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The map label for this gene is ruvA

Identifier: 88608038

GI number: 88608038

Start: 500346

End: 500927

Strand: Direct

Name: ruvA

Synonym: NSE_0574

Alternate gene names: 88608038

Gene position: 500346-500927 (Clockwise)

Preceding gene: 88608436

Following gene: 88608213

Centisome position: 58.25

GC content: 38.49

Gene sequence:

>582_bases
ATGATAGGTAAACTCACAGGCACGGTAACAGACCTATGCCAAGAATCTCTTATATTAGACGTAGGCGGAGTAGGATATAA
CGTCTTTACAACACGCAGACTCATTGATAGTCTTCGTACTGGGCAAAATCTATCTTTGTACATAGAACATTATTTCTTGG
AGAACATAAACAAACTTTACGGGTTTGAATGCAGAAAATCGCAAGAAGTAGCAAGAATGCTGAGCAAAGTAAAGGGAATA
AACTACAAGATTGCTCTTAGTCTGCTAAATCATCTCGAACTAGGAGAGTTAATCCTAGCAATTCAAAATAAAGATGAATC
CCGCTTAAAAATCAAAGGTATAGGAGAGAAACTAGTAAAACGCATTATCACAGAAACGTATGAAGACTTCTTGAAGTTAG
ACTCACACCTTTCTGGAATTGCTTCCTCGACAAATGTACATATAGCAAGTGAGGCCGTATCTGCTCTAGTAAAGCTAGGT
TTTCAACATAAACCCTCCCACAAAGTGGTCATGGAAATCATGACAAAACGACCTGCAATTGAAATAGCCGAACTAATCAC
GCTAGCGCTCAAAATGCTGTAA

Upstream 100 bases:

>100_bases
TCGAGAAAGCACATAAACACCTTTTGCGCTTAATCTGTCACTGGATTCACTCTTTATGGAGTTACTTTATTTATTTCATC
GCTGGAATCTTTCTTCCAAT

Downstream 100 bases:

>100_bases
TCTTTGTGTAATCTACCAACTTGTCATTTCTGTTAAAGTCATGTTCGGTAAACCAGCAACATACTCTCATTACAATGATC
ATTTCAACAATAAAGAAATA

Product: holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MIGKLTGTVTDLCQESLILDVGGVGYNVFTTRRLIDSLRTGQNLSLYIEHYFLENINKLYGFECRKSQEVARMLSKVKGI
NYKIALSLLNHLELGELILAIQNKDESRLKIKGIGEKLVKRIITETYEDFLKLDSHLSGIASSTNVHIASEAVSALVKLG
FQHKPSHKVVMEIMTKRPAIEIAELITLALKML

Sequences:

>Translated_193_residues
MIGKLTGTVTDLCQESLILDVGGVGYNVFTTRRLIDSLRTGQNLSLYIEHYFLENINKLYGFECRKSQEVARMLSKVKGI
NYKIALSLLNHLELGELILAIQNKDESRLKIKGIGEKLVKRIITETYEDFLKLDSHLSGIASSTNVHIASEAVSALVKLG
FQHKPSHKVVMEIMTKRPAIEIAELITLALKML
>Mature_193_residues
MIGKLTGTVTDLCQESLILDVGGVGYNVFTTRRLIDSLRTGQNLSLYIEHYFLENINKLYGFECRKSQEVARMLSKVKGI
NYKIALSLLNHLELGELILAIQNKDESRLKIKGIGEKLVKRIITETYEDFLKLDSHLSGIASSTNVHIASEAVSALVKLG
FQHKPSHKVVMEIMTKRPAIEIAELITLALKML

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=202, Percent_Identity=28.7128712871287, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_NEOSM (Q2GDJ1)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506455.1
- ProteinModelPortal:   Q2GDJ1
- SMR:   Q2GDJ1
- STRING:   Q2GDJ1
- GeneID:   3931494
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0574
- TIGR:   NSE_0574
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   YKTAMSI
- ProtClustDB:   CLSK2527672
- BioCyc:   NSEN222891:NSE_0574-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21627; Mature: 21627

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKLTGTVTDLCQESLILDVGGVGYNVFTTRRLIDSLRTGQNLSLYIEHYFLENINKLY
CCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GFECRKSQEVARMLSKVKGINYKIALSLLNHLELGELILAIQNKDESRLKIKGIGEKLVK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCHHHHHH
RIITETYEDFLKLDSHLSGIASSTNVHIASEAVSALVKLGFQHKPSHKVVMEIMTKRPAI
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
EIAELITLALKML
HHHHHHHHHHHHC
>Mature Secondary Structure
MIGKLTGTVTDLCQESLILDVGGVGYNVFTTRRLIDSLRTGQNLSLYIEHYFLENINKLY
CCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GFECRKSQEVARMLSKVKGINYKIALSLLNHLELGELILAIQNKDESRLKIKGIGEKLVK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCHHHHHH
RIITETYEDFLKLDSHLSGIASSTNVHIASEAVSALVKLGFQHKPSHKVVMEIMTKRPAI
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHH
EIAELITLALKML
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA