALVRMAQNFSLLVPLIDGQGNFGSIDGDPAAAMRYTEVRLEYISKFLLDDLYENSVDFRSNYDNSETEPVVLPAKFP
NILVNGTAGVAVGMATNVPTHNLSEVIEACKLYLENNNVTTDELLKVIKGPDFPTGGLIIGEEGIKASFESGRGSIIIRS
KTHFEETEAGKAIIVDELPYQVNKAKLIENIALLVKEKRLDSISDLRDESNKEGIRVVIELKRGASFEVVLNQLFQMTQL
QTSYSTNMVLLDNMRPTLMSLRDILAAFIAHRKEVISRRTEFRLAKAREKANVLVALYVAILNIDEVVHILKSSGDNEAA
IKALSAKHWAIDEELELLVETISNIKLLNREYKLSTAQSKAILEMKMQRITNTEKGQLVAEIHAEMDIVKECASILGSEA
VLTNVIVRELNEIKAKYPQARKSIILKNVNAVYAEEDLIEREQVVVTATYSGYIKRVHISNYRVQRRGGKGRIAQNIKSE
DQLAQIFVLSTHDEILFFSSRGKVYKLKAYKLPIGEPQSRGRALVNIFDLADDESITSILPLKSSDAENLIFLTAQGKVK
RNSMSDFAYVPANGKVAIRLNDNDALISVKTAQNEDCVFISTRDGKCIRFLVGDLRVLKSRSSEGVKAITLGKDDNAISL
SILNKQVLSPEDKAEYMKTKFFERIQNPENHEEQFVLTVTENGYGKITSSYEYRITNRGGVGIINISTSERNGKVVSSFK
VTLSNHIMLVTNRGQVIRIEANNIPVLGRNTQGVRLFSIRAEERVASVTEVEEVEDDDSETTENCQDNN

Sequences:

>Translated_869_residues
MSTIIPVNLEQELKSSYLSYAMSVIINRAIPDVRDGLKPVHRRILYSMNELSFYWNKPYRKSARVIGEVIGKYHPHGDSA
IYDALVRMAQNFSLLVPLIDGQGNFGSIDGDPAAAMRYTEVRLEYISKFLLDDLYENSVDFRSNYDNSETEPVVLPAKFP
NILVNGTAGVAVGMATNVPTHNLSEVIEACKLYLENNNVTTDELLKVIKGPDFPTGGLIIGEEGIKASFESGRGSIIIRS
KTHFEETEAGKAIIVDELPYQVNKAKLIENIALLVKEKRLDSISDLRDESNKEGIRVVIELKRGASFEVVLNQLFQMTQL
QTSYSTNMVLLDNMRPTLMSLRDILAAFIAHRKEVISRRTEFRLAKAREKANVLVALYVAILNIDEVVHILKSSGDNEAA
IKALSAKHWAIDEELELLVETISNIKLLNREYKLSTAQSKAILEMKMQRITNTEKGQLVAEIHAEMDIVKECASILGSEA
VLTNVIVRELNEIKAKYPQARKSIILKNVNAVYAEEDLIEREQVVVTATYSGYIKRVHISNYRVQRRGGKGRIAQNIKSE
DQLAQIFVLSTHDEILFFSSRGKVYKLKAYKLPIGEPQSRGRALVNIFDLADDESITSILPLKSSDAENLIFLTAQGKVK
RNSMSDFAYVPANGKVAIRLNDNDALISVKTAQNEDCVFISTRDGKCIRFLVGDLRVLKSRSSEGVKAITLGKDDNAISL
SILNKQVLSPEDKAEYMKTKFFERIQNPENHEEQFVLTVTENGYGKITSSYEYRITNRGGVGIINISTSERNGKVVSSFK
VTLSNHIMLVTNRGQVIRIEANNIPVLGRNTQGVRLFSIRAEERVASVTEVEEVEDDDSETTENCQDNN
>Mature_868_residues
STIIPVNLEQELKSSYLSYAMSVIINRAIPDVRDGLKPVHRRILYSMNELSFYWNKPYRKSARVIGEVIGKYHPHGDSAI
YDALVRMAQNFSLLVPLIDGQGNFGSIDGDPAAAMRYTEVRLEYISKFLLDDLYENSVDFRSNYDNSETEPVVLPAKFPN
ILVNGTAGVAVGMATNVPTHNLSEVIEACKLYLENNNVTTDELLKVIKGPDFPTGGLIIGEEGIKASFESGRGSIIIRSK
THFEETEAGKAIIVDELPYQVNKAKLIENIALLVKEKRLDSISDLRDESNKEGIRVVIELKRGASFEVVLNQLFQMTQLQ
TSYSTNMVLLDNMRPTLMSLRDILAAFIAHRKEVISRRTEFRLAKAREKANVLVALYVAILNIDEVVHILKSSGDNEAAI
KALSAKHWAIDEELELLVETISNIKLLNREYKLSTAQSKAILEMKMQRITNTEKGQLVAEIHAEMDIVKECASILGSEAV
LTNVIVRELNEIKAKYPQARKSIILKNVNAVYAEEDLIEREQVVVTATYSGYIKRVHISNYRVQRRGGKGRIAQNIKSED
QLAQIFVLSTHDEILFFSSRGKVYKLKAYKLPIGEPQSRGRALVNIFDLADDESITSILPLKSSDAENLIFLTAQGKVKR
NSMSDFAYVPANGKVAIRLNDNDALISVKTAQNEDCVFISTRDGKCIRFLVGDLRVLKSRSSEGVKAITLGKDDNAISLS
ILNKQVLSPEDKAEYMKTKFFERIQNPENHEEQFVLTVTENGYGKITSSYEYRITNRGGVGIINISTSERNGKVVSSFKV
TLSNHIMLVTNRGQVIRIEANNIPVLGRNTQGVRLFSIRAEERVASVTEVEEVEDDDSETTENCQDNN

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=352, Percent_Identity=25.8522727272727, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI19913406, Length=355, Percent_Identity=25.0704225352113, Blast_Score=78, Evalue=4e-14,
Organism=Escherichia coli, GI1788562, Length=876, Percent_Identity=45.8904109589041, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=706, Percent_Identity=33.7110481586402, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535065, Length=365, Percent_Identity=26.5753424657534, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI212645845, Length=376, Percent_Identity=25, Blast_Score=83, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI212645657, Length=440, Percent_Identity=25.6818181818182, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=173, Percent_Identity=30.635838150289, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136538, Length=367, Percent_Identity=25.3405994550409, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 97299; Mature: 97167

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIPVNLEQELKSSYLSYAMSVIINRAIPDVRDGLKPVHRRILYSMNELSFYWNKPYR
CCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHH
KSARVIGEVIGKYHPHGDSAIYDALVRMAQNFSLLVPLIDGQGNFGSIDGDPAAAMRYTE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
VRLEYISKFLLDDLYENSVDFRSNYDNSETEPVVLPAKFPNILVNGTAGVAVGMATNVPT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECCCCC
HNLSEVIEACKLYLENNNVTTDELLKVIKGPDFPTGGLIIGEEGIKASFESGRGSIIIRS
CCHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEE
KTHFEETEAGKAIIVDELPYQVNKAKLIENIALLVKEKRLDSISDLRDESNKEGIRVVIE
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
LKRGASFEVVLNQLFQMTQLQTSYSTNMVLLDNMRPTLMSLRDILAAFIAHRKEVISRRT
ECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFRLAKAREKANVLVALYVAILNIDEVVHILKSSGDNEAAIKALSAKHWAIDEELELLVE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHH
TISNIKLLNREYKLSTAQSKAILEMKMQRITNTEKGQLVAEIHAEMDIVKECASILGSEA
HHHHHHHHCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHH
VLTNVIVRELNEIKAKYPQARKSIILKNVNAVYAEEDLIEREQVVVTATYSGYIKRVHIS
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCHHHHHCCCEEEEEEECCCEEEEEEEC
NYRVQRRGGKGRIAQNIKSEDQLAQIFVLSTHDEILFFSSRGKVYKLKAYKLPIGEPQSR
CEEEEECCCCCHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCEEEEEEEECCCCCCCCC
GRALVNIFDLADDESITSILPLKSSDAENLIFLTAQGKVKRNSMSDFAYVPANGKVAIRL
CCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCEEEEEE
NDNDALISVKTAQNEDCVFISTRDGKCIRFLVGDLRVLKSRSSEGVKAITLGKDDNAISL
CCCCEEEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
SILNKQVLSPEDKAEYMKTKFFERIQNPENHEEQFVLTVTENGYGKITSSYEYRITNRGG
EEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCEEEEEECCCC
VGIINISTSERNGKVVSSFKVTLSNHIMLVTNRGQVIRIEANNIPVLGRNTQGVRLFSIR
EEEEEEECCCCCCCEEEEEEEEECCEEEEEECCCCEEEEECCCCEEECCCCCCEEEEEEE
AEERVASVTEVEEVEDDDSETTENCQDNN
HHHHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure 
STIIPVNLEQELKSSYLSYAMSVIINRAIPDVRDGLKPVHRRILYSMNELSFYWNKPYR
CCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEECCCHH
KSARVIGEVIGKYHPHGDSAIYDALVRMAQNFSLLVPLIDGQGNFGSIDGDPAAAMRYTE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
VRLEYISKFLLDDLYENSVDFRSNYDNSETEPVVLPAKFPNILVNGTAGVAVGMATNVPT
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECCCCC
HNLSEVIEACKLYLENNNVTTDELLKVIKGPDFPTGGLIIGEEGIKASFESGRGSIIIRS
CCHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEE
KTHFEETEAGKAIIVDELPYQVNKAKLIENIALLVKEKRLDSISDLRDESNKEGIRVVIE
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
LKRGASFEVVLNQLFQMTQLQTSYSTNMVLLDNMRPTLMSLRDILAAFIAHRKEVISRRT
ECCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EFRLAKAREKANVLVALYVAILNIDEVVHILKSSGDNEAAIKALSAKHWAIDEELELLVE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHH
TISNIKLLNREYKLSTAQSKAILEMKMQRITNTEKGQLVAEIHAEMDIVKECASILGSEA
HHHHHHHHCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHH
VLTNVIVRELNEIKAKYPQARKSIILKNVNAVYAEEDLIEREQVVVTATYSGYIKRVHIS
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCHHHHHCCCEEEEEEECCCEEEEEEEC
NYRVQRRGGKGRIAQNIKSEDQLAQIFVLSTHDEILFFSSRGKVYKLKAYKLPIGEPQSR
CEEEEECCCCCHHHHHCCCCCCEEEEEEEECCCCEEEECCCCCEEEEEEEECCCCCCCCC
GRALVNIFDLADDESITSILPLKSSDAENLIFLTAQGKVKRNSMSDFAYVPANGKVAIRL
CCEEEEEEECCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCEEEECCCCEEEEEE
NDNDALISVKTAQNEDCVFISTRDGKCIRFLVGDLRVLKSRSSEGVKAITLGKDDNAISL
CCCCEEEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
SILNKQVLSPEDKAEYMKTKFFERIQNPENHEEQFVLTVTENGYGKITSSYEYRITNRGG
EEHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECCEEEEEECCCC
VGIINISTSERNGKVVSSFKVTLSNHIMLVTNRGQVIRIEANNIPVLGRNTQGVRLFSIR
EEEEEEECCCCCCCEEEEEEEEECCEEEEEECCCCEEEEECCCCEEECCCCCCEEEEEEE
AEERVASVTEVEEVEDDDSETTENCQDNN
HHHHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA